LOCUS Ple147\(pEMS1397) 18813 bp DNA circular 31-JAN-2011 DEFINITION pEMS1306_Seq_Verified backbone. SOURCE ORGANISM COMMENT NOTE: Insert was not sequence confirmed, ends may be different and may contain some internal SNPs (sequence is based on initial cloning design, and not necessarirly final cloning design) COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|580574154| COMMENT VNTDBDATE|580574154| COMMENT LSOWNER| COMMENT VNTNAME|Ple147 (pEMS1397)| COMMENT VNTAUTHORNAME|Russell Bonaguro| COMMENT VNTOAUTHORNAME|George Yang| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "Ple147 (pEMS1397)" 0 18813 COMMENT (CDBMol 0 0 1 1 1 0 0 1 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) COMMENT (CObList) -1 "") COMMENT (CDocSetData 1 1 0 1 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 200 COMMENT 0 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) (CStringList) COMMENT (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 COMMENT "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 0 4294967295 1 18813 0 0 0 0 0 0 0 0) (CStringList) COMMENT (CStringList) (CObList) (COAPar 25 250 50 0 20 20 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) COMMENT (CObList COMMENT #0=(CFSignal (CObList) "5' arm of Hprt homology" 86 0 0 12676 16487 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "12676..16487") COMMENT #1=(CFSignal (CObList) "AmpR" 22 0 0 17807 18807 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "17807..18807") COMMENT #2=(CFSignal (CObList) "AmpR" 22 0 0 11225 12650 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "11225..12650") COMMENT #3=(CFSignal (CObList) "3' arm of Hprt homology" 86 0 0 5408 11229 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "5408..11229") COMMENT #4=(CFSignal (CObList) "HPRT complementary sequence" 86 0 0 3630 5407 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "3630..5407") COMMENT #5=(CFSignal (CObList) "I-Sce I" 21 0 0 12657 12674 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "12657..12674") COMMENT #6=(CFSignal (CObList) "FRT (F5mutant)" 21 0 0 2512 2545 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "2512..2545") COMMENT #7=(CFSignal (CObList) "Kozak consensus" 21 0 0 2546 2556 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "2546..2556") COMMENT #8=(CFSignal (CObList) "EGFP (mutated stop TAA -> TTA)" 21 0 0 2553 3272 COMMENT 0 (CStringList) (CStringList) 1 1 1 1 "2553..3272") COMMENT #9=(CFSignal (CObList) "Tetracysteine residue" 21 0 0 3273 3308 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "3273..3308") COMMENT #10=(CFSignal (CObList) "NLS" 21 0 0 3309 3381 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "3309..3381") COMMENT #11=(CFSignal (CObList) "FRT" 21 0 0 3387 3420 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "3387..3420") COMMENT #12=(CFSignal (CObList) "SV40 early polyA signal" 21 0 0 3574 3624 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "3574..3624") COMMENT #13=(CFSignal (CObList) "Prom region" 21 0 0 1462 2489 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1462..2489") COMMENT #14=(CFSignal (CObList) "oEMS2607" 27 0 0 1678 1697 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1678..1697") COMMENT #15=(CFSignal (CObList) "oEMS2790" 27 0 0 1021 1042 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1021..1042") COMMENT #16=(CFSignal (CObList) "Ple147 (NTSR1-D)" 21 0 0 139 2489 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "139..2489")) (CObList) COMMENT (CObList COMMENT #17=(CPrimers (CStringList) (CStringList) COMMENT (CPrimer 0 "aaccatggttagggggtggg" 63 932 20 0 100 100 100 57.0166 COMMENT 60 -165.5 -423.8 -37.364 -18.6419) COMMENT (CPrimer 0 "ttggggtgagggtgtatccg" 63 1678 20 1 100 100 100 56.7477 COMMENT 60 -157.5 -401.4 -36.0426 -13.987) 171 932 1697 766 84.2319 COMMENT 61.4883 61.0866) COMMENT #18=(CPrimers (CStringList) (CStringList) COMMENT (CPrimer 0 "accatggttagggggtggga" 63 933 20 0 100 100 100 57.4373 COMMENT 60 -162 -413.3 -36.9946 -17.1477) COMMENT (CPrimer 0 "ttggggtgagggtgtatccg" 63 1678 20 1 100 100 100 56.7477 COMMENT 60 -157.5 -401.4 -36.0426 -13.987) 171 933 1697 765 84.2637 COMMENT 61.5686 61.1089) COMMENT #19=(CPrimers (CStringList) (CStringList) COMMENT (CPrimer 0 "caggaggacactgtgctggaag" 63 1020 22 0 100 100 100 COMMENT 56.1213 59.0909 -157.5 -402.2 -35.8041 -14.8788) COMMENT (CPrimer 0 "ttggggtgagggtgtatccg" 63 1678 20 1 100 100 100 56.7477 COMMENT 60 -157.5 -401.4 -36.0426 -13.987) 171 1020 1697 678 83.7613 COMMENT 60.6195 60.5693) COMMENT #20=(CPrimers (CStringList) (CStringList) COMMENT (CPrimer 0 "aggaggacactgtgctggaagg" 63 1021 22 0 100 100 100 COMMENT 56.9711 59.0909 -162.7 -415.9 -36.9194 -14.8086) COMMENT (CPrimer 0 "ttggggtgagggtgtatccg" 63 1678 20 1 100 100 100 56.7477 COMMENT 60 -157.5 -401.4 -36.0426 -13.987) 171 1021 1697 677 83.736 COMMENT 60.5613 60.7395)) (CObList) (CObList) (CObList) (CObList) COMMENT (CTextView 0 COMMENT #21=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #22=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) COMMENT "Ple147 (pEMS1397)" 2) 5 "" 0 4) COMMENT #23=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA 'Ple147 (pEMS1397)'" 1) COMMENT #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "pEMS1306_Seq_Verified backbone." 1) COMMENT #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Currently local object. Original author: George Yang" COMMENT 1) COMMENT #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Created: 01/31/11 02:35PM" 1) COMMENT #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Last Modified: 01/31/11 02:35PM" 1) COMMENT #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "length: 18813 bp" 1) COMMENT #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "storage type: Basic" 1) COMMENT #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "form: Circular" 1))) "General Description") COMMENT #32=(CFolderPar COMMENT (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Original Source Database: GenBank" 1) COMMENT #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Modification Date in the Original DB: 1-NOV-2007" 1))) COMMENT "Standard Fields") COMMENT #35=(CFolderPar COMMENT (CGroupPar (CParagraph 5 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "George Yang" 1))) "Original Author") COMMENT #37=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" COMMENT 0 0) COMMENT #38=(CFolderPar COMMENT (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) COMMENT "Annotations") COMMENT #39=(CFolderPar COMMENT (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #40=(CFolderPar COMMENT (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #41=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 139 (3 #16# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #42=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 139 End: 2489" 1) COMMENT #43=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #44=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 139..2489" 1))) "Ple147 (NTSR1-D)") COMMENT #45=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1462 (3 #13# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #46=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1462 End: 2489" 1) COMMENT #47=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #48=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1462..2489" 1))) "Prom region") COMMENT #49=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2512 (3 #6# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #50=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2512 End: 2545" 1) COMMENT #51=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #52=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 2512..2545" 1))) "FRT (F5mutant)") COMMENT #53=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2546 (3 #7# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #54=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2546 End: 2556" 1) COMMENT #55=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #56=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 2546..2556" 1))) "Kozak consensus") COMMENT #57=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2553 (3 #8# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #58=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2553 End: 3272" 1) COMMENT #59=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #60=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 2553..3272" 1))) COMMENT "EGFP (mutated stop TAA -> TTA)") COMMENT #61=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3273 (3 #9# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #62=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3273 End: 3308" 1) COMMENT #63=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #64=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 3273..3308" 1))) COMMENT "Tetracysteine residue") COMMENT #65=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3309 (3 #10# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #66=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3309 End: 3381" 1) COMMENT #67=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #68=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 3309..3381" 1))) "NLS") COMMENT #69=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3387 (3 #11# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #70=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3387 End: 3420" 1) COMMENT #71=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #72=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 3387..3420" 1))) "FRT") COMMENT #73=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3574 (3 #12# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #74=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3574 End: 3624" 1) COMMENT #75=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #76=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 3574..3624" 1))) COMMENT "SV40 early polyA signal") COMMENT #77=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 12657 (3 #5# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #78=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 12657 End: 12674" 1) COMMENT #79=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #80=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 12657..12674" 1))) "I-Sce I"))) COMMENT "Misc. Feature (10 total)") COMMENT #81=(CFolderPar COMMENT (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #82=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 11225 (3 #2# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #83=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 11225 End: 12650" 1) COMMENT #84=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #85=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 11225..12650" 1))) "AmpR") COMMENT #86=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 17807 (3 #1# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #87=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 17807 End: 18807" 1) COMMENT #88=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #89=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 17807..18807" 1))) "AmpR"))) COMMENT "Misc. Marker (2 total)") COMMENT #90=(CFolderPar COMMENT (CGroupPar (CParagraph 27 (7 27 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #91=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1021 (3 #15# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #92=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1021 End: 1042" 1) COMMENT #93=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) "57.0" COMMENT 1) COMMENT #94=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #95=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1021..1042" 1))) "oEMS2790") COMMENT #96=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1678 (3 #14# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #97=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1678 End: 1697" 1) COMMENT #98=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #99=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1678..1697" 1))) "oEMS2607"))) COMMENT "Primer (2 total)") COMMENT #100=(CFolderPar COMMENT (CGroupPar (CParagraph 86 (7 86 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #101=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3630 (3 #4# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #102=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3630 End: 5407" 1) COMMENT #103=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Consists of human HPRT promoter, human Exon1, Intron1, Exon2, and part of Intron2" COMMENT 1) COMMENT #104=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #105=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 3630..5407" 1))) COMMENT "HPRT complementary sequence") COMMENT #106=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 5408 (3 #3# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #107=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 5408 End: 11229" 1) COMMENT #108=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Consists of part of Intron 2, all of Exon 3, and part of Intron 3" COMMENT 1) COMMENT #109=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #110=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 5408..11229" 1))) COMMENT "3' arm of Hprt homology") COMMENT #111=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 12676 (3 #0# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #112=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 12676 End: 16487" 1) COMMENT #113=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "The 5 HPRT probe anneals to genomic sequence ~560bp upstream of the BamHI site at 9263" COMMENT 1) COMMENT #114=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #115=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 12676..16487" 1))) COMMENT "5' arm of Hprt homology"))) COMMENT "Misc. Recombination (3 total)"))) "Feature Map") COMMENT #116=(CFolderPar COMMENT (CGroupPar (CParagraph 14 (0 0) 1 2 0 0 178) COMMENT (CObjectList COMMENT #117=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT " Similarity between ambiguous nucleotides (if any)" COMMENT 1) COMMENT #118=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "is calculated as an AVERAGE similarity" 1))) COMMENT "Motifs (both strands, best fit only)") COMMENT #119=(CFolderPar COMMENT (CGroupPar (CParagraph 16 (0 0) 1 1 0 0 2104) COMMENT (CObjectList COMMENT #120=(CPCRFolderPar COMMENT (CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 0 COMMENT (20 COMMENT #121=(CPCRData (CStringList) (CStringList) COMMENT "aaccatggttagggggtggg" 0 COMMENT "ttggggtgagggtgtatccg" 0 932 1697 766 0 0) COMMENT 0) 1 2 1 0 182) COMMENT (CObjectList COMMENT #122=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Contains region of the molecule from 932 to 1697" COMMENT 1) COMMENT #123=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Tm: 84.2 C TaOpt: 61.1 C GC: 61.5" 1) COMMENT #124=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Sense Primer: " 1) COMMENT #125=(COlyLinePar COMMENT (CLinePar COMMENT (CParagraph 0 COMMENT (17 COMMENT #126=(COligo "" "AACCATGGTTAGGGGGTGGG" COMMENT "Sense primer for PCR amplification of a region of Ple147 (pEMS1397)" COMMENT 0 (CStringList) 0) 1) 1 2 2 0 181) COMMENT " AACCATGGTTAGGGGGTGGG" 1)) COMMENT #127=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Similarity: 100.0%" 1) COMMENT #128=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Length: 20 Tm: 57.0 C GC: 60.0" 1) COMMENT #129=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " dH: -165.5 kcal/mol dS: -423.8 cal/mol dG: -37.4 kcal/mol" COMMENT 1) COMMENT #130=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Antisense Primer: " 1) COMMENT #131=(COlyLinePar COMMENT (CLinePar COMMENT (CParagraph 0 COMMENT (17 COMMENT #132=(COligo "" "TTGGGGTGAGGGTGTATCCG" COMMENT "Antisense primer for PCR amplification of a region of Ple147 (pEMS1397)" COMMENT 0 (CStringList) 0) 1) 1 2 2 0 181) COMMENT " TTGGGGTGAGGGTGTATCCG" 1)) COMMENT #133=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Similarity: 100.0%" 1) COMMENT #134=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Length: 20 Tm: 56.7 C GC: 60.0" 1) COMMENT #135=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " dH: -157.5 kcal/mol dS: -401.4 cal/mol dG: -36.0 kcal/mol" COMMENT 1) COMMENT #136=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Tm Difference: 0.3" 1) COMMENT #137=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "GC Difference: 0.0" 1))) COMMENT "#1: Product of length 766 (rating: 171)")) COMMENT #138=(CPCRFolderPar COMMENT (CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 0 COMMENT (20 COMMENT #139=(CPCRData (CStringList) (CStringList) COMMENT "accatggttagggggtggga" 0 COMMENT "ttggggtgagggtgtatccg" 0 933 1697 765 0 0) COMMENT 0) 1 2 1 0 182) COMMENT (CObjectList COMMENT #140=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Contains region of the molecule from 933 to 1697" COMMENT 1) COMMENT #141=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Tm: 84.3 C TaOpt: 61.1 C GC: 61.6" 1) COMMENT #142=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Sense Primer: " 1) COMMENT #143=(COlyLinePar COMMENT (CLinePar COMMENT (CParagraph 0 COMMENT (17 COMMENT #144=(COligo "" "ACCATGGTTAGGGGGTGGGA" COMMENT "Sense primer for PCR amplification of a region of Ple147 (pEMS1397)" COMMENT 0 (CStringList) 0) 1) 1 2 2 0 181) COMMENT " ACCATGGTTAGGGGGTGGGA" 1)) COMMENT #145=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Similarity: 100.0%" 1) COMMENT #146=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Length: 20 Tm: 57.4 C GC: 60.0" 1) COMMENT #147=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " dH: -162.0 kcal/mol dS: -413.3 cal/mol dG: -37.0 kcal/mol" COMMENT 1) COMMENT #148=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Antisense Primer: " 1) COMMENT #149=(COlyLinePar COMMENT (CLinePar COMMENT (CParagraph 0 COMMENT (17 COMMENT #150=(COligo "" "TTGGGGTGAGGGTGTATCCG" COMMENT "Antisense primer for PCR amplification of a region of Ple147 (pEMS1397)" COMMENT 0 (CStringList) 0) 1) 1 2 2 0 181) COMMENT " TTGGGGTGAGGGTGTATCCG" 1)) COMMENT #151=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Similarity: 100.0%" 1) COMMENT #152=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Length: 20 Tm: 56.7 C GC: 60.0" 1) COMMENT #153=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " dH: -157.5 kcal/mol dS: -401.4 cal/mol dG: -36.0 kcal/mol" COMMENT 1) COMMENT #154=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Tm Difference: 0.7" 1) COMMENT #155=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "GC Difference: 0.0" 1))) COMMENT "#2: Product of length 765 (rating: 171)")) COMMENT #156=(CPCRFolderPar COMMENT (CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 0 COMMENT (20 COMMENT #157=(CPCRData (CStringList) (CStringList) COMMENT "caggaggacactgtgctggaag" 0 COMMENT "ttggggtgagggtgtatccg" 0 1020 1697 678 0 0) COMMENT 0) 1 2 1 0 182) COMMENT (CObjectList COMMENT #158=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Contains region of the molecule from 1020 to 1697" COMMENT 1) COMMENT #159=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Tm: 83.8 C TaOpt: 60.6 C GC: 60.6" 1) COMMENT #160=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Sense Primer: " 1) COMMENT #161=(COlyLinePar COMMENT (CLinePar COMMENT (CParagraph 0 COMMENT (17 COMMENT #162=(COligo "" "CAGGAGGACACTGTGCTGGAAG" COMMENT "Sense primer for PCR amplification of a region of Ple147 (pEMS1397)" COMMENT 0 (CStringList) 0) 1) 1 2 2 0 181) COMMENT " CAGGAGGACACTGTGCTGGAAG" 1)) COMMENT #163=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Similarity: 100.0%" 1) COMMENT #164=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Length: 22 Tm: 56.1 C GC: 59.1" 1) COMMENT #165=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " dH: -157.5 kcal/mol dS: -402.2 cal/mol dG: -35.8 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22 Tm: 57.0 C GC: 59.1" 1) COMMENT #183=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " dH: -162.7 kcal/mol dS: -415.9 cal/mol dG: -36.9 kcal/mol" COMMENT 1) COMMENT #184=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Antisense Primer: " 1) COMMENT #185=(COlyLinePar COMMENT (CLinePar COMMENT (CParagraph 0 COMMENT (17 COMMENT #186=(COligo "" "TTGGGGTGAGGGTGTATCCG" COMMENT "Antisense primer for PCR amplification of a region of Ple147 (pEMS1397)" COMMENT 0 (CStringList) 0) 1) 1 2 2 0 181) COMMENT " TTGGGGTGAGGGTGTATCCG" 1)) COMMENT #187=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Similarity: 100.0%" 1) COMMENT #188=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " Length: 20 Tm: 56.7 C GC: 60.0" 1) COMMENT #189=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT " dH: -157.5 kcal/mol dS: -401.4 cal/mol dG: -36.0 kcal/mol" COMMENT 1) COMMENT #190=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "Tm Difference: 0.2" 1) COMMENT #191=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "GC Difference: 0.9" 1))) COMMENT "#4: Product of length 677 (rating: 171)")))) COMMENT "PCR Analysis")))) COMMENT (CGraphView COMMENT (CWStyleSheet COMMENT (CObjectList COMMENT #192=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 COMMENT "@N (@S)" 0) COMMENT #193=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #194=(CWidgetStyle "Molecule Label 2" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 COMMENT "@L bp" 0) COMMENT #195=(CWidgetStyle "Molecule Label 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 COMMENT "@N" 0) COMMENT #196=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 COMMENT (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #197=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 COMMENT (LOGBRUSH 0 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8421376) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 COMMENT 5 "@N (@S)" 0) COMMENT #231=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 COMMENT "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 COMMENT (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 COMMENT (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 COMMENT -16454) 1 1 0 1 1 COMMENT (mapper: 24.2375 -14.5217 40.0378 40.0378 0.01 10 -1 18813 18813 1 0 0) COMMENT #232=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 1701672291 100) COMMENT (CObjectList COMMENT #233=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -883 100) COMMENT (CObjectList COMMENT #234=(CAxis COMMENT (CWideLine COMMENT (CWidget 0 (0 0) 1 2 0 0 #198# 41196972 0) COMMENT (LOGPEN 0 0 10079436) 2 (LOGBRUSH 0 13434879 0) 1 COMMENT 6.27452 6.27252 1 0.0214037) 0.0333449) COMMENT #235=(CLabel COMMENT (CWidget 1001 (0 0) 1 2 0 0 #195# 1129596743 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 58 22 0 0 400 0 0 0 0 3 2 1 34 "Verdana") COMMENT 1.74233 0.833333 0 "Ple147 (pEMS1397)" "@N" 1 0 COMMENT 0.871165 0 -10 11.4392 1.47361 Nil) COMMENT #236=(CLabel COMMENT (CWidget 1002 (0 0) 1 2 0 0 #194# 1128481607 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") COMMENT 1.74233 0.555556 0 "18813 bp" "@L bp" 16 0 -0.871165 COMMENT 0 -10.8 3.12205 0.949103 Nil)) (CObjectList)) COMMENT #237=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -1022 100) COMMENT (CObjectList COMMENT #238=(CGroupWidget COMMENT (CWidget 21 (7 21 0) 1 2 0 0 Nil -255 100) COMMENT (CObjectList COMMENT #239=(CScratch COMMENT (CWidget 0 (3 #5# 0) 1 2 0 0 #201# 1701 100) COMMENT (LOGPEN 0 8 13395507) 10 1 2.05305 1.9 COMMENT 0.082322 1) COMMENT #240=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #193# 1195852116 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "I-Sce 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(LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.835356 5.18214 COMMENT 5.42222 1 0.082322) 0.8 1.8 0) COMMENT #246=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #193# 20601756 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "EGFP (mutated stop TAA -> TTA)" "@N" 1 COMMENT 18.6598 1.74233 0 -10 15.0358 1.17389 #245#) COMMENT #247=(CScratch COMMENT (CWidget 0 (3 #9# 0) 1 2 0 0 #201# 26696480 COMMENT 100) (LOGPEN 0 8 13395507) 10 1 5.18214 1.9 COMMENT 0.082322 1) COMMENT #248=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #193# 393221 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "Tetracysteine residue" "@N" 1 16.3925 0 0 COMMENT -10 10.0405 1.17389 #247#) COMMENT #249=(CScratch COMMENT (CWidget 0 (3 #10# 0) 1 2 0 0 #201# 897 100) COMMENT (LOGPEN 0 8 13395507) 10 1 5.17013 1.9 COMMENT 0.082322 1) COMMENT #250=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #193# 1129595719 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "NLS" "@N" 1 COMMENT 12.7707 -1.74233 0 -10 1.99811 1.17389 #249#) COMMENT #251=(CScratch COMMENT (CWidget 0 (3 #11# 0) 1 2 0 0 #201# 897 100) COMMENT (LOGPEN 0 8 13395507) 10 1 5.14413 1.9 COMMENT 0.082322 1) COMMENT #252=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #193# 28214164 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "FRT" "@N" 1 COMMENT 12.4261 -3.48466 0 -10 1.92318 1.17389 #251#) COMMENT #253=(CScratch COMMENT (CWidget 0 (3 #12# 0) 1 2 0 0 #201# 28236624 COMMENT 100) (LOGPEN 0 8 13395507) 10 1 5.08177 1.9 COMMENT 0.082322 1) COMMENT #254=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #193# 1128482625 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "SV40 early polyA signal" "@N" 1 16.2285 COMMENT -5.22699 0 -10 11.0645 1.17389 #253#) COMMENT #255=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #13# 0) 1 2 0 0 #197# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.835356 5.44323 COMMENT 5.78602 1 0.082322) 0.8 1.8 0) COMMENT #256=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #193# 26741756 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "Prom region" COMMENT "@N" 1 12.6623 6.96932 0 -10 5.86945 1.17389 COMMENT #255#) COMMENT #257=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #16# 0) 1 2 0 0 #197# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.670712 5.44323 COMMENT 6.22717 1 0.082322) 0.8 1.8 0) COMMENT #258=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #193# 268634520 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "Ple147 (NTSR1-D)" "@N" 1 10.1324 10.454 0 COMMENT -10 8.06738 1.17389 #257#)) (CObjectList)) COMMENT #259=(CGroupWidget COMMENT (CWidget 22 (7 22 0) 1 2 0 0 Nil -116 100) COMMENT (CObjectList COMMENT #260=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #1# 0) 1 2 0 0 #209# 1321 100) COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 1 COMMENT 0.0020007 0.335784 1 0.082322) 0.8 1.8 0) COMMENT #261=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #193# 1195852116 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "AmpR" "@N" 1 COMMENT -4.09441 12.1963 0 -10 2.87229 1.17389 #260#) COMMENT #262=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #2# 0) 1 2 0 0 #209# 0 100) COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 0.835356 COMMENT 2.05505 2.53055 1 0.082322) 0.8 1.8 0) COMMENT #263=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #193# 21792600 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "AmpR" "@N" 1 COMMENT -10.7874 -8.71165 0 -10 2.87229 1.17389 #262#)) COMMENT (CObjectList)) COMMENT #264=(CGroupWidget COMMENT (CWidget 27 (7 27 0) 1 2 0 0 Nil -953 100) COMMENT (CObjectList COMMENT #265=(CScratch COMMENT (CWidget 0 (3 #14# 0) 1 2 0 0 #201# 27924948 COMMENT 100) (LOGPEN 0 8 13395507) 10 1 5.71399 1.9 COMMENT 0.082322 1) COMMENT #266=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #193# 2082602612 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "oEMS2607" "@N" COMMENT 1 10.8535 8.71165 0 -10 4.79547 1.17389 #265#) COMMENT #267=(CScratch COMMENT (CWidget 0 (3 #15# 0) 1 2 0 0 #201# 694 100) COMMENT (LOGPEN 0 8 13395507) 10 1 5.93307 1.9 COMMENT 0.082322 1) COMMENT #268=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #193# 2082602612 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "oEMS2790" "@N" COMMENT 1 7.02551 12.1963 0 -10 4.79547 1.17389 #267#)) COMMENT (CObjectList)) COMMENT #269=(CGroupWidget COMMENT (CWidget 86 (7 86 0) 1 2 0 0 Nil -534 100) COMMENT (CObjectList COMMENT #270=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #0# 0) 1 2 0 0 #197# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.835356 0.775604 COMMENT 2.04671 1 0.082322) 0.8 1.8 0) COMMENT #271=(CLabel (CWidget 0 (0 0) 1 2 0 0 #193# 5 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "5' arm of Hprt homology" "@N" 1 -16.8739 COMMENT 1.74233 0 -10 11.2144 1.17389 #270#) COMMENT #272=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #3# 0) 1 2 0 0 #197# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 2.52888 4.47022 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #273=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #193# 3670672 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "3' arm of Hprt homology" "@N" 1 11.4827 COMMENT -10.454 0 -10 11.2144 1.17389 #272#) COMMENT #274=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #4# 0) 1 2 0 0 #197# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.835356 4.47022 COMMENT 5.0631 1 0.082322) 0.8 1.8 0) COMMENT #275=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #193# 21645076 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "HPRT complementary sequence" "@N" 1 18.8104 COMMENT -6.96932 0 -10 14.9609 1.17389 #274#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #276=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -116 100) COMMENT (CObjectList) (CObjectList)) COMMENT #277=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -185 100) COMMENT (CObjectList) (CObjectList)) COMMENT #278=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -325 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 COMMENT (CObList #279=(AnlzFontBackColorItem 1461 1676 40304639) COMMENT #280=(AnlzFontBackColorItem 1697 1733 40304639) COMMENT #281=(AnlzFontBackColorItem 1677 1696 43633766) COMMENT #282=(AnlzFontBackColorItem 1754 1780 40304639) COMMENT #283=(AnlzFontBackColorItem 1734 1753 43633766) COMMENT #284=(AnlzFontBackColorItem 1801 2488 40304639) COMMENT #285=(AnlzFontBackColorItem 1781 1800 43633766) COMMENT #286=(AnlzFontBackColorItem 1233 1252 43633766) COMMENT #287=(AnlzFontBackColorItem 1320 1339 43633766) COMMENT #288=(AnlzFontBackColorItem 1020 1041 43633766))) (CObList) 1919252069 COMMENT (CStringList) 1114668146 1914717249 (CObList))) FEATURES Location/Qualifiers misc_recomb 12676..16487 /vntifkey="86" /label=5'\arm\of\Hprt\homology /note="The 5 HPRT probe anneals to genomic sequence ~560bp upstream of the BamHI site at 9263" misc_marker 17807..18807 /vntifkey="22" /label=AmpR misc_marker 11225..12650 /vntifkey="22" /label=AmpR misc_recomb 5408..11229 /vntifkey="86" /label=3'\arm\of\Hprt\homology /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3" misc_recomb 3630..5407 /vntifkey="86" /label=HPRT\complementary\sequence /note="Consists of human HPRT promoter, human Exon1, Intron1, Exon2, and part of Intron2" misc_feature 12657..12674 /vntifkey="21" /label=I-Sce\I misc_feature 2512..2545 /vntifkey="21" /label=FRT\(F5mutant) misc_feature 2546..2556 /vntifkey="21" /label=Kozak\consensus misc_feature 2553..3272 /vntifkey="21" /label=EGFP\(mutated\stop\TAA\->\TTA) misc_feature 3273..3308 /vntifkey="21" /label=Tetracysteine\residue misc_feature 3309..3381 /vntifkey="21" /label=NLS misc_feature 3387..3420 /vntifkey="21" /label=FRT misc_feature 3574..3624 /vntifkey="21" /label=SV40\early\polyA\signal misc_feature 139..2489 /vntifkey="21" /label=Ple147\(NTSR1-D) misc_feature 1462..2489 /vntifkey="21" /label=Prom\region primer 1678..1697 /vntifkey="27" /label=oEMS2607 primer 1021..1042 /vntifkey="27" /label=oEMS2790 /note="57.0" BASE COUNT 4848 a 4420 c 4465 g 5080 t ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctccta ggccggccac cctccgcttg cttttcctcg ggctctccct gcctgggcct 181 ggaggaccac agcgggcaca gggggcggct caggctgaga accaacggcg ctgacgtcgc 241 ctaaaggggg ttcccagctg gcagcctcca ggtgctggaa tgtttctcgg tgagaagccg 301 ccacgggagt ctgtggactt gtcacatcac ccgcaggcgt ggccctgagg ccacgtatcc 361 aagcagcagt gccgtgcata ttcatgtcgg cattggactt gcaaattctg agtgaagtaa 421 atgtcatcgg cagtgtgtag acgttctttc ggaagaagat ctgtcagaga aaatgcaagc 481 tgattaccag cctaacagag aaatgtgcat cccggtcccc gcagggccac cctggaggca 541 cctctgcacc tcccaggcac agctctggga aggctgaaaa ttagcaccaa ttagggaaga 601 atgaagctct tggttccctc ctgaagcact tggttgaagc ttgctgcgct cctttccggg 661 agcaaatgag catctctcgg gacagttgtg tttcccttgg gcagggagcc actggcagct 721 ccccagtggg aatcgtgcct tctagtccag atttccactt tggaaaggga aggttctaga 781 aagttctgga aagctcggct gtggctgact cctggcttag ggctcaggcc tgtctcacct 841 agcaatgcag ggtgagccat ggtgacgtgc acactaaaga aagacgctca gctccctcct 901 ctgccccgcc tggccgccag ctcagtgcca aaaccatggt tagggggtgg gaggccacgg 961 gtgcccttgg ggacctacca ggcctgcagg gtccttggca gaggggcagt gcctggcacc 1021 aggaggacac tgtgctggaa ggtcagcctg ttcccacagc cagggatgtg gggctcccag 1081 gtagccccat tcatagttat ttgaattgct gtgatggagg caggcggaag aggtgggact 1141 gcgagggaag gcaggggccc cccggggaga gagtgggtgc ctccttccct ttcctcaaag 1201 cccaacggcc ttgcagtgag actgccacta tacaacaaag agccagcacc gctgtgcacg 1261 ctggagtctc acagatgagc cgattatgtc tttgtgacaa acacattatt aggagtgaga 1321 tgaattcctt ctgtgggcgg ggatgattca gagagaggcg tcccagcccc aggaacagcc 1381 tccccgcaag gtggcctcag cctcccccat gctcccctgg aggccgctcc ctggcttggg 1441 gggtgggtaa cacgggagtc cggcacatcc cagaaatacg cattcacaga ccaggcacgc 1501 ccatgactgc ggccagattt taaaaggaat caccagaaat gtaatctcca gtggtcctag 1561 agccccctgg gcacccagct tctggcaccc tgtggacacc caggagtggg ggtcggccca 1621 accagctgcg cagccgccca gccctgccag cggggagctt cctccctccc tgccctccgg 1681 atacaccctc accccaactg agcgagccca gggttggggg aggtgcatag ttcccttcct 1741 agccggtgcg agaaattgcg gagaacaggg aagagggtgg caggaagggt ctggtgcacg 1801 atacccgccg cgcccgctcg gaatcctggt tccctggagc gctcacccca gcccgccctg 1861 ccccggggag cgcgaggggg cgacccgcca ggctgcaccc gggcggggcg gggatggcgg 1921 cgggggctgc ctccctcctc cgccgccacc ccccactgct gggcgcgccg cgcggccgcc 1981 ggctccgtgg agacgcgcag agccgggagg gcgcgggcgc ggcgggaggt gtgcggggct 2041 ggggtgcgcg gagagcgcga gggaggagat tctgagcgag ggaggggagc gcgcccgggc 2101 agcagtgcgt gcgctcctcc cggcgccaca agctcgcccc gcgcagcccg agccgggctg 2161 ggcgctgtcc tcgggggcct ggggaaccgc gcggtttgga gatcggaggc acctggaacc 2221 cgtggcaagc gccgagccgg gagacagccc gaggaaccac gggttctgga gctaggagcc 2281 ggaagctggg agtccggagg agagcggagc ccggagcccg gagcccgggg cggcgcgtct 2341 gggtctggcg cttcccgact ggacggcgcg cccgctggtc ttcgccacgc gccctcccct 2401 gggctcccgt tcatcggtcc ccgcctgaga cgcgcccact cctgcccgga cttccagccc 2461 cggaggcgcc ggacagagcc gcggactccg gccggccacg cgtggcgcgc cgaagttcct 2521 attcttcaaa aggtatagga acttccgcca ccatggtgag caagggcgag gagctgttca 2581 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg 2641 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca 2701 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc 2761 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc 2821 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc 2881 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg 2941 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca 3001 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc 3061 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg 3121 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca 3181 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga 3241 tcactctcgg catggacgag ctgtacaagt tatttctgaa ctgctgcccg ggctgctgca 3301 tggaaccgga tccaaaaaag aagagaaagg tagatccaaa aaagaagaga aaggtagatc 3361 caaaaaagaa gagaaaggta atataagaag ttcctattct ctagaaagta taggaacttc 3421 agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt 3481 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg 3541 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 3601 caaataaagc atttttttca ctgcgaattc tcgtaaaact cttcatggca gtagttatta 3661 ttctctctct ctctcttttt cttttttctt gagacaggat atttctctgt tgcccaggct 3721 ggagtgcagt ggcacagtct tggctcactg cagcctggac ctcctgggct caagccatcc 3781 tcccacctca gcctcccaag tagctggggc tacaggcaca tggccaccag gcccagataa 3841 tttttcattt ttgtagagac tgagtctcac catgttaccc aggtttatta ttctcatttt 3901 ttagatgaag agactgaggt ccagagaagc tcaatgactt gcctagtttt acaaatctcc 3961 tgccatcaca tacccctcag cgtccttaat aagagggagg ccaccaacta tgtgctgggc 4021 actgtggtgg atgctggagc tatagggttg agtatataag aaatggtgtt gctggagcaa 4081 ctgttgcttg cttacctgac ctatctgaga attaattagc aggggaacat atttttgttt 4141 tcagattcaa tataagaact tgtgtgggca aaaataaaga tcagtagtaa taacagtagt 4201 tcccatttgc tgactgtact gtcctaagtg catatatata tacatacaca cacgcatacc 4261 tatactcctc taatactcaa aatgatcctg tttatgtatt gttaatatgc tcattttatt 4321 tttaaatttt tatttatttt tatttttatt tatttttgag acggagtctc attctgtcgc 4381 ggaggctgaa gtgcagtggt gcgatctcag ctcagtgcga cctccgcctc ccgggttcaa 4441 gtgattctcc tgcctcagcc tccggactag ctgggattac aggcgcccgc ctccacgccc 4501 agctaatttt tgtattttta gtagagatgg ggtttcgcca tgttggccag gctggtctcg 4561 tactcctgac cttgagtgat ccacctgcct cggcctccca aagtgctggg attacaggca 4621 tgagccaccg cgccgggcta atatgctcat tttagtgagg caaaaataga ggctcagagt 4681 ctgatttgta caaaactaca gagcagttaa gtgtcctctc agatgtgtac cctgatctgg 4741 gtgactctag gactctaggt ctcaactgtt acaaccagtt aagggtttgg ggaagcactg 4801 ggccaagagt caggaaaatg gaagccacag gtagtgcaag gtcttgggaa tgggacgtct 4861 ggtccaagga ttcacgcgat gactggaacc cgaagagccg gggcccggtt tacggccgcc 4921 atgaagcaac gcgcgccggt aggtttggga atcagggagc cctctgaata ggagactgag 4981 ttgggaggga aaggggcttc gctgggggag cctcggcttc ttctgggaga aaattcccac 5041 ggctacctag tgagcctgca aactggtagg cgccggcgta ggcgcgcggg cggggccggg 5101 ggcggggcct gcggggcgtg gcggggcggg cagagggcgg ggcctgcttc tcctcagctt 5161 caggcggctg cgacgagccc tcaggcgaac ctctcggctt tcccgcgcgg cgccgcctct 5221 tgctgcgcct ccgcctcctc ctctgctccg ccaccggctt cctcctcctg agcagtcagc 5281 ccgcgcgccg gccggctccg ttatggcgac ccgcagccct ggcgtcgtgg tgagcagctc 5341 ggcctgccgg ccctggccgg ttcaggccca cgcggcaggt ggcggccggg ccctgaggcg 5401 cgggatcgat ccatgacacc cccccgcccc ccatatctca ctataattca ctgtcaaatg 5461 gcttttatga tttggagttt ggggatactt ttaaagacag ggtcttggta gctcctagat 5521 ggcttgggat tcacttagta acccttgctg gcctaaaatt caggaacttc tacctcatct 5581 tcccaagtac tgggttagta gggatgtcca gcatatttag cttgctttaa tgattttgct 5641 gtactttttt ctgtgtgtac aggtgcatgt atatttataa aactaacata atatatttta 5701 cccccaaagt accataaccc taattaatgc tattaaatgt tttgtatgct gggctctggg 5761 catactaata taaccaatat tatacaatgc aaaacaattt tggaatatct tctgtaaaat 5821 tatcttatta gtaagttgta accatctcag acttctaagt ttgagtttgt aaaggatcag 5881 ttttttttgg tgacctacac acacacacac acacacacac acacacacac acacaggggg 5941 gtggattctg agaatttaga gaatttagat ggttaatacc agtttttatt tttattctgt 6001 gtgatattta tttattaaga tttatttatt tattacatgt aggtacactg tatctgtctt 6061 cagacacacc agaagagggc atcagatctc gttacagatg gttgtgagtc accatgtggt 6121 tgctgggatt tgaactcagg acctctggaa gaacagtcag tgctcttaac cgttgagcca 6181 tctcaccagc cccgtgatat tttatttaac ttactagaat atactgtgtt aaaacatatt 6241 ttaggggatt ggggagatgg cccattctgt aaagtgtctg ccatgtcatc ttcaggacct 6301 gcgtttgtaa ccccagcatg gagaagacag agacagggag acccctgggc ttcctggcaa 6361 gctagactag caaatttggt taactaggtt caatgagaga ctcagtctca aaacataaga 6421 agtgaatttt ttagacccaa tttcaatctc tgaactccat acgtatgcac atgtacctgc 6481 atataaatat atatacacac ccataaacac atgcacacac aaaaaatggt tcaaaagttc 6541 ttaaaatgta taagagtaga aagtgtagag ccgggagtgg tggcgcacgc ctttaatccc 6601 agcacttggg agcagaggca ggtggatttc tgagttcgag gccagcctgg tctacagagt 6661 gagttccagg acagccagag ctatacagag aaatcctgcc tcaaaaaacc aaaaataaag 6721 tgtatatact aatctttcat gcctaaaata tttatttttg ccacattttt catacctctt 6781 taatattttt ctttttctct ttgtgcttca aaggaaattt tgtgtcatgt tttacttttc 6841 attcttgact ctattcagca gtaagacgca gcatggcttt cttagagaag tataatctat 6901 tacaaaacgg taacatttaa ctgctctaca taccctcttg tagctaaatt cctctgttaa 6961 actaagactt attatctaaa cgtaattaaa tgttgatatc ttataatgct ccactttgaa 7021 acagctgtct ttcttttgca gattagcgat gatgaaccag gttatgacct agatttgttt 7081 tgtataccta atcattatgc cgaggatttg gaaaaagtgt ttattcctca tggactgatt 7141 atggacaggt tagtaagacc tcgattgaag ttatttttag agggtgtgtg tgtggtgtgt 7201 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtaagaga gagaggagag gcatgtgtga 7261 tatatatgtg aagtgtattg catgtacatg gaatccagag aaggacatta ggtgtcctac 7321 tctgttgctc tcttattccc ttgaatcagt gtctcttgct gaacctgggg catacatact 7381 gatttggcta ggctgcctgg ctagcaagac ccagggatct gcttgtctct gccccccagt 7441 gctgacgtta caagcaggtg caaccacatc tggtttttat atgggtactg ggggatctga 7501 acttagggcc tgatgcgtgc ccaagtgctc tcacccgcca agccacctct ccagccaaag 7561 ggtttttttt cttgtattaa ttctaactta gcaactgttg agtagttgat gtttgaatat 7621 aagctaagcc aacttattag aataaatgtt ttctattttt tacttgtatg tgtgagtgcc 7681 tgcatgtatg tatgtgaagc atatgtattc caggatgtca gaattgtgat ggattccctg 7741 gaactggagt tacagaccat tgtgaaccac tgtgtatggg tgctgggaac tgaactttag 7801 gtcctctgca aaaacagtaa ggatcttaac tgataaacta tctttctagc cctaaggctt 7861 tctactttta aagttcgttt ctctttctct ctctctctct ctctctctct ctctctctcc 7921 ctctccctcc ctcctcctcc tcctcctctt tcccccctct cgcctcctct ttctctctcc 7981 cctccctctc tctctctcac acacacacac agacagagag aggagagaga aaggaagcct 8041 tactaattaa ttacctagtt ttagacataa ctagtgggaa tccatagtaa gtaaatcaac 8101 attcttgaag agaggttaga agaattggct ctcgttgtcc atcgaggctg tataagatta 8161 ttcttctgtt ttctcagctt accatacata ttttctacgt agacaatacc aattcaaata 8221 cactgtacgt aaagtatatg cgcacacaca cacacacaca cacacacaca cacacacaag 8281 ttacatattg tcatatagta ctaaaattta tggacttttt catattaata catctagagc 8341 tatcatgttt tttatctttt aaaaattact ttacggggct acagagatgt ctcagcagtt 8401 aggagcactg actgctcttc cagaggtcct gagttcaatt cccagcaacc acatggtggc 8461 tcacaaccat ctgtaatggg atctgatcca ctcttatggt gtgtctgaag acagtgacag 8521 tgtactcata tacatgaaat aaatctttaa aaaattactt taccatcagg gtatatatat 8581 atatatatat gtgtgtgtgt gtgtgtgtgt gtatgaagta tttgctattt gagagggtct 8641 cactgtttag ctcagtctgg cttggaacgt tctgtatagc ccagactggc ctcaactcag 8701 cagtccccct gaaaggctct catatgtgga gattacaggt atgtgctact gtgcccagcc 8761 ctacatgtta ctttgttttt gaaatagggt atcaaatagc ttagactggc ttctaagtca 8821 gtgtgtagct aaggatggtc tgagcttctg aacctgctac ttcaactcct ggtgtgccat 8881 catggccagt ttgcacagtt gtgagagtag aactcaggac ctcctatgtg ttactagaca 8941 tgttctctac cagctgagct acatcccaat tcgcctacat ttacttttat tttttaaaag 9001 atttatttat gtatacagca ctctgcctgc atgtacatct gcatgccaga agagggcacc 9061 agatctcatc atacatagtt gtgagccacc atgtggttgc tggggattga actcagggcc 9121 tctggaagag cagtcagtgc tcttaacctc tgagccatct ctgtagcccc ctacatttat 9181 tttaaagatt ataggctatt ctattcatag atgtgccata actgatttaa ccagatcttc 9241 attattttgt acttagtaga gtatattaga gggaatgaat cttaaataca cattttgaca 9301 cttgctcagc tttagacaag taaaaatcac tggtcaagga aatggtgctt agtggatgct 9361 tctgaactgt caaattgtcc tccaacaagt tacatcattt tatacttcca acgttatgtg 9421 agagttcatt tttctttagg ctcttctagt attttatatt tgagaaataa aatttaacca 9481 aaatttgcca atctagtaag cttgttttta tttgtatctt taaattttat gatagatgtt 9541 atagtgtact ctcctctccc ctcccctccc ctctcctcct cagcaggatc ttgttatgta 9601 gccctaaggg atctaatact tactctgtag tccaggctgt ccttcagctc atgagacaca 9661 ccatcatgct tggcatgttt cttgttctga aaggtatctg gttttaattt tggttgagct 9721 ggtgtgttgg tgtatatctg taatctcagc acaggcaagt agagacaagg attatgatta 9781 cagtccagcc tgggctattt agagggattc tctttaatct ttcctcatgc cccaaaatct 9841 tacctttggt atatgaaaaa tagtctccac ttctgcaaaa tattgcttta tgaagtaaga 9901 attaattccc ttcatagaga caaggaatgt gtcctgtaaa agtttaatgt gtaagaagta 9961 tttgttataa aagataaata ttcagaatct tctttttaat tcctgatttt atttctatag 10021 gactgaaaga cttgctcgag atgtcatgaa ggagatggga ggccatcaca ttgtggccct 10081 ctgtgtgctc aaggggggct ataagttctt tgctgacctg ctggattaca ttaaagcact 10141 gaatagaaat agtgatagat ccattcctat gactgtagat tttatcagac tgaagagcta 10201 ctgtgtaagt ataattaact tataattaaa aaaatagggc cattctagtt ttatctatat 10261 tttttttaaa cttgtgcaaa ctatgctaca taatcaactt acttgattat gtaatatata 10321 tagtaatagt tggaattact attattacat atttattttt ctgtgctggg gtagaaccca 10381 gggaactaag tactgtaata accgagcttc attcatccta gtcattttta attttttttt 10441 ttttttttgt tttttcgaga caaggtttct ctgtgtagcc ctggctgtcc tggaactcac 10501 tctgtagacc aggctggcct cgaactcaga aatctgcctg cctctgcctc ccagtgctga 10561 gattaatgac gtgcaccacc actgcctggc atttttttaa agttttaatt tgagaccctt 10621 tctcactaac ttgctcacac tatcctcgaa ctcattacgt gggctggtct agaaatttca 10681 gtcaacttgc ctcagcttct caagtagctg ggattacagg caggtgctac cacttctggc 10741 catgatcatt gttgttgttg ctattattat tattattatt tcactgatac ttcatatcac 10801 aacacagaat agtaactgta gttcatgacc aacaaaaaag ttgtacagtt tctctgttgt 10861 cttattttga tctggatttt gatttgtttc atggcactgg gcaggctaat ctcaaactgc 10921 atggctcaaa tagtccttct gtcttaatct cccatgttct ggaagtatag gtatcactac 10981 acctagctgt attttcatat tttaaaaaaa acgcctaaga attgctattg aatgaggcgg 11041 agagatggct cagcagtcac aagtacttgt agaggactgg ggtttggtta ctagcacctc 11101 aaagatggct tacaattatc tgtagctcca attccagagg atctgtgtgg taaagctgca 11161 tgtatatggg catgcaggta aaactgctaa acatataaaa atagctcagt cttaagagaa 11221 ttggtcgact ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 11281 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 11341 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 11401 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac 11461 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc 11521 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt 11581 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct 11641 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga 11701 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag 11761 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca 11821 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga 11881 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag 11941 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc 12001 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa 12061 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt 12121 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg 12181 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt 12241 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg 12301 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat 12361 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact 12421 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa 12481 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 12541 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 12601 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag gtcgagtagg 12661 gataacaggg taatggatcc tcgttgggca cctgcactca tgtacgcagg cccacatata 12721 gatgtacaca cgcatatcat tcaaaataga aattatttat acaagtagtg cgttggagag 12781 ctggctcagg agttgttaat gcttgctacc cttgcaaagg acccaagttc aatttccagc 12841 acctgtgttg ggcatctcac aaccacatgt aaatctagtt tcaggggatt tcacccctct 12901 tctgaactct tcaggcactt atacccacaa atggacatct ccacatagag acacatactt 12961 ttaaaaaagt aatctagggt ctagagaaat ggttatagac cacggactgg gatcagttcc 13021 cagaagccac atcctgtggc tcacaattgc ctgtgaatca agttctagat ctggtggcct 13081 cgtctagcct gcagtcatca acaaacacat gcacacatcc atacacacat aatttaaaag 13141 aaagcgaaag taacctataa tgctaaacaa agcacattct cagtgtagac tgcagtggtg 13201 atgaaccttg aaaatatatt aatagaagcc agacacgtaa gtcacaaatg gtataactca 13261 atttatatga aattcccaga gagtccaaag ttaaaaaaaa caaaaaggca gattattggt 13321 tgtcagtagc tacagagatg ggacattgaa gatattaata ggtatagggt tgaggattca 13381 tggcctttcc ctcttccatg ttagcatgtc tattgttgtt aaccttattc aactcatgtt 13441 agggcaatca tattgatgag acttaatgag tatagcttga cattcctaga agacagagtc 13501 tcacatcaga ctctctgatc ctctgactct tagaatcttt caacaccctc ttctgaaatg 13561 atctctgagc ctttggttgt gactgaatta tgagaaagaa ttttatgttg aatgaatttt 13621 acttcagcat ttttacaaaa ctgaagctgg ggatggcgtt tcatgcgtgc agtccctacc 13681 atttgcctat aagcctggag gaggcagaag tagaattagg cttttcaggg acatcttcag 13741 ctgcatcatg agcttgagga caacttgagc tacataagat tcccatcccc acccccacac 13801 acacacacac atctagtatg tacctagtat gcagtctttt catatgttta aaatgtagaa 13861 aggtatatgt gaagtctccc ttttccccac aacttagtcc ctccaaagta ttgcatcagt 13921 ttccttgtac atgattttat gcatatataa gatacactat ttctctctct ctctctctcc 13981 ttccctcctt ccctccaacc tctgggtatg agttgagggt tatgtatgtt tcaagttgta 14041 ataagaattg ccactcaaac atttcatccc attttaagtt aagtttgtct gattttccac 14101 tttagaaggg cagccatgat ggctcatgcc tataatccca tcctagcact tgtaagccta 14161 atacataaga ctggaggatt gtcatcagct tgaggctagc ctggtctacc taatgagttc 14221 cagccagcca agagtagaat ataagaccct gtctccaaaa aatgtaccaa aggggctgga 14281 ggggtaactc agtggttaag aacagtagct gctcttccag aggacctggg ttcaattccc 14341 agcacccaca tggcagcttg caactatctg taactgtcca gctttgggat atccgacacc 14401 attataccaa ttacataaaa tgaaaaaatt gtaaaatgtg ctaaaatgag tggggagggg 14461 gagcatggtg tggttctgtg ggcttgggat tttgccatga atccatgact atatctcagc 14521 aatatgtgga ttatttctgg aaacctgtgg ggcatgggtg tatgggaaga ggggagagag 14581 agagagcccg catccagcca gagttcctgt gctctgggca ggcggacgtg ggaggactgc 14641 tggacgattt ccacttggcc ctgggtgggc atctggctgt gtgaagccac tgaccccact 14701 cagcgggggg tagataaggg gcatttccca taccaggttc tcagactcca gcattccacg 14761 ctggatacag cagaagagct gagaacagaa taggggcccc agggagacac tctcgtccca 14821 gagataaaga aggcagaggg agagagattc ctacgcaggc gagaggcctt agtctgggcc 14881 ttgatgaaca gagacggtct atggttttag agctttattg tagaaaggca gggggaaaga 14941 gagaaggtag aaagagagag accagccatg gccaagagga gagaaggggg gaaaggagag 15001 agagaagaaa ggctagagag taagaaaggt taaagagaca gaagtaaaag cttagagtaa 15061 gagagagaga gagagcaaga gggagagtaa gaaaagtaaa agcttaagag agcgaggagg 15121 ggccagcccc tctaatagtg ggcttgttat cttgttgttg ctaggtaact gtgaagcgtc 15181 tagcctgaag gccagaagct tgggacattg tctatgtgac tgaaagccat gagcctctac 15241 tgtgggggct gtggaggtgg taacttggac aggagccagg ggttccagga gacatgaggg 15301 aatgccttct gtcccatgta ggtaggaatt accaccaccg gctttcaccg tagttcactg 15361 gagactgttt gtacatagcc cattgcccca cagaaaccag gcactcactg tttcatttgc 15421 ataagaatta gaaggcttta ttttggctgg gattggtgac ttgccccaga aattccagct 15481 tttgggaagc cgaatcctgg agaaccagga gttcaaggtc atcctcaggt acctcatttg 15541 ttcaagacca gcctgtgcta ccatgagacc ttgtccctac tcccctcaac cccaaaacac 15601 gctgttttca ttttataggt tgtactaacc atggaggatg gtcatttgcc tttctcttga 15661 tttcagaagc gtagcgcggg ctttgcacca tgatctagtg tttccagata ggcttgcctc 15721 tccacagcca ggcttatcct gcttcagtcc catgtttggc aagggcaaca tatgactgaa 15781 gaacttgcat agccttatgg tgtatcatga ctcttagccc cagtttataa ctgtctaaca 15841 gctggtggct tttgtgaaaa aggaaagcat catttgaact tttaactttt cagctgtttg 15901 ttttgacttt gcataaatac tgagactagc taggaaatga gcttttgttt catttgctaa 15961 aaatgatttt tttttcatga ggcaatttca agcaatttga atttagcaat ctctacttgt 16021 ctgaaaagaa gttattaatt aaaaaaaaac aaacaaacag gaacctatta tgctggctag 16081 tcacatgaaa gaatccaaat gaacacagtc aaggctgaat caacacatga aattattttc 16141 taattcccca aattacattt atgctaatta gtatgtagta aaacatttta attgtttcca 16201 atgttaaaca ttacagaaat aattttgatg ttttcctaat aacccagcct ttgatcatgt 16261 ttgcatttat ttgattgttg tcagtttatt aaataattaa gtaagagcaa caagataaaa 16321 tgacttcatg agagtccttt tctgtaactt agaacgtcag tagtcatagg aactgcggtc 16381 gttcccttac ctttcagaag taggctaaag agttgaacgc aaaggtggtt tttcgaatct 16441 tcctgatcct taagccccca ctaggtttat attctatatt tgatatcgga ttcgaacccc 16501 tcgagcttag gatccggaac ccttaatata actaagttat taggtccctc gaagaggttc 16561 actagtactg gccattgcgg ccgcttcgag gctgcctcgc gcgtttcggt gatgacggtg 16621 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg 16681 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca 16741 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca 16801 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa 16861 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 16921 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 16981 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 17041 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 17101 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 17161 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 17221 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 17281 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 17341 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 17401 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 17461 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 17521 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 17581 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 17641 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 17701 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 17761 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 17821 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 17881 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 17941 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 18001 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 18061 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 18121 tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 18181 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 18241 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 18301 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 18361 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 18421 ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat 18481 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 18541 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 18601 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 18661 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 18721 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 18781 gcgcacattt ccccgaaaag tgccacctga cgg //