LOCUS       pSimple\II-NLS-C        9281 bp    DNA     circular      4-JUN-2013
DEFINITION  general stem cell expression vector.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|652291531|
COMMENT     VNTDBDATE|663961313|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pSimple II-NLS-CSN1-HA-NLS-710-U6-tracr-U6-BsmBI|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Animal/Other Eukaryotic
COMMENT     VNTUDF|Created by|4|Takashi Kameda|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
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COMMENT       (CObList 
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COMMENT        #2=(CFSignal (CObList) "BGH polyA" 25 0 0 5533 5739 0 (CStringList) 
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COMMENT        #3=(CFSignal (CObList) "mini OriP" 33 0 0 5964 7086 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #4=(CFSignal (CObList) "pUC" 33 0 0 7467 8141 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #5=(CFSignal (CObList) "AP(R)" 4 0 1 8288 9145 0 (CStringList) 
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COMMENT        #6=(CFSignal (CObList) "nmCAS9" 4 0 0 2228 5470 0 (CStringList) 
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COMMENT        #7=(CFSignal (CObList) "NLS" 21 0 0 2204 2227 0 (CStringList) 
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COMMENT        #8=(CFSignal (CObList) "HA tag" 21 0 0 5471 5497 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #9=(CFSignal (CObList) "NLS-710" 21 0 0 5498 5530 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #10=(CFSignal (CObList) "TRACR-CSN1" 21 0 1 413 510 1 (CStringList) 
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COMMENT        #12=(CFSignal (CObList) "U6 promoter" 29 0 1 36 299 1 (CStringList) 
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COMMENT                          "Created: 01/29/13 04:05PM" 1) 
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FEATURES             Location/Qualifiers
     promoter        1011..2180
                     /vntifkey="29"
                     /label=EF1a\P
                     /note="EF1 alpha promoter"
     polyA_signal    5533..5739
                     /vntifkey="25"
                     /label=BGH\polyA
                     /note="BGH poly A signal (Bovine growth hormone)"
     rep_origin      5964..7086
                     /vntifkey="33"
                     /label=mini\OriP
                     /note="mini OriP (short version of OriP, allows plasmid replication in stem cells)"
     rep_origin      7467..8141
                     /vntifkey="33"
                     /label=pUC
                     /note="pUC bacterial origin of replication"
     CDS             complement(8288..9145)
                     /vntifkey="4"
                     /label=AP(R)
                     /note="ampicillin resistance"
     CDS             2228..5470
                     /vntifkey="4"
                     /label=nmCAS9
     misc_feature    2204..2227
                     /vntifkey="21"
                     /label=NLS
     misc_feature    5471..5497
                     /vntifkey="21"
                     /label=HA\tag
     misc_feature    5498..5530
                     /vntifkey="21"
                     /label=NLS-710
     misc_feature    complement(413..510)
                     /vntifkey="21"
                     /label=TRACR-CSN1
     terminator      complement(9..15)
                     /vntifkey="43"
                     /label=PolIII\terminator
     promoter        complement(36..299)
                     /vntifkey="29"
                     /label=U6\promoter
     terminator      complement(406..412)
                     /vntifkey="43"
                     /label=PolIII\terminator
     promoter        complement(511..774)
                     /vntifkey="29"
                     /label=U6\promoter
BASE COUNT     2464 a      2295 c      2299 g      2223 t 
ORIGIN
        1 gtttaaacaa aaaaatgaga cgtggaatcg tctccggtgt ttcgtccttt ccacaagata 
       61 tataaagcca agaaatcgaa atactttcaa gttacggtaa gcatatgata gtccatttta 
      121 aaacataatt ttaaaactgc aaactaccca agaaattatt actttctacg tcacgtattt 
      181 tgtactaata tctttgtgtt tacagtcaaa ttaattctaa ttatctctct aacagccttg 
      241 tatcgtatat gcaaatatga aggaatcatg ggaaataggc cctcttcctg cccgacctta 
      301 gatccgaatc catatcgata tgctagttat tgctcagcgg tggcagcagc caactcagcc 
      361 tatagtgagt cgtattaatt tcgcgggatg tttaaacgcg gccgcaaaaa aaccgaaata 
      421 aacgatgccc cttaaagcag aagctttaag gggcagagcg ttgcggcaca tcttttcaga 
      481 cggccttatt gtagcaacgg ttctcatttc ggtgtttcgt cctttccaca agatatataa 
      541 agccaagaaa tcgaaatact ttcaagttac ggtaagcata tgatagtcca ttttaaaaca 
      601 taattttaaa actgcaaact acccaagaaa ttattacttt ctacgtcacg tattttgtac 
      661 taatatcttt gtgtttacag tcaaattaat tctaattatc tctctaacag ccttgtatcg 
      721 tatatgcaaa tatgaaggaa tcatgggaaa taggccctct tcctgcccga ccttgacgtc 
      781 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
      841 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      901 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      961 ttagggttag gcgttttgcg ctgcttcggg ggtgaggctc cggtgcccgt cgtgaggctc 
     1021 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg 
     1081 ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt 
     1141 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt 
     1201 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg 
     1261 tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc 
     1321 cacctggctc cagtacgtga ttcttgatcc cgagctggag ccaggggcgg gccttgcgct 
     1381 ttaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc 
     1441 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt 
     1501 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg 
     1561 ggccaggatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt 
     1621 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga 
     1681 cgggggtcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc 
     1741 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag 
     1801 cggaaagatg gccgcttccc ggccctgctc cagggggctc aaaatggagg acgcggcgct 
     1861 cgggagagcg ggcgggtgag tcacccacac aaaggaaagg ggcctttccg tcctcagccg 
     1921 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctgga 
     1981 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc 
     2041 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg 
     2101 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa 
     2161 gtttttttct tccatttcag gtgtcgtgaa caccgccacc atggtgccta agaagaagag 
     2221 aaaggtggct gccttcaaac ctaattcaat caactacatc ctcggcctcg atatcggcat 
     2281 cgcatccgtc ggctgggcga tggtagaaat tgacgaagaa gaaaacccca tccgcctgat 
     2341 tgatttgggc gtgcgcgtat ttgagcgtgc cgaagtaccg aaaacaggcg actcccttgc 
     2401 catggcaagg cgtttggcgc gcagtgttcg ccgcctgacc cgccgtcgcg cccaccgcct 
     2461 gcttcggacc cgccgcctat tgaaacgcga aggcgtatta caagccgcca attttgacga 
     2521 aaacggcttg attaaatcct taccgaatac accatggcaa cttcgcgcag ccgcattaga 
     2581 ccgcaaactg acgcctttag agtggtcggc agtcttgttg catttaatca aacatcgcgg 
     2641 ctatttatcg caacggaaaa acgagggcga aactgccgat aaggagcttg gcgctttgct 
     2701 taaaggcgta gccggcaatg cccatgcctt acagacaggc gatttccgca caccggccga 
     2761 attggcttta aataaatttg agaaagaaag cggccatatc cgcaatcagc gcagcgatta 
     2821 ttcgcatacg ttcagccgca aagatttaca ggcggagctg attttgctgt ttgaaaaaca 
     2881 aaaagaattt ggcaatccgc atgtttcagg cggccttaaa gaaggtattg aaaccctact 
     2941 gatgacgcaa cgccctgccc tgtccggcga tgccgttcaa aaaatgttgg ggcattgcac 
     3001 cttcgaaccg gcagagccga aagccgctaa aaacacctac acagccgaac gtttcatctg 
     3061 gctgaccaag ctgaacaacc tgcgtatttt agagcaaggc agcgagcggc cattgaccga 
     3121 taccgaacgc gccacgctta tggacgagcc atacagaaaa tccaaactga cttacgcaca 
     3181 agcccgtaag ctgctgggtt tagaagatac cgcctttttc aaaggcttgc gctatggtaa 
     3241 agacaatgcc gaagcctcaa cattgatgga aatgaaggcc taccatgcca tcagccgtgc 
     3301 actggaaaaa gaaggattga aagacaaaaa atccccatta aacctttctc ccgaattaca 
     3361 agacgaaatc ggcacggcat tctccctgtt caaaaccgat gaagacatta caggccgtct 
     3421 gaaagaccgt atacagcccg aaatcttaga agcgctgttg aaacacatca gcttcgataa 
     3481 gttcgtccaa atttccttga aagcattgcg ccgaattgtg cctctaatgg aacaaggcaa 
     3541 acgttacgat gaagcctgcg ccgaaatcta cggagaccat tacggcaaga agaatacgga 
     3601 agaaaagatt tatctgccgc cgattcccgc cgacgaaatc cgcaaccccg tcgtcttgcg 
     3661 cgccttatct caagcacgta aggtcattaa cggcgtggta cgccgttacg gctccccagc 
     3721 tcgtatccat attgaaactg caagggaagt aggtaaatcg tttaaagacc gcaaagaaat 
     3781 tgagaaacgc caagaagaaa accgcaaaga ccgggaaaaa gccgccgcca aattccgaga 
     3841 gtatttcccc aattttgtcg gagaacccaa atccaaagat attctgaaac tgcgcctgta 
     3901 cgagcaacaa cacggcaaat gcctgtattc gggcaaagaa atcaacttag gccgtctgaa 
     3961 cgaaaaaggc tatgtcgaaa tcgaccatgc cctgccgttc tcgcgcacat gggacgacag 
     4021 tttcaacaat aaagtactgg tattgggcag cgaaaaccaa aacaaaggca atcaaacccc 
     4081 ttacgaatac ttcaacggca aagacaacag ccgcgaatgg caggaattta aagcgcgtgt 
     4141 cgaaaccagc cgtttcccgc gcagtaaaaa acaacggatt ctgctgcaaa aattcgatga 
     4201 agacggcttt aaagaacgca atctgaacga cacgcgctac gtcaaccgtt tcctgtgtca 
     4261 atttgttgcc gaccgtatgc ggctgacagg taaaggcaag aaacgtgtct ttgcatccaa 
     4321 cggacaaatt accaatctgt tgcgcggctt ttggggattg cgcaaagtgc gtgcggaaaa 
     4381 cgaccgccat cacgccttgg acgccgtcgt cgttgcctgc tcgaccgttg ccatgcagca 
     4441 gaaaattacc cgttttgtac gctataaaga gatgaacgcg tttgacggta aaaccataga 
     4501 caaagaaaca ggagaagtgc tgcatcaaaa aacacacttc ccacaacctt gggaattttt 
     4561 cgcacaagaa gtcatgattc gcgtcttcgg caaaccggac ggcaaacccg aattcgaaga 
     4621 agccgatacc ctagaaaaac tgcgcacgtt gcttgccgaa aaattatcat ctcgccccga 
     4681 agccgtacac gaatacgtta cgccactgtt tgtttcacgc gcgcccaatc ggaagatgag 
     4741 cgggcaaggg catatggaga ccgtcaaatc cgccaaacga ctggacgaag gcgtcagcgt 
     4801 gttgcgcgta ccgctgacac agttaaaact gaaagacttg gaaaaaatgg tcaatcggga 
     4861 gcgcgaacct aagctatacg aagcactgaa agcacggctg gaagcacata aagacgatcc 
     4921 tgccaaagcc tttgccgagc cgttttacaa atacgataaa gcaggcaacc gcacccaaca 
     4981 ggtaaaagcc gtacgcgtag agcaagtaca gaaaaccggc gtatgggtgc gcaaccataa 
     5041 cggtattgcc gacaacgcaa ccatggtgcg cgtagatgtg tttgagaaag gcgacaagta 
     5101 ttatctggta ccgatttaca gttggcaggt agcgaaaggg attttgccgg atagggctgt 
     5161 tgtacaagga aaagatgaag aagattggca acttattgat gatagtttca actttaaatt 
     5221 ctcattacac cctaatgatt tagtcgaggt tataacaaaa aaagctagaa tgtttggtta 
     5281 ctttgccagc tgccatcgag gcacaggtaa tatcaatata cgcattcatg atcttgatca 
     5341 taaaattggc aaaaatggaa tactggaagg tatcggcgtc aaaaccgccc tttcattcca 
     5401 aaaataccaa attgacgaac tgggcaaaga aatcagacca tgccgtctga aaaaacgccc 
     5461 gcctgtccgt tacccatacg atgttccaga ttacgctgca gctccagcag cgaagaaaaa 
     5521 gaagctggat taactcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt 
     5581 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc 
     5641 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt 
     5701 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat 
     5761 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgac cgatgccctt 
     5821 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc 
     5881 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgcctggcc ggggtccccc 
     5941 ggaaactcgg ccgtggtgac catgcaggaa aaggacaagc agcgaaaatt cacgccccct 
     6001 tgggaggtgg cggcatatgc aaaggatagc actcccactc tactactggg tatcatatgc 
     6061 tgactgtata tgcatgagga tagcatatgc tacccggata cagattagga tagcatatac 
     6121 tacccagata tagattagga tagcatatgc tacccagata tagattagga tagcctatgc 
     6181 tacccagata taaattagga tagcatatac tacccagata tagattagga tagcatatgc 
     6241 tacccagata tagattagga tagcctatgc tacccagata tagattagga tagcatatgc 
     6301 tacccagata tagattagga tagcatatgc tatccagata tttgggtagt atatgctacc 
     6361 cagatataaa ttaggatagc atatactacc ctaatctcta ttaggatagc atatgctacc 
     6421 cggatacaga ttaggatagc atatactacc cagatataga ttaggatagc atatgctacc 
     6481 cagatataga ttaggatagc ctatgctacc cagatataaa ttaggatagc atatactacc 
     6541 cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc ctatgctacc 
     6601 cagatataga ttaggatagc atatgctatc cagatatttg ggtagtatat gctacccatg 
     6661 gcaacattag cccaccgtgc tctcagcgac ctcgtgaata tgaggaccaa caaccctgtg 
     6721 cttggcgctc aggcgcaagt gtgtgtaatt tgtcctccag atcgcagcaa tcgcgcccct 
     6781 atcttggccc gcccacctac ttatgcaggt attccccggg gtgccattag tggttttgtg 
     6841 ggcaagtggt ttgaccgcag tggttagcgg ggttacaatc agccaagtta ttacaccctt 
     6901 attttacagt ccaaaaccgc agggcggcgt gtgggggctg acgcgatcgc tgttccttag 
     6961 gaccctttta ctaaccctaa ttcgatagca tatgcttccc gttgggtaac atatgctatt 
     7021 gaattagggt tagtctggat agtatatact actacccggg aagcatatgc tacccgttta 
     7081 gggttatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg 
     7141 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 
     7201 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 
     7261 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 
     7321 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 
     7381 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 
     7441 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 
     7501 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 
     7561 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 
     7621 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 
     7681 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc 
     7741 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 
     7801 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 
     7861 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 
     7921 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 
     7981 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 
     8041 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 
     8101 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 
     8161 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 
     8221 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 
     8281 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 
     8341 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 
     8401 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 
     8461 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 
     8521 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 
     8581 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 
     8641 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 
     8701 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 
     8761 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 
     8821 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 
     8881 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 
     8941 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 
     9001 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 
     9061 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 
     9121 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 
     9181 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 
     9241 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c 
//
