LOCUS       pAAV\TBG\FFluc\m        6044 bp    DNA     circular     24-FEB-2012
SOURCE      
  ORGANISM  
COMMENT     Component Fragments
              #1: FRAGMENT of <a href="vnti:dnarna/pENN%20AAV%20CMV%20PI?sel=722-207">pENN AAV CMV PI</a> 
                parent position: from 722 to 207
                original length: 3753
                molecule position: from 920 to 202
                Left Terminus
                    HindIII site #1
                      Completely filled in
                Right Terminus
                    SphI site #1
                      3'-5' exonuclease treated (4 bp)
              #2: FRAGMENT of <a href="vnti:dnarna/pAAV%20TBG%20null%20seq?sel=192-908">pAAV TBG null seq</a> 
                parent position: from 192 to 908
                original length: 717
                molecule position: from 203 to 919
                Left Terminus
                    EcoRI site #1
                      Completely filled in
                Right Terminus
                    PstI site #1
                      3'-5' exonuclease treated (4 bp)
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|618693242|
COMMENT     VNTDBDATE|618693242|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV TBG FFluc miR122sponge|
COMMENT     VNTAUTHORNAME|JunXie|
COMMENT     VNTAUTHORTEL|508-856-3316|
COMMENT     VNTAUTHORFAX|508-856-1552|
COMMENT     VNTAUTHOREML|jun.xie@umassmed.edu|
COMMENT     VNTAUTHORAD1|University of Massachusetts Medical School|
COMMENT     VNTAUTHORAD2|381 Plantation St., Biotech 5, Ste. 250|
COMMENT     VNTAUTHORAD3|Worcester, MA 01605|
COMMENT     VNTOAUTHORNAME|Demo User|
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FEATURES             Location/Qualifiers
     rep_origin      complement(3401..3856)
                     /vntifkey="33"
                     /label=f1\ori
     misc_marker     3987..4844
                     /vntifkey="22"
                     /label=AP(R)
     rep_origin      5018..5606
                     /vntifkey="33"
                     /label=Origin\of\replication
     repeat_region   complement(3095..3224)
                     /vntifkey="34"
                     /label=3'\ITR
     repeat_region   2..131
                     /vntifkey="34"
                     /label=5'\ITR
     polyA_signal    2880..3006
                     /vntifkey="25"
                     /label=Rabbit\globin\poly\A
     enhancer        328..427
                     /vntifkey="9"
                     /label=Alpha\mic/bik
     enhancer        222..321
                     /vntifkey="9"
                     /label=Alpha\mic/bik
     TATA_signal     886..889
                     /vntifkey="41"
                     /label=TATA
     promoter        443..902
                     /vntifkey="29"
                     /label=TBG
     CDS             1003..2655
                     /gene="luc+"
                     /codon_start=1
                     /product="luciferase"
                     /protein_id="AAA89084.1"
                     /db_xref="GI:1200463"
                     /vntifkey="4"
                     /label=luc+
     primer_bind     complement(1004..1026)
                     /vntifkey="28"
                     /label=Primer_Bind_1
     misc_binding    2658..2814
                     /vntifkey="20"
                     /label=miR122\Sponge
BASE COUNT     1556 a      1498 c      1426 g      1564 t 
ORIGIN
        1 gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt 
       61 tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac 
      121 taggggttcc ttgtagttaa tgattaaccc gccatgctac ttatctacca gggtaatggg 
      181 gatcctctag aactatagct agaattcgcc cttaagctag caggttaatt tttaaaaagc 
      241 agtcaaaagt ccaagtggcc cttggcagca tttactctct ctgtttgctc tggttaataa 
      301 tctcaggagc acaaacattc cagatccagg ttaattttta aaaagcagtc aaaagtccaa 
      361 gtggcccttg gcagcattta ctctctctgt ttgctctggt taataatctc aggagcacaa 
      421 acattccaga tccggcgcgc cagggctgga agctaccttt gacatcattt cctctgcgaa 
      481 tgcatgtata atttctacag aacctattag aaaggatcac ccagcctctg cttttgtaca 
      541 actttccctt aaaaaactgc caattccact gctgtttggc ccaatagtga gaactttttc 
      601 ctgctgcctc ttggtgcttt tgcctatggc ccctattctg cctgctgaag acactcttgc 
      661 cagcatggac ttaaacccct ccagctctga caatcctctt tctcttttgt tttacatgaa 
      721 gggtctggca gccaaagcaa tcactcaaag ttcaaacctt atcatttttt gctttgttcc 
      781 tcttggcctt ggttttgtac atcagctttg aaaataccat cccagggtta atgctggggt 
      841 taatttataa ctaagagtgc tctagttttg caatacagga catgctataa aaatggaaag 
      901 atgttgcttt ctgagagaca gctttattgc ggtagtttat cacagttaaa ttgctaacgc 
      961 agtcagtgct tctgacacaa cagtctcgaa cttaagctgc acatggaaga cgccaaaaac 
     1021 ataaagaaag gcccggcgcc attctatccg ctggaagatg gaaccgctgg agagcaactg 
     1081 cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac agatgcacat 
     1141 atcgaggtgg acatcactta cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct 
     1201 atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga aaactctctt 
     1261 caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac 
     1321 gacatttata atgaacgtga attgctcaac agtatgggca tttcgcagcc taccgtggtg 
     1381 ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc 
     1441 caaaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc gatgtacacg 
     1501 ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtgcc agagtccttc 
     1561 gatagggaca agacaattgc actgatcatg aactcctctg gatctactgg tctgcctaaa 
     1621 ggtgtcgctc tgcctcatag aactgcctgc gtgagattct cgcatgccag agatcctatt 
     1681 tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt ccatcacggt 
     1741 tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt cttaatgtat 
     1801 agatttgaag aagagctgtt tctgaggagc cttcaggatt acaagattca aagtgcgctg 
     1861 ctggtgccaa ccctattctc cttcttcgcc aaaagcactc tgattgacaa atacgattta 
     1921 tctaatttac acgaaattgc ttctggtggc gctcccctct ctaaggaagt cggggaagcg 
     1981 gttgccaaga ggttccatct gccaggtatc aggcaaggat atgggctcac tgagactaca 
     2041 tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg taaagttgtt 
     2101 ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg cgttaatcaa 
     2161 agaggcgaac tgtgtgtgag aggtcctatg attatgtccg gttatgtaaa caatccggaa 
     2221 gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat agcttactgg 
     2281 gacgaagacg aacacttctt catcgttgac cgcctgaagt ctctgattaa gtacaaaggc 
     2341 tatcaggtgg ctcccgctga attggaatcc atcttgctcc aacaccccaa catcttcgac 
     2401 gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt 
     2461 ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta 
     2521 acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt 
     2581 accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa gaagggcgga 
     2641 aagatcgccg tgtaattcta gacaaacacc atacaacact ccacaaacac catacaacac 
     2701 tccacaaaca ccatacaaca ctccacaaac accatacaac actccacaaa caccatacaa 
     2761 cactccacaa acaccataca acactccaca aacaccatac aacactccag ggcccgcgtg 
     2821 gtacctctag agtcgacccg ggcggcctcg aggacggggt gaactacgcc tgaggatccg 
     2881 atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt 
     2941 ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct 
     3001 cactcggaag caattcgttg atctgaattt cgaccaccca taatacccat taccctggta 
     3061 gataagtagc atggcgggtt aatcattaac tacaaggaac ccctagtgat ggagttggcc 
     3121 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc 
     3181 ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagccttaa ttaacctaat 
     3241 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat 
     3301 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat 
     3361 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca 
     3421 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 
     3481 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 
     3541 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac 
     3601 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt 
     3661 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga 
     3721 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg 
     3781 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata 
     3841 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 
     3901 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 
     3961 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc 
     4021 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa 
     4081 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg 
     4141 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag 
     4201 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc 
     4261 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta 
     4321 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg 
     4381 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca 
     4441 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac 
     4501 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat 
     4561 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg 
     4621 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata 
     4681 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta 
     4741 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa 
     4801 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag 
     4861 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 
     4921 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 
     4981 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 
     5041 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 
     5101 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 
     5161 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 
     5221 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 
     5281 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 
     5341 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 
     5401 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 
     5461 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 
     5521 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 
     5581 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 
     5641 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 
     5701 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca 
     5761 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc 
     5821 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg 
     5881 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta 
     5941 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca 
     6001 gctatgacca tgattacgcc agatttaatt aaggccttaa ttag 
//
