LOCUS       pRE107 Reconstructed.seq      6314 bp    DNA                       Thu, Feb 14, 2013
^-^-
FEATURES             Location/Qualifiers
     misc_feature    complement(2638..3498)
                     /note="bla"
     misc_feature    complement(405..833)
                     /note="TraK"
     misc_feature    56..427
                     /note="R6K-OriV"
     misc_feature    441..2163
                     /note="OriT"
     CDS             complement(4419..5840)
                     /note="sacB1"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGIS
                     HITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHG
                     YHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQT
                     QEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIF
                     DGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEES
                     LFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIAS
                     NTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLN
                     KTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLN
                     IKGKKTSVVKDSILEQGQLTVNK"
     misc_feature    complement(4304..5193)
                     /note="Pr96 seq"
     misc_feature    4419..4445
                     /note="sacB-R (Curtiss 08)"
     misc_feature    complement(4520..4543)
                     /note="PrMK097"
     misc_feature    4296..4305
                     /note="PrMK066"
     misc_feature    4418..4435
                     /note="PrMK111"
     misc_feature    complement(5209..5232)
                     /note="PrMK096"
     misc_feature    complement(5962..5989)
                     /note="sacB-F (Curtis   08)"
     misc_feature    complement(6305..6309)
                     /note="PrMK066"
     misc_feature    6305..6305
                     /dnas_title=Pr97seq1
                     /note="Pr97seq1"
ORIGIN
         1 gatcgctagt ttgttttgac tccatccatt agggcttcta aaacgccttc taaggccatg
        61 tcagccgtta agtgttcctg tgtcactgaa aattgctttg agaggctcta agggcttctc
       121 agtgcgttac atccctggct tgttgtccac aaccgttaaa ccttaaaagc tttaaaagcc
       181 ttatatattc ttttttttct tataaaactt aaaaccttag aggctattta agttgctgat
       241 ttatattaat tttattgttc aaacatgaga gcttagtacg tgaaacatga gagcttagta
       301 cgttagccat gagagcttag tacgttagcc atgagggttt agttcgttaa acatgagagc
       361 ttagtacgtt aaacatgaga gcttagtacg tgaaacatga gagcttagta cgtactatca
       421 acaggttgaa ctgctggatc ctttttgtcc ggtgttgggt tgaaggtgaa gccggtcggg
       481 gccgcagcgg gggccggctt ttcagccttg cccccctgct tcggccgccg tggctccggc
       541 gtcttgggtg ccggcgcggg ttccgcagcc ttggcctgcg gtgcgggcac atcggcgggc
       601 ttggccttga tgtgccgcct ggcgtgcgag cggaacgtct cgtaggagaa cttgaccttc
       661 cccgtttccc gcatgtgctc ccaaatggtg acgagcgcat agccggacgc taacgccgcc
       721 tcgacatccg ccctcaccgc caggaacgca accgcagcct catcacgccg gcgcttcttg
       781 gccgcgcggg attcaaccca ctcggccagc tcgtcggtgt agctctttgg catcgtctct
       841 cgcctgtccc ctcagttcag taatttcctg catttgcctg tttccagtcg gtagatattc
       901 cacaaaacag cagggaagca gcgcttttcc gctgcataac cctgcttcgg ggtcattata
       961 gcgatttttt cggtatatcc atcctttttc gcacgatata caggattttg ccaaagggtt
      1021 cgtgtagact ttccttggtg tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc
      1081 ccacccgcga gcgggtgttc cttcttcact gtcccttatt cgcacctggc ggtgctcaac
      1141 gggaatcctg ctctgcgagg ctggccggct accgccggcg taacagatga gggcaagcgg
      1201 atggctgatg aaaccaagcc aaccaggaag ggcagcccac ctatcaaggt gtactgcctt
      1261 ccagacgaac gaagagcgat tgaggaaaag gcggcggcgg ccggcatgag cctgtcggcc
      1321 tacctgctgg ccgtcggcca gggctacaaa atcacgggcg tcgtggacta tgagcacgtc
      1381 cgcgagctgg cccgcatcaa tggcgacctg ggccgcctgg gcggcctgct gaaactctgg
      1441 ctcaccgacg acccgcgcac ggcgcggttc ggtgatgcca cgatcctcgc cctgctggcg
      1501 aagatcgaag agaagcagga cgagcttggc aaggtcatga tgggcgtggt ccgcccgagg
      1561 gcagagccat gactttttta gccgctaaaa cggccggggg gtgcgcgtga ttgccaagca
      1621 cgtccccatg cgctccatca agaagagcga cttcgcggag ctggtgaagt acatcaccga
      1681 cgagcaaggc aagaccgagc gcctgggtca cgtgcgcgtc acgaactgcg aggcaaacac
      1741 cctgcccgct gtcatggccg aggtgatggc gacccagcac ggcaacaccc gttccgaggc
      1801 cgacaagacc tatcacctgc tggttagctt ccgcgcggga gagaagcccg acgcggagac
      1861 gttgcgcgcg attgaggacc gcatctgcgc tgggcttggc ttcgccgagc atcagcgcgt
      1921 cagtgccgtg catcacgaca ccgacaacct gcacatccat atcgccatca acaagattca
      1981 cccgacccga aacaccatcc atgagccgta tcgggcctac cgcgccctcg ctgacctctg
      2041 cgcgacgctc gaacgggact acgggcttga gcgtgacaat cacgaaacgc ggcagcgcgt
      2101 ttccgagaac cgcgcgaacg acatggagcg gcacgcgggc gtggaaagcc tggtcggctg
      2161 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
      2221 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag
      2281 cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat
      2341 ctccttgctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
      2401 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
      2461 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
      2521 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccaaaa
      2581 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
      2641 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
      2701 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
      2761 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
      2821 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
      2881 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
      2941 tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
      3001 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
      3061 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
      3121 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
      3181 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
      3241 ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat
      3301 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
      3361 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
      3421 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
      3481 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
      3541 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
      3601 gcgcacattt ccccgaaaag tgccacctgc agatctgcag gtcgacggat cccaagcttc
      3661 ttctagaggt accgcatgcg atatcgagct ctcccgggaa ttCATGCAGT TCACTTACAC
      3721 CGCTTCTCAA CCCGGTACGC ACCAGAAAAT CATTGATATG GCCATGAATG GCGTTGGATG
      3781 CCGGGCAACA GCCCGCATTA TGGGCGTTGG CCTCAACACG ATTTTACGTC ACTTAAAAAA
      3841 CTCAGGCCGC AGTCGGTAAC CTCGCGCATA CAGCCGGGCA GTGACGTCAT CGTCTGCGCG
      3901 GAAATGGACG AACAGTGGGG CTATGTCGGG GCTAAATCGC GCCAGCGCTG GCTgTTTTAC
      3961 GCGTATGACA gTCTCCGGAA GACGGTTGTT GCGCACGTAT TCGGTGAACG CACTATGGCG
      4021 ACGCTGGGGC GTcTTATGAG CcTGCTGTCA CCCTtTGACG TGGTGATATG GATGACGGAT
      4081 GgCTGGCCgC TGTATGAATC CCGCcTGAAG GGAAAGCTGC ACGTAATCAG CAAGCGATAT
      4141 ACGCAGCGAA TTGAGCGGCA TAACCTGAAT CTGAGGCAGC ACCTGGCACG GCTGGGACGG
      4201 AAGTCGCTGT CGTTCTCAAA ATCGGTGGAG CTGCATGACA AAGTCATCGG GCATTATCTG
      4261 AACATAAAAC ACTATCAATA AGTTGGAGTC ATTAcCAAAA GGTTAGGAAT ACGGTTAGCC
      4321 ATTTGCCTGC TTTTATATAG TTCATATGGG ATTCACCTTT ATGTTGATAA GAAATAAAAG
      4381 AAAATGCCAA TAGGATATCG GCATTTTCTT TTGCGTTTTT ATTTGTTAAC TGTTAATTGT
      4441 CCTTGTTCAA GGATGCTGTC TTTGACAACA GATGTTTTCT TGCCTTTGAT GTTCAGCAGG
      4501 AAGCTgGGCG CAAACGTTGA TTGTTTGTCT GCGTAGAATC CTCTGTTTGT CATATAGCTT
      4561 GTAATCACGA CATTGTTTCC TTTCGCTTGA GGTACAGCGA AGTGTGAGTA AGTAAAGGTT
      4621 ACATCGTTAG GATCAAGATC CATTTTTAAC ACAAGGCCAG TTTTGTTCAG CGGCTTGTAT
      4681 GGGCCAGTTA AAGAATTAGA AACATAACCA AGCATGTAAA TATCGTTAGA CGTAATGCCG
      4741 TCAATCGTCA TTTTTGATCC GCGGGAGTCA GTGAACAGaT ACCATTTGCC GTTCATTTTA
      4801 AAGACGTTCG CGCGTTCAAT TTCATCTGTT ACTGTGTTAG ATGCAATCAG CGGTTTCATC
      4861 ACTTTTTTCA GTGTGTAATC ATCGTTTAGC TCAATCATAC CGAGAGCGCC GTTTGCTAAC
      4921 TCAGCCGTGC GTTTTTTATC GCTTTGCAGA AGTTTTTGAC TTTCTTGACG GAAGAATGAT
      4981 GTGCTTTTGC CATAGTATGC TTTGTTAAAT AAAGATTCTT CGCCTTGGTA GCCATCTTCA
      5041 GTTCCAGTGT TTGCTTCAAA TACTAAGTAT TTGTGGCCTT TATCTTCTAC GTAGTGAGGA
      5101 TCTCTCAGCG TATGGTTGTC GCCTGAGCTG TAGTTGCCTT CATCGATGAA CTGCTGTACA
      5161 TTTTGATACG TTTTTCCGTC ACCGTCAAAG ATTGATTTAT AATCCTCTAC ACCGTTGATG
      5221 TTCAAAGAGC TGTCTGATGC TGATACGTTA ACTTGTGCAG TTGTCAGTGT TTGTTTGCCG
      5281 TAATGTTTAC CGGAGAAATC AGTGTAGAAT AAACGGATTT TTCCGTCAGA TGTAAATGTG
      5341 GCTGAACCTG ACCATTCTTG TGTTTGGTCT TTTAGGATAG AATCATTTGC ATCGAATTTG
      5401 TCGCTGTCTT TAAAGACGCG GCCAGCGTTT TTCCAGCTGT CAATAGAAGT TTCGCCGACT
      5461 TTTTGATAGA ACATGTAAAT CGATGTGTCA TCCGCATTTT TAGGATCTCC GGCTAATGCA
      5521 AAGACGATGT GGTAGCCGTG ATAGTTTGCG ACAGTGCCGT CAGCGTTTTG TAATGGCCAG
      5581 CTGTCCCAAA CGTCCAGGCC TTTTGCAGAA GAGATATTTT TAATTGTGGA CGAATCGAAc
      5641 TCAGGAACTT GATATTTTTC ATTTTTTTGC TGTTCAGGGA TTTGCAGCAT ATCATGGCGT
      5701 GTAATATGGG AAATGCCGTA TGTTTCCTTA TATGGCTTTT GGTTCGTTTC TTTCGCAAAC
      5761 GCTTGAGTTG CGCCTCCTGC CAGCAGTGCG GTAGTAAAGG TTAATACTGT TGCTTGTTTT
      5821 GCAAACTTTT TGATGTTCAT CGTTCATGTC TCCTTTTTTA TGTACTGTGT TAGCGGTCTG
      5881 CTTCTTCCAG CCCTCCTGTT TGAAGATGGC AAGTTAGTTA CGCACAATAA AAAAAGACCT
      5941 AAAATATGTA AGGGGTGACG CCAAAGTATA CACTTTGCCC TTTACACATT TTAGGTCTTG
      6001 CCTGCTTTAT CAGTAACAAA CCCGCGCGAT TTACTTTTCG ACCTCATTCT ATTAGACTCT
      6061 CGTTTGGATT GCAACTGGTC TATTTTCCTC TTTTGTTTGA TAGAAAATCA TAAAAGGATT
      6121 TGCAGACTAC GGGCCTAAAG AACTAAAAAA TCTATCTGTT TCTTTTCATT CTCTGTATTT
      6181 TTTATAGTTT CTGTTGCATG GGCATAAAGT TGCCTTTTTA ATCACAATTC AGAAAATATC
      6241 ATAATATCTC ATTTCACTAA ATAATAGTGA ACGGCAGGTA TATGTGATGG GTTAAAAAGG
      6301 ATCaGAATTa attc
//
