LOCUS       pAAVscCBPI\TuDmi        5355 bp    DNA     circular     22-FEB-2012
SOURCE      
  ORGANISM  
COMMENT       #1: FRAGMENT of <a href="vnti:dnarna/pENN%20AAVsc%20F?sel=165-152">pENN AAVsc F</a> 
                parent position: from 165 to 152 (+ fill of 4)
                original length: 3320
                molecule position: from 836 to 146
                Left Terminus
                    XbaI site #1
                Right Terminus
                    MluI site #1
                      Completely filled in
              #2: FRAGMENT of <a href="vnti:dnarna/pENN%20AAV%20CB6%20%28p1017%29?sel=203-891">pENN AAV CB6 (p1017)</a> 
                parent position: from 203 to 891
                original length: 689
                molecule position: from 147 to 835
                Left Terminus
                    SalI site #1
                      Completely filled in
                Right Terminus
                    XbaI site #2
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|618409635|
COMMENT     VNTDBDATE|618506271|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAVscCBPI TuDmiR122Gpluc7xlet7BT|
COMMENT     VNTAUTHORNAME|JunXie|
COMMENT     VNTAUTHORTEL|508-856-3316|
COMMENT     VNTAUTHORFAX|508-856-1552|
COMMENT     VNTAUTHOREML|jun.xie@umassmed.edu|
COMMENT     VNTAUTHORAD1|University of Massachusetts Medical School|
COMMENT     VNTAUTHORAD2|381 Plantation St., Biotech 5, Ste. 250|
COMMENT     VNTAUTHORAD3|Worcester, MA 01605|
COMMENT     VNTOAUTHORNAME|Gao|
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FEATURES             Location/Qualifiers
     CDS             3297..4157
                     /vntifkey="4"
                     /label=Amp-R
     repeat_region   complement(2405..2534)
                     /vntifkey="34"
                     /label=3'\ITR
     rep_origin      complement(2711..3166)
                     /vntifkey="33"
                     /label=f1\ori
     repeat_region   1..105
                     /vntifkey="34"
                     /label=ITR
     misc_feature    1..28
                     /vntifkey="21"
                     /label=repeat\FOR
     misc_feature    complement(68..95)
                     /vntifkey="21"
                     /label=repeat\REV
     misc_feature    4328..4916
                     /vntifkey="21"
                     /label=Origin
     misc_feature    183..468
                     /vntifkey="21"
                     /label=CMV\Ehancer
     promoter        472..736
                     /vntifkey="29"
                     /label=CB\Promoter
     misc_feature    636..642
                     /vntifkey="21"
                     /label=CAAT\box
     misc_feature    699..706
                     /vntifkey="21"
                     /label=TATA\box
     intron          771..1407
                     /vntifkey="15"
                     /label=intron
     misc_feature    828..1288
                     /vntifkey="21"
                     /label=TuD\122
     polyA_signal    2169..2364
                     /vntifkey="25"
                     /label=BGH\3'UTR+PolyA+GRE\(partial)
     CDS             1439..1996
                     /vntifkey="4"
                     /label=Guassia
     misc_feature    2011..2162
                     /vntifkey="21"
                     /label=7xlet7BT
BASE COUNT     1305 a      1390 c      1379 g      1281 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtgtagcc atgctctagg 
      121 aagatcaatt cggtacaatt cacgcgtcga cattgattat tgactctggt cgttacataa 
      181 cttacggtaa atggcccgcc tggctgaccg cccaacgacc ccgcccattg acgtcaataa 
      241 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt 
      301 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc 
      361 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat 
      421 gggactttcc tacttggcag tacatctact cgaggccacg ttctgcttca ctctccccat 
      481 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc 
      541 gatgggggcg gggggggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg 
      601 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa 
      661 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc 
      721 gggcgggagc gggatcagcc accgcggtgg cggccctaga gtcgatcgag gaactgaaaa 
      781 accagaaagt taactggtaa gtttagtctt tttgtctttt atttcagccc cagtggaaag 
      841 acgcgcaggc aaaacgcacc acgtgacgga gcgtgaccgc gcgccgagcg cgcgccaagg 
      901 tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata cgatacaagg 
      961 ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta gtacaaaata 
     1021 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa 
     1081 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttgggt ttatatatct 
     1141 tgtggaaagg acgcgggatc cgacggcgct aggatcatca accaaacacc attgatcttc 
     1201 acactccaca agtattctgg tcacagaata caaccaaaca ccattgatct tcacactcca 
     1261 caagatgatc ctagcgccgt cttttttaag ctgtcccgga tccggtggtg gtgcaaatca 
     1321 aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa gtgttacttc 
     1381 tgctctaaaa gctgcggaat tgtacccgcg gccgatccac cggtcgccac catctagcat 
     1441 gggagtcaaa gttctgtttg ccctgatctg catcgctgtg gccgaggcca agcccaccga 
     1501 gaacaacgaa gacttcaaca tcgtggccgt ggccagcaac ttcgcgacca cggatctcga 
     1561 tgctgaccgc gggaagttgc ccggcaagaa gctgccgctg gaggtgctca aagagatgga 
     1621 agccaatgcc cggaaagctg gctgcaccag gggctgtctg atctgcctgt cccacatcaa 
     1681 gtgcacgccc aagatgaaga agttcatccc aggacgctgc cacacctacg aaggcgacaa 
     1741 agagtccgca cagggcggca taggcgaggc gatcgtcgac attcctgaga ttcctgggtt 
     1801 caaggacttg gagcccatgg agcagttcat cgcacaggtc gatctgtgtg tggactgcac 
     1861 aactggctgc ctcaaagggc ttgccaacgt gcagtgttct gacctgctca agaagtggct 
     1921 gccgcaacgc tgtgcgacct ttgccagcaa gatccagggc caggtggaca agatcaaggg 
     1981 ggccggtggt gactagctcg acgctgatcc tagaaactat acaaaaccta cctcaaacca 
     2041 cacaaaacct acctcaaacc atacaaaacc tacctcaaac tatgcaaaac ctacctctaa 
     2101 ctatacaaaa cctacctcaa actgtacaaa acctacctca aaccatacaa aacctacctc 
     2161 agatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg 
     2221 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt 
     2281 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc 
     2341 aagggggagg attgggaaga caattaggta gataagtagc atggcgggtt aatcattaac 
     2401 tacaaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact 
     2461 gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc 
     2521 gagcgagcgc gcagccttaa ttaacctaat tcactggccg tcgttttaca acgtcgtgac 
     2581 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc 
     2641 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat 
     2701 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 
     2761 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 
     2821 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 
     2881 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 
     2941 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 
     3001 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 
     3061 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 
     3121 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc 
     3181 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 
     3241 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga 
     3301 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 
     3361 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 
     3421 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 
     3481 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta 
     3541 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 
     3601 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 
     3661 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 
     3721 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 
     3781 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 
     3841 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 
     3901 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 
     3961 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 
     4021 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 
     4081 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 
     4141 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 
     4201 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 
     4261 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 
     4321 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 
     4381 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 
     4441 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc 
     4501 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 
     4561 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 
     4621 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 
     4681 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 
     4741 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 
     4801 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 
     4861 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 
     4921 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 
     4981 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 
     5041 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc 
     5101 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg 
     5161 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca 
     5221 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg 
     5281 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc agatttaatt 
     5341 aaggccttaa ttagg 
//
