LOCUS       Ple205\(pEMS1231       18751 bp    DNA     circular      3-FEB-2010
SOURCE      
  ORGANISM  
COMMENT     PCR genotyping:
            Sense primer: oEMS2364 - 5' GCGTATCACGAGGCCCTTTC - 3' Tm=56.0 Deg C
            Antisense primer: oEMS3201 - 5'  GAATCCACATTCCTGCCCCTC - 3 Tm=57.0 Deg C
            
            Cycling conditions:
            Step 1: 94 Deg C for 3 min
            Step 2: 94 Deg C for 1 min
            Step 3: 61 Deg C for 1 min
            Step 4: 72 Deg C for 45 sec
            Step 5: repeat Step 2-4 34 times
            Step 6: 72 Deg C for 5 min
            
            244 bp product
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|543242102|
COMMENT     VNTDBDATE|544984798|
COMMENT     LSOWNER|
COMMENT     VNTNAME|Ple205 (pEMS1231)|
COMMENT     VNTAUTHORNAME|Russell Bonaguro|
COMMENT     VNTOAUTHORNAME|Demo User|
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COMMENT          #138=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #139=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #140=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
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COMMENT                            1.74233 0.833333 0 "Ple205 (pEMS1231)" "@N" 1 0 
COMMENT                            0.871165 0 -10 11.4392 1.47361 Nil) 
COMMENT                      #156=(CLabel 
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COMMENT               #157=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -1162 100) 
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COMMENT                                   -10 7.99245 1.17389 #159#) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                   13.0756 1.74233 0 -10 2.67247 1.17389 #161#) 
COMMENT                             #163=(CScratch 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
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COMMENT                                   -10 10.0405 1.17389 #163#) 
COMMENT                             #165=(CScratch 
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COMMENT                                   "SV40 early polyA signal" "@N" 1 16.1543 
COMMENT                                   -5.22699 0 -10 11.0645 1.17389 #167#) 
COMMENT                             #169=(CScratch 
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COMMENT                                   "FFRT (F5 mutant)" "@N" 1 14.5809 5.22699 0 
COMMENT                                   -10 7.99245 1.17389 #169#) 
COMMENT                             #171=(CScratch 
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COMMENT                                   "Ple205 (SLC7A5-C)" "@N" 1 10.3589 10.454 0 
COMMENT                                   -10 8.59188 1.17389 #175#) 
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COMMENT                                    (CWidget 0 (3 #16# 0) 1 2 0 0 #117# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.670712 5.46926 
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COMMENT                             #178=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                   "@N" 1 12.793 6.96932 0 -10 6.16917 1.17389 
COMMENT                                   #177#) 
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COMMENT                                   "Region 4 (reverse complement)" "@N" 1 
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COMMENT                             #187=(CLabel 
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COMMENT                                   -10.7272 -8.71165 0 -10 2.87229 1.17389 #186#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #188=(CGroupWidget 
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COMMENT                            (CObjectList 
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COMMENT                                   1 4.25685 13.9386 0 -10 4.79547 1.17389 #189#) 
COMMENT                             #191=(CScratch 
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COMMENT                                   (LOGPEN 0 8 13395507) 10 1 6.25545 1.9 
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COMMENT                            (CObjectList)) 
COMMENT                      #193=(CGroupWidget 
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COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "5' homology sequence" "@N" 1 -16.4471 
COMMENT                                   1.74233 0 -10 10.4901 1.17389 #194#) 
COMMENT                             #196=(CWideArrow 
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COMMENT                                    (CWidget 0 (3 #3# 0) 1 2 0 0 #117# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                             #197=(CLabel (CWidget 0 (0 0) 1 2 0 0 #113# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "3' homology sequence" "@N" 1 5.4146 -12.1963 
COMMENT                                   0 -10 10.4901 1.17389 #196#) 
COMMENT                             #198=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #4# 0) 1 2 0 0 #117# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                                    5.07984 1 0.082322) 0.8 1.8 0) 
COMMENT                             #199=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "HPRT complementary sequence" "@N" 1 18.3144 
COMMENT                                   -6.96932 0 -10 14.9609 1.17389 #198#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #200=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -185 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #201=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -255 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #202=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -395 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) 
COMMENT        (CObList #203=(AnlzFontColorItem 276 296 41943168) 
COMMENT         #204=(AnlzFontColorItem 53 72 41943168)) 1 
COMMENT        (CObList #205=(AnlzFontBackColorItem 1991 2402 33554687) 
COMMENT         #206=(AnlzFontBackColorItem 138 1310 33619967) 
COMMENT         #207=(AnlzFontBackColorItem 1311 1990 50331392))) (CObList) 0 
COMMENT       (CStringList) 0 1684625219 (CObList)))
FEATURES             Location/Qualifiers
     misc_recomb     12614..16425
                     /vntifkey="86"
                     /label=5'\homology\sequence
     misc_marker     complement(17524..18745)
                     /vntifkey="22"
                     /label=AmpR
     misc_marker     11163..12588
                     /vntifkey="22"
                     /label=AmpR
     misc_recomb     7522..11167
                     /vntifkey="86"
                     /label=3'\homology\sequence
                     /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3"
     misc_recomb     3568..7521
                     /vntifkey="86"
                     /label=HPRT\complementary\sequence
                     /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2"
     misc_feature    2470..2480
                     /vntifkey="21"
                     /label=Kozak\consensus
     misc_feature    2477..3196
                     /vntifkey="21"
                     /label=EGFP
     misc_feature    3197..3232
                     /vntifkey="21"
                     /label=Tetracysteine\residue
     misc_feature    3233..3305
                     /vntifkey="21"
                     /label=NLS
     misc_feature    3512..3562
                     /vntifkey="21"
                     /label=SV40\early\polyA\signal
     misc_feature    2422..2469
                     /vntifkey="21"
                     /label=FFRT\(F5\mutant)
     misc_feature    3311..3358
                     /vntifkey="21"
                     /label=FFRT
     misc_feature    12595..12612
                     /vntifkey="21"
                     /label=I-Sce-I
     misc_feature    139..2399
                     /vntifkey="21"
                     /label=Ple205\(SLC7A5-C)
                     /note="1307"
     primer          277..297
                     /vntifkey="27"
                     /label=oEMS3201
     primer          54..73
                     /vntifkey="27"
                     /label=oEMS2364
     misc_feature    1992..2403
                     /vntifkey="21"
                     /label=Prom\Region
     misc_feature    139..1311
                     /vntifkey="21"
                     /label=Region\3\(reverse\complement)
     misc_feature    1312..1991
                     /vntifkey="21"
                     /label=Region\4\(reverse\complement)
BASE COUNT     4905 a      4287 c      4422 g      5137 t 
ORIGIN
        1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 
       61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 
      121 cgagctccta ggccggccca ggtgttgagc aggtggtatg gggcgggcgg ggaagggtgc 
      181 cctcaggtcc atcagaacag ccacaagagc cctggggtgg gagggggccg atattctggt 
      241 cggcacagag tgggctgcca ggagagggcg ggggcggagg ggcaggaatg tggattctgt 
      301 cccaaggggg caaggctgag gggatgtagc tgggcacatg gtctgctctg aattgagctt 
      361 ggttctctag agggtcgatg ggaaggacgt gctggggatg gggaggaagc ggaggaggag 
      421 gaggcggtgg cagctgggaa caggcaatgc ttctcacaca ggcgtatctt tattaaacaa 
      481 gaaaataaga taaaagcaaa gggaggaagc cttgaacctt aaaagctggg ccagaaattc 
      541 tacaatacaa tcagaagcca caatctggtc acagtgagag cccttcccgc agagccgcag 
      601 acaccggccg ctgcccctgg ggcgggaggg aagcctcctg tttgtttttt gtcccgttgc 
      661 tttgagggct ggaatcaatg tcttttcctc tcggcgtgtt tgtggtttca tctcagtgtg 
      721 tccaggctcg gatggcttcc cgggagtatc tccgtgtctc aggattagca ccagttttta 
      781 aaaataactg acaaaggcca tttttcagga gacaaagatg ttggtgggtg cagagggccc 
      841 cctgtgtgtt gatacggaaa atgaaggtca gccactccct gcttctcttg gtgtcttgtt 
      901 ggacacgatg tcaacatggg gccggtagaa ctggagccca gggcccctct ggccgcttgg 
      961 gcctttctct gtccccaagc ccacctggaa atgggcagcc catggagaat ggctgaatag 
     1021 ctgctctctg cccccatccc aagccccagg ttcagagacc accccctcca ttgactgtgt 
     1081 caccccttcc ccagcagagg agcgggctgt tctcagagcg ctgcaggaag cccagacaag 
     1141 tgccttcctc tggctgctgc ttccatcagc gtgaactgcc cggcattgta ggataagttt 
     1201 tctcctgggt tttaaggttc aggactttct ctctttgctg cgctttttcc ttgactgatt 
     1261 gcacaaatca gagaaggcaa gagtgaaatg ctgcgtatcc cttggcctag cggtgaagaa 
     1321 cacaccagca ccatgcccac caaaacagga gccccatggc tgtttcccag agaaacctgg 
     1381 gacccacacg tccattcact gcaacccaga ctgaatctgc agccaagctc cgctctctca 
     1441 ctactgggaa ccctcagggg ggatccctta gcctaaacca gcaccagtca ctacccgcac 
     1501 acgattttat tactactgtt ttcttatgac tcagcccgtg tcactgattc ctgacgagct 
     1561 ggtgtggaga gcagaaatga atgatttttt ttttaactgc aatttccttc tcaccaacca 
     1621 gtcagcaatc ggagctctaa cagtaaaccg attgctcagt tactccacgt gggtgccacg 
     1681 caagaggctg ggagtattgc tcagcggggc caacgggagg ctggcacttg cctgaaccgc 
     1741 ttaagtgggg gaatggaatg cccaccgaag ggcagggcag ggaccctgct gtcccgtcac 
     1801 tgaagatatt cccagcctgt ggggagaagt gtaaaagaat gaatgaaaga aacgcacgtg 
     1861 gtttctggga ccaatgacct tagcctagaa ctgccgggat ggtccaggaa cgtaaagatg 
     1921 tagagatgtg ggagccaggg gcgtagtgat gcaaggatgc agggacccag agactacgac 
     1981 ctcccaaccc ctcccaggct tgacgagagg ctgcgggcca gtgggtgaag gggcgcgccc 
     2041 tgactgccag gccccgccca ggcgcatctg ggaggacggg ctgggatgac gcgggcgccg 
     2101 ggagggggga ggtcgcgagg ccggggtctc catgaagcag gaggactggg gccttcgagg 
     2161 accacgcggg cctgggaata gcccgccagg ctgggccgga cgacgcacgt gctccgagct 
     2221 gggccgaggg ggcggggctg aggaacgggg ccgggccacg gggcggggag gagccgcaga 
     2281 cggtgggcgg ggccggcggg ccggggccta aaaggcggcg cgggcggggt tcctgacgca 
     2341 gctgcgggcg gcgggcggcg cgcacactgc tcgctgggcc gcggctcccg ggtgtcccag 
     2401 gccggccacg cgtggcgcgc cgaagttcct attccgaagt tcctattctt caaaaggtat 
     2461 aggaacttcc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat 
     2521 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga 
     2581 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc 
     2641 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta 
     2701 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca 
     2761 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt 
     2821 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg 
     2881 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc 
     2941 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg 
     3001 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct 
     3061 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa 
     3121 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga 
     3181 cgagctgtac aagttatttc tgaactgctg cccgggctgc tgcatggaac cggatccaaa 
     3241 aaagaagaga aaggtagatc caaaaaagaa gagaaaggta gatccaaaaa agaagagaaa 
     3301 ggtaatataa gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcag 
     3361 cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta 
     3421 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt 
     3481 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 
     3541 aataaagcat ttttttcact gcgaattctc gtaaaactct tcatggcagt agttattatt 
     3601 ctctctctct ctctttttct tttttcttga gacaggatat ttctctgttg cccaggctgg 
     3661 agtgcagtgg cacagtcttg gctcactgca gcctggacct cctgggctca agccatcctc 
     3721 ccacctcagc ctcccaagta gctggggcta caggcacatg gccaccaggc ccagataatt 
     3781 tttcattttt gtagagactg agtctcacca tgttacccag gtttattatt ctcatttttt 
     3841 agatgaagag actgaggtcc agagaagctc aatgacttgc ctagttttac aaatctcctg 
     3901 ccatcacata cccctcagcg tccttaataa gagggaggcc accaactatg tgctgggcac 
     3961 tgtggtggat gctggagcta tagggttgag tatataagaa atggtgttgc tggagcaact 
     4021 gttgcttgct tacctgacct atctgagaat taattagcag gggaacatat ttttgttttc 
     4081 agattcaata taagaacttg tgtgggcaaa aataaagatc agtagtaata acagtagttc 
     4141 ccatttgctg actgtactgt cctaagtgca tatatatata catacacaca cgcataccta 
     4201 tactcctcta atactcaaaa tgatcctgtt tatgtattgt taatatgctc attttatttt 
     4261 taaattttta tttattttta tttttattta tttttgagac ggagtctcat tctgtcgcgg 
     4321 aggctgaagt gcagtggtgc gatctcagct cagtgcgacc tccgcctccc gggttcaagt 
     4381 gattctcctg cctcagcctc cggactagct gggattacag gcgcccgcct ccacgcccag 
     4441 ctaatttttg tatttttagt agagatgggg tttcgccatg ttggccaggc tggtctcgta 
     4501 ctcctgacct tgagtgatcc acctgcctcg gcctcccaaa gtgctgggat tacaggcatg 
     4561 agccaccgcg ccgggctaat atgctcattt tagtgaggca aaaatagagg ctcagagtct 
     4621 gatttgtaca aaactacaga gcagttaagt gtcctctcag atgtgtaccc tgatctgggt 
     4681 gactctagga ctctaggtct caactgttac aaccagttaa gggtttgggg aagcactggg 
     4741 ccaagagtca ggaaaatgga agccacaggt agtgcaaggt cttgggaatg ggacgtctgg 
     4801 tccaaggatt cacgcgatga ctggaacccg aagagccggg gcccggttta cggccgccat 
     4861 gaagcaacgc gcgccggtag gtttgggaat cagggagccc tctgaatagg agactgagtt 
     4921 gggagggaaa ggggcttcgc tgggggagcc tcggcttctt ctgggagaaa attcccacgg 
     4981 ctacctagtg agcctgcaaa ctggtaggcg ccggcgtagg cgcgcgggcg gggccggggg 
     5041 cggggcctgc ggggcgtggc ggggcgggca gagggcgggg cctgcttctc ctcagcttca 
     5101 ggcggctgcg acgagccctc aggcgaacct ctcggctttc ccgcgcggcg ccgcctcttg 
     5161 ctgcgcctcc gcctcctcct ctgctccgcc accggcttcc tcctcctgag cagtcagccc 
     5221 gcgcgccggc cggctccgtt atggcgaccc gcagccctgg cgtcgtggtg agcagctcgg 
     5281 cctgccggcc ctggccggtt caggcccacg cggcaggtgg cggccgggcc ctgaggcgcg 
     5341 ggatcgatcc atgacacccc cccgcccccc atatctcact ataattcact gtcaaatggc 
     5401 ttttatgatt tggagtttgg ggatactttt aaagacaggg tcttggtagc tcctagatgg 
     5461 cttgggattc acttagtaac ccttgctggc ctaaaattca ggaacttcta cctcatcttc 
     5521 ccaagtactg ggttagtagg gatgtccagc atatttagct tgctttaatg attttgctgt 
     5581 acttttttct gtgtgtacag gtgcatgtat atttataaaa ctaacataat atattttacc 
     5641 cccaaagtac cataacccta attaatgcta ttaaatgttt tgtatgctgg gctctgggca 
     5701 tactaatata accaatatta tacaatgcaa aacaattttg gaatatcttc tgtaaaatta 
     5761 tcttattagt aagttgtaac catctcagac ttctaagttt gagtttgtaa aggatcagtt 
     5821 ttttttggtg acctacacac acacacacac acacacacac acacacacac acaggggggt 
     5881 ggattctgag aatttagaga atttagatgg ttaataccag tttttatttt tattctgtgt 
     5941 gatatttatt tattaagatt tatttattta ttacatgtag gtacactgta tctgtcttca 
     6001 gacacaccag aagagggcat cagatctcgt tacagatggt tgtgagtcac catgtggttg 
     6061 ctgggatttg aactcaggac ctctggaaga acagtcagtg ctcttaaccg ttgagccatc 
     6121 tcaccagccc cgtgatattt tatttaactt actagaatat actgtgttaa aacatatttt 
     6181 aggggattgg ggagatggcc cattctgtaa agtgtctgcc atgtcatctt caggacctgc 
     6241 gtttgtaacc ccagcatgga gaagacagag acagggagac ccctgggctt cctggcaagc 
     6301 tagactagca aatttggtta actaggttca atgagagact cagtctcaaa acataagaag 
     6361 tgaatttttt agacccaatt tcaatctctg aactccatac gtatgcacat gtacctgcat 
     6421 ataaatatat atacacaccc ataaacacat gcacacacaa aaaatggttc aaaagttctt 
     6481 aaaatgtata agagtagaaa gtgtagagcc gggagtggtg gcgcacgcct ttaatcccag 
     6541 cacttgggag cagaggcagg tggatttctg agttcgaggc cagcctggtc tacagagtga 
     6601 gttccaggac agccagagct atacagagaa atcctgcctc aaaaaaccaa aaataaagtg 
     6661 tatatactaa tctttcatgc ctaaaatatt tatttttgcc acatttttca tacctcttta 
     6721 atatttttct ttttctcttt gtgcttcaaa ggaaattttg tgtcatgttt tacttttcat 
     6781 tcttgactct attcagcagt aagacgcagc atggctttct tagagaagta taatctatta 
     6841 caaaacggta acatttaact gctctacata ccctcttgta gctaaattcc tctgttaaac 
     6901 taagacttat tatctaaacg taattaaatg ttgatatctt ataatgctcc actttgaaac 
     6961 agctgtcttt cttttgcaga ttagcgatga tgaaccaggt tatgacctag atttgttttg 
     7021 tatacctaat cattatgccg aggatttgga aaaagtgttt attcctcatg gactgattat 
     7081 ggacaggtta gtaagacctc gattgaagtt atttttagag ggtgtgtgtg tggtgtgtgt 
     7141 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtaagagaga gaggagaggc atgtgtgata 
     7201 tatatgtgaa gtgtattgca tgtacatgga atccagagaa ggacattagg tgtcctactc 
     7261 tgttgctctc ttattccctt gaatcagtgt ctcttgctga acctggggca tacatactga 
     7321 tttggctagg ctgcctggct agcaagaccc agggatctgc ttgtctctgc cccccagtgc 
     7381 tgacgttaca agcaggtgca accacatctg gtttttatat gggtactggg ggatctgaac 
     7441 ttagggcctg atgcgtgccc aagtgctctc acccgccaag ccacctctcc agccaaaggg 
     7501 ttttttttct tgtattaatt ctaacttagc aactgttgag tagttgatgt ttgaatataa 
     7561 gctaagccaa cttattagaa taaatgtttt ctatttttta cttgtatgtg tgagtgcctg 
     7621 catgtatgta tgtgaagcat atgtattcca ggatgtcaga attgtgatgg attccctgga 
     7681 actggagtta cagaccattg tgaaccactg tgtatgggtg ctgggaactg aactttaggt 
     7741 cctctgcaaa aacagtaagg atcttaactg ataaactatc tttctagccc taaggctttc 
     7801 tacttttaaa gttcgtttct ctttctctct ctctctctct ctctctctct ctctctccct 
     7861 ctccctccct cctcctcctc ctcctctttc ccccctctcg cctcctcttt ctctctcccc 
     7921 tccctctctc tctctcacac acacacacag acagagagag gagagagaaa ggaagcctta 
     7981 ctaattaatt acctagtttt agacataact agtgggaatc catagtaagt aaatcaacat 
     8041 tcttgaagag aggttagaag aattggctct cgttgtccat cgaggctgta taagattatt 
     8101 cttctgtttt ctcagcttac catacatatt ttctacgtag acaataccaa ttcaaataca 
     8161 ctgtacgtaa agtatatgcg cacacacaca cacacacaca cacacacaca cacacaagtt 
     8221 acatattgtc atatagtact aaaatttatg gactttttca tattaataca tctagagcta 
     8281 tcatgttttt tatcttttaa aaattacttt acggggctac agagatgtct cagcagttag 
     8341 gagcactgac tgctcttcca gaggtcctga gttcaattcc cagcaaccac atggtggctc 
     8401 acaaccatct gtaatgggat ctgatccact cttatggtgt gtctgaagac agtgacagtg 
     8461 tactcatata catgaaataa atctttaaaa aattacttta ccatcagggt atatatatat 
     8521 atatatatgt gtgtgtgtgt gtgtgtgtgt atgaagtatt tgctatttga gagggtctca 
     8581 ctgtttagct cagtctggct tggaacgttc tgtatagccc agactggcct caactcagca 
     8641 gtccccctga aaggctctca tatgtggaga ttacaggtat gtgctactgt gcccagccct 
     8701 acatgttact ttgtttttga aatagggtat caaatagctt agactggctt ctaagtcagt 
     8761 gtgtagctaa ggatggtctg agcttctgaa cctgctactt caactcctgg tgtgccatca 
     8821 tggccagttt gcacagttgt gagagtagaa ctcaggacct cctatgtgtt actagacatg 
     8881 ttctctacca gctgagctac atcccaattc gcctacattt acttttattt tttaaaagat 
     8941 ttatttatgt atacagcact ctgcctgcat gtacatctgc atgccagaag agggcaccag 
     9001 atctcatcat acatagttgt gagccaccat gtggttgctg gggattgaac tcagggcctc 
     9061 tggaagagca gtcagtgctc ttaacctctg agccatctct gtagccccct acatttattt 
     9121 taaagattat aggctattct attcatagat gtgccataac tgatttaacc agatcttcat 
     9181 tattttgtac ttagtagagt atattagagg gaatgaatct taaatacaca ttttgacact 
     9241 tgctcagctt tagacaagta aaaatcactg gtcaaggaaa tggtgcttag tggatgcttc 
     9301 tgaactgtca aattgtcctc caacaagtta catcatttta tacttccaac gttatgtgag 
     9361 agttcatttt tctttaggct cttctagtat tttatatttg agaaataaaa tttaaccaaa 
     9421 atttgccaat ctagtaagct tgtttttatt tgtatcttta aattttatga tagatgttat 
     9481 agtgtactct cctctcccct cccctcccct ctcctcctca gcaggatctt gttatgtagc 
     9541 cctaagggat ctaatactta ctctgtagtc caggctgtcc ttcagctcat gagacacacc 
     9601 atcatgcttg gcatgtttct tgttctgaaa ggtatctggt tttaattttg gttgagctgg 
     9661 tgtgttggtg tatatctgta atctcagcac aggcaagtag agacaaggat tatgattaca 
     9721 gtccagcctg ggctatttag agggattctc tttaatcttt cctcatgccc caaaatctta 
     9781 cctttggtat atgaaaaata gtctccactt ctgcaaaata ttgctttatg aagtaagaat 
     9841 taattccctt catagagaca aggaatgtgt cctgtaaaag tttaatgtgt aagaagtatt 
     9901 tgttataaaa gataaatatt cagaatcttc tttttaattc ctgattttat ttctatagga 
     9961 ctgaaagact tgctcgagat gtcatgaagg agatgggagg ccatcacatt gtggccctct 
    10021 gtgtgctcaa ggggggctat aagttctttg ctgacctgct ggattacatt aaagcactga 
    10081 atagaaatag tgatagatcc attcctatga ctgtagattt tatcagactg aagagctact 
    10141 gtgtaagtat aattaactta taattaaaaa aatagggcca ttctagtttt atctatattt 
    10201 tttttaaact tgtgcaaact atgctacata atcaacttac ttgattatgt aatatatata 
    10261 gtaatagttg gaattactat tattacatat ttatttttct gtgctggggt agaacccagg 
    10321 gaactaagta ctgtaataac cgagcttcat tcatcctagt catttttaat tttttttttt 
    10381 ttttttgttt tttcgagaca aggtttctct gtgtagccct ggctgtcctg gaactcactc 
    10441 tgtagaccag gctggcctcg aactcagaaa tctgcctgcc tctgcctccc agtgctgaga 
    10501 ttaatgacgt gcaccaccac tgcctggcat ttttttaaag ttttaatttg agaccctttc 
    10561 tcactaactt gctcacacta tcctcgaact cattacgtgg gctggtctag aaatttcagt 
    10621 caacttgcct cagcttctca agtagctggg attacaggca ggtgctacca cttctggcca 
    10681 tgatcattgt tgttgttgct attattatta ttattatttc actgatactt catatcacaa 
    10741 cacagaatag taactgtagt tcatgaccaa caaaaaagtt gtacagtttc tctgttgtct 
    10801 tattttgatc tggattttga tttgtttcat ggcactgggc aggctaatct caaactgcat 
    10861 ggctcaaata gtccttctgt cttaatctcc catgttctgg aagtataggt atcactacac 
    10921 ctagctgtat tttcatattt taaaaaaaac gcctaagaat tgctattgaa tgaggcggag 
    10981 agatggctca gcagtcacaa gtacttgtag aggactgggg tttggttact agcacctcaa 
    11041 agatggctta caattatctg tagctccaat tccagaggat ctgtgtggta aagctgcatg 
    11101 tatatgggca tgcaggtaaa actgctaaac atataaaaat agctcagtct taagagaatt 
    11161 ggtcgacttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 
    11221 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg 
    11281 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt 
    11341 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc 
    11401 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 
    11461 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 
    11521 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 
    11581 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 
    11641 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 
    11701 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac 
    11761 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 
    11821 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 
    11881 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 
    11941 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 
    12001 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 
    12061 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 
    12121 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 
    12181 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc 
    12241 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 
    12301 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 
    12361 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 
    12421 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 
    12481 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 
    12541 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccaggt cgagtaggga 
    12601 taacagggta atggatcctc gttgggcacc tgcactcatg tacgcaggcc cacatataga 
    12661 tgtacacacg catatcattc aaaatagaaa ttatttatac aagtagtgcg ttggagagct 
    12721 ggctcaggag ttgttaatgc ttgctaccct tgcaaaggac ccaagttcaa tttccagcac 
    12781 ctgtgttggg catctcacaa ccacatgtaa atctagtttc aggggatttc acccctcttc 
    12841 tgaactcttc aggcacttat acccacaaat ggacatctcc acatagagac acatactttt 
    12901 aaaaaagtaa tctagggtct agagaaatgg ttatagacca cggactggga tcagttccca 
    12961 gaagccacat cctgtggctc acaattgcct gtgaatcaag ttctagatct ggtggcctcg 
    13021 tctagcctgc agtcatcaac aaacacatgc acacatccat acacacataa tttaaaagaa 
    13081 agcgaaagta acctataatg ctaaacaaag cacattctca gtgtagactg cagtggtgat 
    13141 gaaccttgaa aatatattaa tagaagccag acacgtaagt cacaaatggt ataactcaat 
    13201 ttatatgaaa ttcccagaga gtccaaagtt aaaaaaaaca aaaaggcaga ttattggttg 
    13261 tcagtagcta cagagatggg acattgaaga tattaatagg tatagggttg aggattcatg 
    13321 gcctttccct cttccatgtt agcatgtcta ttgttgttaa ccttattcaa ctcatgttag 
    13381 ggcaatcata ttgatgagac ttaatgagta tagcttgaca ttcctagaag acagagtctc 
    13441 acatcagact ctctgatcct ctgactctta gaatctttca acaccctctt ctgaaatgat 
    13501 ctctgagcct ttggttgtga ctgaattatg agaaagaatt ttatgttgaa tgaattttac 
    13561 ttcagcattt ttacaaaact gaagctgggg atggcgtttc atgcgtgcag tccctaccat 
    13621 ttgcctataa gcctggagga ggcagaagta gaattaggct tttcagggac atcttcagct 
    13681 gcatcatgag cttgaggaca acttgagcta cataagattc ccatccccac ccccacacac 
    13741 acacacacat ctagtatgta cctagtatgc agtcttttca tatgtttaaa atgtagaaag 
    13801 gtatatgtga agtctccctt ttccccacaa cttagtccct ccaaagtatt gcatcagttt 
    13861 ccttgtacat gattttatgc atatataaga tacactattt ctctctctct ctctctcctt 
    13921 ccctccttcc ctccaacctc tgggtatgag ttgagggtta tgtatgtttc aagttgtaat 
    13981 aagaattgcc actcaaacat ttcatcccat tttaagttaa gtttgtctga ttttccactt 
    14041 tagaagggca gccatgatgg ctcatgccta taatcccatc ctagcacttg taagcctaat 
    14101 acataagact ggaggattgt catcagcttg aggctagcct ggtctaccta atgagttcca 
    14161 gccagccaag agtagaatat aagaccctgt ctccaaaaaa tgtaccaaag gggctggagg 
    14221 ggtaactcag tggttaagaa cagtagctgc tcttccagag gacctgggtt caattcccag 
    14281 cacccacatg gcagcttgca actatctgta actgtccagc tttgggatat ccgacaccat 
    14341 tataccaatt acataaaatg aaaaaattgt aaaatgtgct aaaatgagtg gggaggggga 
    14401 gcatggtgtg gttctgtggg cttgggattt tgccatgaat ccatgactat atctcagcaa 
    14461 tatgtggatt atttctggaa acctgtgggg catgggtgta tgggaagagg ggagagagag 
    14521 agagcccgca tccagccaga gttcctgtgc tctgggcagg cggacgtggg aggactgctg 
    14581 gacgatttcc acttggccct gggtgggcat ctggctgtgt gaagccactg accccactca 
    14641 gcggggggta gataaggggc atttcccata ccaggttctc agactccagc attccacgct 
    14701 ggatacagca gaagagctga gaacagaata ggggccccag ggagacactc tcgtcccaga 
    14761 gataaagaag gcagagggag agagattcct acgcaggcga gaggccttag tctgggcctt 
    14821 gatgaacaga gacggtctat ggttttagag ctttattgta gaaaggcagg gggaaagaga 
    14881 gaaggtagaa agagagagac cagccatggc caagaggaga gaagggggga aaggagagag 
    14941 agaagaaagg ctagagagta agaaaggtta aagagacaga agtaaaagct tagagtaaga 
    15001 gagagagaga gagcaagagg gagagtaaga aaagtaaaag cttaagagag cgaggagggg 
    15061 ccagcccctc taatagtggg cttgttatct tgttgttgct aggtaactgt gaagcgtcta 
    15121 gcctgaaggc cagaagcttg ggacattgtc tatgtgactg aaagccatga gcctctactg 
    15181 tgggggctgt ggaggtggta acttggacag gagccagggg ttccaggaga catgagggaa 
    15241 tgccttctgt cccatgtagg taggaattac caccaccggc tttcaccgta gttcactgga 
    15301 gactgtttgt acatagccca ttgccccaca gaaaccaggc actcactgtt tcatttgcat 
    15361 aagaattaga aggctttatt ttggctggga ttggtgactt gccccagaaa ttccagcttt 
    15421 tgggaagccg aatcctggag aaccaggagt tcaaggtcat cctcaggtac ctcatttgtt 
    15481 caagaccagc ctgtgctacc atgagacctt gtccctactc ccctcaaccc caaaacacgc 
    15541 tgttttcatt ttataggttg tactaaccat ggaggatggt catttgcctt tctcttgatt 
    15601 tcagaagcgt agcgcgggct ttgcaccatg atctagtgtt tccagatagg cttgcctctc 
    15661 cacagccagg cttatcctgc ttcagtccca tgtttggcaa gggcaacata tgactgaaga 
    15721 acttgcatag ccttatggtg tatcatgact cttagcccca gtttataact gtctaacagc 
    15781 tggtggcttt tgtgaaaaag gaaagcatca tttgaacttt taacttttca gctgtttgtt 
    15841 ttgactttgc ataaatactg agactagcta ggaaatgagc ttttgtttca tttgctaaaa 
    15901 atgatttttt tttcatgagg caatttcaag caatttgaat ttagcaatct ctacttgtct 
    15961 gaaaagaagt tattaattaa aaaaaaacaa acaaacagga acctattatg ctggctagtc 
    16021 acatgaaaga atccaaatga acacagtcaa ggctgaatca acacatgaaa ttattttcta 
    16081 attccccaaa ttacatttat gctaattagt atgtagtaaa acattttaat tgtttccaat 
    16141 gttaaacatt acagaaataa ttttgatgtt ttcctaataa cccagccttt gatcatgttt 
    16201 gcatttattt gattgttgtc agtttattaa ataattaagt aagagcaaca agataaaatg 
    16261 acttcatgag agtccttttc tgtaacttag aacgtcagta gtcataggaa ctgcggtcgt 
    16321 tcccttacct ttcagaagta ggctaaagag ttgaacgcaa aggtggtttt tcgaatcttc 
    16381 ctgatcctta agcccccact aggtttatat tctatatttg atatcggatt cgaacccctc 
    16441 gagcttagga tccggaaccc ttaatataac taagttatta ggtccctcga agaggttcac 
    16501 tagtactggc cattgcggcc gcttcgaggc tgcctcgcgc gtttcggtga tgacggtgaa 
    16561 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg 
    16621 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg 
    16681 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga 
    16741 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat 
    16801 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 
    16861 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 
    16921 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 
    16981 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 
    17041 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 
    17101 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 
    17161 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 
    17221 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 
    17281 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 
    17341 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 
    17401 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 
    17461 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 
    17521 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 
    17581 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 
    17641 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 
    17701 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 
    17761 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 
    17821 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 
    17881 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 
    17941 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 
    18001 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 
    18061 ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 
    18121 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 
    18181 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 
    18241 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 
    18301 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 
    18361 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 
    18421 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 
    18481 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 
    18541 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 
    18601 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 
    18661 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 
    18721 gcacatttcc ccgaaaagtg ccacctgacg g 
//
