LOCUS pEMS1306 16454 bp DNA circular 16-FEB-2010 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|546089199| COMMENT VNTDBDATE|546089199| COMMENT LSOWNER| COMMENT VNTNAME|pEMS1306| COMMENT VNTAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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"Tetracysteine residue" "@N" 1 12.1782 COMMENT 13.4572 0 -10 10.0405 1.17389 #152#) COMMENT #154=(CScratch COMMENT (CWidget 0 (3 #11# 0) 1 2 0 0 #104# 718 100) COMMENT (LOGPEN 0 8 13395507) 10 1 5.91137 1.9 COMMENT 0.082322 1) COMMENT #155=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #96# 46454092 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "NLS" "@N" 1 COMMENT 9.15769 11.6649 0 -10 1.99811 1.17389 #154#) COMMENT #156=(CScratch COMMENT (CWidget 0 (3 #12# 0) 1 2 0 0 #104# 652 100) COMMENT (LOGPEN 0 8 13395507) 10 1 5.88164 1.9 COMMENT 0.082322 1) COMMENT #157=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #96# 46583052 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "MFRT" "@N" 1 COMMENT 11.3371 8.72369 0 -10 2.72243 1.17389 #156#) COMMENT #158=(CScratch COMMENT (CWidget 0 (3 #13# 0) 1 2 0 0 #104# 652 100) COMMENT (LOGPEN 0 8 13395507) 10 1 5.81034 1.9 COMMENT 0.082322 1) COMMENT #159=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #96# 35753148 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "SV40 early polyA signal" "@N" 1 15.5082 COMMENT 6.05723 0 -10 11.0645 1.17389 #158#)) COMMENT (CObjectList)) COMMENT #160=(CGroupWidget COMMENT (CWidget 22 (7 22 0) 1 2 0 0 Nil -251 100) COMMENT (CObjectList COMMENT #161=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #1# 0) 1 2 0 0 #112# 0 100) COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 0.835356 COMMENT 0.00228754 0.468182 1 0.082322) 0.8 1.8 1) COMMENT #162=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #96# 4063712 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "AmpR" "@N" 1 COMMENT -7.37024 11.5469 0 -10 2.87229 1.17389 #161#) COMMENT #163=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #2# 0) 1 2 0 0 #112# 0 100) COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 0.835356 COMMENT 2.34968 2.89335 1 0.082322) 0.8 1.8 0) COMMENT #164=(CLabel (CWidget 0 (0 0) 1 2 0 0 #96# 6 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "AmpR" "@N" 1 COMMENT -7.89797 -10.454 0 -10 2.87229 1.17389 #163#)) COMMENT (CObjectList)) COMMENT #165=(CGroupWidget COMMENT (CWidget 86 (7 86 0) 1 2 0 0 Nil -321 100) COMMENT (CObjectList COMMENT #166=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #0# 0) 1 2 0 0 #100# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.835356 0.886801 COMMENT 2.34015 1 0.082322) 0.8 1.8 0) COMMENT #167=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #96# 21894764 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "5' homology sequence" "@N" 1 -16.899 1.44863 COMMENT 0 -10 10.4901 1.17389 #166#) COMMENT #168=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #3# 0) 1 2 0 0 #100# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 2.89145 4.2815 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #169=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #96# 4063768 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "3' homology sequence" "@N" 1 11.0558 -10.454 COMMENT 0 -10 10.4901 1.17389 #168#) COMMENT #170=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #4# 0) 1 2 0 0 #100# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.835356 4.2815 COMMENT 5.78899 1 0.082322) 0.8 1.8 0) COMMENT #171=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #96# 22038808 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "HPRT complementary sequence" "@N" 1 18.381 COMMENT 1.74233 0 -10 14.9609 1.17389 #170#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #172=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -1023 100) COMMENT (CObjectList) (CObjectList)) COMMENT #173=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -1371 100) COMMENT (CObjectList) (CObjectList)) COMMENT #174=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -953 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 1699618931 COMMENT (CStringList) 0 0 (CObList))) FEATURES Location/Qualifiers misc_recomb 10317..14128 /vntifkey="86" /label=5'\homology\sequence misc_marker complement(15227..16448) /vntifkey="22" /label=AmpR misc_marker 8866..10291 /vntifkey="22" /label=AmpR misc_recomb 5225..8870 /vntifkey="86" /label=3'\homology\sequence /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3" misc_recomb 1271..5224 /vntifkey="86" /label=HPRT\complementary\sequence /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2" misc_feature 10298..10315 /vntifkey="21" /label=I-Sce\I misc_feature 122..152 /vntifkey="21" /label=MCS misc_feature 153..186 /vntifkey="21" /label=MFRT\(F5mutant) misc_feature 187..197 /vntifkey="21" /label=Kozak\consensus misc_feature 194..913 /vntifkey="21" /label=EGFP misc_feature 914..949 /vntifkey="21" /label=Tetracysteine\residue misc_feature 950..1022 /vntifkey="21" /label=NLS misc_feature 1028..1061 /vntifkey="21" /label=MFRT misc_feature 1215..1265 /vntifkey="21" /label=SV40\early\polyA\signal BASE COUNT 4417 a 3680 c 3671 g 4686 t ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctccta ggccggccac gcgtggcgcg ccgaagttcc tattcttcaa aaggtatagg 181 aacttccgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct 241 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg 301 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt 361 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc 421 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga 481 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga 541 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa 601 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga 661 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag 721 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct 781 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg 841 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga 901 gctgtacaag ttatttctga actgctgccc gggctgctgc atggaaccgg atccaaaaaa 961 gaagagaaag gtagatccaa aaaagaagag aaaggtagat ccaaaaaaga agagaaaggt 1021 aatataagaa gttcctattc tctagaaagt ataggaactt cagcggccgc gactctagat 1081 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct 1141 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc 1201 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 1261 actgcgaatt ctcgtaaaac tcttcatggc agtagttatt attctctctc tctctctttt 1321 tcttttttct tgagacagga tatttctctg ttgcccaggc tggagtgcag tggcacagtc 1381 ttggctcact gcagcctgga cctcctgggc tcaagccatc ctcccacctc agcctcccaa 1441 gtagctgggg ctacaggcac atggccacca ggcccagata atttttcatt tttgtagaga 1501 ctgagtctca ccatgttacc caggtttatt attctcattt tttagatgaa gagactgagg 1561 tccagagaag ctcaatgact tgcctagttt tacaaatctc ctgccatcac atacccctca 1621 gcgtccttaa taagagggag gccaccaact atgtgctggg cactgtggtg gatgctggag 1681 ctatagggtt gagtatataa gaaatggtgt tgctggagca actgttgctt gcttacctga 1741 cctatctgag aattaattag caggggaaca tatttttgtt ttcagattca atataagaac 1801 ttgtgtgggc aaaaataaag atcagtagta ataacagtag ttcccatttg ctgactgtac 1861 tgtcctaagt gcatatatat atacatacac acacgcatac ctatactcct ctaatactca 1921 aaatgatcct gtttatgtat tgttaatatg ctcattttat ttttaaattt ttatttattt 1981 ttatttttat ttatttttga gacggagtct cattctgtcg cggaggctga agtgcagtgg 2041 tgcgatctca gctcagtgcg acctccgcct cccgggttca agtgattctc ctgcctcagc 2101 ctccggacta gctgggatta caggcgcccg cctccacgcc cagctaattt ttgtattttt 2161 agtagagatg gggtttcgcc atgttggcca ggctggtctc gtactcctga ccttgagtga 2221 tccacctgcc tcggcctccc aaagtgctgg gattacaggc atgagccacc gcgccgggct 2281 aatatgctca ttttagtgag gcaaaaatag aggctcagag tctgatttgt acaaaactac 2341 agagcagtta agtgtcctct cagatgtgta ccctgatctg ggtgactcta ggactctagg 2401 tctcaactgt tacaaccagt taagggtttg gggaagcact gggccaagag tcaggaaaat 2461 ggaagccaca ggtagtgcaa ggtcttggga atgggacgtc tggtccaagg attcacgcga 2521 tgactggaac ccgaagagcc ggggcccggt ttacggccgc catgaagcaa cgcgcgccgg 2581 taggtttggg aatcagggag ccctctgaat aggagactga gttgggaggg aaaggggctt 2641 cgctggggga gcctcggctt cttctgggag aaaattccca cggctaccta gtgagcctgc 2701 aaactggtag gcgccggcgt aggcgcgcgg gcggggccgg gggcggggcc tgcggggcgt 2761 ggcggggcgg gcagagggcg gggcctgctt ctcctcagct tcaggcggct gcgacgagcc 2821 ctcaggcgaa cctctcggct ttcccgcgcg gcgccgcctc ttgctgcgcc tccgcctcct 2881 cctctgctcc gccaccggct tcctcctcct gagcagtcag cccgcgcgcc ggccggctcc 2941 gttatggcga cccgcagccc tggcgtcgtg gtgagcagct cggcctgccg gccctggccg 3001 gttcaggccc acgcggcagg tggcggccgg gccctgaggc gcgggatcga tccatgacac 3061 ccccccgccc cccatatctc actataattc actgtcaaat ggcttttatg atttggagtt 3121 tggggatact tttaaagaca gggtcttggt agctcctaga tggcttggga ttcacttagt 3181 aacccttgct ggcctaaaat tcaggaactt ctacctcatc ttcccaagta ctgggttagt 3241 agggatgtcc agcatattta gcttgcttta atgattttgc tgtacttttt tctgtgtgta 3301 caggtgcatg tatatttata aaactaacat aatatatttt acccccaaag taccataacc 3361 ctaattaatg ctattaaatg ttttgtatgc tgggctctgg gcatactaat ataaccaata 3421 ttatacaatg caaaacaatt ttggaatatc ttctgtaaaa ttatcttatt agtaagttgt 3481 aaccatctca gacttctaag tttgagtttg taaaggatca gttttttttg gtgacctaca 3541 cacacacaca cacacacaca cacacacaca cacacagggg ggtggattct gagaatttag 3601 agaatttaga tggttaatac cagtttttat ttttattctg tgtgatattt atttattaag 3661 atttatttat ttattacatg taggtacact gtatctgtct tcagacacac cagaagaggg 3721 catcagatct cgttacagat ggttgtgagt caccatgtgg ttgctgggat ttgaactcag 3781 gacctctgga agaacagtca gtgctcttaa ccgttgagcc atctcaccag ccccgtgata 3841 ttttatttaa cttactagaa tatactgtgt taaaacatat tttaggggat tggggagatg 3901 gcccattctg taaagtgtct gccatgtcat cttcaggacc tgcgtttgta accccagcat 3961 ggagaagaca gagacaggga gacccctggg cttcctggca agctagacta gcaaatttgg 4021 ttaactaggt tcaatgagag actcagtctc aaaacataag aagtgaattt tttagaccca 4081 atttcaatct ctgaactcca tacgtatgca catgtacctg catataaata tatatacaca 4141 cccataaaca catgcacaca caaaaaatgg ttcaaaagtt cttaaaatgt ataagagtag 4201 aaagtgtaga gccgggagtg gtggcgcacg cctttaatcc cagcacttgg gagcagaggc 4261 aggtggattt ctgagttcga ggccagcctg gtctacagag tgagttccag gacagccaga 4321 gctatacaga gaaatcctgc ctcaaaaaac caaaaataaa gtgtatatac taatctttca 4381 tgcctaaaat atttattttt gccacatttt tcatacctct ttaatatttt tctttttctc 4441 tttgtgcttc aaaggaaatt ttgtgtcatg ttttactttt cattcttgac tctattcagc 4501 agtaagacgc agcatggctt tcttagagaa gtataatcta ttacaaaacg gtaacattta 4561 actgctctac ataccctctt gtagctaaat tcctctgtta aactaagact tattatctaa 4621 acgtaattaa atgttgatat cttataatgc tccactttga aacagctgtc tttcttttgc 4681 agattagcga tgatgaacca ggttatgacc tagatttgtt ttgtatacct aatcattatg 4741 ccgaggattt ggaaaaagtg tttattcctc atggactgat tatggacagg ttagtaagac 4801 ctcgattgaa gttattttta gagggtgtgt gtgtggtgtg tgtgtgtgtg tgtgtgtgtg 4861 tgtgtgtgtg tgtgtaagag agagaggaga ggcatgtgtg atatatatgt gaagtgtatt 4921 gcatgtacat ggaatccaga gaaggacatt aggtgtccta ctctgttgct ctcttattcc 4981 cttgaatcag tgtctcttgc tgaacctggg gcatacatac tgatttggct aggctgcctg 5041 gctagcaaga cccagggatc tgcttgtctc tgccccccag tgctgacgtt acaagcaggt 5101 gcaaccacat ctggttttta tatgggtact gggggatctg aacttagggc ctgatgcgtg 5161 cccaagtgct ctcacccgcc aagccacctc tccagccaaa gggttttttt tcttgtatta 5221 attctaactt agcaactgtt gagtagttga tgtttgaata taagctaagc caacttatta 5281 gaataaatgt tttctatttt ttacttgtat gtgtgagtgc ctgcatgtat gtatgtgaag 5341 catatgtatt ccaggatgtc agaattgtga tggattccct ggaactggag ttacagacca 5401 ttgtgaacca ctgtgtatgg gtgctgggaa ctgaacttta ggtcctctgc aaaaacagta 5461 aggatcttaa ctgataaact atctttctag ccctaaggct ttctactttt aaagttcgtt 5521 tctctttctc tctctctctc tctctctctc tctctctctc cctctccctc cctcctcctc 5581 ctcctcctct ttcccccctc tcgcctcctc tttctctctc ccctccctct ctctctctca 5641 cacacacaca cagacagaga gaggagagag aaaggaagcc ttactaatta attacctagt 5701 tttagacata actagtggga atccatagta agtaaatcaa cattcttgaa gagaggttag 5761 aagaattggc tctcgttgtc catcgaggct gtataagatt attcttctgt tttctcagct 5821 taccatacat attttctacg tagacaatac caattcaaat acactgtacg taaagtatat 5881 gcgcacacac acacacacac acacacacac acacacacaa gttacatatt gtcatatagt 5941 actaaaattt atggactttt tcatattaat acatctagag ctatcatgtt ttttatcttt 6001 taaaaattac tttacggggc tacagagatg tctcagcagt taggagcact gactgctctt 6061 ccagaggtcc tgagttcaat tcccagcaac cacatggtgg ctcacaacca tctgtaatgg 6121 gatctgatcc actcttatgg tgtgtctgaa gacagtgaca gtgtactcat atacatgaaa 6181 taaatcttta aaaaattact ttaccatcag ggtatatata tatatatata tgtgtgtgtg 6241 tgtgtgtgtg tgtatgaagt atttgctatt tgagagggtc tcactgttta gctcagtctg 6301 gcttggaacg ttctgtatag cccagactgg cctcaactca gcagtccccc tgaaaggctc 6361 tcatatgtgg agattacagg tatgtgctac tgtgcccagc cctacatgtt actttgtttt 6421 tgaaataggg tatcaaatag cttagactgg cttctaagtc agtgtgtagc taaggatggt 6481 ctgagcttct gaacctgcta cttcaactcc tggtgtgcca tcatggccag tttgcacagt 6541 tgtgagagta gaactcagga cctcctatgt gttactagac atgttctcta ccagctgagc 6601 tacatcccaa ttcgcctaca tttactttta ttttttaaaa gatttattta tgtatacagc 6661 actctgcctg catgtacatc tgcatgccag aagagggcac cagatctcat catacatagt 6721 tgtgagccac catgtggttg ctggggattg aactcagggc ctctggaaga gcagtcagtg 6781 ctcttaacct ctgagccatc tctgtagccc cctacattta ttttaaagat tataggctat 6841 tctattcata gatgtgccat aactgattta accagatctt cattattttg tacttagtag 6901 agtatattag agggaatgaa tcttaaatac acattttgac acttgctcag ctttagacaa 6961 gtaaaaatca ctggtcaagg aaatggtgct tagtggatgc ttctgaactg tcaaattgtc 7021 ctccaacaag ttacatcatt ttatacttcc aacgttatgt gagagttcat ttttctttag 7081 gctcttctag tattttatat ttgagaaata aaatttaacc aaaatttgcc aatctagtaa 7141 gcttgttttt atttgtatct ttaaatttta tgatagatgt tatagtgtac tctcctctcc 7201 cctcccctcc cctctcctcc tcagcaggat cttgttatgt agccctaagg gatctaatac 7261 ttactctgta gtccaggctg tccttcagct catgagacac accatcatgc ttggcatgtt 7321 tcttgttctg aaaggtatct ggttttaatt ttggttgagc tggtgtgttg gtgtatatct 7381 gtaatctcag cacaggcaag tagagacaag gattatgatt acagtccagc ctgggctatt 7441 tagagggatt ctctttaatc tttcctcatg ccccaaaatc ttacctttgg tatatgaaaa 7501 atagtctcca cttctgcaaa atattgcttt atgaagtaag aattaattcc cttcatagag 7561 acaaggaatg tgtcctgtaa aagtttaatg tgtaagaagt atttgttata aaagataaat 7621 attcagaatc ttctttttaa ttcctgattt tatttctata ggactgaaag acttgctcga 7681 gatgtcatga aggagatggg aggccatcac attgtggccc tctgtgtgct caaggggggc 7741 tataagttct ttgctgacct gctggattac attaaagcac tgaatagaaa tagtgataga 7801 tccattccta tgactgtaga ttttatcaga ctgaagagct actgtgtaag tataattaac 7861 ttataattaa aaaaataggg ccattctagt tttatctata ttttttttaa acttgtgcaa 7921 actatgctac ataatcaact tacttgatta tgtaatatat atagtaatag ttggaattac 7981 tattattaca tatttatttt tctgtgctgg ggtagaaccc agggaactaa gtactgtaat 8041 aaccgagctt cattcatcct agtcattttt aatttttttt tttttttttg ttttttcgag 8101 acaaggtttc tctgtgtagc cctggctgtc ctggaactca ctctgtagac caggctggcc 8161 tcgaactcag aaatctgcct gcctctgcct cccagtgctg agattaatga cgtgcaccac 8221 cactgcctgg cattttttta aagttttaat ttgagaccct ttctcactaa cttgctcaca 8281 ctatcctcga actcattacg tgggctggtc tagaaatttc agtcaacttg cctcagcttc 8341 tcaagtagct gggattacag gcaggtgcta ccacttctgg ccatgatcat tgttgttgtt 8401 gctattatta ttattattat ttcactgata cttcatatca caacacagaa tagtaactgt 8461 agttcatgac caacaaaaaa gttgtacagt ttctctgttg tcttattttg atctggattt 8521 tgatttgttt catggcactg ggcaggctaa tctcaaactg catggctcaa atagtccttc 8581 tgtcttaatc tcccatgttc tggaagtata ggtatcacta cacctagctg tattttcata 8641 ttttaaaaaa aacgcctaag aattgctatt gaatgaggcg gagagatggc tcagcagtca 8701 caagtacttg tagaggactg gggtttggtt actagcacct caaagatggc ttacaattat 8761 ctgtagctcc aattccagag gatctgtgtg gtaaagctgc atgtatatgg gcatgcaggt 8821 aaaactgcta aacatataaa aatagctcag tcttaagaga attggtcgac tttttcgccc 8881 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact 8941 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg 9001 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct 9061 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc 9121 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa 9181 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt 9241 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct 9301 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc 9361 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta 9421 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac 9481 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc 9541 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac 9601 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg 9661 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac 9721 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc 9781 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt 9841 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga 9901 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 9961 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 10021 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca 10081 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 10141 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 10201 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 10261 tgcttgcaaa caaaaaaacc accgctacca ggtcgagtag ggataacagg gtaatggatc 10321 ctcgttgggc acctgcactc atgtacgcag gcccacatat agatgtacac acgcatatca 10381 ttcaaaatag aaattattta tacaagtagt gcgttggaga gctggctcag gagttgttaa 10441 tgcttgctac ccttgcaaag gacccaagtt caatttccag cacctgtgtt gggcatctca 10501 caaccacatg taaatctagt ttcaggggat ttcacccctc ttctgaactc ttcaggcact 10561 tatacccaca aatggacatc tccacataga gacacatact tttaaaaaag taatctaggg 10621 tctagagaaa tggttataga ccacggactg ggatcagttc ccagaagcca catcctgtgg 10681 ctcacaattg cctgtgaatc aagttctaga tctggtggcc tcgtctagcc tgcagtcatc 10741 aacaaacaca tgcacacatc catacacaca taatttaaaa gaaagcgaaa gtaacctata 10801 atgctaaaca aagcacattc tcagtgtaga ctgcagtggt gatgaacctt gaaaatatat 10861 taatagaagc cagacacgta agtcacaaat ggtataactc aatttatatg aaattcccag 10921 agagtccaaa gttaaaaaaa acaaaaaggc agattattgg ttgtcagtag ctacagagat 10981 gggacattga agatattaat aggtataggg ttgaggattc atggcctttc cctcttccat 11041 gttagcatgt ctattgttgt taaccttatt caactcatgt tagggcaatc atattgatga 11101 gacttaatga gtatagcttg acattcctag aagacagagt ctcacatcag actctctgat 11161 cctctgactc ttagaatctt tcaacaccct cttctgaaat gatctctgag cctttggttg 11221 tgactgaatt atgagaaaga attttatgtt gaatgaattt tacttcagca tttttacaaa 11281 actgaagctg gggatggcgt ttcatgcgtg cagtccctac catttgccta taagcctgga 11341 ggaggcagaa gtagaattag gcttttcagg gacatcttca gctgcatcat gagcttgagg 11401 acaacttgag ctacataaga ttcccatccc cacccccaca cacacacaca catctagtat 11461 gtacctagta tgcagtcttt tcatatgttt aaaatgtaga aaggtatatg tgaagtctcc 11521 cttttcccca caacttagtc cctccaaagt attgcatcag tttccttgta catgatttta 11581 tgcatatata agatacacta tttctctctc tctctctctc cttccctcct tccctccaac 11641 ctctgggtat gagttgaggg ttatgtatgt ttcaagttgt aataagaatt gccactcaaa 11701 catttcatcc cattttaagt taagtttgtc tgattttcca ctttagaagg gcagccatga 11761 tggctcatgc ctataatccc atcctagcac ttgtaagcct aatacataag actggaggat 11821 tgtcatcagc ttgaggctag cctggtctac ctaatgagtt ccagccagcc aagagtagaa 11881 tataagaccc tgtctccaaa aaatgtacca aaggggctgg aggggtaact cagtggttaa 11941 gaacagtagc tgctcttcca gaggacctgg gttcaattcc cagcacccac atggcagctt 12001 gcaactatct gtaactgtcc agctttggga tatccgacac cattatacca attacataaa 12061 atgaaaaaat tgtaaaatgt gctaaaatga gtggggaggg ggagcatggt gtggttctgt 12121 gggcttggga ttttgccatg aatccatgac tatatctcag caatatgtgg attatttctg 12181 gaaacctgtg gggcatgggt gtatgggaag aggggagaga gagagagccc gcatccagcc 12241 agagttcctg tgctctgggc aggcggacgt gggaggactg ctggacgatt tccacttggc 12301 cctgggtggg catctggctg tgtgaagcca ctgaccccac tcagcggggg gtagataagg 12361 ggcatttccc ataccaggtt ctcagactcc agcattccac gctggataca gcagaagagc 12421 tgagaacaga ataggggccc cagggagaca ctctcgtccc agagataaag aaggcagagg 12481 gagagagatt cctacgcagg cgagaggcct tagtctgggc cttgatgaac agagacggtc 12541 tatggtttta gagctttatt gtagaaaggc agggggaaag agagaaggta gaaagagaga 12601 gaccagccat ggccaagagg agagaagggg ggaaaggaga gagagaagaa aggctagaga 12661 gtaagaaagg ttaaagagac agaagtaaaa gcttagagta agagagagag agagagcaag 12721 agggagagta agaaaagtaa aagcttaaga gagcgaggag gggccagccc ctctaatagt 12781 gggcttgtta tcttgttgtt gctaggtaac tgtgaagcgt ctagcctgaa ggccagaagc 12841 ttgggacatt gtctatgtga ctgaaagcca tgagcctcta ctgtgggggc tgtggaggtg 12901 gtaacttgga caggagccag gggttccagg agacatgagg gaatgccttc tgtcccatgt 12961 aggtaggaat taccaccacc ggctttcacc gtagttcact ggagactgtt tgtacatagc 13021 ccattgcccc acagaaacca ggcactcact gtttcatttg cataagaatt agaaggcttt 13081 attttggctg ggattggtga cttgccccag aaattccagc ttttgggaag ccgaatcctg 13141 gagaaccagg agttcaaggt catcctcagg tacctcattt gttcaagacc agcctgtgct 13201 accatgagac cttgtcccta ctcccctcaa ccccaaaaca cgctgttttc attttatagg 13261 ttgtactaac catggaggat ggtcatttgc ctttctcttg atttcagaag cgtagcgcgg 13321 gctttgcacc atgatctagt gtttccagat aggcttgcct ctccacagcc aggcttatcc 13381 tgcttcagtc ccatgtttgg caagggcaac atatgactga agaacttgca tagccttatg 13441 gtgtatcatg actcttagcc ccagtttata actgtctaac agctggtggc ttttgtgaaa 13501 aaggaaagca tcatttgaac ttttaacttt tcagctgttt gttttgactt tgcataaata 13561 ctgagactag ctaggaaatg agcttttgtt tcatttgcta aaaatgattt ttttttcatg 13621 aggcaatttc aagcaatttg aatttagcaa tctctacttg tctgaaaaga agttattaat 13681 taaaaaaaaa caaacaaaca ggaacctatt atgctggcta gtcacatgaa agaatccaaa 13741 tgaacacagt caaggctgaa tcaacacatg aaattatttt ctaattcccc aaattacatt 13801 tatgctaatt agtatgtagt aaaacatttt aattgtttcc aatgttaaac attacagaaa 13861 taattttgat gttttcctaa taacccagcc tttgatcatg tttgcattta tttgattgtt 13921 gtcagtttat taaataatta agtaagagca acaagataaa atgacttcat gagagtcctt 13981 ttctgtaact tagaacgtca gtagtcatag gaactgcggt cgttccctta cctttcagaa 14041 gtaggctaaa gagttgaacg caaaggtggt ttttcgaatc ttcctgatcc ttaagccccc 14101 actaggttta tattctatat ttgatatcgg attcgaaccc ctcgagctta ggatccggaa 14161 cccttaatat aactaagtta ttaggtccct cgaagaggtt cactagtact ggccattgcg 14221 gccgcttcga ggctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca 14281 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca 14341 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga 14401 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac 14461 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct 14521 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 14581 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 14641 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 14701 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 14761 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 14821 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 14881 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 14941 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 15001 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 15061 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 15121 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc 15181 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 15241 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 15301 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 15361 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 15421 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 15481 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 15541 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 15601 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 15661 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 15721 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc 15781 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 15841 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 15901 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 15961 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 16021 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg 16081 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 16141 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 16201 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 16261 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 16321 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 16381 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 16441 gtgccacctg acgg //