LOCUS       pCAG-NLS-HA-Bxb1      6378 bp    DNA     circular 
FEATURES             Location/Qualifiers
     Promoter        join(6371..6378,1..1660)
                     /label=CAG Promoter
     Promoter        1670..1692
                     /label=T7
     CDS             1708..3264
                     /label=Bxb1
     Signal_peptide  1711..1731
                     /label=SV40 NLS
     Tag             1732..1764
                     /label=HA
     PolyA_signal    3272..3542
                     /label=BGHpA
     CDS             4330..5190
                     /label=Amp
ORIGIN
        1 TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG
       61 GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC
      121 CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT
      181 TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT
      241 CATATGCCAA GTACGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT
      301 GCCCAGTACA TGACCTTATG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC
      361 GCTATTACCA TGGTCGAGGT GAGCCCCACG TTCTGCTTCA CTCTCCCCAT CTCCCCCCCC
      421 TCCCCACCCC CAATTTTGTA TTTATTTATT TTTTAATTAT TTTGTGCAGC GATGGGGGCG
      481 GGGGGGGGGG GGGGGCGCGC GCCAGGCGGG GCGGGGCGGG GCGAGGGGCG GGGCGGGGCG
      541 AGGCGGAGAG GTGCGGCGGC AGCCAATCAG AGCGGCGCGC TCCGAAAGTT TCCTTTTATG
      601 GCGAGGCGGC GGCGGCGGCG GCCCTATAAA AAGCGAAGCG CGCGGCGGGC GGGAGTCGCT
      661 GCGTTGCCTT CGCCCCGTGC CCCGCTCCGC GCCGCCTCGC GCCGCCCGCC CCGGCTCTGA
      721 CTGACCGCGT TACTCCCACA GGTGAGCGGG CGGGACGGCC CTTCTCCTCC GGGCTGTAAT
      781 TAGCGCTTGG TTTAATGACG GCTCGTTTCT TTTCTGTGGC TGCGTGAAAG CCTTAAAGGG
      841 CTCCGGGAGG GCCCTTTGTG CGGGGGGGAG CGGCTCGGGG GGTGCGTGCG TGTGTGTGTG
      901 CGTGGGGAGC GCCGCGTGCG GCCCGCGCTG CCCGGCGGCT GTGAGCGCTG CGGGCGCGGC
      961 GCGGGGCTTT GTGCGCTCCG CGTGTGCGCG AGGGGAGCGC GGCCGGGGGC GGTGCCCCGC
     1021 GGTGCGGGGG GGCTGCGAGG GGAACAAAGG CTGCGTGCGG GGTGTGTGCG TGGGGGGGTG
     1081 AGCAGGGGGT GTGGGCGCGG CGGTCGGGCT GTAACCCCCC CCTGCACCCC CCTCCCCGAG
     1141 TTGCTGAGCA CGGCCCGGCT TCGGGTGCGG GGCTCCGTGC GGGGCGTGGC GCGGGGCTCG
     1201 CCGTGCCGGG CGGGGGGTGG CGGCAGGTGG GGGTGCCGGG CGGGGCGGGG CCGCCTCGGG
     1261 CCGGGGAGGG CTCGGGGGAG GGGCGCGGCG GCCCCGGAGC GCCGGCGGCT GTCGAGGCGC
     1321 GGCGAGCCGC AGCCATTGCC TTTTATGGTA ATCGTGCGAG AGGGCGCAGG GACTTCCTTT
     1381 GTCCCAAATC TGGCGGAGCC GAAATCTGGG AGGCGCCGCC GCACCCCCTC TAGCGGGCGC
     1441 GGGCGAAGCG GTGCGGCGCC GGCAGGAAGG AAATGGGCGG GGAGGGCCTT CGTGCGTCGC
     1501 CGCGCCGCCG TCCCCTTCTC CATCTCCAGC CTCGGGGCTG CCGCAGGGGG ACGGCTGCCT
     1561 TCGGGGGGGA CGGGGCAGGG CGGGGTTCGG CTTCTGGCGT GTGACCGGCG GCTCTAGAGC
     1621 CTCTGCTAAC CATGTTCATG CCTTCTTCTT TTTCCTACAG ATCCTTAATT AATAATACGA
     1681 CTCACTATAG GGGGTCGACC CGCCACCATG CCAAAAAAGA AAAGAAAAGT GTATCCCTAT
     1741 GATGTCCCCG ATTATGCCGG TTCAAGAGCC CTGGTCGTGA TTAGACTGAG CCGAGTGACA
     1801 GACGCCACCA CAAGTCCCGA GAGACAGCTG GAATCATGCC AGCAGCTCTG TGCTCAGCGG
     1861 GGTTGGGATG TGGTCGGCGT GGCAGAGGAT CTGGACGTGA GCGGGGCCGT CGATCCATTC
     1921 GACAGAAAGA GGAGGCCCAA CCTGGCAAGA TGGCTCGCTT TCGAGGAACA GCCCTTTGAT
     1981 GTGATCGTCG CCTACAGAGT GGACCGGCTG ACCCGCTCAA TTCGACATCT CCAGCAGCTG
     2041 GTGCATTGGG CTGAGGACCA CAAGAAACTG GTGGTCAGCG CAACAGAAGC CCACTTCGAT
     2101 ACTACCACAC CTTTTGCCGC TGTGGTCATC GCACTGATGG GCACTGTGGC CCAGATGGAG
     2161 CTCGAAGCTA TCAAGGAGCG AAACAGGAGC GCAGCCCATT TCAATATTAG GGCCGGTAAA
     2221 TACAGAGGCT CCCTGCCCCC TTGGGGATAT CTCCCTACCA GGGTGGATGG GGAGTGGAGA
     2281 CTGGTGCCAG ACCCCGTCCA GAGAGAGCGG ATTCTGGAAG TGTACCACAG AGTGGTCGAT
     2341 AACCACGAAC CACTCCATCT GGTGGCACAC GACCTGAATA GACGCGGCGT GCTCTCTCCA
     2401 AAGGATTATT TTGCTCAGCT GCAGGGAAGA GAGCCACAGG GAAGAGAATG GAGTGCTACT
     2461 GCACTGAAGA GATCTATGAT CAGTGAGGCT ATGCTGGGTT ACGCAACACT CAATGGCAAA
     2521 ACTGTCCGGG ACGATGACGG AGCCCCTCTG GTGAGGGCTG AGCCTATTCT CACCAGAGAG
     2581 CAGCTCGAAG CTCTGCGGGC AGAACTGGTC AAGACTAGTC GCGCCAAACC TGCCGTGAGC
     2641 ACCCCAAGCC TGCTCCTGAG GGTGCTGTTC TGCGCCGTCT GTGGAGAGCC AGCATACAAG
     2701 TTTGCCGGCG GAGGGCGCAA ACATCCCCGC TATCGATGCA GGAGCATGGG GTTCCCTAAG
     2761 CACTGTGGAA ACGGGACAGT GGCCATGGCT GAGTGGGACG CCTTTTGCGA GGAACAGGTG
     2821 CTGGATCTCC TGGGTGACGC TGAGCGGCTG GAAAAAGTGT GGGTGGCAGG ATCTGACTCC
     2881 GCTGTGGAGC TGGCAGAAGT CAATGCCGAG CTCGTGGATC TGACTTCCCT CATCGGATCT
     2941 CCTGCATATA GAGCTGGGTC CCCACAGAGA GAAGCTCTGG ACGCACGAAT TGCTGCACTC
     3001 GCTGCTAGAC AGGAGGAACT GGAGGGCCTG GAGGCCAGGC CCTCTGGATG GGAGTGGCGA
     3061 GAAACCGGAC AGAGGTTTGG GGATTGGTGG AGGGAGCAGG ACACCGCAGC CAAGAACACA
     3121 TGGCTGAGAT CCATGAATGT CCGGCTCACA TTCGACGTGC GCGGTGGCCT GACTCGAACC
     3181 ATCGATTTTG GCGACCTGCA GGAGTATGAA CAGCACCTGA GACTGGGGTC CGTGGTCGAA
     3241 AGACTGCACA CTGGGATGTC CTAGgtcaGA GCTCGCTGAT CAGCCTCGAC TGTGCCTTCT
     3301 AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC
     3361 ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT
     3421 CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT
     3481 AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG AACCAGCTGG
     3541 GGCTCGAGAT CCACTAGTTC TAGCCTCGAG GCTAGAGCGG CCGCCACTGG CCGTCGTTTT
     3601 ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC
     3661 CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GATCGCCCTT CCCAACAGTT
     3721 GCGCAGCCTG AATGGCGAAT GGGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT
     3781 GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC
     3841 TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG
     3901 GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA
     3961 GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT
     4021 GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT
     4081 CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA
     4141 TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA ATATTAACGC TTACAATTTA
     4201 GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT
     4261 TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA
     4321 AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT
     4381 TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG
     4441 TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT
     4501 TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG
     4561 GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG
     4621 AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA
     4681 AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG
     4741 ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA
     4801 ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC
     4861 ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT
     4921 ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA
     4981 CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG
     5041 CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA
     5101 GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG
     5161 ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT
     5221 TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT
     5281 AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA
     5341 GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA
     5401 ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT
     5461 TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG
     5521 CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA
     5581 ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA
     5641 AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG
     5701 CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA
     5761 AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA
     5821 ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC
     5881 GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC
     5941 CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT
     6001 GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT
     6061 GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG
     6121 GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA
     6181 TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT
     6241 GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG
     6301 TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGAGGCG
     6361 CGCCGGATTC GACATTGA
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