LOCUS       Ple120\(pEMS1389       18264 bp    DNA     circular     11-FEB-2010
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|545675448|
COMMENT     VNTDBDATE|545675448|
COMMENT     LSOWNER|
COMMENT     VNTNAME|Ple120 (pEMS1389)|
COMMENT     VNTAUTHORNAME|Demo User|
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COMMENT                (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #141=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 
COMMENT                "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 
COMMENT         (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 
COMMENT          (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 -365) 
COMMENT        1 1 0 1 1 
COMMENT        (mapper: 23.2635 -18.7677 40.0378 40.0378 0.01 10 -1 18264 18264 1 0 0) 
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COMMENT                            0.871165 0 -10 10.5154 1.44834 Nil) 
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COMMENT               #147=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -1092 100) 
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COMMENT                                   -11.9161 -6.96932 0 -10 2.63876 1.15074 #149#) 
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COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.59929 1.9 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "MFRT (F5mutant)" "@N" 1 12.3322 11.1344 0 
COMMENT                                   -10 7.34091 1.15074 #151#) 
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COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.58761 1.9 
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COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
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COMMENT                                   "Kozak consensus" "@N" 1 13.8053 9.46696 0 
COMMENT                                   -10 7.53931 1.15074 #153#) 
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COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Tetracysteine residue" "@N" 1 18.5443 
COMMENT                                   4.73348 0 -10 9.16621 1.15074 #157#) 
COMMENT                             #159=(CScratch 
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COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.32554 1.9 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "NLS" "@N" 1 
COMMENT                                   15.7929 2.6165 0 -10 1.78563 1.15074 #159#) 
COMMENT                             #161=(CScratch 
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COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.29875 1.9 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "MFRT" "@N" 1 
COMMENT                                   15.9265 0.27474 0 -10 2.48004 1.15074 #161#) 
COMMENT                             #163=(CScratch 
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COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.23452 1.9 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 early polyA signal" "@N" 1 16.7258 
COMMENT                                   -1.74233 0 -10 10.059 1.15074 #163#) 
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COMMENT                                   "Ple120 (HTR1A-D)" "@N" 1 9.39982 14.5441 0 
COMMENT                                   -10 7.57899 1.15074 #165#) 
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COMMENT                                   "@N" 1 9.72997 12.6519 0 -10 5.55528 1.15074 
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COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                             #170=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "Region 1" "@N" 
COMMENT                                   1 6.39487 16.4363 0 -10 3.76965 1.15074 #169#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #171=(CGroupWidget 
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COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 1 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "AmpR" "@N" 1 
COMMENT                                   -4.60038 12.1963 0 -10 2.61892 1.15074 #172#) 
COMMENT                             #174=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #2# 0) 1 2 0 0 #119# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 0.835356 
COMMENT                                    2.11682 2.60661 1 0.082322) 0.8 1.8 0) 
COMMENT                             #175=(CLabel 
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COMMENT                                   -10.2211 -8.71165 0 -10 2.61892 1.15074 #174#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #176=(CGroupWidget 
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COMMENT                                   "5' homology sequence" "@N" 1 -16.2675 
COMMENT                                   3.06608 0 -10 9.58286 1.15074 #177#) 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "3' homology sequence" "@N" 1 6.41813 
COMMENT                                   -12.1963 0 -10 9.58286 1.15074 #179#) 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "HPRT complementary sequence" "@N" 1 18.3954 
COMMENT                                   -3.48466 0 -10 13.6303 1.15074 #181#)) 
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COMMENT               #183=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -185 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #184=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil 1055 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #185=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -116 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 
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FEATURES             Location/Qualifiers
     misc_recomb     12127..15938
                     /vntifkey="86"
                     /label=5'\homology\sequence
     misc_marker     complement(17037..18258)
                     /vntifkey="22"
                     /label=AmpR
     misc_marker     10676..12101
                     /vntifkey="22"
                     /label=AmpR
     misc_recomb     7035..10680
                     /vntifkey="86"
                     /label=3'\homology\sequence
                     /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3"
     misc_recomb     3081..7034
                     /vntifkey="86"
                     /label=HPRT\complementary\sequence
                     /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2"
     misc_feature    12108..12125
                     /vntifkey="21"
                     /label=I-Sce\I
     misc_feature    1963..1996
                     /vntifkey="21"
                     /label=MFRT\(F5mutant)
     misc_feature    1997..2007
                     /vntifkey="21"
                     /label=Kozak\consensus
     misc_feature    2004..2723
                     /vntifkey="21"
                     /label=EGFP
     misc_feature    2724..2759
                     /vntifkey="21"
                     /label=Tetracysteine\residue
     misc_feature    2760..2832
                     /vntifkey="21"
                     /label=NLS
     misc_feature    2838..2871
                     /vntifkey="21"
                     /label=MFRT
     misc_feature    3025..3075
                     /vntifkey="21"
                     /label=SV40\early\polyA\signal
     misc_feature    133..1948
                     /vntifkey="21"
                     /label=Ple120\(HTR1A-D)
                     /note="1306"
     misc_feature    1181..1948
                     /vntifkey="21"
                     /label=Region\Prom
     misc_feature    133..1180
                     /vntifkey="21"
                     /label=Region\1
BASE COUNT     4888 a      4059 c      4162 g      5155 t 
ORIGIN
        1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 
       61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 
      121 cgagctccta ggttactaga gaagcaggct actggtatct ccccctttct ctgcctcgca 
      181 gagaggccag tgtgtgcttc taagtgaact tcctgtggct ctcatttcct aaacttgaag 
      241 cagttcgttt ctgataggga tgtgagaatt gaagccccag gatacgacca aattagactt 
      301 tggcccacaa tagcagacca aagttagtat atttttcaca gcttgattag aagagcttgc 
      361 atctaggaag ggcaggatta ttacatctga cttccaggag agcagctgtg gtattattct 
      421 ccagcaagct aacaacagag aatctgctct ggttgacagc acatttcttt actccagccc 
      481 agatctggca gccttgcctg agtacctttc taatgccaga gccctcagct ctccagctgt 
      541 cttcagttgc tgtccttttc cttcagctgt catgactctg ctgtgatctt tgaaatttgc 
      601 ctgcaaacat atattttctt aattctattt tctctccaaa gtggtcaaag ggcccttgca 
      661 tttgtatgag gatcaaaaag gaacttttat atgttacata caaaaaaagg ggcagctttt 
      721 cataaactcc tattttccat tggctggtgg gccactggtc tcagaatcat gtaggaggct 
      781 tgataaatag acacatcctt gatccaccct agaactactc actcaaagtc actggaagtt 
      841 gaggcctggg agtctgcatt tttaccaagc tcatcaagtg atttttatgc acagtcaggt 
      901 ttgaaagcca cattaatttg ttccattgta caagttttct atagttttct ccttttttct 
      961 caatgagaag gtggaaagga ctcactgaaa tgtctatttc tatatggatt ttgagtgaag 
     1021 ccaggatcaa agcagatttg ctatgcagtg tttctcatta aatacaaggt caaggaggaa 
     1081 ataactcatt tactttacat ggtagatgac cacaaagact ttcttgtttg tggcatattc 
     1141 tgttgcagtt ggttggttaa cttttcattg cctttcagaa ttacaacctt ggtctgaccg 
     1201 gcagggatct ggtgtgtgta agtgagttct gagtctctgt tgacaaaaag agactcgaat 
     1261 gcaaagacgc tgagctagag ggagaggagg gcggggaccc agaggaaaga ggcactcctc 
     1321 ggggttgggg aagtattagg aggggagggt tagagtggga gggaaggagc ctggctttcg 
     1381 aagcgactca cagagggata aataaaggga agtgaggagg aagagggaga ctgaaaggga 
     1441 aggcaggtgg ggagaagggg gacgaaagag gcagaagaga gagaagagag gaggagagag 
     1501 ggggagagag ggaaggaagg aaatagggag aggagggtca cagagtgacc gtggaggatg 
     1561 gggcttctcg gttctagata tttctgggat tggagactgt ttgctagtgg ggagactcca 
     1621 gctccggcag ccagttcggg agcggcaaag taaaatggac agcgacagac agacgttcca 
     1681 gccacctctc cgccgccggg agatcctgga gctgctttca ggccaactcc agtttcccag 
     1741 ctggagcttc tgaacgcgct ggactgcgag agcccaggga gcgcctgaaa gctgctcctc 
     1801 ggagataccc ttcgccgaag cagtaagaac ttcctgcttg ggtctctgca ttcccttcct 
     1861 ccgaaacttc ccaggagaag ggcggaagac cccaggggaa ggggcgaggc gaatcttcgc 
     1921 gctgcttttt cttccctccc ccttcccgac gcgtggcgcg ccgaagttcc tattcttcaa 
     1981 aaggtatagg aacttccgcc accatggtga gcaagggcga ggagctgttc accggggtgg 
     2041 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg 
     2101 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca 
     2161 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca 
     2221 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct 
     2281 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg 
     2341 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg 
     2401 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata 
     2461 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg 
     2521 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc 
     2581 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca 
     2641 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg 
     2701 gcatggacga gctgtacaag ttatttctga actgctgccc gggctgctgc atggaaccgg 
     2761 atccaaaaaa gaagagaaag gtagatccaa aaaagaagag aaaggtagat ccaaaaaaga 
     2821 agagaaaggt aatataagaa gttcctattc tctagaaagt ataggaactt cagcggccgc 
     2881 gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc 
     2941 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt 
     3001 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 
     3061 catttttttc actgcgaatt ctcgtaaaac tcttcatggc agtagttatt attctctctc 
     3121 tctctctttt tcttttttct tgagacagga tatttctctg ttgcccaggc tggagtgcag 
     3181 tggcacagtc ttggctcact gcagcctgga cctcctgggc tcaagccatc ctcccacctc 
     3241 agcctcccaa gtagctgggg ctacaggcac atggccacca ggcccagata atttttcatt 
     3301 tttgtagaga ctgagtctca ccatgttacc caggtttatt attctcattt tttagatgaa 
     3361 gagactgagg tccagagaag ctcaatgact tgcctagttt tacaaatctc ctgccatcac 
     3421 atacccctca gcgtccttaa taagagggag gccaccaact atgtgctggg cactgtggtg 
     3481 gatgctggag ctatagggtt gagtatataa gaaatggtgt tgctggagca actgttgctt 
     3541 gcttacctga cctatctgag aattaattag caggggaaca tatttttgtt ttcagattca 
     3601 atataagaac ttgtgtgggc aaaaataaag atcagtagta ataacagtag ttcccatttg 
     3661 ctgactgtac tgtcctaagt gcatatatat atacatacac acacgcatac ctatactcct 
     3721 ctaatactca aaatgatcct gtttatgtat tgttaatatg ctcattttat ttttaaattt 
     3781 ttatttattt ttatttttat ttatttttga gacggagtct cattctgtcg cggaggctga 
     3841 agtgcagtgg tgcgatctca gctcagtgcg acctccgcct cccgggttca agtgattctc 
     3901 ctgcctcagc ctccggacta gctgggatta caggcgcccg cctccacgcc cagctaattt 
     3961 ttgtattttt agtagagatg gggtttcgcc atgttggcca ggctggtctc gtactcctga 
     4021 ccttgagtga tccacctgcc tcggcctccc aaagtgctgg gattacaggc atgagccacc 
     4081 gcgccgggct aatatgctca ttttagtgag gcaaaaatag aggctcagag tctgatttgt 
     4141 acaaaactac agagcagtta agtgtcctct cagatgtgta ccctgatctg ggtgactcta 
     4201 ggactctagg tctcaactgt tacaaccagt taagggtttg gggaagcact gggccaagag 
     4261 tcaggaaaat ggaagccaca ggtagtgcaa ggtcttggga atgggacgtc tggtccaagg 
     4321 attcacgcga tgactggaac ccgaagagcc ggggcccggt ttacggccgc catgaagcaa 
     4381 cgcgcgccgg taggtttggg aatcagggag ccctctgaat aggagactga gttgggaggg 
     4441 aaaggggctt cgctggggga gcctcggctt cttctgggag aaaattccca cggctaccta 
     4501 gtgagcctgc aaactggtag gcgccggcgt aggcgcgcgg gcggggccgg gggcggggcc 
     4561 tgcggggcgt ggcggggcgg gcagagggcg gggcctgctt ctcctcagct tcaggcggct 
     4621 gcgacgagcc ctcaggcgaa cctctcggct ttcccgcgcg gcgccgcctc ttgctgcgcc 
     4681 tccgcctcct cctctgctcc gccaccggct tcctcctcct gagcagtcag cccgcgcgcc 
     4741 ggccggctcc gttatggcga cccgcagccc tggcgtcgtg gtgagcagct cggcctgccg 
     4801 gccctggccg gttcaggccc acgcggcagg tggcggccgg gccctgaggc gcgggatcga 
     4861 tccatgacac ccccccgccc cccatatctc actataattc actgtcaaat ggcttttatg 
     4921 atttggagtt tggggatact tttaaagaca gggtcttggt agctcctaga tggcttggga 
     4981 ttcacttagt aacccttgct ggcctaaaat tcaggaactt ctacctcatc ttcccaagta 
     5041 ctgggttagt agggatgtcc agcatattta gcttgcttta atgattttgc tgtacttttt 
     5101 tctgtgtgta caggtgcatg tatatttata aaactaacat aatatatttt acccccaaag 
     5161 taccataacc ctaattaatg ctattaaatg ttttgtatgc tgggctctgg gcatactaat 
     5221 ataaccaata ttatacaatg caaaacaatt ttggaatatc ttctgtaaaa ttatcttatt 
     5281 agtaagttgt aaccatctca gacttctaag tttgagtttg taaaggatca gttttttttg 
     5341 gtgacctaca cacacacaca cacacacaca cacacacaca cacacagggg ggtggattct 
     5401 gagaatttag agaatttaga tggttaatac cagtttttat ttttattctg tgtgatattt 
     5461 atttattaag atttatttat ttattacatg taggtacact gtatctgtct tcagacacac 
     5521 cagaagaggg catcagatct cgttacagat ggttgtgagt caccatgtgg ttgctgggat 
     5581 ttgaactcag gacctctgga agaacagtca gtgctcttaa ccgttgagcc atctcaccag 
     5641 ccccgtgata ttttatttaa cttactagaa tatactgtgt taaaacatat tttaggggat 
     5701 tggggagatg gcccattctg taaagtgtct gccatgtcat cttcaggacc tgcgtttgta 
     5761 accccagcat ggagaagaca gagacaggga gacccctggg cttcctggca agctagacta 
     5821 gcaaatttgg ttaactaggt tcaatgagag actcagtctc aaaacataag aagtgaattt 
     5881 tttagaccca atttcaatct ctgaactcca tacgtatgca catgtacctg catataaata 
     5941 tatatacaca cccataaaca catgcacaca caaaaaatgg ttcaaaagtt cttaaaatgt 
     6001 ataagagtag aaagtgtaga gccgggagtg gtggcgcacg cctttaatcc cagcacttgg 
     6061 gagcagaggc aggtggattt ctgagttcga ggccagcctg gtctacagag tgagttccag 
     6121 gacagccaga gctatacaga gaaatcctgc ctcaaaaaac caaaaataaa gtgtatatac 
     6181 taatctttca tgcctaaaat atttattttt gccacatttt tcatacctct ttaatatttt 
     6241 tctttttctc tttgtgcttc aaaggaaatt ttgtgtcatg ttttactttt cattcttgac 
     6301 tctattcagc agtaagacgc agcatggctt tcttagagaa gtataatcta ttacaaaacg 
     6361 gtaacattta actgctctac ataccctctt gtagctaaat tcctctgtta aactaagact 
     6421 tattatctaa acgtaattaa atgttgatat cttataatgc tccactttga aacagctgtc 
     6481 tttcttttgc agattagcga tgatgaacca ggttatgacc tagatttgtt ttgtatacct 
     6541 aatcattatg ccgaggattt ggaaaaagtg tttattcctc atggactgat tatggacagg 
     6601 ttagtaagac ctcgattgaa gttattttta gagggtgtgt gtgtggtgtg tgtgtgtgtg 
     6661 tgtgtgtgtg tgtgtgtgtg tgtgtaagag agagaggaga ggcatgtgtg atatatatgt 
     6721 gaagtgtatt gcatgtacat ggaatccaga gaaggacatt aggtgtccta ctctgttgct 
     6781 ctcttattcc cttgaatcag tgtctcttgc tgaacctggg gcatacatac tgatttggct 
     6841 aggctgcctg gctagcaaga cccagggatc tgcttgtctc tgccccccag tgctgacgtt 
     6901 acaagcaggt gcaaccacat ctggttttta tatgggtact gggggatctg aacttagggc 
     6961 ctgatgcgtg cccaagtgct ctcacccgcc aagccacctc tccagccaaa gggttttttt 
     7021 tcttgtatta attctaactt agcaactgtt gagtagttga tgtttgaata taagctaagc 
     7081 caacttatta gaataaatgt tttctatttt ttacttgtat gtgtgagtgc ctgcatgtat 
     7141 gtatgtgaag catatgtatt ccaggatgtc agaattgtga tggattccct ggaactggag 
     7201 ttacagacca ttgtgaacca ctgtgtatgg gtgctgggaa ctgaacttta ggtcctctgc 
     7261 aaaaacagta aggatcttaa ctgataaact atctttctag ccctaaggct ttctactttt 
     7321 aaagttcgtt tctctttctc tctctctctc tctctctctc tctctctctc cctctccctc 
     7381 cctcctcctc ctcctcctct ttcccccctc tcgcctcctc tttctctctc ccctccctct 
     7441 ctctctctca cacacacaca cagacagaga gaggagagag aaaggaagcc ttactaatta 
     7501 attacctagt tttagacata actagtggga atccatagta agtaaatcaa cattcttgaa 
     7561 gagaggttag aagaattggc tctcgttgtc catcgaggct gtataagatt attcttctgt 
     7621 tttctcagct taccatacat attttctacg tagacaatac caattcaaat acactgtacg 
     7681 taaagtatat gcgcacacac acacacacac acacacacac acacacacaa gttacatatt 
     7741 gtcatatagt actaaaattt atggactttt tcatattaat acatctagag ctatcatgtt 
     7801 ttttatcttt taaaaattac tttacggggc tacagagatg tctcagcagt taggagcact 
     7861 gactgctctt ccagaggtcc tgagttcaat tcccagcaac cacatggtgg ctcacaacca 
     7921 tctgtaatgg gatctgatcc actcttatgg tgtgtctgaa gacagtgaca gtgtactcat 
     7981 atacatgaaa taaatcttta aaaaattact ttaccatcag ggtatatata tatatatata 
     8041 tgtgtgtgtg tgtgtgtgtg tgtatgaagt atttgctatt tgagagggtc tcactgttta 
     8101 gctcagtctg gcttggaacg ttctgtatag cccagactgg cctcaactca gcagtccccc 
     8161 tgaaaggctc tcatatgtgg agattacagg tatgtgctac tgtgcccagc cctacatgtt 
     8221 actttgtttt tgaaataggg tatcaaatag cttagactgg cttctaagtc agtgtgtagc 
     8281 taaggatggt ctgagcttct gaacctgcta cttcaactcc tggtgtgcca tcatggccag 
     8341 tttgcacagt tgtgagagta gaactcagga cctcctatgt gttactagac atgttctcta 
     8401 ccagctgagc tacatcccaa ttcgcctaca tttactttta ttttttaaaa gatttattta 
     8461 tgtatacagc actctgcctg catgtacatc tgcatgccag aagagggcac cagatctcat 
     8521 catacatagt tgtgagccac catgtggttg ctggggattg aactcagggc ctctggaaga 
     8581 gcagtcagtg ctcttaacct ctgagccatc tctgtagccc cctacattta ttttaaagat 
     8641 tataggctat tctattcata gatgtgccat aactgattta accagatctt cattattttg 
     8701 tacttagtag agtatattag agggaatgaa tcttaaatac acattttgac acttgctcag 
     8761 ctttagacaa gtaaaaatca ctggtcaagg aaatggtgct tagtggatgc ttctgaactg 
     8821 tcaaattgtc ctccaacaag ttacatcatt ttatacttcc aacgttatgt gagagttcat 
     8881 ttttctttag gctcttctag tattttatat ttgagaaata aaatttaacc aaaatttgcc 
     8941 aatctagtaa gcttgttttt atttgtatct ttaaatttta tgatagatgt tatagtgtac 
     9001 tctcctctcc cctcccctcc cctctcctcc tcagcaggat cttgttatgt agccctaagg 
     9061 gatctaatac ttactctgta gtccaggctg tccttcagct catgagacac accatcatgc 
     9121 ttggcatgtt tcttgttctg aaaggtatct ggttttaatt ttggttgagc tggtgtgttg 
     9181 gtgtatatct gtaatctcag cacaggcaag tagagacaag gattatgatt acagtccagc 
     9241 ctgggctatt tagagggatt ctctttaatc tttcctcatg ccccaaaatc ttacctttgg 
     9301 tatatgaaaa atagtctcca cttctgcaaa atattgcttt atgaagtaag aattaattcc 
     9361 cttcatagag acaaggaatg tgtcctgtaa aagtttaatg tgtaagaagt atttgttata 
     9421 aaagataaat attcagaatc ttctttttaa ttcctgattt tatttctata ggactgaaag 
     9481 acttgctcga gatgtcatga aggagatggg aggccatcac attgtggccc tctgtgtgct 
     9541 caaggggggc tataagttct ttgctgacct gctggattac attaaagcac tgaatagaaa 
     9601 tagtgataga tccattccta tgactgtaga ttttatcaga ctgaagagct actgtgtaag 
     9661 tataattaac ttataattaa aaaaataggg ccattctagt tttatctata ttttttttaa 
     9721 acttgtgcaa actatgctac ataatcaact tacttgatta tgtaatatat atagtaatag 
     9781 ttggaattac tattattaca tatttatttt tctgtgctgg ggtagaaccc agggaactaa 
     9841 gtactgtaat aaccgagctt cattcatcct agtcattttt aatttttttt tttttttttg 
     9901 ttttttcgag acaaggtttc tctgtgtagc cctggctgtc ctggaactca ctctgtagac 
     9961 caggctggcc tcgaactcag aaatctgcct gcctctgcct cccagtgctg agattaatga 
    10021 cgtgcaccac cactgcctgg cattttttta aagttttaat ttgagaccct ttctcactaa 
    10081 cttgctcaca ctatcctcga actcattacg tgggctggtc tagaaatttc agtcaacttg 
    10141 cctcagcttc tcaagtagct gggattacag gcaggtgcta ccacttctgg ccatgatcat 
    10201 tgttgttgtt gctattatta ttattattat ttcactgata cttcatatca caacacagaa 
    10261 tagtaactgt agttcatgac caacaaaaaa gttgtacagt ttctctgttg tcttattttg 
    10321 atctggattt tgatttgttt catggcactg ggcaggctaa tctcaaactg catggctcaa 
    10381 atagtccttc tgtcttaatc tcccatgttc tggaagtata ggtatcacta cacctagctg 
    10441 tattttcata ttttaaaaaa aacgcctaag aattgctatt gaatgaggcg gagagatggc 
    10501 tcagcagtca caagtacttg tagaggactg gggtttggtt actagcacct caaagatggc 
    10561 ttacaattat ctgtagctcc aattccagag gatctgtgtg gtaaagctgc atgtatatgg 
    10621 gcatgcaggt aaaactgcta aacatataaa aatagctcag tcttaagaga attggtcgac 
    10681 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg 
    10741 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt 
    10801 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa 
    10861 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 
    10921 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 
    10981 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 
    11041 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 
    11101 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 
    11161 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 
    11221 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg 
    11281 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 
    11341 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 
    11401 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 
    11461 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 
    11521 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 
    11581 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 
    11641 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 
    11701 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 
    11761 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 
    11821 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 
    11881 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 
    11941 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 
    12001 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 
    12061 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca ggtcgagtag ggataacagg 
    12121 gtaatggatc ctcgttgggc acctgcactc atgtacgcag gcccacatat agatgtacac 
    12181 acgcatatca ttcaaaatag aaattattta tacaagtagt gcgttggaga gctggctcag 
    12241 gagttgttaa tgcttgctac ccttgcaaag gacccaagtt caatttccag cacctgtgtt 
    12301 gggcatctca caaccacatg taaatctagt ttcaggggat ttcacccctc ttctgaactc 
    12361 ttcaggcact tatacccaca aatggacatc tccacataga gacacatact tttaaaaaag 
    12421 taatctaggg tctagagaaa tggttataga ccacggactg ggatcagttc ccagaagcca 
    12481 catcctgtgg ctcacaattg cctgtgaatc aagttctaga tctggtggcc tcgtctagcc 
    12541 tgcagtcatc aacaaacaca tgcacacatc catacacaca taatttaaaa gaaagcgaaa 
    12601 gtaacctata atgctaaaca aagcacattc tcagtgtaga ctgcagtggt gatgaacctt 
    12661 gaaaatatat taatagaagc cagacacgta agtcacaaat ggtataactc aatttatatg 
    12721 aaattcccag agagtccaaa gttaaaaaaa acaaaaaggc agattattgg ttgtcagtag 
    12781 ctacagagat gggacattga agatattaat aggtataggg ttgaggattc atggcctttc 
    12841 cctcttccat gttagcatgt ctattgttgt taaccttatt caactcatgt tagggcaatc 
    12901 atattgatga gacttaatga gtatagcttg acattcctag aagacagagt ctcacatcag 
    12961 actctctgat cctctgactc ttagaatctt tcaacaccct cttctgaaat gatctctgag 
    13021 cctttggttg tgactgaatt atgagaaaga attttatgtt gaatgaattt tacttcagca 
    13081 tttttacaaa actgaagctg gggatggcgt ttcatgcgtg cagtccctac catttgccta 
    13141 taagcctgga ggaggcagaa gtagaattag gcttttcagg gacatcttca gctgcatcat 
    13201 gagcttgagg acaacttgag ctacataaga ttcccatccc cacccccaca cacacacaca 
    13261 catctagtat gtacctagta tgcagtcttt tcatatgttt aaaatgtaga aaggtatatg 
    13321 tgaagtctcc cttttcccca caacttagtc cctccaaagt attgcatcag tttccttgta 
    13381 catgatttta tgcatatata agatacacta tttctctctc tctctctctc cttccctcct 
    13441 tccctccaac ctctgggtat gagttgaggg ttatgtatgt ttcaagttgt aataagaatt 
    13501 gccactcaaa catttcatcc cattttaagt taagtttgtc tgattttcca ctttagaagg 
    13561 gcagccatga tggctcatgc ctataatccc atcctagcac ttgtaagcct aatacataag 
    13621 actggaggat tgtcatcagc ttgaggctag cctggtctac ctaatgagtt ccagccagcc 
    13681 aagagtagaa tataagaccc tgtctccaaa aaatgtacca aaggggctgg aggggtaact 
    13741 cagtggttaa gaacagtagc tgctcttcca gaggacctgg gttcaattcc cagcacccac 
    13801 atggcagctt gcaactatct gtaactgtcc agctttggga tatccgacac cattatacca 
    13861 attacataaa atgaaaaaat tgtaaaatgt gctaaaatga gtggggaggg ggagcatggt 
    13921 gtggttctgt gggcttggga ttttgccatg aatccatgac tatatctcag caatatgtgg 
    13981 attatttctg gaaacctgtg gggcatgggt gtatgggaag aggggagaga gagagagccc 
    14041 gcatccagcc agagttcctg tgctctgggc aggcggacgt gggaggactg ctggacgatt 
    14101 tccacttggc cctgggtggg catctggctg tgtgaagcca ctgaccccac tcagcggggg 
    14161 gtagataagg ggcatttccc ataccaggtt ctcagactcc agcattccac gctggataca 
    14221 gcagaagagc tgagaacaga ataggggccc cagggagaca ctctcgtccc agagataaag 
    14281 aaggcagagg gagagagatt cctacgcagg cgagaggcct tagtctgggc cttgatgaac 
    14341 agagacggtc tatggtttta gagctttatt gtagaaaggc agggggaaag agagaaggta 
    14401 gaaagagaga gaccagccat ggccaagagg agagaagggg ggaaaggaga gagagaagaa 
    14461 aggctagaga gtaagaaagg ttaaagagac agaagtaaaa gcttagagta agagagagag 
    14521 agagagcaag agggagagta agaaaagtaa aagcttaaga gagcgaggag gggccagccc 
    14581 ctctaatagt gggcttgtta tcttgttgtt gctaggtaac tgtgaagcgt ctagcctgaa 
    14641 ggccagaagc ttgggacatt gtctatgtga ctgaaagcca tgagcctcta ctgtgggggc 
    14701 tgtggaggtg gtaacttgga caggagccag gggttccagg agacatgagg gaatgccttc 
    14761 tgtcccatgt aggtaggaat taccaccacc ggctttcacc gtagttcact ggagactgtt 
    14821 tgtacatagc ccattgcccc acagaaacca ggcactcact gtttcatttg cataagaatt 
    14881 agaaggcttt attttggctg ggattggtga cttgccccag aaattccagc ttttgggaag 
    14941 ccgaatcctg gagaaccagg agttcaaggt catcctcagg tacctcattt gttcaagacc 
    15001 agcctgtgct accatgagac cttgtcccta ctcccctcaa ccccaaaaca cgctgttttc 
    15061 attttatagg ttgtactaac catggaggat ggtcatttgc ctttctcttg atttcagaag 
    15121 cgtagcgcgg gctttgcacc atgatctagt gtttccagat aggcttgcct ctccacagcc 
    15181 aggcttatcc tgcttcagtc ccatgtttgg caagggcaac atatgactga agaacttgca 
    15241 tagccttatg gtgtatcatg actcttagcc ccagtttata actgtctaac agctggtggc 
    15301 ttttgtgaaa aaggaaagca tcatttgaac ttttaacttt tcagctgttt gttttgactt 
    15361 tgcataaata ctgagactag ctaggaaatg agcttttgtt tcatttgcta aaaatgattt 
    15421 ttttttcatg aggcaatttc aagcaatttg aatttagcaa tctctacttg tctgaaaaga 
    15481 agttattaat taaaaaaaaa caaacaaaca ggaacctatt atgctggcta gtcacatgaa 
    15541 agaatccaaa tgaacacagt caaggctgaa tcaacacatg aaattatttt ctaattcccc 
    15601 aaattacatt tatgctaatt agtatgtagt aaaacatttt aattgtttcc aatgttaaac 
    15661 attacagaaa taattttgat gttttcctaa taacccagcc tttgatcatg tttgcattta 
    15721 tttgattgtt gtcagtttat taaataatta agtaagagca acaagataaa atgacttcat 
    15781 gagagtcctt ttctgtaact tagaacgtca gtagtcatag gaactgcggt cgttccctta 
    15841 cctttcagaa gtaggctaaa gagttgaacg caaaggtggt ttttcgaatc ttcctgatcc 
    15901 ttaagccccc actaggttta tattctatat ttgatatcgg attcgaaccc ctcgagctta 
    15961 ggatccggaa cccttaatat aactaagtta ttaggtccct cgaagaggtt cactagtact 
    16021 ggccattgcg gccgcttcga ggctgcctcg cgcgtttcgg tgatgacggt gaaaacctct 
    16081 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac 
    16141 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt 
    16201 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact 
    16261 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 
    16321 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 
    16381 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 
    16441 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 
    16501 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 
    16561 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 
    16621 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 
    16681 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 
    16741 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 
    16801 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 
    16861 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 
    16921 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 
    16981 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 
    17041 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 
    17101 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 
    17161 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 
    17221 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 
    17281 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 
    17341 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 
    17401 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 
    17461 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 
    17521 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 
    17581 tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 
    17641 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 
    17701 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 
    17761 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 
    17821 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 
    17881 gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 
    17941 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 
    18001 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 
    18061 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 
    18121 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 
    18181 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 
    18241 tccccgaaaa gtgccacctg acgg 
//
