LOCUS       Ple51\(pEMS1113)       18504 bp    DNA     circular     11-FEB-2010
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|545663686|
COMMENT     VNTDBDATE|545663686|
COMMENT     LSOWNER|
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COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 
COMMENT                "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 
COMMENT         (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 
COMMENT          (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 -365) 
COMMENT        1 1 0 1 1 
COMMENT        (mapper: 23.3883 -17.8935 40.0378 40.0378 0.01 10 -1 18504 18504 1 0 0) 
COMMENT        #141=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 1937009781 100) 
COMMENT              (CObjectList 
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COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 58 22 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 
COMMENT                            1.74233 0.833333 0 "Ple51 (pEMS1113)" "@N" 1 0 
COMMENT                            0.871165 0 -10 10.7149 1.47361 Nil) 
COMMENT                      #145=(CLabel (CWidget 1002 (0 0) 1 2 0 0 #103# 0 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 
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COMMENT               #146=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -1023 100) 
COMMENT                     (CObjectList 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
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COMMENT                                   "MFRT (F5 mutant)" "@N" 1 10.8144 13.1075 0 
COMMENT                                   -10 8.21723 1.17389 #148#) 
COMMENT                             #150=(CScratch 
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COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Kozak consensus sequence" "@N" 1 13.8794 
COMMENT                                   11.515 0 -10 12.7629 1.17389 #150#) 
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COMMENT                                   8.16397 9.59786 0 -10 2.67247 1.17389 #152#) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "NLS" "@N" 1 
COMMENT                                   15.5681 3.26589 0 -10 1.99811 1.17389 #156#) 
COMMENT                             #158=(CScratch 
COMMENT                                   (CWidget 0 (3 #6# 0) 1 2 0 0 #110# 929 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.169 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #159=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 early polyA signal" "@N" 1 16.7258 
COMMENT                                   -1.74233 0 -10 11.0645 1.17389 #158#) 
COMMENT                             #160=(CWideArrow 
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COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "HPRT complementary sequence" "@N" 1 18.2582 
COMMENT                                   -5.10813 0 -10 14.9609 1.17389 #160#) 
COMMENT                             #162=(CWideArrow 
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COMMENT                             #163=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                   "3' homology sequence" "@N" 1 5.94586 
COMMENT                                   -12.1963 0 -10 10.4901 1.17389 #162#) 
COMMENT                             #164=(CWideArrow 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "5' homology sequence" "@N" 1 -16.3888 
COMMENT                                   3.64054 0 -10 10.4901 1.17389 #164#) 
COMMENT                             #166=(CScratch 
COMMENT                                   (CWidget 0 (3 #12# 0) 1 2 0 0 #110# 845 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 2.08733 1.9 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "I-Sce I" "@N" 1 
COMMENT                                   -12.5319 -6.77553 0 -10 3.02214 1.17389 #166#) 
COMMENT                             #168=(CWideArrow 
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COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                                    6.2264 1 0.082322) 0.8 1.8 0) 
COMMENT                             #169=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                   "@N" 1 -0.77793 15.5681 0 -10 6.46889 1.17389 
COMMENT                                   #168#) 
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COMMENT                                   "@N" 1 8.54215 14.7 0 -10 6.16917 1.17389 
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COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #182=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -883 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #183=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -604 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 
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FEATURES             Location/Qualifiers
     misc_feature    1206..1239
                     /vntifkey="21"
                     /label=MFRT\(F5\mutant)
     misc_feature    1240..1251
                     /vntifkey="21"
                     /label=Kozak\consensus\sequence
     misc_feature    1252..1968
                     /vntifkey="21"
                     /label=EGFP
     misc_feature    1969..2994
                     /vntifkey="21"
                     /label=Cre
     misc_feature    2995..3067
                     /vntifkey="21"
                     /label=NLS
     misc_marker     3071..3104
                     /vntifkey="22"
                     /label=MFRT
     misc_feature    3258..3308
                     /vntifkey="21"
                     /label=SV40\early\polyA\signal
     misc_feature    3314..7267
                     /vntifkey="21"
                     /label=HPRT\complementary\sequence
                     /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2"
     misc_feature    7268..10913
                     /vntifkey="21"
                     /label=3'\homology\sequence
                     /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3"
     misc_feature    12367..16178
                     /vntifkey="21"
                     /label=5'\homology\sequence
     misc_marker     10915..12341
                     /vntifkey="22"
                     /label=AmpR
     misc_marker     complement(17498..18498)
                     /vntifkey="22"
                     /label=AmpR
     misc_feature    12348..12365
                     /vntifkey="21"
                     /label=I-Sce\I
     misc_feature    139..1197
                     /vntifkey="21"
                     /label=Ple51\(DBH-D)
                     /note="1302"
     misc_feature    936..1197
                     /vntifkey="21"
                     /label=Region\Prom
     misc_feature    139..935
                     /vntifkey="21"
                     /label=Region\3
BASE COUNT     4924 a      4156 c      4203 g      5221 t 
ORIGIN
        1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 
       61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 
      121 cgagctccta ggccggccgc tgtgctcagc taacagtcgc ctgtggtttt tttttttttt 
      181 tcctggctgc ataagtaata ccttccgcca ctgttggagt tatctccaca tgcacagaga 
      241 agggtataag tcagcttggg ccttagcatg acacgggatt tattgctcat ttaaaccctg 
      301 aaagcaaatg gatttttgaa aactcctgaa gcatcaggga agtctgtctc ttcatgatta 
      361 tctggttgta tttatggatc ctttgccaaa tataaggacg ctcctgtcac ttcacccatc 
      421 ttgagatggg ctgcttttct ataaatatca aaatcccaca attatttcat gatttaatcc 
      481 ccatctgaat tttaaccaat tgcaacaccc tttttattat tatcctctga tgtaatcagc 
      541 ctggagatac aggagccgtt tgagtttgga tggtgtaggg gtgtcgaggg aagaaggcag 
      601 aggcaagagg agttgttttg gcctcatctt aaaggaaaca agtagggaga tggttgggcc 
      661 ccctggaagt gccctgcacc ccagtccaca gccctgggtt tgaacctcag cttggccact 
      721 tcccagccct gtggtcttgt gtggagtctt cagttcaggc tccatcctca gtttcttaat 
      781 ctgtgaaata gggctgatga ttctcactgt gcaaaacttc catgaaaggg gtaggttatg 
      841 cttagagcat ttttggcacc cagggaagat gttagtatct gtaccaccct cttggtttga 
      901 tcagaaaggg caatgcatgt acactggtga gaagagctct tcagagccat ctcagaaggg 
      961 acagcatccg cctgtctact tcaactccca ctgatgacgt ccatgtgtca ttagtgccaa 
     1021 ttagaggagg gcagcaggct gagtgcttgg cctggggcgc aagcttgtgg gagggaaaat 
     1081 tggattcccc gctagacaaa tgtgattacc cgtgctgcct ggacccaccc cattcaggac 
     1141 cagggcataa atggccaggt gggaccagag agctcacccc agccatgccc gccctcaggc 
     1201 gcgccgaagt tcctattctt caaaaggtat aggaacttcg ccgccaccat ggtgagcaag 
     1261 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac 
     1321 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc 
     1381 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc 
     1441 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc 
     1501 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac 
     1561 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc 
     1621 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac 
     1681 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg 
     1741 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag 
     1801 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc 
     1861 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc 
     1921 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagttatc caatttactg 
     1981 accgtacacc aaaatttgcc tgcattaccg gtcgatgcaa cgagtgatga ggttcgcaag 
     2041 aacctgatgg acatgttcag ggatcgccag gcgttttctg agcatacctg gaaaatgctt 
     2101 ctgtccgttt gccggtcgtg ggcggcatgg tgcaagttga ataaccggaa atggtttccc 
     2161 gcagaacctg aagatgttcg cgattatctt ctatatcttc aggcgcgcgg tctggcagta 
     2221 aaaactatcc agcaacattt gggccagcta aacatgcttc atcgtcggtc cgggctgcca 
     2281 cgaccaagtg acagcaatgc tgtttcactg gttatgcggc ggatccgaaa agaaaacgtt 
     2341 gatgccggtg aacgtgcaaa acaggctcta gcgttcgaac gcactgattt cgaccaggtt 
     2401 cgttcactca tggaaaatag cgatcgctgc caggatatac gtaatctggc atttctgggg 
     2461 attgcttata acaccctgtt acgtatagcc gaaattgcca ggatcagggt taaagatatc 
     2521 tcacgtactg acggtgggag aatgttaatc catattggca gaacgaaaac gctggttagc 
     2581 accgcaggtg tagagaaggc acttagcctg ggggtaacta aactggtcga gcgatggatt 
     2641 tccgtctctg gtgtagctga tgatccgaat aactacctgt tttgccgggt cagaaaaaat 
     2701 ggtgttgccg cgccatctgc caccagccag ctatcaactc gcgccctgga agggattttt 
     2761 gaagcaactc atcgattgat ttacggcgct aaggatgact ctggtcagag atacctggcc 
     2821 tggtctggac acagtgcccg tgtcggagcc gcgcgagata tggcccgcgc tggagtttca 
     2881 ataccggaga tcatgcaagc tggtggctgg accaatgtaa atattgtcat gaactatatc 
     2941 cgtaacctgg atagtgaaac aggggcaatg gtgcgcctgc tggaagatgg cgatgatcca 
     3001 aaaaagaaga gaaaggtaga tccaaaaaag aagagaaagg tagatccaaa aaagaagaga 
     3061 aaggtaataa gaagttccta ttctctagaa agtataggaa cttcagcggc cgcgactcta 
     3121 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca 
     3181 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc 
     3241 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 
     3301 ttcactgcga attctcgtaa aactcttcat ggcagtagtt attattctct ctctctctct 
     3361 ttttcttttt tcttgagaca ggatatttct ctgttgccca ggctggagtg cagtggcaca 
     3421 gtcttggctc actgcagcct ggacctcctg ggctcaagcc atcctcccac ctcagcctcc 
     3481 caagtagctg gggctacagg cacatggcca ccaggcccag ataatttttc atttttgtag 
     3541 agactgagtc tcaccatgtt acccaggttt attattctca ttttttagat gaagagactg 
     3601 aggtccagag aagctcaatg acttgcctag ttttacaaat ctcctgccat cacatacccc 
     3661 tcagcgtcct taataagagg gaggccacca actatgtgct gggcactgtg gtggatgctg 
     3721 gagctatagg gttgagtata taagaaatgg tgttgctgga gcaactgttg cttgcttacc 
     3781 tgacctatct gagaattaat tagcagggga acatattttt gttttcagat tcaatataag 
     3841 aacttgtgtg ggcaaaaata aagatcagta gtaataacag tagttcccat ttgctgactg 
     3901 tactgtccta agtgcatata tatatacata cacacacgca tacctatact cctctaatac 
     3961 tcaaaatgat cctgtttatg tattgttaat atgctcattt tatttttaaa tttttattta 
     4021 tttttatttt tatttatttt tgagacggag tctcattctg tcgcggaggc tgaagtgcag 
     4081 tggtgcgatc tcagctcagt gcgacctccg cctcccgggt tcaagtgatt ctcctgcctc 
     4141 agcctccgga ctagctggga ttacaggcgc ccgcctccac gcccagctaa tttttgtatt 
     4201 tttagtagag atggggtttc gccatgttgg ccaggctggt ctcgtactcc tgaccttgag 
     4261 tgatccacct gcctcggcct cccaaagtgc tgggattaca ggcatgagcc accgcgccgg 
     4321 gctaatatgc tcattttagt gaggcaaaaa tagaggctca gagtctgatt tgtacaaaac 
     4381 tacagagcag ttaagtgtcc tctcagatgt gtaccctgat ctgggtgact ctaggactct 
     4441 aggtctcaac tgttacaacc agttaagggt ttggggaagc actgggccaa gagtcaggaa 
     4501 aatggaagcc acaggtagtg caaggtcttg ggaatgggac gtctggtcca aggattcacg 
     4561 cgatgactgg aacccgaaga gccggggccc ggtttacggc cgccatgaag caacgcgcgc 
     4621 cggtaggttt gggaatcagg gagccctctg aataggagac tgagttggga gggaaagggg 
     4681 cttcgctggg ggagcctcgg cttcttctgg gagaaaattc ccacggctac ctagtgagcc 
     4741 tgcaaactgg taggcgccgg cgtaggcgcg cgggcggggc cgggggcggg gcctgcgggg 
     4801 cgtggcgggg cgggcagagg gcggggcctg cttctcctca gcttcaggcg gctgcgacga 
     4861 gccctcaggc gaacctctcg gctttcccgc gcggcgccgc ctcttgctgc gcctccgcct 
     4921 cctcctctgc tccgccaccg gcttcctcct cctgagcagt cagcccgcgc gccggccggc 
     4981 tccgttatgg cgacccgcag ccctggcgtc gtggtgagca gctcggcctg ccggccctgg 
     5041 ccggttcagg cccacgcggc aggtggcggc cgggccctga ggcgcgggat cgatccatga 
     5101 cacccccccg ccccccatat ctcactataa ttcactgtca aatggctttt atgatttgga 
     5161 gtttggggat acttttaaag acagggtctt ggtagctcct agatggcttg ggattcactt 
     5221 agtaaccctt gctggcctaa aattcaggaa cttctacctc atcttcccaa gtactgggtt 
     5281 agtagggatg tccagcatat ttagcttgct ttaatgattt tgctgtactt ttttctgtgt 
     5341 gtacaggtgc atgtatattt ataaaactaa cataatatat tttaccccca aagtaccata 
     5401 accctaatta atgctattaa atgttttgta tgctgggctc tgggcatact aatataacca 
     5461 atattataca atgcaaaaca attttggaat atcttctgta aaattatctt attagtaagt 
     5521 tgtaaccatc tcagacttct aagtttgagt ttgtaaagga tcagtttttt ttggtgacct 
     5581 acacacacac acacacacac acacacacac acacacacag gggggtggat tctgagaatt 
     5641 tagagaattt agatggttaa taccagtttt tatttttatt ctgtgtgata tttatttatt 
     5701 aagatttatt tatttattac atgtaggtac actgtatctg tcttcagaca caccagaaga 
     5761 gggcatcaga tctcgttaca gatggttgtg agtcaccatg tggttgctgg gatttgaact 
     5821 caggacctct ggaagaacag tcagtgctct taaccgttga gccatctcac cagccccgtg 
     5881 atattttatt taacttacta gaatatactg tgttaaaaca tattttaggg gattggggag 
     5941 atggcccatt ctgtaaagtg tctgccatgt catcttcagg acctgcgttt gtaaccccag 
     6001 catggagaag acagagacag ggagacccct gggcttcctg gcaagctaga ctagcaaatt 
     6061 tggttaacta ggttcaatga gagactcagt ctcaaaacat aagaagtgaa ttttttagac 
     6121 ccaatttcaa tctctgaact ccatacgtat gcacatgtac ctgcatataa atatatatac 
     6181 acacccataa acacatgcac acacaaaaaa tggttcaaaa gttcttaaaa tgtataagag 
     6241 tagaaagtgt agagccggga gtggtggcgc acgcctttaa tcccagcact tgggagcaga 
     6301 ggcaggtgga tttctgagtt cgaggccagc ctggtctaca gagtgagttc caggacagcc 
     6361 agagctatac agagaaatcc tgcctcaaaa aaccaaaaat aaagtgtata tactaatctt 
     6421 tcatgcctaa aatatttatt tttgccacat ttttcatacc tctttaatat ttttcttttt 
     6481 ctctttgtgc ttcaaaggaa attttgtgtc atgttttact tttcattctt gactctattc 
     6541 agcagtaaga cgcagcatgg ctttcttaga gaagtataat ctattacaaa acggtaacat 
     6601 ttaactgctc tacataccct cttgtagcta aattcctctg ttaaactaag acttattatc 
     6661 taaacgtaat taaatgttga tatcttataa tgctccactt tgaaacagct gtctttcttt 
     6721 tgcagattag cgatgatgaa ccaggttatg acctagattt gttttgtata cctaatcatt 
     6781 atgccgagga tttggaaaaa gtgtttattc ctcatggact gattatggac aggttagtaa 
     6841 gacctcgatt gaagttattt ttagagggtg tgtgtgtggt gtgtgtgtgt gtgtgtgtgt 
     6901 gtgtgtgtgt gtgtgtgtaa gagagagagg agaggcatgt gtgatatata tgtgaagtgt 
     6961 attgcatgta catggaatcc agagaaggac attaggtgtc ctactctgtt gctctcttat 
     7021 tcccttgaat cagtgtctct tgctgaacct ggggcataca tactgatttg gctaggctgc 
     7081 ctggctagca agacccaggg atctgcttgt ctctgccccc cagtgctgac gttacaagca 
     7141 ggtgcaacca catctggttt ttatatgggt actgggggat ctgaacttag ggcctgatgc 
     7201 gtgcccaagt gctctcaccc gccaagccac ctctccagcc aaagggtttt ttttcttgta 
     7261 ttaattctaa cttagcaact gttgagtagt tgatgtttga atataagcta agccaactta 
     7321 ttagaataaa tgttttctat tttttacttg tatgtgtgag tgcctgcatg tatgtatgtg 
     7381 aagcatatgt attccaggat gtcagaattg tgatggattc cctggaactg gagttacaga 
     7441 ccattgtgaa ccactgtgta tgggtgctgg gaactgaact ttaggtcctc tgcaaaaaca 
     7501 gtaaggatct taactgataa actatctttc tagccctaag gctttctact tttaaagttc 
     7561 gtttctcttt ctctctctct ctctctctct ctctctctct ctccctctcc ctccctcctc 
     7621 ctcctcctcc tctttccccc ctctcgcctc ctctttctct ctcccctccc tctctctctc 
     7681 tcacacacac acacagacag agagaggaga gagaaaggaa gccttactaa ttaattacct 
     7741 agttttagac ataactagtg ggaatccata gtaagtaaat caacattctt gaagagaggt 
     7801 tagaagaatt ggctctcgtt gtccatcgag gctgtataag attattcttc tgttttctca 
     7861 gcttaccata catattttct acgtagacaa taccaattca aatacactgt acgtaaagta 
     7921 tatgcgcaca cacacacaca cacacacaca cacacacaca caagttacat attgtcatat 
     7981 agtactaaaa tttatggact ttttcatatt aatacatcta gagctatcat gttttttatc 
     8041 ttttaaaaat tactttacgg ggctacagag atgtctcagc agttaggagc actgactgct 
     8101 cttccagagg tcctgagttc aattcccagc aaccacatgg tggctcacaa ccatctgtaa 
     8161 tgggatctga tccactctta tggtgtgtct gaagacagtg acagtgtact catatacatg 
     8221 aaataaatct ttaaaaaatt actttaccat cagggtatat atatatatat atatgtgtgt 
     8281 gtgtgtgtgt gtgtgtatga agtatttgct atttgagagg gtctcactgt ttagctcagt 
     8341 ctggcttgga acgttctgta tagcccagac tggcctcaac tcagcagtcc ccctgaaagg 
     8401 ctctcatatg tggagattac aggtatgtgc tactgtgccc agccctacat gttactttgt 
     8461 ttttgaaata gggtatcaaa tagcttagac tggcttctaa gtcagtgtgt agctaaggat 
     8521 ggtctgagct tctgaacctg ctacttcaac tcctggtgtg ccatcatggc cagtttgcac 
     8581 agttgtgaga gtagaactca ggacctccta tgtgttacta gacatgttct ctaccagctg 
     8641 agctacatcc caattcgcct acatttactt ttatttttta aaagatttat ttatgtatac 
     8701 agcactctgc ctgcatgtac atctgcatgc cagaagaggg caccagatct catcatacat 
     8761 agttgtgagc caccatgtgg ttgctgggga ttgaactcag ggcctctgga agagcagtca 
     8821 gtgctcttaa cctctgagcc atctctgtag ccccctacat ttattttaaa gattataggc 
     8881 tattctattc atagatgtgc cataactgat ttaaccagat cttcattatt ttgtacttag 
     8941 tagagtatat tagagggaat gaatcttaaa tacacatttt gacacttgct cagctttaga 
     9001 caagtaaaaa tcactggtca aggaaatggt gcttagtgga tgcttctgaa ctgtcaaatt 
     9061 gtcctccaac aagttacatc attttatact tccaacgtta tgtgagagtt catttttctt 
     9121 taggctcttc tagtatttta tatttgagaa ataaaattta accaaaattt gccaatctag 
     9181 taagcttgtt tttatttgta tctttaaatt ttatgataga tgttatagtg tactctcctc 
     9241 tcccctcccc tcccctctcc tcctcagcag gatcttgtta tgtagcccta agggatctaa 
     9301 tacttactct gtagtccagg ctgtccttca gctcatgaga cacaccatca tgcttggcat 
     9361 gtttcttgtt ctgaaaggta tctggtttta attttggttg agctggtgtg ttggtgtata 
     9421 tctgtaatct cagcacaggc aagtagagac aaggattatg attacagtcc agcctgggct 
     9481 atttagaggg attctcttta atctttcctc atgccccaaa atcttacctt tggtatatga 
     9541 aaaatagtct ccacttctgc aaaatattgc tttatgaagt aagaattaat tcccttcata 
     9601 gagacaagga atgtgtcctg taaaagttta atgtgtaaga agtatttgtt ataaaagata 
     9661 aatattcaga atcttctttt taattcctga ttttatttct ataggactga aagacttgct 
     9721 cgagatgtca tgaaggagat gggaggccat cacattgtgg ccctctgtgt gctcaagggg 
     9781 ggctataagt tctttgctga cctgctggat tacattaaag cactgaatag aaatagtgat 
     9841 agatccattc ctatgactgt agattttatc agactgaaga gctactgtgt aagtataatt 
     9901 aacttataat taaaaaaata gggccattct agttttatct atattttttt taaacttgtg 
     9961 caaactatgc tacataatca acttacttga ttatgtaata tatatagtaa tagttggaat 
    10021 tactattatt acatatttat ttttctgtgc tggggtagaa cccagggaac taagtactgt 
    10081 aataaccgag cttcattcat cctagtcatt tttaattttt tttttttttt ttgttttttc 
    10141 gagacaaggt ttctctgtgt agccctggct gtcctggaac tcactctgta gaccaggctg 
    10201 gcctcgaact cagaaatctg cctgcctctg cctcccagtg ctgagattaa tgacgtgcac 
    10261 caccactgcc tggcattttt ttaaagtttt aatttgagac cctttctcac taacttgctc 
    10321 acactatcct cgaactcatt acgtgggctg gtctagaaat ttcagtcaac ttgcctcagc 
    10381 ttctcaagta gctgggatta caggcaggtg ctaccacttc tggccatgat cattgttgtt 
    10441 gttgctatta ttattattat tatttcactg atacttcata tcacaacaca gaatagtaac 
    10501 tgtagttcat gaccaacaaa aaagttgtac agtttctctg ttgtcttatt ttgatctgga 
    10561 ttttgatttg tttcatggca ctgggcaggc taatctcaaa ctgcatggct caaatagtcc 
    10621 ttctgtctta atctcccatg ttctggaagt ataggtatca ctacacctag ctgtattttc 
    10681 atattttaaa aaaaacgcct aagaattgct attgaatgag gcggagagat ggctcagcag 
    10741 tcacaagtac ttgtagagga ctggggtttg gttactagca cctcaaagat ggcttacaat 
    10801 tatctgtagc tccaattcca gaggatctgt gtggtaaagc tgcatgtata tgggcatgca 
    10861 ggtaaaactg ctaaacatat aaaaatagct cagtcttaag agaattggtc gactgtcgac 
    10921 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg 
    10981 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt 
    11041 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa 
    11101 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 
    11161 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 
    11221 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 
    11281 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 
    11341 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 
    11401 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 
    11461 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg 
    11521 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 
    11581 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 
    11641 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 
    11701 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 
    11761 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 
    11821 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 
    11881 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 
    11941 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 
    12001 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 
    12061 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 
    12121 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 
    12181 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 
    12241 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 
    12301 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca ggtcgagtag ggataacagg 
    12361 gtaatggatc ctcgttgggc acctgcactc atgtacgcag gcccacatat agatgtacac 
    12421 acgcatatca ttcaaaatag aaattattta tacaagtagt gcgttggaga gctggctcag 
    12481 gagttgttaa tgcttgctac ccttgcaaag gacccaagtt caatttccag cacctgtgtt 
    12541 gggcatctca caaccacatg taaatctagt ttcaggggat ttcacccctc ttctgaactc 
    12601 ttcaggcact tatacccaca aatggacatc tccacataga gacacatact tttaaaaaag 
    12661 taatctaggg tctagagaaa tggttataga ccacggactg ggatcagttc ccagaagcca 
    12721 catcctgtgg ctcacaattg cctgtgaatc aagttctaga tctggtggcc tcgtctagcc 
    12781 tgcagtcatc aacaaacaca tgcacacatc catacacaca taatttaaaa gaaagcgaaa 
    12841 gtaacctata atgctaaaca aagcacattc tcagtgtaga ctgcagtggt gatgaacctt 
    12901 gaaaatatat taatagaagc cagacacgta agtcacaaat ggtataactc aatttatatg 
    12961 aaattcccag agagtccaaa gttaaaaaaa acaaaaaggc agattattgg ttgtcagtag 
    13021 ctacagagat gggacattga agatattaat aggtataggg ttgaggattc atggcctttc 
    13081 cctcttccat gttagcatgt ctattgttgt taaccttatt caactcatgt tagggcaatc 
    13141 atattgatga gacttaatga gtatagcttg acattcctag aagacagagt ctcacatcag 
    13201 actctctgat cctctgactc ttagaatctt tcaacaccct cttctgaaat gatctctgag 
    13261 cctttggttg tgactgaatt atgagaaaga attttatgtt gaatgaattt tacttcagca 
    13321 tttttacaaa actgaagctg gggatggcgt ttcatgcgtg cagtccctac catttgccta 
    13381 taagcctgga ggaggcagaa gtagaattag gcttttcagg gacatcttca gctgcatcat 
    13441 gagcttgagg acaacttgag ctacataaga ttcccatccc cacccccaca cacacacaca 
    13501 catctagtat gtacctagta tgcagtcttt tcatatgttt aaaatgtaga aaggtatatg 
    13561 tgaagtctcc cttttcccca caacttagtc cctccaaagt attgcatcag tttccttgta 
    13621 catgatttta tgcatatata agatacacta tttctctctc tctctctctc cttccctcct 
    13681 tccctccaac ctctgggtat gagttgaggg ttatgtatgt ttcaagttgt aataagaatt 
    13741 gccactcaaa catttcatcc cattttaagt taagtttgtc tgattttcca ctttagaagg 
    13801 gcagccatga tggctcatgc ctataatccc atcctagcac ttgtaagcct aatacataag 
    13861 actggaggat tgtcatcagc ttgaggctag cctggtctac ctaatgagtt ccagccagcc 
    13921 aagagtagaa tataagaccc tgtctccaaa aaatgtacca aaggggctgg aggggtaact 
    13981 cagtggttaa gaacagtagc tgctcttcca gaggacctgg gttcaattcc cagcacccac 
    14041 atggcagctt gcaactatct gtaactgtcc agctttggga tatccgacac cattatacca 
    14101 attacataaa atgaaaaaat tgtaaaatgt gctaaaatga gtggggaggg ggagcatggt 
    14161 gtggttctgt gggcttggga ttttgccatg aatccatgac tatatctcag caatatgtgg 
    14221 attatttctg gaaacctgtg gggcatgggt gtatgggaag aggggagaga gagagagccc 
    14281 gcatccagcc agagttcctg tgctctgggc aggcggacgt gggaggactg ctggacgatt 
    14341 tccacttggc cctgggtggg catctggctg tgtgaagcca ctgaccccac tcagcggggg 
    14401 gtagataagg ggcatttccc ataccaggtt ctcagactcc agcattccac gctggataca 
    14461 gcagaagagc tgagaacaga ataggggccc cagggagaca ctctcgtccc agagataaag 
    14521 aaggcagagg gagagagatt cctacgcagg cgagaggcct tagtctgggc cttgatgaac 
    14581 agagacggtc tatggtttta gagctttatt gtagaaaggc agggggaaag agagaaggta 
    14641 gaaagagaga gaccagccat ggccaagagg agagaagggg ggaaaggaga gagagaagaa 
    14701 aggctagaga gtaagaaagg ttaaagagac agaagtaaaa gcttagagta agagagagag 
    14761 agagagcaag agggagagta agaaaagtaa aagcttaaga gagcgaggag gggccagccc 
    14821 ctctaatagt gggcttgtta tcttgttgtt gctaggtaac tgtgaagcgt ctagcctgaa 
    14881 ggccagaagc ttgggacatt gtctatgtga ctgaaagcca tgagcctcta ctgtgggggc 
    14941 tgtggaggtg gtaacttgga caggagccag gggttccagg agacatgagg gaatgccttc 
    15001 tgtcccatgt aggtaggaat taccaccacc ggctttcacc gtagttcact ggagactgtt 
    15061 tgtacatagc ccattgcccc acagaaacca ggcactcact gtttcatttg cataagaatt 
    15121 agaaggcttt attttggctg ggattggtga cttgccccag aaattccagc ttttgggaag 
    15181 ccgaatcctg gagaaccagg agttcaaggt catcctcagg tacctcattt gttcaagacc 
    15241 agcctgtgct accatgagac cttgtcccta ctcccctcaa ccccaaaaca cgctgttttc 
    15301 attttatagg ttgtactaac catggaggat ggtcatttgc ctttctcttg atttcagaag 
    15361 cgtagcgcgg gctttgcacc atgatctagt gtttccagat aggcttgcct ctccacagcc 
    15421 aggcttatcc tgcttcagtc ccatgtttgg caagggcaac atatgactga agaacttgca 
    15481 tagccttatg gtgtatcatg actcttagcc ccagtttata actgtctaac agctggtggc 
    15541 ttttgtgaaa aaggaaagca tcatttgaac ttttaacttt tcagctgttt gttttgactt 
    15601 tgcataaata ctgagactag ctaggaaatg agcttttgtt tcatttgcta aaaatgattt 
    15661 ttttttcatg aggcaatttc aagcaatttg aatttagcaa tctctacttg tctgaaaaga 
    15721 agttattaat taaaaaaaaa caaacaaaca ggaacctatt atgctggcta gtcacatgaa 
    15781 agaatccaaa tgaacacagt caaggctgaa tcaacacatg aaattatttt ctaattcccc 
    15841 aaattacatt tatgctaatt agtatgtagt aaaacatttt aattgtttcc aatgttaaac 
    15901 attacagaaa taattttgat gttttcctaa taacccagcc tttgatcatg tttgcattta 
    15961 tttgattgtt gtcagtttat taaataatta agtaagagca acaagataaa atgacttcat 
    16021 gagagtcctt ttctgtaact tagaacgtca gtagtcatag gaactgcggt cgttccctta 
    16081 cctttcagaa gtaggctaaa gagttgaacg caaaggtggt ttttcgaatc ttcctgatcc 
    16141 ttaagccccc actaggttta tattctatat ttgatatcgg attcgaaccc ctcgagctta 
    16201 ggatccggaa cccttaatat aactaagtta ttaggtccct cgaagaggtt cactagtact 
    16261 ggccattgcg gccgcttcga ggctgcctcg cgcgtttcgg tgatgacggt gaaaacctct 
    16321 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac 
    16381 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt 
    16441 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact 
    16501 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 
    16561 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 
    16621 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 
    16681 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 
    16741 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 
    16801 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 
    16861 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 
    16921 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 
    16981 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 
    17041 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 
    17101 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 
    17161 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 
    17221 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 
    17281 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 
    17341 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 
    17401 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 
    17461 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 
    17521 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 
    17581 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 
    17641 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 
    17701 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 
    17761 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 
    17821 tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 
    17881 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 
    17941 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 
    18001 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 
    18061 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 
    18121 gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 
    18181 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 
    18241 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 
    18301 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 
    18361 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 
    18421 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 
    18481 tccccgaaaa gtgccacctg acgg 
//
