LOCUS pCOCK-CMV-WT 6184 bp DNA circular 11-APR-2007 DEFINITION attB1-attB2. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|443404350| COMMENT VNTDBDATE|443404350| COMMENT LSOWNER| COMMENT VNTNAME|pCOCK-CMV-WT| COMMENT VNTAUTHORNAME|Demo User| FEATURES Location/Qualifiers misc_feature 5561..5581 /vntifkey="21" /label=attB1 misc_feature complement(6177..13) /vntifkey="21" /label=attB2 CDS 451..1167 /vntifkey="4" /label=EGFP /note="Enhanced Green Fluorescent Protein" primer 1190..1207 /vntifkey="27" /label=BGH\rev\primer /note="BGH reverse priming site" polyA_signal 1196..1420 /vntifkey="25" /label=BGH\polyA /note="BGH polyA signal" misc_recomb 1703..1750 /vntifkey="86" /label=FRT /note="FRT site" CDS 1703..2778 /vntifkey="4" /label=HygroR\(no\ATG) /note="hygromycin resistance gene (no ATG)" polyA_signal 2910..3040 /vntifkey="25" /label=SV40\polyA /note="SV40 early polyA signal" rep_origin 3423..4096 /vntifkey="33" /label=ColE1\ori CDS complement(4244..5101) /vntifkey="4" /label=AmpR promoter complement(5102..5200) /vntifkey="30" /label=P\bla primer complement(117..136) /vntifkey="27" /label=T3 /note="T3" primer 67..86 /vntifkey="27" /label=T7 /note="T7 primer" intron 143..438 /vntifkey="15" /label=IVS /note="Synthetic Intron" promoter 5585..6173 /vntifkey="29" /label=P\CMV /note="Human cytomegalovirus (CMV) immediate early promoter" BASE COUNT 1441 a 1639 c 1618 g 1486 t ORIGIN 1 ttgtacaaag tggttgatat ccagcacagt ggcggccgct cgagtctaga gggcccgcgg 61 ttcgaataat acgactcact atagggattt aaatttaatt aacctgcagg cttagctccc 121 tttagtgagg gttaatgtgc tggaattaat tcgctgtctg cgagggccag ctgttggggt 181 gagtactccc tctcaaaagc gggcatgact tctgcgctaa gattgtcagt ttccaaaaac 241 gaggaggatt tgatattcac ctggcccgcg gtgatgcctt tgagggtggc cgcgtccatc 301 tggtcagaaa agacaatctt tttgttgtca agcttgaggt gtggcaggct tgagatctgg 361 ccatacactt gagtgacaat gacatccact ttgcctttct ctccacaggt gtccactccc 421 aggtccaact gcaggtcgag catcgccacc atggtgagca agggcgagga gctgttcacc 481 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg 541 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc 601 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag 661 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc 721 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc 781 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac 841 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac 901 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac 961 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc 1021 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa 1081 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc 1141 actctcggca tggacgagct gtacaagtaa gtttaaaccc gctgatcagc ctcgactgtg 1201 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa 1261 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt 1321 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa 1381 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc 1441 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt 1501 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 1561 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 1621 gggtcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt 1681 agggtgatgg ttcacgtacc tagaagttcc tattccgaag ttcctattct ctagaaagta 1741 taggaacttc cttggccaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga 1801 tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg 1861 ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg 1921 gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg 1981 aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac 2041 agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg 2101 cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat 2161 tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg 2221 atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc 2281 aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc 2341 acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg 2401 actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga 2461 ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc 2521 ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc 2581 agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa 2641 tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg 2701 tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc 2761 gtccgagggc aaaggaatag cacgtactac gagatttcga ttccaccgcc gccttctatg 2821 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg 2881 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca 2941 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 3001 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct 3061 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 3121 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 3181 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 3241 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 3301 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 3361 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 3421 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 3481 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 3541 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 3601 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 3661 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 3721 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 3781 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 3841 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 3901 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 3961 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 4021 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 4081 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 4141 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 4201 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 4261 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 4321 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 4381 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 4441 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 4501 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 4561 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 4621 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 4681 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 4741 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 4801 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 4861 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 4921 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 4981 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 5041 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 5101 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 5161 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 5221 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag 5281 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga 5341 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa 5401 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag 5461 atatacgcgt tgacattgat tattgactag gcttttgcaa aaagcttggt accgagctcg 5521 gatccactag tccagtgtgg tggaattctg cagatatcaa caagtttgta caaaaaagca 5581 ggcttagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt 5641 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 5701 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 5761 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 5821 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc 5881 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 5941 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac 6001 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc 6061 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc 6121 gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag atcacccagc 6181 tttc //