LOCUS       pEMS2058\ssAAV\CAGGS-emGFP\WPRE        6693 bp    DNA     circular      2-MAY-2013
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|643304842|
COMMENT     VNTDBDATE|661017630|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEMS2058 ssAAV CAGGS-emGFP WPRE|
COMMENT     VNTAUTHORNAME|Jack Hickmott|
COMMENT     VNTOAUTHORNAME|User|
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FEATURES             Location/Qualifiers
     CDS             4646..5503
                     /vntifkey="4"
                     /label=Amp-R
     misc_feature    5677..6265
                     /vntifkey="21"
                     /label=Origin
     misc_feature    3708..3753
                     /vntifkey="21"
                     /label=additional\AAV\sequences
     repeat_region   1..168
                     /vntifkey="34"
                     /label=5'\ITR
     repeat_region   complement(3716..3883)
                     /vntifkey="34"
                     /label=3'\ITR
     misc_feature    197..208
                     /vntifkey="21"
                     /label=MCS\5'\end
     intron          1944..2116
                     /vntifkey="15"
                     /label=Intron
     enhancer        2863..3449
                     /vntifkey="9"
                     /label=WPRE
     promoter        209..1943
                     /vntifkey="29"
                     /label=CAGGS
     exon            2117..2856
                     /vntifkey="61"
                     /label=emGFP
     primer          2508..2528
                     /vntifkey="27"
                     /label=oEMS5955
     primer          complement(2245..2265)
                     /vntifkey="27"
                     /label=oEMS5954
     polyA_signal    3450..3701
                     /vntifkey="25"
                     /label=SV40\pA
BASE COUNT     1384 a      1846 c      1913 g      1550 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct 
      181 aggaagatcg gaattcccta ggccggccac gcgttgacat tgattattga ctagttatta 
      241 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata 
      301 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat 
      361 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga 
      421 ctatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc 
      481 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt 
      541 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatgggtcg 
      601 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt 
      661 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggcgc 
      721 gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg 
      781 gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg 
      841 cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgttgcc ttcgccccgt 
      901 gccccgctcc gcgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca 
      961 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga 
     1021 cggctcgttt cttttctgtg gctgcgtgaa agccttaaag ggctccggga gggccctttg 
     1081 tgcggggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg 
     1141 cggcccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc 
     1201 cgcgtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg ggggctgcga 
     1261 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc 
     1321 ggcggtcggg ctgtaacccc cccctgcacc cccctccccg agttgctgag cacggcccgg 
     1381 cttcgggtgc ggggctccgt gcggggcgtg gcgcggggct cgccgtgccg ggcggggggt 
     1441 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg 
     1501 aggggcgcgg cggccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg 
     1561 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctggcggag 
     1621 ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcgggcgaag cggtgcggcg 
     1681 ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc 
     1741 tccatctcca gcctcggggc tgccgcaggg ggacggctgc cttcgggggg gacggggcag 
     1801 ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca 
     1861 tgccttcttc tttttcctac agctcctggg caacgtgctg gttgttgtgc tgtctcatca 
     1921 ttttggcaaa gaattggcgc gccgaagttg gtcgtgaggc actgggcagg taagtatcaa 
     1981 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact 
     2041 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac 
     2101 aggtgtccac tcccaggcgg ccgccaccat ggtgagcaag ggcgaggagc tgttcaccgg 
     2161 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc 
     2221 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac 
     2281 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcacctacg gcgtgcagtg 
     2341 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga 
     2401 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc 
     2461 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt 
     2521 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaaggt 
     2581 ctatatcacc gccgacaagc agaagaacgg catcaaggtg aacttcaaga cccgccacaa 
     2641 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga 
     2701 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga 
     2761 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac 
     2821 tctcggcatg gacgagctgt acaagtaagc ggccgcgatc gctcaacctc tggattacaa 
     2881 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata 
     2941 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc 
     3001 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg 
     3061 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac 
     3121 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat 
     3181 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt 
     3241 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat 
     3301 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc 
     3361 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag 
     3421 tcggatctcc ctttgggccg cctccccgcg atcgcagaca tgataagata cattgatgag 
     3481 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat 
     3541 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc 
     3601 attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaaag caagtaaaac 
     3661 ctctacaaat gtggtagtcg aaattcccga taaggatctt cctagagcat ggctacgtag 
     3721 ataagtagca tggcgggtta atcattaact acaaggaacc cctagtgatg gagttggcca 
     3781 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc 
     3841 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagccttaat taacctaatt 
     3901 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc 
     3961 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc 
     4021 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat 
     4081 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag 
     4141 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc 
     4201 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc 
     4261 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt 
     4321 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa 
     4381 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg 
     4441 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat 
     4501 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 
     4561 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 
     4621 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc 
     4681 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa 
     4741 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg 
     4801 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt 
     4861 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg 
     4921 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac 
     4981 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc 
     5041 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa 
     5101 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc 
     5161 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt 
     5221 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga 
     5281 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa 
     5341 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa 
     5401 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa 
     5461 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt 
     5521 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt 
     5581 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg 
     5641 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 
     5701 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 
     5761 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 
     5821 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 
     5881 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 
     5941 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 
     6001 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 
     6061 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 
     6121 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 
     6181 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 
     6241 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 
     6301 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 
     6361 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag 
     6421 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg 
     6481 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga 
     6541 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat 
     6601 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag 
     6661 ctatgaccat gattacgcca gatttaatta agg 
//
