LOCUS pMyNT 6896 bp ds-DNA circular SYN 27-MAR-2013 DEFINITION . ACCESSION . VERSION . KEYWORDS pMyNT SOURCE synthetic DNA construct ORGANISM . REFERENCE 1 (bases 1 to 6896) AUTHORS . TITLE Direct Submission JOURNAL Exported 27Mar, 2013 from SnapGene Viewer 1.5.2 http://www.snapgene.com COMMENT This file is created by Vector NTI http://www.invitrogen.com/ FEATURES Location/Qualifiers source 1..6896 /organism="synthetic DNA construct" /mol_type="plasmid" CDS complement(14..1012) /codon_start=1 /label="hygR" /note="hygR" /translation="VTQESLLLLDRIDSDDSYASLRNDQEFWEPLARRALEELGLPVPP VLRVPGESTNPVLVGEPDPVIKLFGEHWCGPESLASESEAYAVLADAPVPVPRLLGRGE LRPGTGAWPWPYLVMSRMTGTTWRSAMDGTTDRNALLALARELGRVLGRLHRVPLTGNT VLTPHSEVFPELLRERRAATVEDHRGWGYLSPRLLDRLEDWLPDVDTLLAGREPRFVHG DLHGTNIFVDLAATEVTGIVDFTDVYAGDSRYSLVQLHLNAFRGDREILAALLDGAQWK RTEDFARELLAFTFLHDFEVFEETPLDLSGFTDPEELAQFLWGPPDTAPGA" rep_origin 1280..1680 /direction=RIGHT /label="ColE1" /note="ColE1" misc_binding 2054..3135 /label="sequence-verified (primer AP330)" /note="sequence-verified" rep_origin 2826..3342 /direction=RIGHT /label="mycobact ori" /note="mycobact ori" misc_binding 3078..4062 /label="sequence-verified (primer AP329)" /note="sequence-verified" promoter 4046..6657 /label="acetamidase promoter" /note="acetamidase promoter" CDS complement(4046..5119) /codon_start=1 /transl_table=11 /gene="amiC" /product="acetamidase regulator" /label="amiC" /note="amiC" /note="AmiC" /db_xref="GI:31339872" /protein_id="DAA01131.1" /translation="VQDGEVEFRVGLVIPLQGPAGIFAPSCEAVAELAAKEVNDRGGLQ GRKVTIEVLDGGAPGDDVARTVADRLRGHGLDAVTGWHISAVRNRISPVVRDRIPYVYT SLYEGGERTPGVFCTGETPQIQIAPALAWLRDHFGIRSWCLVGDDYIWPRRSAAAARAY CRDLDLELRREIYVPYGTDDFRAPVRKAIASGAQAVLMLLVGQDAVLFNREFARAGGHD RMARFSPLMEENMLLASGAGSTENLYVAAAYFSSLATAGAMDLMGSYVARYGADAPPLN AMAESCYEGLVALEAIFQRAHSPEIPDLMASAHDVGFDGPRGPMCMRDSQFDQQVYIAS ADGYDFDILDTLTTLDA" gene complement(4046..5119) /gene="amiC" /note="amiC" misc_binding 4275..5225 /label="sequence-verified (primer AP340)" /note="sequence-verified" CDS 5220..5648 /codon_start=1 /transl_table=11 /gene="amiA" /product="acetamidase regulator" /label="amiA" /note="amiA" /note="marR family of regulators; AmiA" /db_xref="GI:31339873" /protein_id="DAA01132.1" /translation="MAAGQQRRPNLLLPLVRLTHLAESAIERVLADSSLKIEDWRVLDE LAGRRTVPMSDLAQATLITGPTLTRTVDRLVSQGIIYRTADLHDRRRVLVALTPRGRTL RNRLVDAVAEAECAAFESCGLDVDQLRELVDTTSNLTS" gene 5220..5648 /gene="amiA" /note="amiA" CDS 5728..6012 /codon_start=1 /transl_table=11 /gene="amiD" /product="acetamidase regulator" /label="amiD" /note="amiD" /note="AmiD" /db_xref="GI:31339874" /protein_id="DAA01133.1" /translation="MPTYTFRCSHCGPFDLTCAISERDAAATCPECRTPARRVFGSVGL TTFTAGHHRAFDAASASAESPTVVKSIPAGADRPRAPRRNPGLPSLPRY" gene 5728..6012 /gene="amiD" /note="amiD" CDS 6017..6657 /codon_start=1 /transl_table=11 /gene="amiS" /product="acetamidase transporter" /label="amiS" /note="amiS" /note="AmiS" /db_xref="GI:31339875" /protein_id="DAA01134.1" /translation="MGGVGLFYVGAVLIIDGLMLLGRISPRGATPLNFFVGGLQVVTPT VLILQSGGDAAVIFAASGLYLFGFTYLWVAINNVTDWDGEGLGWFSLFVAIAALGYSWH AFTAEADPAFGVIWLLWAVLWFMLFLLLGLGHDALGPAVGFVAVAEGVITAAVPAFLIV SGNWETGPLPAAVIAVIGFAAVVLAYPIGRRLAARQSPTLHRPRSRPPPD" gene 6017..6657 /gene="amiS" /note="amiS" misc_binding 6597..782 /label="sequence-verified (primer AP328)" /note="sequence-verified" misc_feature 6675..6677 /label="start codon" /note="start codon" CDS 6681..6698 /codon_start=1 /label="His6" /note="His6" /translation="HHHHHH" misc_binding 6711..6731 /label="TEV" /note="TEV" /note="protease site" misc_feature 6733..6781 /label="MCS" /note="MCS" ORIGIN 1 gctagagggg gcgtcaggcg ccgggggcgg tgtccggcgg cccccagagg aactgcgcca 61 gttcctccgg atcggtgaag ccggagagat ccagcggggt ctcctcgaac acctcgaagt 121 cgtgcaggaa ggtgaaggcg agcagttcgc gggcgaagtc ctcggtccgc ttccactgcg 181 ccccgtcgag cagcgcggcc aggatctcgc ggtcgccccg gaaggcgttg agatgcagtt 241 gcaccaggct gtagcgggag tctcccgcat agacgtcggt gaagtcgacg atcccggtga 301 cctcggtcgc ggccaggtcc acgaagatgt tggtcccgtg caggtcgccg tggacgaacc 361 ggggttcgcg gccggccagc agcgtgtcca cgtccggcag ccagtcctcc aggcggtcca 421 gcagccgggg cgagaggtag ccccacccgc ggtggtcctc gacggtcgcc gcgcggcgtt 481 cccgcagcag ttccgggaag acctcggaat ggggggtgag cacggtgttc ccggtcagcg 541 gcaccctgtg cagccggccg agcacccggc cgagttcgcg ggccagggcg agcagcgcgt 601 tccggtcggt cgtgccgtcc atcgcggacc gccaggtggt gccggtcatc cggctcatca 661 ccaggtaggg ccacggccag gctccggtgc cgggccgcag ctcgccgcgg ccgaggaggc 721 ggggcaccgg caccggggcg tccgccagga ccgcgtacgc ctccgactcc gacgcgaggc 781 tctccggacc gcaccagtgc tcgccgaaca gcttgatcac cgggtcgggc tcgccgacca 841 gtacggggtt ggtgctctcg ccgggcaccc gcagcaccgg cggcaccggc agcccgagct 901 cctccagggc tcggcgggcc agcggctccc agaattcctg gtcgttccgc aggctcgcgt 961 aggaatcatc cgaatcaata cggtcgagaa gtaacaggga ttcttgtgtc acnnnntcag 1021 aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcggct 1081 ttgttgaata aatcgaactt ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa 1141 cgcagaccgt tccgtggcaa agcaaaagtt caaaatcacc aactggtcca cctacaacaa 1201 agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcctg 1261 gtatgagtca gcaacacctt cttcacgagg cagacctcac tagttccact gagcgtcaga 1321 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 1381 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 1441 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct 1501 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 1561 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 1621 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 1681 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca 1741 ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 1801 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 1861 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 1921 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 1981 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 2041 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca acgcgtgcgg 2101 ccgcacgcgt gagcccacca gctccgtaag ttcgggcgct gtgtggctcg tacccgcgca 2161 ttcaggcggc agggggtcta acgggtctaa ggcggcgtgt acggccgcca cagcggctct 2221 cagcggcccg gaaacgtcct cgaaacgacg catgtgttcc tcctggttgg tacaggtggt 2281 tgggggtgct cggctgtcgc tggtgttcca ccaccagggc tcgacgggag agcgggggag 2341 tgtgcagttg tggggtggcc cctcagcgaa atatctgact tggagctcgt gtcggaccat 2401 acaccggtga ttaatcgtgg tctactacca agcgtgagcc acgtcgccga cgaatttgag 2461 cagctctggc tgccgtactg gccgctggca agcgacgatc tgctcgaggg gatctaccgc 2521 caaagccgcg cgtcggccct aggccgccgg tacatcgagg cgaacccaac agcgctggca 2581 aacctgctgg tcgtggacgt agaccatcca gacgcagcgc tccgagcgct cagcgcccgg 2641 gggtcccatc cgctgcccaa cgcgatcgtg ggcaatcgcg ccaacggcca cgcacacgca 2701 gtgtgggcac tcaacgcccc tgttccacgc accgaatacg cgcggcgtaa gccgctcgca 2761 tacatggcgg cgtgcgccga aggccttcgg cgcgccgtcg atggcgaccg cagttactca 2821 ggcctcatga ccaaaaaccc cggccacatc gcctgggaaa cggaatggct ccactcagat 2881 ctctacacac tcagccacat cgaggccgag ctcggcgcga acatgccacc gccgcgctgg 2941 cgtcagcaga ccacgtacaa agcggctccg acgccgctag ggcggaattg cgcactgttc 3001 gattccgtca ggttgtgggc ctatcgtccc gccctcatgc ggatctacct gccgacccgg 3061 aacgtggacg gactcggccg cgcgatctat gccgagtgcc acgcgcgaaa cgccgaattt 3121 ccgtgcaacg acgtgtgtcc cggaccgcta ccggacagcg aggtccgcgc catcgccaac 3181 agcatttggc gttggatcac aaccaagtcg cgcatttggg cggacgggat cgtggtctac 3241 gaggccacac tcagtgcgcg ccagtcggcc atctcgcgga agggcgcagc agcgcgcacg 3301 gcggcgagca cagttgcgcg gcgcgcaaag tccgcgtcag ccatgcatgg aggcattgct 3361 atgagcgacg gctacagcga cggctacaac cggcagccga ctgtccgcaa aaagcggcgc 3421 gtgaccgccg ccgaaggcgc tcgaatcacc ggactatccg aacgccacgt cgtccggctc 3481 gtggcgcagg aacgcagcga gtggctcgcc gagcaggctg cacgccgcga acgcatccgc 3541 gcctatcacg acgacgaggg ccactcttgg ccgcaaacgg ccaaacattt cgggctgcat 3601 ctggacaccg ttaagcgact cggctatcgg gcgaggaaag agcgtgcggc agaacaggaa 3661 gcggctcaaa aggcccacaa cgaagccgac aatccaccgc tgttctaacg caattgggga 3721 gcgggtgtcg cgggggttcc gtggggggtt ccgttgcaac gggtcggaca ggtaaaagtc 3781 ctggtagacg ctagttttct ggtttgggcc atgcctgtct cgttgcgtgt ttcgttgcgc 3841 ccgttttgaa taccagccag acgagacggg gttctacgaa tcttggtcga taccaagcca 3901 tttccgctga atatcgggga gctcaccgcc agaatcggtg gttgtggtga tgtacgtggc 3961 gaactccgtt gtagtgcctg tggtggcatc cgtggcgcgg ccgcggtacc agatctttaa 4021 atctagataa agaagtgacg cggtctcaag cgtcgagcgt cgtcagcgtg tcgaggatgt 4081 cgaagtcgta gccgtcggcg ctggcgatgt agacctgctg gtcgaattga ctgtcgcgca 4141 tacacatcgg gccccggggc ccgtcgaacc cgacatcgtg cgcggatgcc atcaggtccg 4201 gtatctcggg ggagtgggcc cgctggaaga tggcctcgag cgcaacgaga ccctcgtaac 4261 aggattcggc catcgcgttg agcggtggcg cgtcggcgcc gtagcgggcg acgtagctgc 4321 ccatcaggtc catcgcaccc gcggtggcca gtgaactgaa gtacgccgcg gcgacataga 4381 ggttttcggt ggagccggcg ccgctggcca gcagcatgtt ctcctccatc agcgggctga 4441 accgcgccat gcggtcgtgc ccgccggcgc gcgcgaactc gcggttgaac aacacggcgt 4501 cctggccgac gagcagcatc aacacggcct gcgcccccga cgcgatggcc ttgcggacag 4561 gtgcgcggaa atcgtcggtg ccgtacggga cgtagatctc ccgtctgagc tcgaggtcca 4621 gatctcggca gtacgcgcgg gcggccgcgg cggaacggcg cggccagatg tagtcatcgc 4681 cgaccaggca ccaggaccgg atgccgaagt ggtcgcgcag ccaggcgagc gcgggcgcga 4741 tctggatctg cggtgtctcg cctgtgcaga acacgcccgg tgtgcgttca ccgccctcgt 4801 acaacgaggt gtagacgtac gggatgcggt cgcggaccac cggggagatg cggttgcgca 4861 cggccgagat gtgccagccg gtcacggcgt cgagaccgtg acctcgcaac cggtcggcga 4921 cggtccgggc gacgtcgtcg ccgggcgctc cgccgtcgag cacctcgatg gtgaccttgc 4981 ggccctgcag gccgcctcgg tcgttgacct ccttggccgc gagctcggcc acggcctcgc 5041 acgaaggcgc gaagattccc gctggccctt gaagcggaat caccagcccg acgcggaact 5101 caacctcgcc gtcctgcact ccagatcacc gtcgatcccg tgtagtctgc gcttcaaagg 5161 tttctagcag aaataattca ttctgaacag accccgccgt cgacacgagg agacccaaca 5221 tggccgccgg acagcagcgc cgccccaacc tcctgctgcc gttggtgcgt ctgacccacc 5281 tcgcggagtc ggcgatcgaa cgcgtgctcg cggactcgtc gctcaagatc gaggactggc 5341 gcgtgctcga cgagttggcc ggacggcgca ccgtgcccat gagcgatctc gcgcaggcca 5401 cgctgatcac gggtccgact ctcaccagaa ccgtcgatcg ccttgtgtcg caagggatca 5461 tctaccggac tgccgatctg catgaccgcc ggcgggtgct cgtggcgttg accccgcggg 5521 ggcggacgct gcgcaaccgc ctggtggacg cggtagccga ggccgagtgt gcggcttttg 5581 aatcgtgcgg gctggacgtc gaccagttgc gcgaactcgt cgacaccacc tcgaatttga 5641 cttcgtaacc acccgcgccc ggccggcgtt cacccttgac ttttattttc atctggatat 5701 atttcgggtg aatggaaagg ggtgaccatg ccgacctaca cattccgttg ttcccactgc 5761 ggtcccttcg atctcacctg cgcgatctcc gagcgcgatg cggcggcgac ctgtccggag 5821 tgccggacgc cggcgcgccg ggtcttcggt tcggtagggc tgacgacatt caccgcggga 5881 catcaccgcg cattcgacgc ggcgtccgcg agcgccgaaa gtcccacggt ggtgaagtcg 5941 attcccgcag gcgcggaccg cccgcgggcc ccgcgccgca atcccggtct accgagtctg 6001 ccgaggtact agcgacatgg gtggcgtcgg gctcttctac gtgggtgcgg tgctcatcat 6061 cgacgggctg atgctgctgg gccgcatcag cccacgaggc gcaacaccgc tgaacttctt 6121 cgtcggcgga ctgcaggtgg tgacgcctac ggtgctgatc ctgcagtccg gcggagacgc 6181 ggccgtgatc ttcgcggcct ccgggctcta cctgttcggc ttcacctacc tgtgggtggc 6241 catcaacaac gtgaccgact gggacggaga aggtctcgga tggttctcgc tgttcgtcgc 6301 gatcgccgca ctcggctact cgtggcacgc gttcaccgcc gaggccgacc cggcgttcgg 6361 ggtgatctgg ctgctgtggg cagtgctgtg gttcatgctg ttcctgctgc tcggcctggg 6421 gcacgacgca ctggggcccg ccgtcgggtt cgtcgcggtg gccgaaggcg tgatcaccgc 6481 cgccgtgccg gccttcctga tcgtgtcggg caactgggaa accggcccgc tccccgccgc 6541 ggtcatcgcc gtgatcggtt ttgccgcagt tgttctcgca taccccatcg ggcgccgtct 6601 cgcagcgcgt cagtcaccaa ccctccaccg gccgcgctcg cggccaccac ccgataagag 6661 aaagggagtc caccatgaag caccaccacc accaccaccc gtcggccggc gagaacctgt 6721 acttccaggg cgccatggac gataaggtac cggatccgaa ttcgatatcg ctagcaagct 6781 tatcgatgtc gacgtagtta actagcgtac gatcgactgc caggcatcaa ataaaacgaa 6841 aggctcagtc gaaagactgg gcctttcgtt ttatgccatc atggccgcgg tgatca //