LOCUS       48673\M-NM-sgRNA        3145 bp    DNA     circular     26-SEP-2013
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|674127681|
COMMENT     VNTDBDATE|674127681|
COMMENT     LSOWNER|
COMMENT     VNTNAME|48673 M-NM-sgRNA|
COMMENT     VNTAUTHORNAME|Kevin Esvelt|
COMMENT     VNTAUTHOREML|kesvelt@gmail.com|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     rep_origin      1315..1982
                     /vntifkey="33"
                     /label=pUC\origin
     CDS             complement(2130..2945)
                     /vntifkey="4"
                     /label=KanR
     promoter        432..749
                     /vntifkey="29"
                     /label=U6\promoter
     misc_feature    750..769
                     /vntifkey="21"
                     /label=spacer
     misc_feature    432..911
                     /vntifkey="21"
                     /label=U6\promoter\-\NMm1\sgRNA\(orientation\unknown)
     misc_feature    750..877
                     /vntifkey="21"
                     /label=NMm1\sgRNA
BASE COUNT      851 a       751 c       746 g       797 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa 
      421 tgcatctaga ttgtacaaaa aagcaggctt taaaggaacc aattcagtcg actggatccg 
      481 gtaccaaggt cgggcaggaa gagggcctat ttcccatgat tccttcatat ttgcatatac 
      541 gatacaaggc tgttagagag ataattagaa ttaatttgac tgtaaacaca aagatattag 
      601 tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat 
      661 gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt 
      721 tatatatctt gtggaaagga cgaaacaccg tcccctccac cccacagtgg ttgtagctcc 
      781 ctttctcgaa agagaaccgt tgctacaata aggccgtctg aaaagatgtg ccgcaacgct 
      841 ctgcccctta aagcttctgc tttaacgggc tttttttcta gacccagctt tcttgtacaa 
      901 agttggcatt aatcggatcc cgggcccgtc gactgcagag gcctgcatgc aagcttggcg 
      961 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 
     1021 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 
     1081 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 
     1141 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 
     1201 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 
     1261 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 
     1321 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 
     1381 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 
     1441 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 
     1501 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 
     1561 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 
     1621 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 
     1681 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 
     1741 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 
     1801 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 
     1861 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 
     1921 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 
     1981 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 
     2041 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 
     2101 agtatatatg agtaaacttg gtctgacagt tagaaaaact catcgagcat caaatgaaac 
     2161 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat 
     2221 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg 
     2281 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta 
     2341 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc 
     2401 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca 
     2461 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg 
     2521 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca 
     2581 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttccca 
     2641 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc 
     2701 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg 
     2761 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat 
     2821 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa 
     2881 tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg 
     2941 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 
     3001 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 
     3061 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat 
     3121 aggcgtatca cgaggccctt tcgtc 
//
