LOCUS       pAAVscCBPITuDmiR        5368 bp    DNA     circular     22-FEB-2012
SOURCE      
  ORGANISM  
COMMENT       #1: FRAGMENT of <a href="vnti:dnarna/pENN%20AAVsc%20F?sel=165-152">pENN AAVsc F</a> 
                parent position: from 165 to 152 (+ fill of 4)
                original length: 3320
                molecule position: from 836 to 146
                Left Terminus
                    XbaI site #1
                Right Terminus
                    MluI site #1
                      Completely filled in
              #2: FRAGMENT of <a href="vnti:dnarna/pENN%20AAV%20CB6%20%28p1017%29?sel=203-891">pENN AAV CB6 (p1017)</a> 
                parent position: from 203 to 891
                original length: 689
                molecule position: from 147 to 835
                Left Terminus
                    SalI site #1
                      Completely filled in
                Right Terminus
                    XbaI site #2
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|618502693|
COMMENT     VNTDBDATE|618503633|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAVscCBPITuDmiR122Gpluc7x122BT|
COMMENT     VNTAUTHORNAME|JunXie|
COMMENT     VNTAUTHORTEL|508-856-3316|
COMMENT     VNTAUTHORFAX|508-856-1552|
COMMENT     VNTAUTHOREML|jun.xie@umassmed.edu|
COMMENT     VNTAUTHORAD1|University of Massachusetts Medical School|
COMMENT     VNTAUTHORAD2|381 Plantation St., Biotech 5, Ste. 250|
COMMENT     VNTAUTHORAD3|Worcester, MA 01605|
COMMENT     VNTOAUTHORNAME|Gao|
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FEATURES             Location/Qualifiers
     CDS             3310..4170
                     /vntifkey="4"
                     /label=Amp-R
     repeat_region   complement(2418..2547)
                     /vntifkey="34"
                     /label=3'\ITR
     rep_origin      complement(2724..3179)
                     /vntifkey="33"
                     /label=f1\ori
     repeat_region   1..105
                     /vntifkey="34"
                     /label=ITR
     misc_feature    1..28
                     /vntifkey="21"
                     /label=repeat\FOR
     misc_feature    complement(68..95)
                     /vntifkey="21"
                     /label=repeat\REV
     misc_feature    4341..4929
                     /vntifkey="21"
                     /label=Origin
     misc_feature    183..468
                     /vntifkey="21"
                     /label=CMV\Ehancer
     promoter        472..736
                     /vntifkey="29"
                     /label=CB\Promoter
     misc_feature    636..642
                     /vntifkey="21"
                     /label=CAAT\box
     misc_feature    699..706
                     /vntifkey="21"
                     /label=TATA\box
     intron          771..1407
                     /vntifkey="15"
                     /label=intron
     misc_feature    828..1288
                     /vntifkey="21"
                     /label=TuD\122
     polyA_signal    2182..2377
                     /vntifkey="25"
                     /label=BGH\3'UTR+PolyA+GRE\(partial)
     CDS             1439..1993
                     /vntifkey="4"
                     /label=Guassia
     repeat_unit     2026..2175
                     /vntifkey="35"
                     /label=7xmiR122BT
BASE COUNT     1310 a      1404 c      1381 g      1273 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtgtagcc atgctctagg 
      121 aagatcaatt cggtacaatt cacgcgtcga cattgattat tgactctggt cgttacataa 
      181 cttacggtaa atggcccgcc tggctgaccg cccaacgacc ccgcccattg acgtcaataa 
      241 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt 
      301 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc 
      361 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat 
      421 gggactttcc tacttggcag tacatctact cgaggccacg ttctgcttca ctctccccat 
      481 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc 
      541 gatgggggcg gggggggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg 
      601 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa 
      661 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc 
      721 gggcgggagc gggatcagcc accgcggtgg cggccctaga gtcgatcgag gaactgaaaa 
      781 accagaaagt taactggtaa gtttagtctt tttgtctttt atttcagccc cagtggaaag 
      841 acgcgcaggc aaaacgcacc acgtgacgga gcgtgaccgc gcgccgagcg cgcgccaagg 
      901 tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata cgatacaagg 
      961 ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta gtacaaaata 
     1021 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa 
     1081 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttgggt ttatatatct 
     1141 tgtggaaagg acgcgggatc cgacggcgct aggatcatca accaaacacc attgatcttc 
     1201 acactccaca agtattctgg tcacagaata caaccaaaca ccattgatct tcacactcca 
     1261 caagatgatc ctagcgccgt cttttttaag ctgtcccgga tccggtggtg gtgcaaatca 
     1321 aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa gtgttacttc 
     1381 tgctctaaaa gctgcggaat tgtacccgcg gccgatccac cggtcgccac catctagcat 
     1441 gggagtcaaa gttctgtttg ccctgatctg catcgctgtg gccgaggcca agcccaccga 
     1501 gaacaacgaa gacttcaaca tcgtggccgt ggccagcaac ttcgcgacca cggatctcga 
     1561 tgctgaccgc gggaagttgc ccggcaagaa gctgccgctg gaggtgctca aagagatgga 
     1621 agccaatgcc cggaaagctg gctgcaccag gggctgtctg atctgcctgt cccacatcaa 
     1681 gtgcacgccc aagatgaaga agttcatccc aggacgctgc cacacctacg aaggcgacaa 
     1741 agagtccgca cagggcggca taggcgaggc gatcgtcgac attcctgaga ttcctgggtt 
     1801 caaggacttg gagcccatgg agcagttcat cgcacaggtc gatctgtgtg tggactgcac 
     1861 aactggctgc ctcaaagggc ttgccaacgt gcagtgttct gacctgctca agaagtggct 
     1921 gccgcaacgc tgtgcgacct ttgccagcaa gatccagggc caggtggaca agatcaaggg 
     1981 ggccggtggt gactagctcg acgctgatcg atccatcgcc gtgtaacaaa caccatacaa 
     2041 cactccacaa acaccataca acactccaca aacaccatac aacactccac aaacaccata 
     2101 caacactcca caaacaccat acaacactcc acaaacacca tacaacactc cacaaacacc 
     2161 atacaacact ccaggatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg 
     2221 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata 
     2281 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt 
     2341 ggggcaggac agcaaggggg aggattggga agacaattag gtagataagt agcatggcgg 
     2401 gttaatcatt aactacaagg aacccctagt gatggagttg gccactccct ctctgcgcgc 
     2461 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc 
     2521 ggcctcagtg agcgagcgag cgcgcagcct taattaacct aattcactgg ccgtcgtttt 
     2581 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc 
     2641 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt 
     2701 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 
     2761 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 
     2821 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 
     2881 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 
     2941 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 
     3001 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 
     3061 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 
     3121 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta 
     3181 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 
     3241 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 
     3301 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 
     3361 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 
     3421 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 
     3481 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 
     3541 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 
     3601 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 
     3661 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 
     3721 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 
     3781 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 
     3841 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 
     3901 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 
     3961 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 
     4021 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 
     4081 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 
     4141 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 
     4201 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 
     4261 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 
     4321 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 
     4381 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 
     4441 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag 
     4501 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 
     4561 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 
     4621 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 
     4681 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 
     4741 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 
     4801 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 
     4861 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 
     4921 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 
     4981 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt 
     5041 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag 
     5101 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 
     5161 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 
     5221 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg 
     5281 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac 
     5341 gccagattta attaaggcct taattagg 
//
