LOCUS       pAC-12-QFBDAD-G4MD50-761                  9424 bp ds-DNA     circular SYN 16-Jul-2004
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      Olena Riabinina, Potter Lab, Johns Hopkins University School of
            Medicine
FEATURES             Location/Qualifiers
     CDS             201..1058
                     /note=Amp
     promoter        2240..4793
                     /note=actin5c_promoter
     misc_feature    4799..4802
                     /note=Kozak
     gene            join(4803..5144,7278..7781)
                     /note=QFrco
     gene            5145..7277
                     /note=GAL4
     polyA_site      7788..9002
                     /note=actin5c_polyA
BASE COUNT      2685 A      2223 C      2049 G      2467 T         0 OTHER
ORIGIN      ?
        1 GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT
       61 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT
      121 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT
      181 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT
      241 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG
      301 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA
      361 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC
      421 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC
      481 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG
      541 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA
      601 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG
      661 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG
      721 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG
      781 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG
      841 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG
      901 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT
      961 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC
     1021 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT
     1081 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA
     1141 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT
     1201 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT
     1261 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC
     1321 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC
     1381 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC
     1441 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG
     1501 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT
     1561 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG
     1621 AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG
     1681 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT
     1741 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG
     1801 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT
     1861 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA
     1921 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT
     1981 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC
     2041 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA
     2101 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC
     2161 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG
     2221 ACCATGATTA CGAATTCTAT ATTCTAAAAA CACAAATGAT ACTTCTAAAA AAAAATCATG
     2281 AATGGCATCA ACTCTGAATC AAATCTTTGC AGATGCACCT ACTTCTCATT TCCACTGTCA
     2341 CATCATTTTT CCAGATCTCG CTGCCTGTTA TGTGGCCCAC AAACCAAGAC ACGTTTTATG
     2401 GCCATTAAAG CTGGCTGATC GTCGCCAAAC ACCAAATACA TAATGAATAT GTACACATTC
     2461 GAGAAAGAAG CGATCAAAGA AGCGTCTTCG GGCGGAGTAG GAGAATGCGG AGGAGAAGGA
     2521 GAACGAGCTG ATCTAGTATC TCTCCACAAT CCAATGCCAA CTGACCAACT GGCCATATTC
     2581 GGAGCAATTT GAAGCCAATT TCCATCGCCT GGCGATCGCT CCATTCTTGG CTATATGTTT
     2641 TTCACCGTTA CCCGGGGCCA TTTTCAAAGA CTCGTCGGCA AGATAAGATT GTGTCACTCG
     2701 CTGTCTCTCT TCATTTGTCG AAGAATGCTG AGGAATTTCG CGATGACGTC GGCGAGTATT
     2761 TTGAAGAATG AGAATAATTT GTATTTATAC GAAAATCAGT TAGTGGAATT TTCTACAAAA
     2821 ACATGTTATC TATAGATAAT TTTGTTGCAA AATATGTTGA CTATGACAAA GATTGTATGT
     2881 ATATACCTTT AATGTATTCT CATTTTCTTA TGTATTTATA ATGGCAATGA TGATACTGAT
     2941 GATATTTTAA GATGATGCCA GACCAAAAGG CTTGAATTTC TGCGTCTTTT GCCGAACGCA
     3001 GTGCATGTGC AATTGTTGTT TTTTGGAATA TTCAATTTTC GGACTGTCCG CTTTGATTTC
     3061 AGTTTCTTGG CTTATTCAAA AAGCAAAGTA AAGCCAAAAA AGCGAGATGG CAATACCAAA
     3121 TGCGGCAAAA CGGTAGTGGA AGGAAAGGGG TGCGGGGCAG CGGAAGGAAG GGTGGGGCGG
     3181 GGCGTGGCGG GGTCTGTGGC TGGGCGCGAC GTCACCGACG TTGGAGCCAC TCCTTTGACC
     3241 ATGTGTGCGT GTGTGTATTA TTCGTGTCTC GCCACTCGCC GGTTGTTTTT TTCTTTTTAT
     3301 GCTGCGCTCT CTCTAGCGCC ATCTCGCTTA CGCATGCTCA ACGCACCGCA TGTTGCCGTT
     3361 TCCTTTTATG CGTCATTTTG GCTCGAAATA GGCAATTATT TAAACAAAGA TTAGTCAACG
     3421 AAAACGCTAA AATAAATAAG TCTACAATAT GGTTACTTAT TGCCATGTGT GTGCAGCCAA
     3481 CGATAGCAAC AAAAGCAACA ACACAGGTGG CTTTCCCTCT TTCACTTTTT GTTTGCAAGC
     3541 CGCGTGCGAG CAAGACGGCA CGACCGGCAA ACGCAATTAC GCTGACAAAG AGCAGACGAA
     3601 GTTTTGGCGA AAAACATCAA GGCGCCTGAT ACGAATGCAT TTGCAATAAC AATTGCGATA
     3661 TTTAATATTG TTTATGAAGC TGTTTGACTT CAAAACACAC AAAAAAAAAA ATAAAACAAA
     3721 TTATTTGAAA GAGAATTAGG AATCGGACGC TTATCGTTAG GGTAACAACA AGAAATGCTT
     3781 ACTGAGTCAC AGCCTCTGGA AAACTGCCGC AAGCCAGAGA GAGAGAGAAA AAGAGGGAGA
     3841 GCAGCTTAGA CCGCATGTGC TTGTGTGTGA GGCGTCTCTC TCTTCGTCTC TGTTGCGCAA
     3901 ACGCATAGAC TGCACTGAAA AAATCGATTA CCTATTTTTT ATGAATGAAT ATTTGCACTA
     3961 TTACTATTCA AAACTATTAA GATAGCAATC ACATTCAATA GCCAAATACT ATACCACCTG
     4021 AGCGATGCAA CGAAATGATC AATTTGAGCA AAAATGCTGC ATATTTAGGA CGGCATCATT
     4081 ATAGAAATGC TTCTTGCTGT GTACTTTTCT CTCGTCTGGC AGCTGTTTCG CCGTTATTGT
     4141 TAAAACCGGC TTAAGTTAGG TGTGTTTTCT ACGACTAGCT AGTGAATGCC CTACTAGAAG
     4201 ATGTGTGTTG CACAAAATGT CCCTGGAATA ACCAATTTGA AGTGCAGATA GCAGTAAACG
     4261 TAAGCTAATA TGAATATTAT TTAACTGTAA TGTTTTAATA TCGCTGGACA TTACTAATAA
     4321 ACCCACTATA AACACATGTA CATATGTATG TTTTGGCATA CAATGAGTAG TTGGGGAAAA
     4381 AATGTGTAAA AGCACCGTGA CCATCACAGC ATAAAGATAA CCAGCTGAAG TATCGAATAT
     4441 GAGTAACCCC CAAATTGAAT CACATGCCGC AACTGATAGG ACCCATGGAA GTACACTCTT
     4501 CATGGCGATA TACAAGACAC ACACAAGCAC GAACACCCAG TTGCGGAGGA AATTCTCCGT
     4561 AAATGAAAAC CCAATCGGCG AACAATTCAT ACCCATATAT GGTAAAAGTT TTGAACGCGA
     4621 CTTGAGAGCG GAGAGCATTG CGGCTGATAA GGTTTTAGCG CTAAGCGGGC TTTATAAAAC
     4681 GGGCTGCGGG ACCAGTTTTC ATATCACTAC CGTTTGAGTT CTTGTGCTGT GTGGATACTC
     4741 CTCCCGACAC AAAGCCGCTC CATCAGCCAG CAGTCGTCTA ATCCAGAGAC CCCGGATCCA
     4801 ACATGCCACC CAAGCGCAAA ACGCTTAACG CTGCGGCTGA GGCTAACGCT CATGCCGACG
     4861 GACACGCCGA CGGAAACGCC GACGGACACG TGGCCAATAC GGCCGCGTCC TCGAATAATG
     4921 CGAGGTTCGC TGATCTCACT AACATCGATA CTCCGGGTCT GGGACCCACA ACTACGACCC
     4981 TGCTCGTGGA ACCAGCACGC TCAAAGCGTC AACGAGTGTC CCGCGCATGC GACCAGTGCC
     5041 GTGCAGCCCG AGAGAAATGC GACGGAATAC AGCCTGCGTG TTTCCCGTGC GTTTCCCAGG
     5101 GAAGGTCCTG CACTTATCAG GCTTCGCCGA AAAAGAGGGG AGTTACTAGG GCACATCTGA
     5161 CAGAAGTGGA ATCAAGGCTA GAAAGACTGG AACAGCTATT TCTACTGATT TTTCCTCGAG
     5221 AAGACCTTGA CATGATTTTG AAAATGGATT CTTTACAGGA TATAAAAGCA TTGTTAACAG
     5281 GATTATTTGT ACAAGATAAT GTGAATAAAG ATGCCGTCAC AGATAGATTG GCTTCAGTGG
     5341 AGACTGATAT GCCTCTAACA TTGAGACAGC ATAGAATAAG TGCGACATCA TCATCGGAAG
     5401 AGAGTAGTAA CAAAGGTCAA AGACAGTTGA CTGTATCGAT TGACTCGGCA GCTCATCATG
     5461 ATAACTCCAC AATTCCGTTG GATTTTATGC CCAGGGATGC TCTTCATGGA TTTGATTGGT
     5521 CTGAAGAGGA TGACATGTCG GATGGCTTGC CCTTCCTGAA AACGGACCCC AACAATAATG
     5581 GGTTCTTTGG CGACGGTTCT CTCTTATGTA TTCTTCGATC TATTGGCTTT AAACCGGAAA
     5641 ATTACACGAA CTCTAACGTT AACAGGCTCC CGACCATGAT TACGGATAGA TACACGTTGG
     5701 CTTCTAGATC CACAACATCC CGTTTACTTC AAAGTTATCT CAATAATTTT CACCCCTACT
     5761 GCCCTATCGT GCACTCACCG ACGCTAATGA TGTTGTATAA TAACCAGATT GAAATCGCGT
     5821 CGAAGGATCA ATGGCAAATC CTTTTTAACT GCATATTAGC CATTGGAGCC TGGTGTATAG
     5881 AGGGGGAATC TACTGATATA GATGTTTTTT ACTATCAAAA TGCTAAATCT CATTTGACGA
     5941 GCAAGGTCTT CGAGTCAGGT TCCATAATTT TGGTGACAGC CCTACATCTT CTGTCGCGAT
     6001 ATACACAGTG GAGGCAGAAA ACAAATACTA GCTATAATTT TCACAGCTTT TCCATAAGAA
     6061 TGGCCATATC ATTGGGCTTG AATAGGGACC TCCCCTCGTC CTTCAGTGAT AGCAGCATTC
     6121 TGGAACAAAG ACGCCGAATT TGGTGGTCTG TCTACTCTTG GGAGATCCAA TTGTCCCTGC
     6181 TTTATGGTCG ATCCATCCAG CTTTCTCAGA ATACAATCTC CTTCCCTTCT TCTGTCGACG
     6241 ATGTGCAGCG TACCACAACA GGTCCCACCA TATATCATGG CATCATTGAA ACAGCAAGGC
     6301 TCTTACAAGT TTTCACAAAA ATCTATGAAC TAGACAAAAC AGTAACTGCA GAAAAAAGTC
     6361 CTATATGTGC AAAAAAATGC TTGATGATTT GTAATGAGAT TGAGGAGGTT TCGAGACAGG
     6421 CACCAAAGTT TTTACAAATG GATATTTCCA CCACCGCTCT AACCAATTTG TTGAAGGAAC
     6481 ACCCTTGGCT ATCCTTTACA AGATTCGAAC TGAAGTGGAA ACAGTTGTCT CTTATCATTT
     6541 ATGTATTAAG AGATTTTTTC ACTAATTTTA CCCAGAAAAA GTCACAACTA GAACAGGATC
     6601 AAAATGATCA TCAAAGTTAT GAAGTTAAAC GATGCTCCAT CATGTTAAGC GATGCAGCAC
     6661 AAAGAACTGT TATGTCTGTA AGTAGCTATA TGGACAATCA TAATGTCACC CCATATTTTG
     6721 CCTGGAATTG TTCTTATTAC TTGTTCAATG CAGTCCTAGT ACCCATAAAG ACTCTACTCT
     6781 CAAACTCAAA ATCGAATGCT GAGAATAACG AGACCGCACA ATTATTACAA CAAATTAACA
     6841 CTGTTCTGAT GCTATTAAAA AAACTGGCCA CTTTTAAAAT CCAGACTTGT GAAAAATACA
     6901 TTCAAGTACT GGAAGAGGTA TGTGCGCCGT TTCTGTTATC ACAGTGTGCA ATCCCATTAC
     6961 CGCATATCAG TTATAACAAT AGTAATGGTA GCGCCATTAA AAATATTGTC GGTTCTGCAA
     7021 CTATCGCCCA ATACCCTACT CTTCCGGAGG AAAATGTCAA CAATATCAGT GTTAAATATG
     7081 TTTCTCCTGG CTCAGTAGGG CCTTCACCTG TGCCATTGAA ATCAGGAGCA AGTTTCAGTG
     7141 ATCTAGTCAA GCTGTTATCT AACCGTCCAC CCTCTCGTAA CTCTCCAGTG ACAATACCAA
     7201 GAAGCACACC TTCGCATCGC TCAGTCACGC CTTTTCTAGG GCAACAGCAA CAGCTGCAAT
     7261 CATTAGTGCC ACTGACCCGT CAGTTGGAGC TCCCTCCTAC CGCCACGGCT ACGGCCTCGA
     7321 TAATGCCGCA CGTGATGGAG CAGCCTCTCA GTACCAGCAT TAACCCCGTC AACGACCGCT
     7381 TCAACGGTAT TCCCAACCCC ACTCCGTATA ACTCCGATGC AGCTCTCGAT GCTATCACTC
     7441 AGACCAACGA TTATGGAAGC GTAAATACAC ATGGTATCCT CTCTACTTAC CCGCCACCGG
     7501 CTACGCACCT TAATGAAGCT TCCGTCGCTC TCGCTCCCGG TGGCGCCCCC CCCCGACCGC
     7561 CTCCTCCGTA TGTTGACAGC ACGACCAATC ACCCGCCGTA CCACTCGAAT CTGGTTCCAA
     7621 TGGCGAACTT TGGTTACTCG ACCGTTGATT ACGATGCCAT GGTTGACGAT TTGGCTAGCA
     7681 TTGAATACAC GGACGCTGTG GATGTCGACC CACAGTTTAT GACCAATCTG GGATTCGTTC
     7741 CTGGATGTAA CTTCTCCGAC ATTAATACAT ACGAACAGTG AGGCCGCGTT AACCGATCCG
     7801 TCGACCATGA AGATCAAGAT CATTGCCCCG CCAGAGCGCA AGTACTCTGT CTGGATCGGT
     7861 GGCTCCATCC TGGCTTCGCT GTCCACCTTC CAGCAGATGT GGATCTCCAA GCAGGAGTAC
     7921 GACGAGTCCG GCCCCTCCAT TGTGCACCGC AAGTGCTTCT AAGAAGGATC GCTTGTCTGG
     7981 GCAAGAGGAT CAGGATCGGG ATGGTCTTGA TTCTGCTGGA GGAGGAGGAG GAGAAGTCGA
     8041 GGAAGCAGCA GCGAAAGTGC AAGTGCGAGT GGTGGAAGTT TGGAGTGCAG CACAACAAAA
     8101 TCAACAACAA CACCAACTAC AAGATGAAAA GAGCGGAACC ACCTGCACAC CATCATCACT
     8161 ATCATCATCG TTTTGGGCGC ATGTTGTGTG GTTCCAGCGT ATTAATATAA TTAATTTATT
     8221 CCACATGAGA TATGATATGA TATACTATGT ATTTTTTGTT TTTTTTTTAT TTGTAAACCT
     8281 TTAATATAAC AAGAACTACA AAAAATGAAA ATGAGCGAAA ATGCATATTC TGCCATTCCA
     8341 CACACACACC AACAACACCC AACACACGCA CACCCACAAG CTTACACACA CACATTCGCG
     8401 GCATGACAAG GACATCAAGA TAAAGAAGAA CTTAAAGAAG ATATTTCCCA AAGCGCAAAA
     8461 AGAACACACA CACATTGCAA AACACAAACA ACACACTAGC GTTTTGTACA ATTCGTCAGC
     8521 AACCTTATGT ATTATTTTTT AATTATGATG TAATTATAAA CAAAGTGAAA CAAAAATATG
     8581 AAAACAAAAA GGAAAATCAA ATCTGTCTTC TCTTTCTCCC GCTCTCCTCG CTCTCTGCTG
     8641 CTAACCTCGC CCTCTCCTCT CTCATCTTTT TGTCTGTCTC TCTTCCACAT TTTTGCCGGC
     8701 CGGCAAAATA ATAACCCACA CACACTCACA CTTGGCTGCA GTTTCGCGTG CGATATTCAC
     8761 ACACATTCAA GCATACAAGC ATACATACAT ATGTATTTTT TTTTTATTTG TACACTTTTC
     8821 TAATTGCATG CGTATCGATT GATAAGTTTA CGCCTGAAAA TGTTAATTAA AATGTGAAAA
     8881 TGCAACTGAA AAACTGATGA AATGAAACAA CAACAAGCGA ACAATTTGCT ACATGTGTAT
     8941 TGTCTAACAA CCGTTACTGC AACGGTTGCT TCGGAAAAGG GTGAAGAGGA AGAGGGCACG
     9001 AGGTCGACCT GCAGGCATGC AAGCTTGGCA CTGGCCGTCG TTTTACAACG TCGTGACTGG
     9061 GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG
     9121 CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC
     9181 GAATGGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA
     9241 TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGCC CCGACACCCG
     9301 CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA
     9361 GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC
     9421 GCGA
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