LOCUS              pmaxCloning        4610 bp ds-DNA   circular     16-JAN-2013
DEFINITION  pmaxCloning
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          join(1..947,2783..4610)
                     /organism="pmaxCloning"
                     /mol_type="other DNA"
                     /label=source:pmaxCloning
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     promoter        163..715
                     /label=CMV_immearly_promoter
                     /ApEinfo_fwdcolor=#ffaae6
                     /ApEinfo_revcolor=#ff389a
     misc_feature    218..505
                     /label=CAG_enhancer
                     /ApEinfo_fwdcolor=#33ff00
                     /ApEinfo_revcolor=#bfc4ff
     promoter        661..742
                     /label=CMV_promoter
                     /ApEinfo_fwdcolor=#ffaae6
                     /ApEinfo_revcolor=#ff389a
     source          join(960..1162,1164..1776,1778..1966,1968..2184,2186..2511,
                     2513..2770)
                     /organism="pLenti6/Ubc/V5-DEST Gateway (ViraPower)"
                     /mol_type="other DNA"
                     /label=source:pLenti6/Ubc/V5-DEST Gateway (ViraPower)
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     misc_feature    672..692
                     /label=CMV_fwd_primer
                     /ApEinfo_fwdcolor=#33ff00
                     /ApEinfo_revcolor=#bfc4ff
     gene            join(1193..1776,1778..1852)
                     /gene="CAT/CamR"
                     /label=CAT/CamR
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     misc_feature    813..945
                     /label=chim_int
                     /ApEinfo_fwdcolor=#33ff00
                     /ApEinfo_revcolor=#bfc4ff
     misc_feature    complement(2717..2736)
                     /label=AC_V5_R
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    complement(2784..2803)
                     /label=EBV_rev_primer
                     /ApEinfo_fwdcolor=#33ff00
                     /ApEinfo_revcolor=#bfc4ff
     misc_feature    2194..2499
                     /label=ccdB
                     /ApEinfo_fwdcolor=#ffaa89
                     /ApEinfo_revcolor=#deff79
     rep_origin      complement(3077..3695)
                     /label=pBR322_origin
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     CDS             complement(3760..4605)
                     /translation="MGGDRDETNPDKCFNNIMIEQDGLHAGSPAAWVERLFGYDWAQQ
                     TIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVT
                     EAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIER
                     ARTRMEAGLVDQDDLDEEHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENG
                     RFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRL
                     LDEFF*"
                     /label=ORF frame 3
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     gene            complement(3763..4542)
                     /gene="NeoR/KanR (variant)"
                     /translation="MGGDRDETNPDKCFNNIMIEQDGLHAGSPAAWVERLFGYDWAQQ
                     TIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVT
                     EAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIER
                     ARTRMEAGLVDQDDLDEEHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENG
                     RFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRL
                     LDEFF*"
                     /label=NeoR/KanR (variant)
                     /ApEinfo_fwdcolor=#0004ff
                     /ApEinfo_revcolor=#ff00a8
     misc_feature    960..1084
                     /label=attR1
                     /ApEinfo_fwdcolor=#ffaa89
                     /ApEinfo_revcolor=#deff79
     misc_feature    complement(984..1084)
                     /label=attR2
                     /ApEinfo_fwdcolor=#ffaa89
                     /ApEinfo_revcolor=#deff79
     promoter        1109..1132
                     /label=lac_promoter
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     misc_feature    complement(join(1966..1966,1968..1987))
                     /label=AC_S104:hUbcAttRBGHPolyARSeq
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     CDS             join(1193..1776,1778..1852)
                     /gene="CAT/CamR"
                     /note="ORF frame 2"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
                     /label=CAT/CamR(1)
                     /ApEinfo_label=CAT/CamR
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     misc_feature    1163..1163
                     /label=pLentiSrcReseq.G->T
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    1569..1588
                     /label=AC_S97:CamRFSeq1
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    2377..2396
                     /label=AC_S105:hUbcAttRBGHPolyARSeq
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    1777..1777
                     /label=pLentiSrcReSeqC->TSymInCamR
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    2540..2640
                     /label=attR2(1)
                     /ApEinfo_label=attR2
                     /ApEinfo_fwdcolor=#ffaa89
                     /ApEinfo_revcolor=#deff79
     misc_feature    2228..2247
                     /label=AC_S98:ccdbFSeq
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    complement(2540..2647)
                     /label=attR1(1)
                     /ApEinfo_label=attR1
                     /ApEinfo_fwdcolor=#ffaa89
                     /ApEinfo_revcolor=#deff79
     misc_feature    2717..2758
                     /label=V5
                     /ApEinfo_fwdcolor=#ffaa89
                     /ApEinfo_revcolor=#deff79
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagg
      721 tcgtttagtg aaccgtcaga tcactagtag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctc gactgatcac aggtaagtat caaggttaca agacaggttt
      841 aaggaggcca atagaaactg ggcttgtcga gacagagaag attcttgcgt ttctgatagg
      901 cacctattgg tcttactgac atccactttg cctttctctc cacagggaaa aaaCAATTGA
      961 CAAGTTTGTA CAAAAAAGCT GAACGAGAAA CGTAAAATGA TATAAATATC AATATATTAA
     1021 ATTAGATTTT GCATAAAAAA CAGACTACAT AATACTGTAA AACACAACAT ATCCAGTCAC
     1081 TATGGCGGCC GCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATAATGT
     1141 GTGGATTTTG AGTTAGGATC CGTCGAGATT TTCAGGAGCT AAGGAAGCTA AAATGGAGAA
     1201 AAAAATCACT GGATATACCA CCGTTGATAT ATCCCAATGG CATCGTAAAG AACATTTTGA
     1261 GGCATTTCAG TCAGTTGCTC AATGTACCTA TAACCAGACC GTTCAGCTGG ATATTACGGC
     1321 CTTTTTAAAG ACCGTAAAGA AAAATAAGCA CAAGTTTTAT CCGGCCTTTA TTCACATTCT
     1381 TGCCCGCCTG ATGAATGCTC ATCCGGAATT CCGTATGGCA ATGAAAGACG GTGAGCTGGT
     1441 GATATGGGAT AGTGTTCACC CTTGTTACAC CGTTTTCCAT GAGCAAACTG AAACGTTTTC
     1501 ATCGCTCTGG AGTGAATACC ACGACGATTT CCGGCAGTTT CTACACATAT ATTCGCAAGA
     1561 TGTGGCGTGT TACGGTGAAA ACCTGGCCTA TTTCCCTAAA GGGTTTATTG AGAATATGTT
     1621 TTTCGTCTCA GCCAATCCCT GGGTGAGTTT CACCAGTTTT GATTTAAACG TGGCCAATAT
     1681 GGACAACTTC TTCGCCCCCG TTTTCACCAT GGGCAAATAT TATACGCAAG GCGACAAGGT
     1741 GCTGATGCCG CTGGCGATTC AGGTTCATCA TGCCGTTTGT GATGGCTTCC ATGTCGGCAG
     1801 AATGCTTAAT GAATTACAAC AGTACTGCGA TGAGTGGCAG GGCGGGGCGT AAAGATCTGG
     1861 ATCCGGCTTA CTAAAAGCCA GATAACAGTA TGCGTATTTG CGCGCTGATT TTTGCGGTAT
     1921 AAGAATATAT ACTGATATGT ATACCCGAAG TATGTCAAAA AGAGGTATGC TATGAAGCAG
     1981 CGTATTACAG TGACAGTTGA CAGCGACAGC TATCAGTTGC TCAAGGCATA TATGATGTCA
     2041 ATATCTCCGG TCTGGTAAGC ACAACCATGC AGAATGAAGC CCGTCGTCTG CGTGCCGAAC
     2101 GCTGGAAAGC GGAAAATCAG GAAGGGATGG CTGAGGTCGC CCGGTTTATT GAAATGAACG
     2161 GCTCTTTTGC TGACGAGAAC AGGGGCTGGT GAAATGCAGT TTAAGGTTTA CACCTATAAA
     2221 AGAGAGAGCC GTTATCGTCT GTTTGTGGAT GTACAGAGTG ATATTATTGA CACGCCCGGG
     2281 CGACGGATGG TGATCCCCCT GGCCAGTGCA CGTCTGCTGT CAGATAAAGT CTCCCGTGAA
     2341 CTTTACCCGG TGGTGCATAT CGGGGATGAA AGCTGGCGCA TGATGACCAC CGATATGGCC
     2401 AGTGTGCCGG TCTCCGTTAT CGGGGAAGAA GTGGCTGATC TCAGCCACCG CGAAAATGAC
     2461 ATCAAAAACG CCATTAACCT GATGTTCTGG GGAATATAAA TGTCAGGCTC CCTTATACAC
     2521 AGCCAGTCTG CAGGTCGACC ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT
     2581 CTGTTTTTTA TGCAAAATCT AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG
     2641 TTCAGCTTTC TTGTACAAAG TGGTTGATAT CCAGCACAGT GGCGGCCGCT CGAGTCTAGA
     2701 GGGCCCGCGG TTCGAAGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGCG
     2761 TACCGGTTAG CAATTGtttt ttcgatgagt ttggacaaac cacaactaga atgcagtgaa
     2821 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     2881 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     2941 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtacttaa gagggggaga
     3001 ccaaagggcg agacgttaag gcctcacgtg acatgtgagc aaaaggccag caaaaggcca
     3061 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     3121 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     3181 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     3241 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     3301 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     3361 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     3421 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     3481 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     3541 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     3601 ccggcaaaca aaccacgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     3661 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     3721 gaacgaaaac tcacgttaag ggattttggt catgccgtct cagaagaact cgtcaagaag
     3781 gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg
     3841 gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg
     3901 atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc
     3961 caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg
     4021 catgctcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc
     4081 cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg
     4141 tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc
     4201 atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc
     4261 cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc
     4321 tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cttgcagttc
     4381 attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag
     4441 ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag
     4501 cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcataatatt
     4561 attgaagcat ttatcagggt tcgtctcgtc ccggtctcct cccatgcatg 
//
