LOCUS pCru5-CCC/ACC-mE 6983 bp DNA circular 17-OCT-2013 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|676156644| COMMENT VNTDBDATE|676156644| COMMENT LSOWNER| COMMENT VNTNAME|pCru5-CCC/ACC-mEGFP-IRES-mCherry| COMMENT VNTAUTHORNAME|Josh| COMMENT VNTOAUTHORNAME|Demo User| FEATURES Location/Qualifiers CDS 1393..2103 /vntifkey="4" /label=mCherry LTR 5615..5691 /vntifkey="19" /label=U5 /note="U5 region of MoMLV LTR" promoter 4948..5515 /vntifkey="29" /label=P\CMV /note="Human cytomegalovirus (CMV) immediate early promoter" misc_signal 5761..6595 /vntifkey="87" /label=Psi /note="Psi packaging signal" LTR 2676..2752 /vntifkey="19" /label=U5 /note="U5 region of MoMLV LTR" LTR 2159..2607 /vntifkey="19" /label=U3 /note="U3 region of MoMLV LTR" LTR 2608..2675 /vntifkey="19" /label=R /note="Repeat region" LTR 5547..5614 /vntifkey="19" /label=R /note="R region of MoMLV LTR" enhancer 2259..2432 /vntifkey="9" /label=MoMLV\Enhancer /note="ColE1 origin of replication" rep_origin 3092..3735 /vntifkey="33" /label=ColE1\Ori /note="ColE1 origin of replication" CDS complement(3889..4746) /vntifkey="4" /label=AmpR /note="Ampicillin resistance gene (beta-lactamase)" RBS 790..1365 /vntifkey="32" /label=IRES /note="Internal Ribosome Entry Site (IRES)" splicing_signal 5749..5753 /vntifkey="38" /label=Splice\donor /note="Splice donor" splicing_signal 6682..6692 /vntifkey="38" /label=Splice\acceptor /note="splice acceptro" CDS 34..762 /vntifkey="4" /label=mEGFP misc_feature 17..39 /vntifkey="21" /label=TIS:\\CCC/ACC BASE COUNT 1646 a 1986 c 1777 g 1574 t ORIGIN 1 tcgagtaact aactaaccca tgggttgatt accatgggcg aattcagcaa gggcgaggag 61 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag 121 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc 181 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac 241 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc 301 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac 361 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag 421 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac 481 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag 541 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc 601 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag 661 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc 721 gccgggatca ctctcggcat ggacgagctg tacaagttcg aataagcatg catctagggc 781 ggccaattcc gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa 841 taaggccggt gtgcgtttgt ctatatgtga ttttccacca tattgccgtc ttttggcaat 901 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct 961 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct 1021 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc 1081 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa 1141 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc 1201 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg 1261 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc 1321 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataagcttg ccacaacccg 1381 ggatccgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc 1441 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc 1501 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt 1561 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc 1621 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc 1681 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc 1741 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc 1801 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac 1861 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc 1921 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc 1981 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg 2041 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag 2101 taagcggccg ctcgacgata aaataaaaga ttttatttag tctccagaaa aaggggggaa 2161 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat 2221 ggaaaaatac ataactgaga atagagaagt tcagatcaag gtcaggaaca gatggaacag 2281 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa 2341 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc 2401 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag 2461 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg 2521 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat 2581 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg 2641 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct 2701 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttccgactt 2761 gtggtctcgc tgccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc 2821 ttcacatgca gcatgtatca aaattaattt ggtttttttt cttaagtatt tacattaaat 2881 ggccatagtt gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattggcg 2941 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 3001 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 3061 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 3121 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 3181 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 3241 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 3301 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 3361 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 3421 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 3481 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 3541 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 3601 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 3661 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 3721 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 3781 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 3841 gcgcaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 3901 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 3961 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 4021 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 4081 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 4141 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 4201 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 4261 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 4321 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 4381 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 4441 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 4501 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 4561 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 4621 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 4681 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 4741 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 4801 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 4861 ccgaaaagtg ccaccagctt tgctcttagg agtttcctaa tacatcccaa actcaaatat 4921 ataaagcatt tgacttgttc tatgccctag ttattaatag taatcaatta cggggtcatt 4981 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg 5041 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac 5101 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt 5161 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa 5221 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta 5281 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg 5341 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg 5401 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc 5461 attgacgcaa atgggcggta ggcatgtacg gtgggaggtc tatataagca gagctcaata 5521 aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt 5581 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt 5641 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat ttgggggctc 5701 gtccgggatc gggagacccc tgcccaggga ccaccgaccc accaccggga ggtaagctgg 5761 ccagcaactt atctgtgtct gtccgattgt ctagtgtcta tgactgattt tatgcgcctg 5821 cgtcggtact agttagctaa ctagctctgt atctggcgga cccgtggtgg aactgacgag 5881 ttcggaacac ccggccgcaa ccctgggaga cgtcccaggg acttcggggg ccgtttttgt 5941 ggcccgacct gagtccaaaa atcccgatcg ttttggactc tttggtgcac cccccttaga 6001 ggagggatat gtggttctgg taggagacga gaacctaaaa cagttcccgc ctccgtctga 6061 atttttgctt tcggtttggg accgaagccg cgccgcgcgt cttgtctgct gcagcatcgt 6121 tctgtgttgt ctctgtctga ctgtgtttct gtatttgtct gaaaatatcg gcccgggcca 6181 gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg agcggatcgc 6241 tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct ctgcagaatg 6301 gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc tcatcaccca 6361 ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg tcccctacat 6421 cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct ttgtacaccc 6481 taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac ctcctcgttc 6541 gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg cccccatatg 6601 gccatatgag atcttatatg gggcaccccc gccccttgta aacttccctg accctgacat 6661 gacaagagtt actaacagcc cctctctcca agctcactta caggctctct acttagtcca 6721 gcacgaagtc tggagacctc tggcggcagc ctaccaagaa caactggacc gaccggtggt 6781 acctcaccct taccgagtcg gcgacacagt gtgggtccgc cgacaccaga ctaagaacct 6841 agaacctcgc tggaaaggac cttacacagt cctgctgacc acccccaccg ccctcaaagt 6901 agacggcatc gcagcttgga tacacgccgc ccacgtgaag gctgccgacc ccgggggtgg 6961 accatcctct agactgccgg atc //