LOCUS       Ple176\(pEMS1088       20676 bp    DNA     circular     23-JAN-2009
SOURCE      
  ORGANISM  
COMMENT     PCR genotyping:
            Sense primer: oEMS2406  5'- CATGAGCAAAAGAAGATGGGACAGTm= 56.7 Deg C
            Antisense primer: oEMS2367  5'- GTGGAAGGAAAAGCGGCAAG-3' Tm=56.2 Deg C
            
            Cycling conditions:
            Step 1: 94 Deg C for 3 min
            Step 2: 94 Deg C for 1 min
            Step 3: 61 Deg C for 1 min
            Step 4: 72 Deg C for 45 sec
            Step 5: repeat Step 2-4 34 times
            Step 6: 72 Deg C for 5 min
            
            548 bp product
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|507991750|
COMMENT     VNTDBDATE|507991750|
COMMENT     LSOWNER|
COMMENT     VNTNAME|Ple176 (pEMS1088)|
COMMENT     VNTAUTHORNAME|Russell Bonaguro|
COMMENT     VNTOAUTHORNAME|Demo User|
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COMMENT                            0.871165 0 -10 11.4392 1.47361 Nil) 
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COMMENT                                   -10 12.7629 1.17389 #158#) 
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COMMENT                                   "5' homology sequence" "@N" 1 -16.1576 
COMMENT                                   3.48466 0 -10 10.4901 1.17389 #172#) 
COMMENT                             #174=(CScratch 
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COMMENT                             #189=(CWideArrow 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "oEMS2367" "@N" 
COMMENT                                   1 13.126 5.22699 0 -10 4.79547 1.17389 #192#) 
COMMENT                             #194=(CScratch 
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COMMENT               #196=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -116 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #197=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -185 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #198=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -743 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 
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COMMENT         #200=(AnlzFontBackColorItem 1743 2506 40304639) 
COMMENT         #201=(AnlzFontBackColorItem 2788 3034 50318387) 
COMMENT         #202=(AnlzFontBackColorItem 3055 3368 50318387) 
COMMENT         #203=(AnlzFontBackColorItem 3035 3054 43633766) 
COMMENT         #204=(AnlzFontBackColorItem 2531 2787 40304639) 
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FEATURES             Location/Qualifiers
     misc_feature    3378..3411
                     /vntifkey="21"
                     /label=MFRT\(F5\mutant)
     misc_feature    3412..3423
                     /vntifkey="21"
                     /label=Kozak\consensus\sequence
     misc_feature    3424..4140
                     /vntifkey="21"
                     /label=EGFP
     misc_feature    4141..5166
                     /vntifkey="21"
                     /label=Cre
     misc_feature    5167..5239
                     /vntifkey="21"
                     /label=NLS
     misc_marker     5243..5276
                     /vntifkey="22"
                     /label=MFRT
     misc_feature    5430..5480
                     /vntifkey="21"
                     /label=SV40\early\polyA\signal
     misc_feature    5486..9439
                     /vntifkey="21"
                     /label=HPRT\complementary\sequence
                     /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2"
     misc_feature    9440..13085
                     /vntifkey="21"
                     /label=3'\homology\sequence
                     /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3"
     misc_feature    14539..18350
                     /vntifkey="21"
                     /label=5'\homology\sequence
     misc_marker     13087..14513
                     /vntifkey="22"
                     /label=AmpR
     misc_marker     complement(19670..20670)
                     /vntifkey="22"
                     /label=AmpR
     misc_feature    14520..14537
                     /vntifkey="21"
                     /label=I-Sce\I
     misc_feature    139..3369
                     /vntifkey="21"
                     /label=Ple176\(RGS16-B)
                     /note="1302"
     misc_feature    139..1743
                     /vntifkey="21"
                     /label=Region\1
     misc_feature    1744..2788
                     /vntifkey="21"
                     /label=Region\2
     misc_feature    2789..3369
                     /vntifkey="21"
                     /label=Prom\region
     primer          3036..3055
                     /vntifkey="27"
                     /label=oEMS2367
     primer          2508..2531
                     /vntifkey="27"
                     /label=oEMS2406
BASE COUNT     5405 a      4742 c      4862 g      5667 t 
ORIGIN
        1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 
       61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 
      121 cgagctccta ggccggccgg aagtgtgcac accagttgtt agtgactatc attgtgttta 
      181 ctgcagccag ggtgtttggg acgcctgggg acttggatcc agaaagttcc cctgtggtca 
      241 catttggagc agcactacag ctagatggct ggcggaggag gtgtggctca cccaggctca 
      301 gtcacacagc tcaccgcgtg tgcccccctg ctcaaatgtg tggggcctat tagggagagc 
      361 tcatttctat ctctgcaaaa acatcatgca ccccctgctg tgctcttctc tctgtccacc 
      421 tgggcctgtt tctaggacag ctgctgtgtc aggggctgag ggcctgggag cttgtgaaag 
      481 gaccacctgt tcccattgct ttctccggcc ctcctccccc gccgccactc tgccggccca 
      541 gcctcccagc tgctcacaca tcacaccacg tttctccctc tgctcaggtg ttgtgagcac 
      601 attgtgagag gcatgatccc ttccaacttg tcgccacatt acagctagtg ttggtgcttt 
      661 tgttgagcct cccctgcttt cctataaaat ggtttttctt ctgtgcccct gcaccagact 
      721 gccccacccc caccccctac tctccctgtt gacatgggga ctcctggaag gtgactcaaa 
      781 ggaggaggag gtaccccaca gaaagtaggt ccttaactcc caggagatga caaagcccct 
      841 cccatgcctc tgacctgaca tctctgcacg tgtgtgtggt attcacatgt tggggcttgc 
      901 tgttcctggg gtccttatgt ctcatctgtg gcctaggtaa gcacctagaa atttctgact 
      961 agaacaagga ggagaaatgg cctgaagtag cagccaaggt ccagggggag agatgctcag 
     1021 aggctaggga acatggggac aaggcattga atagccctgg ggttggacct tgtggataga 
     1081 actgggtttc tagcctggca ctcccattca ctaggtgtgt cactgtgggg agattactta 
     1141 cgcaccgatg tctgtttccc cttctgtaca ggtggcatct gtaaggccct gagtccagcg 
     1201 ttggatcatg gagggcccta aaaatggtag tttccttttc tctaagacag ggcctggagg 
     1261 aagaccagac atcaacctcc caagctggct gaaatcgtct tactgtctga cacagagccg 
     1321 ggttctccct agtacatgct gctctggaat acctaggaga ggaagaggaa ggaggcaagg 
     1381 taagctgggg gcttttcaca ctaaaggcct acagaggggt aggtggagtc ggcataggtg 
     1441 ccatgtgtgt ttggtgtctt gcttccgact ctagctgggc tctggagcca ctgatgtctg 
     1501 cttgaccctt ccctgatttt gctccagttt gctctgcact gggagactgc aagccacagg 
     1561 gggaggggag tgtaagaggg ctgcagagta ataagtttca ctgcataatt cagccctcct 
     1621 gggacccgtt tccagaaagc tgactggctg aagtttagct gagaatgcct gcaatctgga 
     1681 ggatctgggg caggcagggt tgacagttaa tctccaagga tattcaccat gtttggagtg 
     1741 ggaaaaggac taggggacgg gaaatataaa catcaccttt cagtcatttg caaatcccag 
     1801 gcctcacgtt tcccccctct aatctttcat tctgactggc ttctcagggt cttggaaaat 
     1861 cattacagta aaatgcagat tctaataagc cttgtttcta agtcttagtg aatggaaatg 
     1921 agatactctc agaaaaatga cttttgtgac aaaggtgaag aaggcaagga ggaggtatgg 
     1981 tgggaaagag gaagacattt agaaactcag gcttcttagg taactctctt acttcggaaa 
     2041 tggaagtgtt aaggtgagat gggcccattt attactgatg acttacttgg tactttaggg 
     2101 aatgcagcaa ggtgatggca ggggtgggca ggggtttgca gagtggagac agcatgggct 
     2161 ggtgagcaca gggagaggtg gaggaagctg ggcgctggca ggcagggaaa gttcgggagc 
     2221 ctggagggag gtagactatc gggttatagg gtggggtgaa gtggacctta cagtcaggca 
     2281 ggtaagctca cgggtttcag accacaaaac gctaagatct gggcctggga agggccaagc 
     2341 cagcatgtga gaaccaagaa agcataaacc tcagagggcc aggcgccatc ccccagctct 
     2401 caggttccca ggaggatggg gaggaacaaa ggtcttgggt tttgacacat cccaaagact 
     2461 ttgggagctg cccctggtct ccctcccagg tgggaaccag gcttttccat gagcaaaaga 
     2521 agatgggaca gcatgggctg gaaagtgtgg caggggaaca cagagctgaa ttctgagttt 
     2581 tcctgaaaaa gagatataaa aacccacatg ctaacagagg gacttgagca gagaaaggga 
     2641 ctctccacag ccatactcgg gaactgtata cacaagggac tgggaagtga agcagcagaa 
     2701 aaatccagag cctgtgatga catcacagct aagggcaaag acaggaagtg gaaaatgtga 
     2761 atggggaggg aggaatggat tgagcaaggg gtacttatca gtataatcag atgctgtttt 
     2821 gcccaggttt gcaccctgtg cgcctgtcct caggacccag caggctcaga cttgcctgcg 
     2881 atcaaagaaa gccctgtgtg cctcccagac ccaccacccc cgtgcaaact ctctcaacac 
     2941 gacaccgctg cgtgactttc ctgctgttac tagaggtcgg caggtacttt tccctaggaa 
     3001 actgggtgtg cacgtctcat ccccgtgcca attctcttgc cgcttttcct tccacacgcg 
     3061 gcgtcctctg ggcggagggt gcagctccgc gtgctccagc tgccgcgaaa gttgcacccg 
     3121 gcccagtctg gctgcgggcc ccgcctccgc cttctcagcg cccagcccca ccctccgatt 
     3181 ggtcagcagt gcccagcccc gccccgctcc tccgagggga tttaaagcag cggccgctca 
     3241 gtctgggcgc ttgcaggctg ctaaacccaa ccgcagttga ctagcacctg ctaccgcgcc 
     3301 tttgcttcct ggcgcacgcg gagcctcctg gagcctgcca ccatcctgcc tactacgtgc 
     3361 tgccctgcgg gcgcgccgaa gttcctattc ttcaaaaggt ataggaactt cgccgccacc 
     3421 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 
     3481 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 
     3541 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 
     3601 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag 
     3661 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 
     3721 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 
     3781 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 
     3841 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac 
     3901 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc 
     3961 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 
     4021 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc 
     4081 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtta 
     4141 tccaatttac tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat 
     4201 gaggttcgca agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc 
     4261 tggaaaatgc ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg 
     4321 aaatggtttc ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc 
     4381 ggtctggcag taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg 
     4441 tccgggctgc cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga 
     4501 aaagaaaacg ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat 
     4561 ttcgaccagg ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg 
     4621 gcatttctgg ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg 
     4681 gttaaagata tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa 
     4741 acgctggtta gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc 
     4801 gagcgatgga tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg 
     4861 gtcagaaaaa atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg 
     4921 gaagggattt ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag 
     4981 agatacctgg cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc 
     5041 gctggagttt caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgtc 
     5101 atgaactata tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat 
     5161 ggcgatgatc caaaaaagaa gagaaaggta gatccaaaaa agaagagaaa ggtagatcca 
     5221 aaaaagaaga gaaaggtaat aagaagttcc tattctctag aaagtatagg aacttcagcg 
     5281 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa 
     5341 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa 
     5401 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 
     5461 taaagcattt ttttcactgc gaattctcgt aaaactcttc atggcagtag ttattattct 
     5521 ctctctctct ctttttcttt tttcttgaga caggatattt ctctgttgcc caggctggag 
     5581 tgcagtggca cagtcttggc tcactgcagc ctggacctcc tgggctcaag ccatcctccc 
     5641 acctcagcct cccaagtagc tggggctaca ggcacatggc caccaggccc agataatttt 
     5701 tcatttttgt agagactgag tctcaccatg ttacccaggt ttattattct cattttttag 
     5761 atgaagagac tgaggtccag agaagctcaa tgacttgcct agttttacaa atctcctgcc 
     5821 atcacatacc cctcagcgtc cttaataaga gggaggccac caactatgtg ctgggcactg 
     5881 tggtggatgc tggagctata gggttgagta tataagaaat ggtgttgctg gagcaactgt 
     5941 tgcttgctta cctgacctat ctgagaatta attagcaggg gaacatattt ttgttttcag 
     6001 attcaatata agaacttgtg tgggcaaaaa taaagatcag tagtaataac agtagttccc 
     6061 atttgctgac tgtactgtcc taagtgcata tatatataca tacacacacg catacctata 
     6121 ctcctctaat actcaaaatg atcctgttta tgtattgtta atatgctcat tttattttta 
     6181 aatttttatt tatttttatt tttatttatt tttgagacgg agtctcattc tgtcgcggag 
     6241 gctgaagtgc agtggtgcga tctcagctca gtgcgacctc cgcctcccgg gttcaagtga 
     6301 ttctcctgcc tcagcctccg gactagctgg gattacaggc gcccgcctcc acgcccagct 
     6361 aatttttgta tttttagtag agatggggtt tcgccatgtt ggccaggctg gtctcgtact 
     6421 cctgaccttg agtgatccac ctgcctcggc ctcccaaagt gctgggatta caggcatgag 
     6481 ccaccgcgcc gggctaatat gctcatttta gtgaggcaaa aatagaggct cagagtctga 
     6541 tttgtacaaa actacagagc agttaagtgt cctctcagat gtgtaccctg atctgggtga 
     6601 ctctaggact ctaggtctca actgttacaa ccagttaagg gtttggggaa gcactgggcc 
     6661 aagagtcagg aaaatggaag ccacaggtag tgcaaggtct tgggaatggg acgtctggtc 
     6721 caaggattca cgcgatgact ggaacccgaa gagccggggc ccggtttacg gccgccatga 
     6781 agcaacgcgc gccggtaggt ttgggaatca gggagccctc tgaataggag actgagttgg 
     6841 gagggaaagg ggcttcgctg ggggagcctc ggcttcttct gggagaaaat tcccacggct 
     6901 acctagtgag cctgcaaact ggtaggcgcc ggcgtaggcg cgcgggcggg gccgggggcg 
     6961 gggcctgcgg ggcgtggcgg ggcgggcaga gggcggggcc tgcttctcct cagcttcagg 
     7021 cggctgcgac gagccctcag gcgaacctct cggctttccc gcgcggcgcc gcctcttgct 
     7081 gcgcctccgc ctcctcctct gctccgccac cggcttcctc ctcctgagca gtcagcccgc 
     7141 gcgccggccg gctccgttat ggcgacccgc agccctggcg tcgtggtgag cagctcggcc 
     7201 tgccggccct ggccggttca ggcccacgcg gcaggtggcg gccgggccct gaggcgcggg 
     7261 atcgatccat gacacccccc cgccccccat atctcactat aattcactgt caaatggctt 
     7321 ttatgatttg gagtttgggg atacttttaa agacagggtc ttggtagctc ctagatggct 
     7381 tgggattcac ttagtaaccc ttgctggcct aaaattcagg aacttctacc tcatcttccc 
     7441 aagtactggg ttagtaggga tgtccagcat atttagcttg ctttaatgat tttgctgtac 
     7501 ttttttctgt gtgtacaggt gcatgtatat ttataaaact aacataatat attttacccc 
     7561 caaagtacca taaccctaat taatgctatt aaatgttttg tatgctgggc tctgggcata 
     7621 ctaatataac caatattata caatgcaaaa caattttgga atatcttctg taaaattatc 
     7681 ttattagtaa gttgtaacca tctcagactt ctaagtttga gtttgtaaag gatcagtttt 
     7741 ttttggtgac ctacacacac acacacacac acacacacac acacacacac aggggggtgg 
     7801 attctgagaa tttagagaat ttagatggtt aataccagtt tttattttta ttctgtgtga 
     7861 tatttattta ttaagattta tttatttatt acatgtaggt acactgtatc tgtcttcaga 
     7921 cacaccagaa gagggcatca gatctcgtta cagatggttg tgagtcacca tgtggttgct 
     7981 gggatttgaa ctcaggacct ctggaagaac agtcagtgct cttaaccgtt gagccatctc 
     8041 accagccccg tgatatttta tttaacttac tagaatatac tgtgttaaaa catattttag 
     8101 gggattgggg agatggccca ttctgtaaag tgtctgccat gtcatcttca ggacctgcgt 
     8161 ttgtaacccc agcatggaga agacagagac agggagaccc ctgggcttcc tggcaagcta 
     8221 gactagcaaa tttggttaac taggttcaat gagagactca gtctcaaaac ataagaagtg 
     8281 aattttttag acccaatttc aatctctgaa ctccatacgt atgcacatgt acctgcatat 
     8341 aaatatatat acacacccat aaacacatgc acacacaaaa aatggttcaa aagttcttaa 
     8401 aatgtataag agtagaaagt gtagagccgg gagtggtggc gcacgccttt aatcccagca 
     8461 cttgggagca gaggcaggtg gatttctgag ttcgaggcca gcctggtcta cagagtgagt 
     8521 tccaggacag ccagagctat acagagaaat cctgcctcaa aaaaccaaaa ataaagtgta 
     8581 tatactaatc tttcatgcct aaaatattta tttttgccac atttttcata cctctttaat 
     8641 atttttcttt ttctctttgt gcttcaaagg aaattttgtg tcatgtttta cttttcattc 
     8701 ttgactctat tcagcagtaa gacgcagcat ggctttctta gagaagtata atctattaca 
     8761 aaacggtaac atttaactgc tctacatacc ctcttgtagc taaattcctc tgttaaacta 
     8821 agacttatta tctaaacgta attaaatgtt gatatcttat aatgctccac tttgaaacag 
     8881 ctgtctttct tttgcagatt agcgatgatg aaccaggtta tgacctagat ttgttttgta 
     8941 tacctaatca ttatgccgag gatttggaaa aagtgtttat tcctcatgga ctgattatgg 
     9001 acaggttagt aagacctcga ttgaagttat ttttagaggg tgtgtgtgtg gtgtgtgtgt 
     9061 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt aagagagaga ggagaggcat gtgtgatata 
     9121 tatgtgaagt gtattgcatg tacatggaat ccagagaagg acattaggtg tcctactctg 
     9181 ttgctctctt attcccttga atcagtgtct cttgctgaac ctggggcata catactgatt 
     9241 tggctaggct gcctggctag caagacccag ggatctgctt gtctctgccc cccagtgctg 
     9301 acgttacaag caggtgcaac cacatctggt ttttatatgg gtactggggg atctgaactt 
     9361 agggcctgat gcgtgcccaa gtgctctcac ccgccaagcc acctctccag ccaaagggtt 
     9421 ttttttcttg tattaattct aacttagcaa ctgttgagta gttgatgttt gaatataagc 
     9481 taagccaact tattagaata aatgttttct attttttact tgtatgtgtg agtgcctgca 
     9541 tgtatgtatg tgaagcatat gtattccagg atgtcagaat tgtgatggat tccctggaac 
     9601 tggagttaca gaccattgtg aaccactgtg tatgggtgct gggaactgaa ctttaggtcc 
     9661 tctgcaaaaa cagtaaggat cttaactgat aaactatctt tctagcccta aggctttcta 
     9721 cttttaaagt tcgtttctct ttctctctct ctctctctct ctctctctct ctctccctct 
     9781 ccctccctcc tcctcctcct cctctttccc ccctctcgcc tcctctttct ctctcccctc 
     9841 cctctctctc tctcacacac acacacagac agagagagga gagagaaagg aagccttact 
     9901 aattaattac ctagttttag acataactag tgggaatcca tagtaagtaa atcaacattc 
     9961 ttgaagagag gttagaagaa ttggctctcg ttgtccatcg aggctgtata agattattct 
    10021 tctgttttct cagcttacca tacatatttt ctacgtagac aataccaatt caaatacact 
    10081 gtacgtaaag tatatgcgca cacacacaca cacacacaca cacacacaca cacaagttac 
    10141 atattgtcat atagtactaa aatttatgga ctttttcata ttaatacatc tagagctatc 
    10201 atgtttttta tcttttaaaa attactttac ggggctacag agatgtctca gcagttagga 
    10261 gcactgactg ctcttccaga ggtcctgagt tcaattccca gcaaccacat ggtggctcac 
    10321 aaccatctgt aatgggatct gatccactct tatggtgtgt ctgaagacag tgacagtgta 
    10381 ctcatataca tgaaataaat ctttaaaaaa ttactttacc atcagggtat atatatatat 
    10441 atatatgtgt gtgtgtgtgt gtgtgtgtat gaagtatttg ctatttgaga gggtctcact 
    10501 gtttagctca gtctggcttg gaacgttctg tatagcccag actggcctca actcagcagt 
    10561 ccccctgaaa ggctctcata tgtggagatt acaggtatgt gctactgtgc ccagccctac 
    10621 atgttacttt gtttttgaaa tagggtatca aatagcttag actggcttct aagtcagtgt 
    10681 gtagctaagg atggtctgag cttctgaacc tgctacttca actcctggtg tgccatcatg 
    10741 gccagtttgc acagttgtga gagtagaact caggacctcc tatgtgttac tagacatgtt 
    10801 ctctaccagc tgagctacat cccaattcgc ctacatttac ttttattttt taaaagattt 
    10861 atttatgtat acagcactct gcctgcatgt acatctgcat gccagaagag ggcaccagat 
    10921 ctcatcatac atagttgtga gccaccatgt ggttgctggg gattgaactc agggcctctg 
    10981 gaagagcagt cagtgctctt aacctctgag ccatctctgt agccccctac atttatttta 
    11041 aagattatag gctattctat tcatagatgt gccataactg atttaaccag atcttcatta 
    11101 ttttgtactt agtagagtat attagaggga atgaatctta aatacacatt ttgacacttg 
    11161 ctcagcttta gacaagtaaa aatcactggt caaggaaatg gtgcttagtg gatgcttctg 
    11221 aactgtcaaa ttgtcctcca acaagttaca tcattttata cttccaacgt tatgtgagag 
    11281 ttcatttttc tttaggctct tctagtattt tatatttgag aaataaaatt taaccaaaat 
    11341 ttgccaatct agtaagcttg tttttatttg tatctttaaa ttttatgata gatgttatag 
    11401 tgtactctcc tctcccctcc cctcccctct cctcctcagc aggatcttgt tatgtagccc 
    11461 taagggatct aatacttact ctgtagtcca ggctgtcctt cagctcatga gacacaccat 
    11521 catgcttggc atgtttcttg ttctgaaagg tatctggttt taattttggt tgagctggtg 
    11581 tgttggtgta tatctgtaat ctcagcacag gcaagtagag acaaggatta tgattacagt 
    11641 ccagcctggg ctatttagag ggattctctt taatctttcc tcatgcccca aaatcttacc 
    11701 tttggtatat gaaaaatagt ctccacttct gcaaaatatt gctttatgaa gtaagaatta 
    11761 attcccttca tagagacaag gaatgtgtcc tgtaaaagtt taatgtgtaa gaagtatttg 
    11821 ttataaaaga taaatattca gaatcttctt tttaattcct gattttattt ctataggact 
    11881 gaaagacttg ctcgagatgt catgaaggag atgggaggcc atcacattgt ggccctctgt 
    11941 gtgctcaagg ggggctataa gttctttgct gacctgctgg attacattaa agcactgaat 
    12001 agaaatagtg atagatccat tcctatgact gtagatttta tcagactgaa gagctactgt 
    12061 gtaagtataa ttaacttata attaaaaaaa tagggccatt ctagttttat ctatattttt 
    12121 tttaaacttg tgcaaactat gctacataat caacttactt gattatgtaa tatatatagt 
    12181 aatagttgga attactatta ttacatattt atttttctgt gctggggtag aacccaggga 
    12241 actaagtact gtaataaccg agcttcattc atcctagtca tttttaattt tttttttttt 
    12301 ttttgttttt tcgagacaag gtttctctgt gtagccctgg ctgtcctgga actcactctg 
    12361 tagaccaggc tggcctcgaa ctcagaaatc tgcctgcctc tgcctcccag tgctgagatt 
    12421 aatgacgtgc accaccactg cctggcattt ttttaaagtt ttaatttgag accctttctc 
    12481 actaacttgc tcacactatc ctcgaactca ttacgtgggc tggtctagaa atttcagtca 
    12541 acttgcctca gcttctcaag tagctgggat tacaggcagg tgctaccact tctggccatg 
    12601 atcattgttg ttgttgctat tattattatt attatttcac tgatacttca tatcacaaca 
    12661 cagaatagta actgtagttc atgaccaaca aaaaagttgt acagtttctc tgttgtctta 
    12721 ttttgatctg gattttgatt tgtttcatgg cactgggcag gctaatctca aactgcatgg 
    12781 ctcaaatagt ccttctgtct taatctccca tgttctggaa gtataggtat cactacacct 
    12841 agctgtattt tcatatttta aaaaaaacgc ctaagaattg ctattgaatg aggcggagag 
    12901 atggctcagc agtcacaagt acttgtagag gactggggtt tggttactag cacctcaaag 
    12961 atggcttaca attatctgta gctccaattc cagaggatct gtgtggtaaa gctgcatgta 
    13021 tatgggcatg caggtaaaac tgctaaacat ataaaaatag ctcagtctta agagaattgg 
    13081 tcgactgtcg actttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 
    13141 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga 
    13201 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 
    13261 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg 
    13321 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata 
    13381 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg 
    13441 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac 
    13501 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact 
    13561 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat 
    13621 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga 
    13681 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac 
    13741 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat 
    13801 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac 
    13861 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct 
    13921 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac 
    13981 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga 
    14041 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg 
    14101 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact 
    14161 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac 
    14221 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta 
    14281 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt 
    14341 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga 
    14401 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc 
    14461 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac caggtcgagt 
    14521 agggataaca gggtaatgga tcctcgttgg gcacctgcac tcatgtacgc aggcccacat 
    14581 atagatgtac acacgcatat cattcaaaat agaaattatt tatacaagta gtgcgttgga 
    14641 gagctggctc aggagttgtt aatgcttgct acccttgcaa aggacccaag ttcaatttcc 
    14701 agcacctgtg ttgggcatct cacaaccaca tgtaaatcta gtttcagggg atttcacccc 
    14761 tcttctgaac tcttcaggca cttataccca caaatggaca tctccacata gagacacata 
    14821 cttttaaaaa agtaatctag ggtctagaga aatggttata gaccacggac tgggatcagt 
    14881 tcccagaagc cacatcctgt ggctcacaat tgcctgtgaa tcaagttcta gatctggtgg 
    14941 cctcgtctag cctgcagtca tcaacaaaca catgcacaca tccatacaca cataatttaa 
    15001 aagaaagcga aagtaaccta taatgctaaa caaagcacat tctcagtgta gactgcagtg 
    15061 gtgatgaacc ttgaaaatat attaatagaa gccagacacg taagtcacaa atggtataac 
    15121 tcaatttata tgaaattccc agagagtcca aagttaaaaa aaacaaaaag gcagattatt 
    15181 ggttgtcagt agctacagag atgggacatt gaagatatta ataggtatag ggttgaggat 
    15241 tcatggcctt tccctcttcc atgttagcat gtctattgtt gttaacctta ttcaactcat 
    15301 gttagggcaa tcatattgat gagacttaat gagtatagct tgacattcct agaagacaga 
    15361 gtctcacatc agactctctg atcctctgac tcttagaatc tttcaacacc ctcttctgaa 
    15421 atgatctctg agcctttggt tgtgactgaa ttatgagaaa gaattttatg ttgaatgaat 
    15481 tttacttcag catttttaca aaactgaagc tggggatggc gtttcatgcg tgcagtccct 
    15541 accatttgcc tataagcctg gaggaggcag aagtagaatt aggcttttca gggacatctt 
    15601 cagctgcatc atgagcttga ggacaacttg agctacataa gattcccatc cccaccccca 
    15661 cacacacaca cacatctagt atgtacctag tatgcagtct tttcatatgt ttaaaatgta 
    15721 gaaaggtata tgtgaagtct cccttttccc cacaacttag tccctccaaa gtattgcatc 
    15781 agtttccttg tacatgattt tatgcatata taagatacac tatttctctc tctctctctc 
    15841 tccttccctc cttccctcca acctctgggt atgagttgag ggttatgtat gtttcaagtt 
    15901 gtaataagaa ttgccactca aacatttcat cccattttaa gttaagtttg tctgattttc 
    15961 cactttagaa gggcagccat gatggctcat gcctataatc ccatcctagc acttgtaagc 
    16021 ctaatacata agactggagg attgtcatca gcttgaggct agcctggtct acctaatgag 
    16081 ttccagccag ccaagagtag aatataagac cctgtctcca aaaaatgtac caaaggggct 
    16141 ggaggggtaa ctcagtggtt aagaacagta gctgctcttc cagaggacct gggttcaatt 
    16201 cccagcaccc acatggcagc ttgcaactat ctgtaactgt ccagctttgg gatatccgac 
    16261 accattatac caattacata aaatgaaaaa attgtaaaat gtgctaaaat gagtggggag 
    16321 ggggagcatg gtgtggttct gtgggcttgg gattttgcca tgaatccatg actatatctc 
    16381 agcaatatgt ggattatttc tggaaacctg tggggcatgg gtgtatggga agaggggaga 
    16441 gagagagagc ccgcatccag ccagagttcc tgtgctctgg gcaggcggac gtgggaggac 
    16501 tgctggacga tttccacttg gccctgggtg ggcatctggc tgtgtgaagc cactgacccc 
    16561 actcagcggg gggtagataa ggggcatttc ccataccagg ttctcagact ccagcattcc 
    16621 acgctggata cagcagaaga gctgagaaca gaataggggc cccagggaga cactctcgtc 
    16681 ccagagataa agaaggcaga gggagagaga ttcctacgca ggcgagaggc cttagtctgg 
    16741 gccttgatga acagagacgg tctatggttt tagagcttta ttgtagaaag gcagggggaa 
    16801 agagagaagg tagaaagaga gagaccagcc atggccaaga ggagagaagg ggggaaagga 
    16861 gagagagaag aaaggctaga gagtaagaaa ggttaaagag acagaagtaa aagcttagag 
    16921 taagagagag agagagagca agagggagag taagaaaagt aaaagcttaa gagagcgagg 
    16981 aggggccagc ccctctaata gtgggcttgt tatcttgttg ttgctaggta actgtgaagc 
    17041 gtctagcctg aaggccagaa gcttgggaca ttgtctatgt gactgaaagc catgagcctc 
    17101 tactgtgggg gctgtggagg tggtaacttg gacaggagcc aggggttcca ggagacatga 
    17161 gggaatgcct tctgtcccat gtaggtagga attaccacca ccggctttca ccgtagttca 
    17221 ctggagactg tttgtacata gcccattgcc ccacagaaac caggcactca ctgtttcatt 
    17281 tgcataagaa ttagaaggct ttattttggc tgggattggt gacttgcccc agaaattcca 
    17341 gcttttggga agccgaatcc tggagaacca ggagttcaag gtcatcctca ggtacctcat 
    17401 ttgttcaaga ccagcctgtg ctaccatgag accttgtccc tactcccctc aaccccaaaa 
    17461 cacgctgttt tcattttata ggttgtacta accatggagg atggtcattt gcctttctct 
    17521 tgatttcaga agcgtagcgc gggctttgca ccatgatcta gtgtttccag ataggcttgc 
    17581 ctctccacag ccaggcttat cctgcttcag tcccatgttt ggcaagggca acatatgact 
    17641 gaagaacttg catagcctta tggtgtatca tgactcttag ccccagttta taactgtcta 
    17701 acagctggtg gcttttgtga aaaaggaaag catcatttga acttttaact tttcagctgt 
    17761 ttgttttgac tttgcataaa tactgagact agctaggaaa tgagcttttg tttcatttgc 
    17821 taaaaatgat ttttttttca tgaggcaatt tcaagcaatt tgaatttagc aatctctact 
    17881 tgtctgaaaa gaagttatta attaaaaaaa aacaaacaaa caggaaccta ttatgctggc 
    17941 tagtcacatg aaagaatcca aatgaacaca gtcaaggctg aatcaacaca tgaaattatt 
    18001 ttctaattcc ccaaattaca tttatgctaa ttagtatgta gtaaaacatt ttaattgttt 
    18061 ccaatgttaa acattacaga aataattttg atgttttcct aataacccag cctttgatca 
    18121 tgtttgcatt tatttgattg ttgtcagttt attaaataat taagtaagag caacaagata 
    18181 aaatgacttc atgagagtcc ttttctgtaa cttagaacgt cagtagtcat aggaactgcg 
    18241 gtcgttccct tacctttcag aagtaggcta aagagttgaa cgcaaaggtg gtttttcgaa 
    18301 tcttcctgat ccttaagccc ccactaggtt tatattctat atttgatatc ggattcgaac 
    18361 ccctcgagct taggatccgg aacccttaat ataactaagt tattaggtcc ctcgaagagg 
    18421 ttcactagta ctggccattg cggccgcttc gaggctgcct cgcgcgtttc ggtgatgacg 
    18481 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 
    18541 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag 
    18601 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga 
    18661 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 
    18721 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 
    18781 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 
    18841 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 
    18901 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 
    18961 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 
    19021 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 
    19081 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 
    19141 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 
    19201 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 
    19261 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 
    19321 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 
    19381 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 
    19441 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 
    19501 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 
    19561 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 
    19621 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 
    19681 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 
    19741 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 
    19801 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 
    19861 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 
    19921 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 
    19981 cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 
    20041 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 
    20101 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 
    20161 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 
    20221 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 
    20281 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct 
    20341 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 
    20401 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 
    20461 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 
    20521 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 
    20581 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 
    20641 tccgcgcaca tttccccgaa aagtgccacc tgacgg 
//
