LOCUS       pAAVf-EnhCB-lacZ        7601 bp    DNA     circular     10-FEB-2012
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|617468920|
COMMENT     VNTDBDATE|617468954|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAVf-EnhCB-lacZnls mir-122 1xBS|
COMMENT     VNTAUTHORNAME|JunXie|
COMMENT     VNTAUTHORTEL|508-856-3316|
COMMENT     VNTAUTHORFAX|508-856-1552|
COMMENT     VNTAUTHOREML|jun.xie@umassmed.edu|
COMMENT     VNTAUTHORAD1|University of Massachusetts Medical School|
COMMENT     VNTAUTHORAD2|381 Plantation St., Biotech 5, Ste. 250|
COMMENT     VNTAUTHORAD3|Worcester, MA 01605|
COMMENT     VNTOAUTHORNAME|Michael Yang|
COMMENT     VNTOAUTHORTEL|8-7117|
COMMENT     VNTOAUTHOREML|miyang@mail.med.upenn.edu|
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FEATURES             Location/Qualifiers
     polyA_signal    4261..4463
                     /vntifkey="25"
                     /label=BGH-PolyA
     promoter        991..1056
                     /vntifkey="30"
                     /label=EM7\promoter
     misc_signal     1282..1311
                     /vntifkey="87"
                     /label=nls
     CDS             1057..4147
                     /vntifkey="4"
                     /label=LacZnls
     enhancer        183..463
                     /vntifkey="9"
                     /label=CMV-IE\Enhancer
     repeat_region   13..142
                     /vntifkey="34"
                     /label=itr
     misc_feature    470..735
                     /vntifkey="21"
                     /label=CB\sequence\data
                     /note="sequence data from pAAVEnhCB-lacz-Forward using primers CMV-F2 & CMV-F100"
     rep_origin      complement(4957..5412)
                     /vntifkey="33"
                     /label=f1\ori
     misc_feature    6574..7162
                     /vntifkey="21"
                     /label=COL\E1\Origin
     CDS             5543..6400
                     /vntifkey="4"
                     /label=Amp-R
     misc_feature    4213..4242
                     /vntifkey="21"
                     /label=miR-1&122a\1x\binding\site
BASE COUNT     1765 a      1981 c      2058 g      1797 t 
ORIGIN
        1 gccttaatta ggctgcgcgc tcgctcgctc actgaggccg cccgggcaaa gcccgggcgt 
       61 cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc 
      121 aactccatca ctaggggttc cttgtagtta atgattaacc cgccatgcta cttatctacg 
      181 gtcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca 
      241 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt 
      301 caatgggtgg agtattttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat 
      361 gccaagtacg ccccctattg ccgtcaatga cggtaaatgg cccgcctggc attatgccca 
      421 gtacatgacc ttatgggact ttcctacttg gcagtacatc tactcgaggc cacgttctgc 
      481 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa 
      541 ttattttgtg cagcgatggg ggcggggggg gggggcgcgc gccaggcggg gcggggcggg 
      601 gcgaggggcg gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc 
      661 tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg 
      721 cgcggcgggc gggagcggga tcagaatgat ctgatatcat cgatgaattc gagctcggta 
      781 cccggggatc ctctagagtc gaggaactga aaaaccagaa agttaactgg taagtttagt 
      841 ctttttgtct tttatttcag gtcccggatc cggtggtggt gcaaatcaaa gaactgctcc 
      901 tcagtggatg ttgcctttac ttctaggcct gtacggaagt gttacttctg ctctaaaagc 
      961 tgcggaattg tacccgcggc caaacagtag ttgacaatta atcatcggca tagtatatcg 
     1021 gcatagtata atacgactca ctataggagg gccaccatgg accctgttgt gctgcaaagg 
     1081 agagactggg agaaccctgg agtgacccag ctcaacagac tggctgccca ccctcccttt 
     1141 gcctcttgga ggaactctga ggaagccagg acagacaggc ccagccagca gctcaggtct 
     1201 ctcaatggag agtggaggtt tgcctggttc cctgcccctg aagctgtgcc tgagtcttgg 
     1261 ctggagtgtg acctcccaga ggcagttccc aagaagaaga ggaaagttga ggctgacact 
     1321 gttgtggtgc caagcaactg gcagatgcat ggctatgatg cccccatcta caccaatgtc 
     1381 acctacccca tcactgtgaa cccccctttt gtgcccactg agaaccccac tggctgctac 
     1441 agcctgacct tcaatgttga tgagagctgg ctgcaagaag gccagaccag gatcatcttt 
     1501 gatggagtca actctgcctt ccacctctgg tgcaatggca ggtgggttgg ctatggccaa 
     1561 gacagcaggc tgccctctga gtttgacctc tctgccttcc tcagagctgg agagaacagg 
     1621 ctggctgtca tggtgctcag gtggtctgat ggcagctacc tggaagacca agacatgtgg 
     1681 aggatgtctg gcatcttcag ggatgtgagc ctgctgcaca agcccaccac ccagatttct 
     1741 gacttccatg ttgccaccag gttcaatgat gacttcagca gagctgtgct ggaggctgag 
     1801 gtgcagatgt gtggagaact cagagactac ctgagagtca cagtgagcct ctggcaaggt 
     1861 gagacccagg tggcctctgg cacagccccc tttggaggag agatcattga tgagagagga 
     1921 ggctatgctg acagagtcac cctgaggctc aatgtggaga accccaagct gtggtctgct 
     1981 gagatcccca acctctacag ggctgttgtg gagctgcaca ctgctgatgg caccctgatt 
     2041 gaagctgaag cctgtgatgt tggattcaga gaagtcagga ttgagaatgg cctgctgctg 
     2101 ctcaatggca agcctctgct catcagggga gtcaacaggc atgagcacca ccctctgcat 
     2161 ggacaagtga tggatgaaca gacaatggtg caagatatcc tgctaatgaa gcagaacaac 
     2221 ttcaatgctg tcaggtgctc tcactacccc aaccaccctc tctggtacac cctgtgtgac 
     2281 aggtatggcc tgtatgttgt tgatgaagcc aacattgaga cacatggcat ggtgcccatg 
     2341 aacaggctca cagatgaccc caggtggctg cctgccatgt ctgagagagt gaccaggatg 
     2401 gtgcagagag acaggaacca cccctctgtg atcatctggt ctctgggcaa tgagtctgga 
     2461 catggagcca accatgatgc tctctacagg tggatcaagt ctgttgaccc cagcagacct 
     2521 gtgcagtatg aaggaggtgg agcagacacc acagccacag acatcatctg ccccatgtat 
     2581 gccagggttg atgaggacca gcccttccct gctgtgccca agtggagcat caagaagtgg 
     2641 ctctctctgc ctggagagac cagacctctg atcctgtgtg aatatgcaca tgcaatgggc 
     2701 aactctctgg gaggctttgc caagtactgg caagccttca gacagtaccc caggctgcaa 
     2761 ggaggatttg tgtgggactg ggtggaccaa tctctcatca agtatgatga gaatggcaac 
     2821 ccctggtctg cctatggagg agactttggt gacaccccca atgacaggca gttctgcatg 
     2881 aatggcctgg tctttgcaga caggacccct caccctgccc tcacagaggc caagcaccag 
     2941 caacagttct tccagttcag gctgtctgga cagaccattg aggtgacatc tgagtacctc 
     3001 ttcaggcact ctgacaatga gctcctgcac tggatggtgg ccctggatgg caagcctctg 
     3061 gcttctggtg aggtgcctct ggatgtggcc cctcaaggaa agcagctgat tgaactgcct 
     3121 gagctgcctc agccagagtc tgctggacaa ctgtggctaa cagtgagggt ggttcagccc 
     3181 aatgcaacag cttggtctga ggcaggccac atctctgcat ggcagcagtg gaggctggct 
     3241 gagaacctct ctgtgaccct gcctgctgcc tctcatgcca tccctcacct gacaacatct 
     3301 gaaatggact tctgcattga gctgggcaac aagagatggc agttcaacag gcagtctggc 
     3361 ttcctgtctc agatgtggat tggagacaag aagcagctcc tcacccctct cagggaccaa 
     3421 ttcaccaggg ctcctctgga caatgacatt ggagtgtctg aggccaccag gattgaccca 
     3481 aatgcttggg tggagaggtg gaaggctgct ggacactacc aggctgaggc tgccctgctc 
     3541 cagtgcacag cagacaccct ggctgatgct gttctgatca ccacagccca tgcttggcag 
     3601 caccaaggca agaccctgtt catcagcaga aagacctaca ggattgatgg ctctggacag 
     3661 atggcaatca cagtggatgt ggaggttgcc tctgacacac ctcaccctgc aaggattggc 
     3721 ctgaactgtc aactggcaca ggtggctgag agggtgaact ggctgggctt aggccctcag 
     3781 gagaactacc ctgacaggct gacagctgcc tgctttgaca ggtgggacct gcctctgtct 
     3841 gacatgtaca ccccttatgt gttcccttct gagaatggcc tgaggtgtgg caccagggag 
     3901 ctgaactatg gtcctcacca gtggagggga gacttccagt tcaacatctc caggtactct 
     3961 cagcaacagc tcatggaaac ctctcacagg cacctgctcc atgcagagga gggaacctgg 
     4021 ctgaacattg atggcttcca catgggcatt ggaggagatg actcttggtc tccttctgtg 
     4081 tctgctgagt tccagttatc tgctggcagg taccactatc agctggtgtg gtgccagaag 
     4141 taaacctgag ctagcggatc catatatagg gcccgggtta taattacctc aggtcgacgt 
     4201 cccatggcca ttcgaaacaa acaccattgt cacactccat tcgaattaaa ccgctgatca 
     4261 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc 
     4321 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg 
     4381 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg 
     4441 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag 
     4501 gcggaaagaa ccagcagatc tgcagatctg aattgcattc attttatgtt tcaggttcag 
     4561 ggggaggtgt gggaggtttt ttcggatcct ctagagtcga ccgtagataa gtagcatggc 
     4621 gggttaatca ttaactacaa ggaaccccta gtgatggagt tggccactcc ctctctgcgc 
     4681 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg 
     4741 gcggcctcag tgagcgagca gtgagcgagc gagcgcgcag ccttaattaa cctaattcac 
     4801 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc 
     4861 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc 
     4921 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa 
     4981 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 
     5041 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 
     5101 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 
     5161 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 
     5221 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 
     5281 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct 
     5341 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa 
     5401 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 
     5461 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 
     5521 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 
     5581 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 
     5641 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 
     5701 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 
     5761 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 
     5821 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 
     5881 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 
     5941 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 
     6001 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 
     6061 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 
     6121 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 
     6181 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 
     6241 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 
     6301 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 
     6361 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 
     6421 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 
     6481 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 
     6541 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 
     6601 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 
     6661 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 
     6721 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 
     6781 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 
     6841 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 
     6901 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 
     6961 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 
     7021 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 
     7081 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 
     7141 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 
     7201 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 
     7261 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga 
     7321 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg 
     7381 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg 
     7441 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct 
     7501 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta 
     7561 tgaccatgat tacgccagat ttaattaagg ccttaattag g 
//
