LOCUS       Ple2\(pEMS1142)        20441 bp    DNA     circular     15-FEB-2010
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|546013317|
COMMENT     VNTDBDATE|546013317|
COMMENT     LSOWNER|
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COMMENT          #141=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 
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COMMENT          #142=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 
COMMENT                1 1 "@N" 0) 
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COMMENT         (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 
COMMENT          (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 -365) 
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COMMENT        (mapper: 23.2135 -15.4958 40.0378 40.0378 0.01 10 -1 20441 20441 1 0 0) 
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COMMENT                            1.74233 0.833333 0 "Ple2 (pEMS1142)" "@N" 1 0 
COMMENT                            0.871165 0 -10 9.99056 1.47361 Nil) 
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COMMENT               #150=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -1092 100) 
COMMENT                     (CObjectList 
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COMMENT                                   7.99245 1.17389 #152#) 
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COMMENT                                   14.7265 2.57257 0 -10 2.67247 1.17389 #154#) 
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COMMENT                                   "Tetracysteine residue" "@N" 1 18.7429 
COMMENT                                   -0.368628 0 -10 10.0405 1.17389 #156#) 
COMMENT                             #158=(CScratch 
COMMENT                                   (CWidget 0 (3 #8# 0) 1 2 0 0 #114# 828 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 4.76266 1.9 
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COMMENT                                   16.9016 -2.88523 0 -10 1.99811 1.17389 #158#) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 early polyA signal" "@N" 1 17.8148 
COMMENT                                   -6.46979 0 -10 11.0645 1.17389 #160#) 
COMMENT                             #162=(CScratch 
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COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.01155 1.9 
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COMMENT                                   "FFRT (F5 mutant)" "@N" 1 16.3767 5.88239 0 
COMMENT                                   -10 7.99245 1.17389 #162#) 
COMMENT                             #164=(CScratch 
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COMMENT                                   15.8012 -4.92727 0 -10 2.52262 1.17389 #164#) 
COMMENT                             #166=(CScratch 
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COMMENT                                    100) (LOGPEN 0 8 13395507) 10 1 1.88954 1.9 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "I-Sce-I" "@N" 1 
COMMENT                                   -13.4048 -4.50869 0 -10 3.02214 1.17389 #166#) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                   "Ple2 (ADORA2A-B)" "@N" 1 -0.27454 4.91524 0 
COMMENT                                   -10 8.51695 1.17389 #168#) 
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COMMENT                                   "@N" 1 14.1305 7.42491 0 -10 6.16917 1.17389 
COMMENT                                   #170#) 
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COMMENT                                   1 12.7211 10.1163 0 -10 4.19603 1.17389 #172#) 
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COMMENT                             #175=(CLabel (CWidget 0 (0 0) 1 2 0 0 #106# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                   1 8.24545 13.1514 0 -10 4.19603 1.17389 #174#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #176=(CGroupWidget 
COMMENT                            (CWidget 22 (7 22 0) 1 2 0 0 Nil -883 100) 
COMMENT                            (CObjectList 
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COMMENT                                    (CWidget 0 (3 #1# 0) 1 2 0 0 #122# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 1 
COMMENT                                    0.00184135 0.376864 1 0.082322) 0.8 1.8 1) 
COMMENT                             #178=(CLabel (CWidget 0 (0 0) 1 2 0 0 #106# 8 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "AmpR" "@N" 1 
COMMENT                                   -5.43447 12.3462 0 -10 2.87229 1.17389 #177#) 
COMMENT                             #179=(CWideArrow 
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COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 0.835356 
COMMENT                                    1.89138 2.32901 1 0.082322) 0.8 1.8 0) 
COMMENT                             #180=(CLabel (CWidget 0 (0 0) 1 2 0 0 #106# 2 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                   -11.9269 -6.96932 0 -10 2.87229 1.17389 #179#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #181=(CGroupWidget 
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COMMENT                                   "5' homology sequence" "@N" 1 -16.2064 
COMMENT                                   3.48466 0 -10 10.4901 1.17389 #182#) 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "3' homology sequence" "@N" 1 -8.36512 
COMMENT                                   -12.1963 0 -10 10.4901 1.17389 #184#) 
COMMENT                             #186=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #4# 0) 1 2 0 0 #110# 0 100) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "HPRT complementary sequence" "@N" 1 16.4668 
COMMENT                                   -8.71165 0 -10 14.9609 1.17389 #186#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #188=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -325 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #189=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil 1237 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #190=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -395 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 
COMMENT        (CObList #191=(AnlzFontBackColorItem 3336 4102 33554687) 
COMMENT         #192=(AnlzFontBackColorItem 2674 3335 50331392) 
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COMMENT       (CStringList) 4064080 14 (CObList)))
FEATURES             Location/Qualifiers
     misc_recomb     14304..18115
                     /vntifkey="86"
                     /label=5'\homology\sequence
     misc_marker     complement(19214..20435)
                     /vntifkey="22"
                     /label=AmpR
     misc_marker     12853..14278
                     /vntifkey="22"
                     /label=AmpR
     misc_recomb     9212..12857
                     /vntifkey="86"
                     /label=3'\homology\sequence
                     /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3"
     misc_recomb     5258..9211
                     /vntifkey="86"
                     /label=HPRT\complementary\sequence
                     /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2"
     misc_feature    4160..4170
                     /vntifkey="21"
                     /label=Kozak\consensus
     misc_feature    4167..4886
                     /vntifkey="21"
                     /label=EGFP
     misc_feature    4887..4922
                     /vntifkey="21"
                     /label=Tetracysteine\residue
     misc_feature    4923..4995
                     /vntifkey="21"
                     /label=NLS
     misc_feature    5202..5252
                     /vntifkey="21"
                     /label=SV40\early\polyA\signal
     misc_feature    4112..4159
                     /vntifkey="21"
                     /label=FFRT\(F5\mutant)
     misc_feature    5001..5048
                     /vntifkey="21"
                     /label=FFRT
     misc_feature    14285..14302
                     /vntifkey="21"
                     /label=I-Sce-I
     misc_feature    153..4103
                     /vntifkey="21"
                     /label=Ple2\(ADORA2A-B)
                     /note="1307"
     misc_feature    3337..4103
                     /vntifkey="21"
                     /label=Prom\Region
     misc_feature    2675..3336
                     /vntifkey="21"
                     /label=Region\4
     misc_feature    153..2674
                     /vntifkey="21"
                     /label=Region\3
BASE COUNT     5281 a      4725 c      4799 g      5636 t 
ORIGIN
        1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 
       61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 
      121 cgagctccta ggccggccac gcgtggcgcg ccaaacttcc cggatgatgc tggggtggag 
      181 tggggagcca ggggaaacca taggacctca gagttaggag ccgcccgcca cctgccttcc 
      241 cccagatcct ctgggccagc cttgcagtgt gcacattagg aaaatgaccc agagagggga 
      301 agtgacttgg ttgaatgcca cagtgagtca cttggaccta tatgcaaatt ctgttgctcc 
      361 tcaagtcacc aaggactcat cgtcttagag gcagccagtg tgctcagggg ctggctgttg 
      421 gaggggattt ttatggtgaa tcttttgtga agtgacttca gccaagtaat tgtgcaaatg 
      481 aagattttcc cagaggctga attactcggc atggattcca cctacccagg ccatagatga 
      541 ggctagaaag tgcatccctc ccttggggac gtcagaggcc ttctgcccca cctcctggcc 
      601 cctctgccac agagctgggt tccacagcct gggtggttgt gggccctgag gccatgtatg 
      661 aggcttgagg tggtgcctct tgagagtcag ggagcagggt gcaccatctc tgcggtgaat 
      721 tatctgaaac tggtgggtgc ctatctaaag aggaagccac agcaggttga gggtgcccca 
      781 gcaggaagga ggacccagct agatgagcaa atggaaattt agagcgtcaa agccaagggg 
      841 cccttagagg tcatttcctc tcatcttcgg gatggaaaat gagcctcaga gaagacagcc 
      901 agctgtttcc gggccacaca acgagttggt agtggagctg gggcgagtcc tggaaagaaa 
      961 tgtggcctga tccccactgt gacttcagtc actggggagc ggtcatgagc agcctgtctc 
     1021 gcagagcctg caccaggtgg ctgtccctgt cacctgggct ctaagtggga gcaggagctg 
     1081 cactgggctg ctccagggaa ggggaggaat ctggacctca cactgccctg tcagatgctc 
     1141 tctatcccag gagagtctag aaccctcggc aaaggagaag gggaccctgg ccagccctga 
     1201 gaggggtgga attagggatt gtcggactcc aagtgcagtc tctggtgagc ctccatccat 
     1261 tgctctgcga cgggcagagc agagtttagg ttcgtccagt ggcagctcag aacacggagg 
     1321 cccaggaggg gccctgtctg caggtgccca ggtgggccag agaacaaagc actggagggg 
     1381 acttcaggga accaagctga gtctgcacac tgatgggatg ctctatgggg aagcagaggc 
     1441 caagctctca cttttttctc tgattcaaag ggtatgatca gggctgccgt tctcaattta 
     1501 aaggagctcg actttttttc tcttttgtct ctccctttcc ttctctgttt tctgagagtt 
     1561 gagagaatga ctccctatgt agtaggacag gatgtgctgt gtggctatgg aaagttagct 
     1621 gcctctctct gggcccattt cctcatccat aaaataggac aagtctctct gcccctctga 
     1681 gctcacaaag gtgttctgaa ggtccagtga gtggatgggg tgacagggac cctgaggagg 
     1741 gttggaggcg tggggagggt ggggattcag atctttgggt tcagagccat ggacctgccc 
     1801 cctatcctct acccatctca tcccaacttc cttggagctg gaccttagtt ccacccagac 
     1861 cttgagccat ttgactctgc tccatgaggc tctgcaaggc ttaggctggt ctcttctgca 
     1921 gggggccctg gagaggatga cgacgatgat gttcatcctc ccttgagggc agagagcttt 
     1981 gcagttgcca aaggatgtca gctcagcatc tcctgtcatc ctcttggcct gcattgtggc 
     2041 caggcggatg ttctgaccct cctgttactc acaaactcat ggggaggcca gagcagggaa 
     2101 ggacatacga gtgtctcctg atgtggatgg actccagggc ccttcaggct gttcctggcc 
     2161 cgcaggtccc ttcgctttcc tgttcctcag ggcccctggg cctctgggca tgtggaggtg 
     2221 tccatgtggg ctcccgagag caagcggggt atgaaaggaa cttggtgaca ctcctggccg 
     2281 gcgctggagt cacagcctgc ctcaggatcc tccctcctcc tttctcagct gggaatccag 
     2341 cctcctctac gtgccgtttc ctgctccact cagaaaggtc cattcggcta gccctgggtc 
     2401 tagcctcagg cctgccccac tctccattaa cttttttttt aaaaaaaaga actcagtttt 
     2461 ggaaaaatct gttattttcc aagttcaggg aacctgttgg gctagcacag ggcctagtcg 
     2521 aggaactctg tgagaaagac ttcaggccag aggcagagat gtagctctct gtgttcccag 
     2581 aggataggga attcagggtg agagacacag gcttcttttt ccagttccca ggggattttt 
     2641 gcctcactgt cccctccctg gctctcacag cagctgaata aacttagtct atttgcaatt 
     2701 taagaaaaaa aaaagtcatg ttttgcccac tctaaaacat taaaaaggaa caaaagagta 
     2761 tagtatagta gaaagttgtc ctccataccc cctgtccccc accctcagtt ccctgcctgc 
     2821 aaggccatgt taagaatcct tccagagata ttctttgcat atataagcaa gtgctattaa 
     2881 tgtatgtttt gtttctgtcc ccacacggga ctttctttgc agagtacggt ggaagactcc 
     2941 ccttgtgggt tcccttttct gtacaagtca acaattggac agaccaggga tttccatact 
     3001 gtgttcccag aagccttcgg gttggctctg gtgtccccac caattcactg attcaataaa 
     3061 catttaataa gccccttccc ttccccagca gcttcacctc tgtgactgcc tgggaatgtg 
     3121 ggcgacaagg tttgctgctg agaacagttt gaaaaccagc aaagcaggtg gcccagggcc 
     3181 caggcagctc tgcaacaccc ctgtggctgc ttggctctgc tctgttgcct caaatgacag 
     3241 gaaactcact attgcctgag gcaggccatc tctcctgtga acagctcagc tgaaaaactc 
     3301 ttaacctacc ctgagttaca atctgcctcc ctataaggct gcccgagtcc acgtgaaagg 
     3361 cagcctctga cctcttgtcc ccaggcaggt ggtggcggct ggcaacacac tcatagggcc 
     3421 ccatgagggt tcaggattgg agggggtaga tttgggggcc tcttagctct caaagagggg 
     3481 caatttaacg ggcagaggtc catttggatc cagaccattc acaggctcga aggggacact 
     3541 gggagttggg gctgggctca ggcctgctgg ggaaccaaaa cttctgcttc tgaggggtgt 
     3601 cagtaattcc attttgactc tgggggagcc attttaaatc tgtttatttc cttctcatag 
     3661 aatcatgggt gagagctggc atggccccta gaggtcattt ggggtccagc tgcctcaccg 
     3721 tatcaatgag gaaactgagg cccagaaaag aaaagcattt ttgcccagag tccctcagtg 
     3781 agtcctggtt ccgtacctgc tttctgccag ggaccaaact cccgttagtc attcctggtg 
     3841 tcagccaggc agaggagcag gtgggggaga gggcgtggga ggagggaggt gaggcagctc 
     3901 cctccgagtg gaagctcaca gtcagctgca gcgccgcccc tgccctgact gcagctgact 
     3961 ggcaggggca cctggaggca gcgggttcgg gcgcagtatg agagggctta ccctctgcgg 
     4021 cagtgggaga caggaccagg cagctcccca catccttcca catccgagct ccaggggtct 
     4081 ctggcttgtc ctttcacagg tcgggcgcgc cgaagttcct attccgaagt tcctattctt 
     4141 caaaaggtat aggaacttcc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg 
     4201 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg 
     4261 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg 
     4321 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct 
     4381 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag 
     4441 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg 
     4501 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca 
     4561 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct 
     4621 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca 
     4681 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg 
     4741 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc 
     4801 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc 
     4861 tcggcatgga cgagctgtac aagttatttc tgaactgctg cccgggctgc tgcatggaac 
     4921 cggatccaaa aaagaagaga aaggtagatc caaaaaagaa gagaaaggta gatccaaaaa 
     4981 agaagagaaa ggtaatataa gaagttccta ttccgaagtt cctattctct agaaagtata 
     5041 ggaacttcag cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt 
     5101 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat 
     5161 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 
     5221 aaatttcaca aataaagcat ttttttcact gcgaattctc gtaaaactct tcatggcagt 
     5281 agttattatt ctctctctct ctctttttct tttttcttga gacaggatat ttctctgttg 
     5341 cccaggctgg agtgcagtgg cacagtcttg gctcactgca gcctggacct cctgggctca 
     5401 agccatcctc ccacctcagc ctcccaagta gctggggcta caggcacatg gccaccaggc 
     5461 ccagataatt tttcattttt gtagagactg agtctcacca tgttacccag gtttattatt 
     5521 ctcatttttt agatgaagag actgaggtcc agagaagctc aatgacttgc ctagttttac 
     5581 aaatctcctg ccatcacata cccctcagcg tccttaataa gagggaggcc accaactatg 
     5641 tgctgggcac tgtggtggat gctggagcta tagggttgag tatataagaa atggtgttgc 
     5701 tggagcaact gttgcttgct tacctgacct atctgagaat taattagcag gggaacatat 
     5761 ttttgttttc agattcaata taagaacttg tgtgggcaaa aataaagatc agtagtaata 
     5821 acagtagttc ccatttgctg actgtactgt cctaagtgca tatatatata catacacaca 
     5881 cgcataccta tactcctcta atactcaaaa tgatcctgtt tatgtattgt taatatgctc 
     5941 attttatttt taaattttta tttattttta tttttattta tttttgagac ggagtctcat 
     6001 tctgtcgcgg aggctgaagt gcagtggtgc gatctcagct cagtgcgacc tccgcctccc 
     6061 gggttcaagt gattctcctg cctcagcctc cggactagct gggattacag gcgcccgcct 
     6121 ccacgcccag ctaatttttg tatttttagt agagatgggg tttcgccatg ttggccaggc 
     6181 tggtctcgta ctcctgacct tgagtgatcc acctgcctcg gcctcccaaa gtgctgggat 
     6241 tacaggcatg agccaccgcg ccgggctaat atgctcattt tagtgaggca aaaatagagg 
     6301 ctcagagtct gatttgtaca aaactacaga gcagttaagt gtcctctcag atgtgtaccc 
     6361 tgatctgggt gactctagga ctctaggtct caactgttac aaccagttaa gggtttgggg 
     6421 aagcactggg ccaagagtca ggaaaatgga agccacaggt agtgcaaggt cttgggaatg 
     6481 ggacgtctgg tccaaggatt cacgcgatga ctggaacccg aagagccggg gcccggttta 
     6541 cggccgccat gaagcaacgc gcgccggtag gtttgggaat cagggagccc tctgaatagg 
     6601 agactgagtt gggagggaaa ggggcttcgc tgggggagcc tcggcttctt ctgggagaaa 
     6661 attcccacgg ctacctagtg agcctgcaaa ctggtaggcg ccggcgtagg cgcgcgggcg 
     6721 gggccggggg cggggcctgc ggggcgtggc ggggcgggca gagggcgggg cctgcttctc 
     6781 ctcagcttca ggcggctgcg acgagccctc aggcgaacct ctcggctttc ccgcgcggcg 
     6841 ccgcctcttg ctgcgcctcc gcctcctcct ctgctccgcc accggcttcc tcctcctgag 
     6901 cagtcagccc gcgcgccggc cggctccgtt atggcgaccc gcagccctgg cgtcgtggtg 
     6961 agcagctcgg cctgccggcc ctggccggtt caggcccacg cggcaggtgg cggccgggcc 
     7021 ctgaggcgcg ggatcgatcc atgacacccc cccgcccccc atatctcact ataattcact 
     7081 gtcaaatggc ttttatgatt tggagtttgg ggatactttt aaagacaggg tcttggtagc 
     7141 tcctagatgg cttgggattc acttagtaac ccttgctggc ctaaaattca ggaacttcta 
     7201 cctcatcttc ccaagtactg ggttagtagg gatgtccagc atatttagct tgctttaatg 
     7261 attttgctgt acttttttct gtgtgtacag gtgcatgtat atttataaaa ctaacataat 
     7321 atattttacc cccaaagtac cataacccta attaatgcta ttaaatgttt tgtatgctgg 
     7381 gctctgggca tactaatata accaatatta tacaatgcaa aacaattttg gaatatcttc 
     7441 tgtaaaatta tcttattagt aagttgtaac catctcagac ttctaagttt gagtttgtaa 
     7501 aggatcagtt ttttttggtg acctacacac acacacacac acacacacac acacacacac 
     7561 acaggggggt ggattctgag aatttagaga atttagatgg ttaataccag tttttatttt 
     7621 tattctgtgt gatatttatt tattaagatt tatttattta ttacatgtag gtacactgta 
     7681 tctgtcttca gacacaccag aagagggcat cagatctcgt tacagatggt tgtgagtcac 
     7741 catgtggttg ctgggatttg aactcaggac ctctggaaga acagtcagtg ctcttaaccg 
     7801 ttgagccatc tcaccagccc cgtgatattt tatttaactt actagaatat actgtgttaa 
     7861 aacatatttt aggggattgg ggagatggcc cattctgtaa agtgtctgcc atgtcatctt 
     7921 caggacctgc gtttgtaacc ccagcatgga gaagacagag acagggagac ccctgggctt 
     7981 cctggcaagc tagactagca aatttggtta actaggttca atgagagact cagtctcaaa 
     8041 acataagaag tgaatttttt agacccaatt tcaatctctg aactccatac gtatgcacat 
     8101 gtacctgcat ataaatatat atacacaccc ataaacacat gcacacacaa aaaatggttc 
     8161 aaaagttctt aaaatgtata agagtagaaa gtgtagagcc gggagtggtg gcgcacgcct 
     8221 ttaatcccag cacttgggag cagaggcagg tggatttctg agttcgaggc cagcctggtc 
     8281 tacagagtga gttccaggac agccagagct atacagagaa atcctgcctc aaaaaaccaa 
     8341 aaataaagtg tatatactaa tctttcatgc ctaaaatatt tatttttgcc acatttttca 
     8401 tacctcttta atatttttct ttttctcttt gtgcttcaaa ggaaattttg tgtcatgttt 
     8461 tacttttcat tcttgactct attcagcagt aagacgcagc atggctttct tagagaagta 
     8521 taatctatta caaaacggta acatttaact gctctacata ccctcttgta gctaaattcc 
     8581 tctgttaaac taagacttat tatctaaacg taattaaatg ttgatatctt ataatgctcc 
     8641 actttgaaac agctgtcttt cttttgcaga ttagcgatga tgaaccaggt tatgacctag 
     8701 atttgttttg tatacctaat cattatgccg aggatttgga aaaagtgttt attcctcatg 
     8761 gactgattat ggacaggtta gtaagacctc gattgaagtt atttttagag ggtgtgtgtg 
     8821 tggtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtaagagaga gaggagaggc 
     8881 atgtgtgata tatatgtgaa gtgtattgca tgtacatgga atccagagaa ggacattagg 
     8941 tgtcctactc tgttgctctc ttattccctt gaatcagtgt ctcttgctga acctggggca 
     9001 tacatactga tttggctagg ctgcctggct agcaagaccc agggatctgc ttgtctctgc 
     9061 cccccagtgc tgacgttaca agcaggtgca accacatctg gtttttatat gggtactggg 
     9121 ggatctgaac ttagggcctg atgcgtgccc aagtgctctc acccgccaag ccacctctcc 
     9181 agccaaaggg ttttttttct tgtattaatt ctaacttagc aactgttgag tagttgatgt 
     9241 ttgaatataa gctaagccaa cttattagaa taaatgtttt ctatttttta cttgtatgtg 
     9301 tgagtgcctg catgtatgta tgtgaagcat atgtattcca ggatgtcaga attgtgatgg 
     9361 attccctgga actggagtta cagaccattg tgaaccactg tgtatgggtg ctgggaactg 
     9421 aactttaggt cctctgcaaa aacagtaagg atcttaactg ataaactatc tttctagccc 
     9481 taaggctttc tacttttaaa gttcgtttct ctttctctct ctctctctct ctctctctct 
     9541 ctctctccct ctccctccct cctcctcctc ctcctctttc ccccctctcg cctcctcttt 
     9601 ctctctcccc tccctctctc tctctcacac acacacacag acagagagag gagagagaaa 
     9661 ggaagcctta ctaattaatt acctagtttt agacataact agtgggaatc catagtaagt 
     9721 aaatcaacat tcttgaagag aggttagaag aattggctct cgttgtccat cgaggctgta 
     9781 taagattatt cttctgtttt ctcagcttac catacatatt ttctacgtag acaataccaa 
     9841 ttcaaataca ctgtacgtaa agtatatgcg cacacacaca cacacacaca cacacacaca 
     9901 cacacaagtt acatattgtc atatagtact aaaatttatg gactttttca tattaataca 
     9961 tctagagcta tcatgttttt tatcttttaa aaattacttt acggggctac agagatgtct 
    10021 cagcagttag gagcactgac tgctcttcca gaggtcctga gttcaattcc cagcaaccac 
    10081 atggtggctc acaaccatct gtaatgggat ctgatccact cttatggtgt gtctgaagac 
    10141 agtgacagtg tactcatata catgaaataa atctttaaaa aattacttta ccatcagggt 
    10201 atatatatat atatatatgt gtgtgtgtgt gtgtgtgtgt atgaagtatt tgctatttga 
    10261 gagggtctca ctgtttagct cagtctggct tggaacgttc tgtatagccc agactggcct 
    10321 caactcagca gtccccctga aaggctctca tatgtggaga ttacaggtat gtgctactgt 
    10381 gcccagccct acatgttact ttgtttttga aatagggtat caaatagctt agactggctt 
    10441 ctaagtcagt gtgtagctaa ggatggtctg agcttctgaa cctgctactt caactcctgg 
    10501 tgtgccatca tggccagttt gcacagttgt gagagtagaa ctcaggacct cctatgtgtt 
    10561 actagacatg ttctctacca gctgagctac atcccaattc gcctacattt acttttattt 
    10621 tttaaaagat ttatttatgt atacagcact ctgcctgcat gtacatctgc atgccagaag 
    10681 agggcaccag atctcatcat acatagttgt gagccaccat gtggttgctg gggattgaac 
    10741 tcagggcctc tggaagagca gtcagtgctc ttaacctctg agccatctct gtagccccct 
    10801 acatttattt taaagattat aggctattct attcatagat gtgccataac tgatttaacc 
    10861 agatcttcat tattttgtac ttagtagagt atattagagg gaatgaatct taaatacaca 
    10921 ttttgacact tgctcagctt tagacaagta aaaatcactg gtcaaggaaa tggtgcttag 
    10981 tggatgcttc tgaactgtca aattgtcctc caacaagtta catcatttta tacttccaac 
    11041 gttatgtgag agttcatttt tctttaggct cttctagtat tttatatttg agaaataaaa 
    11101 tttaaccaaa atttgccaat ctagtaagct tgtttttatt tgtatcttta aattttatga 
    11161 tagatgttat agtgtactct cctctcccct cccctcccct ctcctcctca gcaggatctt 
    11221 gttatgtagc cctaagggat ctaatactta ctctgtagtc caggctgtcc ttcagctcat 
    11281 gagacacacc atcatgcttg gcatgtttct tgttctgaaa ggtatctggt tttaattttg 
    11341 gttgagctgg tgtgttggtg tatatctgta atctcagcac aggcaagtag agacaaggat 
    11401 tatgattaca gtccagcctg ggctatttag agggattctc tttaatcttt cctcatgccc 
    11461 caaaatctta cctttggtat atgaaaaata gtctccactt ctgcaaaata ttgctttatg 
    11521 aagtaagaat taattccctt catagagaca aggaatgtgt cctgtaaaag tttaatgtgt 
    11581 aagaagtatt tgttataaaa gataaatatt cagaatcttc tttttaattc ctgattttat 
    11641 ttctatagga ctgaaagact tgctcgagat gtcatgaagg agatgggagg ccatcacatt 
    11701 gtggccctct gtgtgctcaa ggggggctat aagttctttg ctgacctgct ggattacatt 
    11761 aaagcactga atagaaatag tgatagatcc attcctatga ctgtagattt tatcagactg 
    11821 aagagctact gtgtaagtat aattaactta taattaaaaa aatagggcca ttctagtttt 
    11881 atctatattt tttttaaact tgtgcaaact atgctacata atcaacttac ttgattatgt 
    11941 aatatatata gtaatagttg gaattactat tattacatat ttatttttct gtgctggggt 
    12001 agaacccagg gaactaagta ctgtaataac cgagcttcat tcatcctagt catttttaat 
    12061 tttttttttt ttttttgttt tttcgagaca aggtttctct gtgtagccct ggctgtcctg 
    12121 gaactcactc tgtagaccag gctggcctcg aactcagaaa tctgcctgcc tctgcctccc 
    12181 agtgctgaga ttaatgacgt gcaccaccac tgcctggcat ttttttaaag ttttaatttg 
    12241 agaccctttc tcactaactt gctcacacta tcctcgaact cattacgtgg gctggtctag 
    12301 aaatttcagt caacttgcct cagcttctca agtagctggg attacaggca ggtgctacca 
    12361 cttctggcca tgatcattgt tgttgttgct attattatta ttattatttc actgatactt 
    12421 catatcacaa cacagaatag taactgtagt tcatgaccaa caaaaaagtt gtacagtttc 
    12481 tctgttgtct tattttgatc tggattttga tttgtttcat ggcactgggc aggctaatct 
    12541 caaactgcat ggctcaaata gtccttctgt cttaatctcc catgttctgg aagtataggt 
    12601 atcactacac ctagctgtat tttcatattt taaaaaaaac gcctaagaat tgctattgaa 
    12661 tgaggcggag agatggctca gcagtcacaa gtacttgtag aggactgggg tttggttact 
    12721 agcacctcaa agatggctta caattatctg tagctccaat tccagaggat ctgtgtggta 
    12781 aagctgcatg tatatgggca tgcaggtaaa actgctaaac atataaaaat agctcagtct 
    12841 taagagaatt ggtcgacttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg 
    12901 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata 
    12961 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa 
    13021 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg 
    13081 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga 
    13141 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat 
    13201 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca 
    13261 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc 
    13321 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca 
    13381 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg 
    13441 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca 
    13501 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata 
    13561 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag 
    13621 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg 
    13681 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca 
    13741 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta 
    13801 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct 
    13861 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca 
    13921 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag 
    13981 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat 
    14041 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt 
    14101 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 
    14161 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 
    14221 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccaggt 
    14281 cgagtaggga taacagggta atggatcctc gttgggcacc tgcactcatg tacgcaggcc 
    14341 cacatataga tgtacacacg catatcattc aaaatagaaa ttatttatac aagtagtgcg 
    14401 ttggagagct ggctcaggag ttgttaatgc ttgctaccct tgcaaaggac ccaagttcaa 
    14461 tttccagcac ctgtgttggg catctcacaa ccacatgtaa atctagtttc aggggatttc 
    14521 acccctcttc tgaactcttc aggcacttat acccacaaat ggacatctcc acatagagac 
    14581 acatactttt aaaaaagtaa tctagggtct agagaaatgg ttatagacca cggactggga 
    14641 tcagttccca gaagccacat cctgtggctc acaattgcct gtgaatcaag ttctagatct 
    14701 ggtggcctcg tctagcctgc agtcatcaac aaacacatgc acacatccat acacacataa 
    14761 tttaaaagaa agcgaaagta acctataatg ctaaacaaag cacattctca gtgtagactg 
    14821 cagtggtgat gaaccttgaa aatatattaa tagaagccag acacgtaagt cacaaatggt 
    14881 ataactcaat ttatatgaaa ttcccagaga gtccaaagtt aaaaaaaaca aaaaggcaga 
    14941 ttattggttg tcagtagcta cagagatggg acattgaaga tattaatagg tatagggttg 
    15001 aggattcatg gcctttccct cttccatgtt agcatgtcta ttgttgttaa ccttattcaa 
    15061 ctcatgttag ggcaatcata ttgatgagac ttaatgagta tagcttgaca ttcctagaag 
    15121 acagagtctc acatcagact ctctgatcct ctgactctta gaatctttca acaccctctt 
    15181 ctgaaatgat ctctgagcct ttggttgtga ctgaattatg agaaagaatt ttatgttgaa 
    15241 tgaattttac ttcagcattt ttacaaaact gaagctgggg atggcgtttc atgcgtgcag 
    15301 tccctaccat ttgcctataa gcctggagga ggcagaagta gaattaggct tttcagggac 
    15361 atcttcagct gcatcatgag cttgaggaca acttgagcta cataagattc ccatccccac 
    15421 ccccacacac acacacacat ctagtatgta cctagtatgc agtcttttca tatgtttaaa 
    15481 atgtagaaag gtatatgtga agtctccctt ttccccacaa cttagtccct ccaaagtatt 
    15541 gcatcagttt ccttgtacat gattttatgc atatataaga tacactattt ctctctctct 
    15601 ctctctcctt ccctccttcc ctccaacctc tgggtatgag ttgagggtta tgtatgtttc 
    15661 aagttgtaat aagaattgcc actcaaacat ttcatcccat tttaagttaa gtttgtctga 
    15721 ttttccactt tagaagggca gccatgatgg ctcatgccta taatcccatc ctagcacttg 
    15781 taagcctaat acataagact ggaggattgt catcagcttg aggctagcct ggtctaccta 
    15841 atgagttcca gccagccaag agtagaatat aagaccctgt ctccaaaaaa tgtaccaaag 
    15901 gggctggagg ggtaactcag tggttaagaa cagtagctgc tcttccagag gacctgggtt 
    15961 caattcccag cacccacatg gcagcttgca actatctgta actgtccagc tttgggatat 
    16021 ccgacaccat tataccaatt acataaaatg aaaaaattgt aaaatgtgct aaaatgagtg 
    16081 gggaggggga gcatggtgtg gttctgtggg cttgggattt tgccatgaat ccatgactat 
    16141 atctcagcaa tatgtggatt atttctggaa acctgtgggg catgggtgta tgggaagagg 
    16201 ggagagagag agagcccgca tccagccaga gttcctgtgc tctgggcagg cggacgtggg 
    16261 aggactgctg gacgatttcc acttggccct gggtgggcat ctggctgtgt gaagccactg 
    16321 accccactca gcggggggta gataaggggc atttcccata ccaggttctc agactccagc 
    16381 attccacgct ggatacagca gaagagctga gaacagaata ggggccccag ggagacactc 
    16441 tcgtcccaga gataaagaag gcagagggag agagattcct acgcaggcga gaggccttag 
    16501 tctgggcctt gatgaacaga gacggtctat ggttttagag ctttattgta gaaaggcagg 
    16561 gggaaagaga gaaggtagaa agagagagac cagccatggc caagaggaga gaagggggga 
    16621 aaggagagag agaagaaagg ctagagagta agaaaggtta aagagacaga agtaaaagct 
    16681 tagagtaaga gagagagaga gagcaagagg gagagtaaga aaagtaaaag cttaagagag 
    16741 cgaggagggg ccagcccctc taatagtggg cttgttatct tgttgttgct aggtaactgt 
    16801 gaagcgtcta gcctgaaggc cagaagcttg ggacattgtc tatgtgactg aaagccatga 
    16861 gcctctactg tgggggctgt ggaggtggta acttggacag gagccagggg ttccaggaga 
    16921 catgagggaa tgccttctgt cccatgtagg taggaattac caccaccggc tttcaccgta 
    16981 gttcactgga gactgtttgt acatagccca ttgccccaca gaaaccaggc actcactgtt 
    17041 tcatttgcat aagaattaga aggctttatt ttggctggga ttggtgactt gccccagaaa 
    17101 ttccagcttt tgggaagccg aatcctggag aaccaggagt tcaaggtcat cctcaggtac 
    17161 ctcatttgtt caagaccagc ctgtgctacc atgagacctt gtccctactc ccctcaaccc 
    17221 caaaacacgc tgttttcatt ttataggttg tactaaccat ggaggatggt catttgcctt 
    17281 tctcttgatt tcagaagcgt agcgcgggct ttgcaccatg atctagtgtt tccagatagg 
    17341 cttgcctctc cacagccagg cttatcctgc ttcagtccca tgtttggcaa gggcaacata 
    17401 tgactgaaga acttgcatag ccttatggtg tatcatgact cttagcccca gtttataact 
    17461 gtctaacagc tggtggcttt tgtgaaaaag gaaagcatca tttgaacttt taacttttca 
    17521 gctgtttgtt ttgactttgc ataaatactg agactagcta ggaaatgagc ttttgtttca 
    17581 tttgctaaaa atgatttttt tttcatgagg caatttcaag caatttgaat ttagcaatct 
    17641 ctacttgtct gaaaagaagt tattaattaa aaaaaaacaa acaaacagga acctattatg 
    17701 ctggctagtc acatgaaaga atccaaatga acacagtcaa ggctgaatca acacatgaaa 
    17761 ttattttcta attccccaaa ttacatttat gctaattagt atgtagtaaa acattttaat 
    17821 tgtttccaat gttaaacatt acagaaataa ttttgatgtt ttcctaataa cccagccttt 
    17881 gatcatgttt gcatttattt gattgttgtc agtttattaa ataattaagt aagagcaaca 
    17941 agataaaatg acttcatgag agtccttttc tgtaacttag aacgtcagta gtcataggaa 
    18001 ctgcggtcgt tcccttacct ttcagaagta ggctaaagag ttgaacgcaa aggtggtttt 
    18061 tcgaatcttc ctgatcctta agcccccact aggtttatat tctatatttg atatcggatt 
    18121 cgaacccctc gagcttagga tccggaaccc ttaatataac taagttatta ggtccctcga 
    18181 agaggttcac tagtactggc cattgcggcc gcttcgaggc tgcctcgcgc gtttcggtga 
    18241 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 
    18301 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 
    18361 cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca 
    18421 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta 
    18481 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 
    18541 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 
    18601 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 
    18661 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 
    18721 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 
    18781 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 
    18841 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 
    18901 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 
    18961 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 
    19021 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 
    19081 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 
    19141 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 
    19201 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 
    19261 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 
    19321 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 
    19381 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 
    19441 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 
    19501 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 
    19561 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 
    19621 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 
    19681 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 
    19741 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct 
    19801 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 
    19861 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 
    19921 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 
    19981 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 
    20041 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa 
    20101 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 
    20161 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 
    20221 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 
    20281 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 
    20341 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 
    20401 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg g 
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