LOCUS       48647\DS-ST1cas         6028 bp    DNA     circular     20-SEP-2013
DEFINITION  .
SOURCE      .
  ORGANISM  .
COMMENT     
            ApEinfo:methylated:1
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|673607993|
COMMENT     VNTDBDATE|673607993|
COMMENT     LSOWNER|
COMMENT     VNTNAME|48647 DS-ST1cas|
COMMENT     VNTAUTHORNAME|Kevin Esvelt|
COMMENT     VNTAUTHOREML|kesvelt@gmail.com|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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FEATURES             Location/Qualifiers
     terminator      107..194
                     /vntifkey="43"
                     /label=rrnBT2\term
                     /note="near-perfect bidirectional terminator"
     promoter        205..364
                     /vntifkey="30"
                     /label=proC
     rep_origin      4284..5022
                     /vntifkey="33"
                     /label=cloD13\(CDF)
     CDS             5162..5950
                     /vntifkey="4"
                     /label=aadA
                     /note="spectinomycin resistance"
     CDS             377..3742
                     /vntifkey="4"
                     /label=ST1\cas9
     misc_feature    3756..3966
                     /vntifkey="21"
                     /label=ST1\tracrRNA
     misc_feature    401..403
                     /vntifkey="21"
                     /label=catalytic\Asp
     misc_feature    2240..2242
                     /vntifkey="21"
                     /label=catalytic\Asn
     misc_feature    2168..2173
                     /vntifkey="21"
                     /label=catalytic\Asp\His
BASE COUNT     1576 a      1722 c      1687 g      1043 t 
ORIGIN
        1 accaggcacg cctaaccgtc agtgagattg gatgagtgaa cgatattgat cgagaagagc 
       61 cctgcgcagc cgctgccgtg cctgcaggaa gcaacggccc ggagggtggc gggcaggacg 
      121 cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt 
      181 tgcgtttcta caaactctgc tagcttctag agcacagcta acaccacgtc gtccctatct 
      241 gctgccctag gtctatgagt ggttgctgga taactttacg ggcatgcata aggctcgtat 
      301 gatatattca gggagaccac aacggtttcc ctctacaaat aattttgttt aacttttact 
      361 agaggaggag gcaaaaatga gcgacctggt gctgggcctg gacatcggca tcggcagcgt 
      421 gggcgtgggc atcctgaaca aggtgaccgg cgagatcatc cacaagaaca gtcgcatctt 
      481 ccctgctgct caggctgaga acaacctggt gcgccgcacc aaccgccagg gtcgccggct 
      541 tgctcgccgc aagaagcacc ggcgcgtgcg cctgaaccgc ctgttcgagg agagcggcct 
      601 gatcaccgac ttcaccaaga tcagcatcaa cctgaacccc taccagctgc gcgtgaaggg 
      661 cctgaccgac gagctgagca acgaggagct gttcatcgcc ctgaagaaca tggtgaagca 
      721 ccgcggcatc agctacctgg acgacgccag cgacgacggc aacagcagcg tgggcgacta 
      781 cgcccagatc gtgaaggaga acagcaagca gctggagacc aagacccccg gccagatcca 
      841 gctggagcgc taccagacct acggccagct gcgcggcgac ttcaccgtgg agaaggacgg 
      901 caagaagcac cgcctgatca acgtgttccc caccagcgcc taccgcagcg aggccctgcg 
      961 catcctgcag acccagcagg agttcaaccc ccagatcacc gacgagttca tcaaccgcta 
     1021 cctggagatc ctgaccggca agcgcaagta ctaccacggc cccggcaacg agaagagccg 
     1081 caccgactac ggccgctacc gcaccagcgg cgagaccctg gacaacatct tcggcatcct 
     1141 gatcggcaag tgcaccttct accccgacga gttccgcgcc gccaaggcca gctacaccgc 
     1201 ccaggagttc aacctgctga acgacctgaa caacctgacc gtgcccaccg agaccaagaa 
     1261 gctgagcaag gagcagaaga accagatcat caactacgtg aagaacgaga aggccatggg 
     1321 ccccgccaag ctgttcaagt acatcgccaa gctgctgagc tgcgacgtgg ccgacatcaa 
     1381 gggctaccgc atcgacaaga gcggcaaggc cgagatccac accttcgagg cctaccgcaa 
     1441 gatgaagacc ctggagaccc tggacatcga gcagatggac cgcgagaccc tggacaagct 
     1501 ggcctacgtg ctgaccctga acaccgagcg cgagggcatc caggaggccc tggagcacga 
     1561 gttcgccgac ggcagcttca gccagaagca ggtggacgag ctggtgcagt tccgcaaggc 
     1621 caacagcagc atcttcggca agggctggca caacttcagc gtgaagctga tgatggagct 
     1681 gatccccgag ctgtacgaga ccagcgagga gcagatgacc atcctgaccc gcctgggcaa 
     1741 gcagaagacc accagcagca gcaacaagac caagtacatc gacgagaagc tgctgaccga 
     1801 ggagatctac aaccccgtgg tggccaagag cgtgcgccag gccatcaaga tcgtgaacgc 
     1861 cgccatcaag gagtacggcg acttcgacaa catcgtgatc gagatggccc gcgagaccaa 
     1921 cgaggacgac gagaagaagg ccatccagaa gatccagaag gccaacaagg acgagaagga 
     1981 cgccgccatg ctgaaggccg ccaaccagta caacggcaag gccgagctgc cccacagcgt 
     2041 gttccacggc cacaagcagc tggccaccaa gatccgcctg tggcaccagc agggcgagcg 
     2101 ctgcctgtac accggcaaga ccatcagcat ccacgacctg atcaacaaca gcaaccagtt 
     2161 cgaggtggac cacatcctgc ccctgagcat caccttcgac gacagcctgg ccaacaaggt 
     2221 gctggtgtac gccaccgcca accaggagaa gggccagcgc accccctacc aggccctgga 
     2281 cagcatggac gacgcctgga gcttccgcga gctgaaggcc ttcgtgcgcg agagcaagac 
     2341 cctgagcaac aagaagaagg agtacctgct gaccgaggag gacatcagca agttcgacgt 
     2401 gcgcaagaag ttcatcgagc gcaacctggt ggacacccgc tacgccagcc gcgtggtgct 
     2461 gaacgccctg caggagcact tccgcgccca caagatcgac accaaggtga gcgtggtgcg 
     2521 cggccagttc accagccagc tgcgccgcca ctggggcatc gagaagaccc gcgacaccta 
     2581 ccaccaccac gccgtggacg ccctgatcat tgcggcttct agccagctga acctgtggaa 
     2641 gaagcagaag aacaccctgg tgagctacag cgaggaccag ctgctggaca tcgagaccgg 
     2701 cgagctgatc agcgacgacg agtacaagga gagcgtgttc aaggccccct accagcactt 
     2761 cgtggacacc ctgaagagca aggagttcga ggacagcatc ctgttcagct accaggtgga 
     2821 cagcaagttc aaccgcaaga tcagcgacgc caccatctac gccacccgcc aggccaaggt 
     2881 gggcaaggac aaggccgacg agacctacgt gctgggcaag atcaaggaca tctacaccca 
     2941 ggacggctac gacgccttca tgaagatcta caagaaggac aagagcaagt tcctgatgta 
     3001 ccgccacgac ccccagacct tcgagaaggt gatcgagccc atcctggaga actaccccaa 
     3061 caagcagatc aacgataaag gcaaggaggt gccctgcaac cccttcctga agtacaagga 
     3121 ggagcacggc tacatccgca agtacagcaa gaagggcaac ggccccgaga tcaagagcct 
     3181 gaagtactac gacagcaagc tgggcaacca catcgacatc acccccaagg acagcaacaa 
     3241 caaggtggtg ctgcagagcg tgagcccctg gcgcgccgac gtgtacttca acaagaccac 
     3301 cggcaagtac gagatcctgg gcctgaagta cgccgacctg cagtttgata agggcaccgg 
     3361 cacctacaag atcagccagg agaagtacaa cgacatcaag aagaaggagg gcgtggacag 
     3421 cgacagcgag ttcaagttca ccctgtacaa gaacgacctt ctgctggtga aggacaccga 
     3481 gaccaaggag caacagctgt tccgcttcct gagccgcacc atgcccaagc agaagcacta 
     3541 cgtggagctg aagccctacg acaagcagaa gttcgagggc ggcgaggccc tgatcaaggt 
     3601 gctgggcaac gtggccaaca gcggccagtg caagaagggc ctgggcaaga gcaacatcag 
     3661 catctacaag gtgcgcaccg acgtgctggg caaccagcac atcatcaaga acgagggcga 
     3721 caagcccaag ctggacttct aacatccgcg gcaccgccta agctagattt ttaatattaa 
     3781 ttgttagaaa gtgttgcaat tatagttatc atatactata ataatagtgt aagggacgcc 
     3841 ttacacagtt acttaaatct tgcagaagct acaaagataa ggcttcatgc cgaaatcaac 
     3901 accctgtcat tttatggcag ggtgttttcg ttatttaaag aggagaagaa atgacttgga 
     3961 gagttgcctg caggctccca tcttgggaca atgaaaacgt tagtcatggc gcgccttgac 
     4021 ggctagctca gtcctaggta cagtgctagc ttaattagtc tacgaggttt tagagctatg 
     4081 ctgttttgaa tggtcccaaa acctgcagac aagcccggcc ggcctaaggc gatgccccct 
     4141 cgacctcgat cagggaggcg ttcaggacga ctcacaaaga aagccgggca atgcccggct 
     4201 ttttccacgc ctcctgggct gacttcaggt gctacatttg aagagataaa ttgcactgaa 
     4261 atctagagcg gttcagtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 
     4321 gtaatctttt gccctgtaaa cgaaaaaacc acctggggag gtggtttgat cgaaggttaa 
     4381 gtcagttggg gaactgctta accgtggtaa ctggctttcg cagagcacag caaccaaatc 
     4441 tgtccttcca gtgtagccgg actttggcgc acacttcaag agcaaccgcg tgtttagcta 
     4501 aacaaatcct ctgcgaactc ccagttacca atggctgctg ccagtggcgt tttaccgtgc 
     4561 ttttccgggt tggactcaag tgaacagtta ccggataagg cgcagcagtc gggctgaacg 
     4621 gggagttctt gcttacagcc cagcttggag cgaacgacct acaccgagcc gagataccag 
     4681 tgtgtgagct atgagaaagc gccacacttc ccgtaaggga gaaaggcgga acaggtatcc 
     4741 ggtaaacggc agggtcggaa caggagagcg caagagggag cgacccgccg gaaacggtgg 
     4801 ggatctttaa gtcctgtcgg gtttcgcccg tactgtcaga ttcatggttg agcctcacgg 
     4861 ctcccacaga tgcaccggaa aagcgtctgt ttatgtgaac tctggcagga gggcggagcc 
     4921 tatggaaaaa cgccaccggc gcggccctgc tgttttgcct cacatgttag tcccctgctt 
     4981 atccacggaa tctgtgggta actttgtatg tgtccgcagc gcccgccgca gtctcacgcc 
     5041 cggagcgtag cgaccgagtg agctagctat ttgtttattt ttctaaatac attcaaatat 
     5101 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 
     5161 tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat 
     5221 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg 
     5281 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga 
     5341 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag 
     5401 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg 
     5461 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg 
     5521 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga 
     5581 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca 
     5641 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc 
     5701 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg 
     5761 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta 
     5821 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc 
     5881 ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt 
     5941 agtcggcaaa taatgtctaa caattcgttc aagccgaggg gccgcaagat ccggccacga 
     6001 tgacccggtc gtcggttcag ggcagggt 
//
