LOCUS       Ple87\(pEMS1416)       18553 bp    DNA     circular     11-FEB-2010
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|545678892|
COMMENT     VNTDBDATE|545678892|
COMMENT     LSOWNER|
COMMENT     VNTNAME|Ple87 (pEMS1416)|
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COMMENT                (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #141=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 
COMMENT                1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #142=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 
COMMENT                1 1 "@N" 0) 
COMMENT          #143=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 
COMMENT                5 "@N (@S)" 0) 
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COMMENT                "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 
COMMENT         (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 
COMMENT          (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 -365) 
COMMENT        1 1 0 1 1 
COMMENT        (mapper: 23.4633 -17.8935 40.0378 40.0378 0.01 10 -1 18553 18553 1 0 0) 
COMMENT        #145=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil -457 100) 
COMMENT              (CObjectList 
COMMENT               #146=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -1364 100) 
COMMENT                     (CObjectList 
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COMMENT                            (LOGFONT 58 22 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 
COMMENT                            1.74233 0.833333 0 "Ple87 (pEMS1416)" "@N" 1 0 
COMMENT                            0.871165 0 -10 10.7149 1.47361 Nil) 
COMMENT                      #149=(CLabel 
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COMMENT                            (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 
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COMMENT               #150=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -108 100) 
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COMMENT                                   -12.1573 -6.32595 0 -10 3.02214 1.17389 #152#) 
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COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.51207 1.9 
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COMMENT                                   "MFRT (F5mutant)" "@N" 1 12.9061 10.8656 0 
COMMENT                                   -10 7.91752 1.17389 #154#) 
COMMENT                             #156=(CScratch 
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COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.50058 1.9 
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COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
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COMMENT                                   "Kozak consensus" "@N" 1 13.7555 9.09833 0 
COMMENT                                   -10 7.99245 1.17389 #156#) 
COMMENT                             #158=(CWideArrow 
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COMMENT                                    5.49821 1 0.082322) 0.8 1.8 0) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
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COMMENT                                   11.0837 6.90643 0 -10 2.67247 1.17389 #158#) 
COMMENT                             #160=(CScratch 
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COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.25476 1.9 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Tetracysteine residue" "@N" 1 18.4132 
COMMENT                                   4.16504 0 -10 10.0405 1.17389 #160#) 
COMMENT                             #162=(CScratch 
COMMENT                                   (CWidget 0 (3 #10# 0) 1 2 0 0 #114# 674 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.24259 1.9 
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COMMENT                                   14.6963 2.57257 0 -10 1.99811 1.17389 #162#) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                   14.4006 0.905168 0 -10 2.72243 1.17389 #164#) 
COMMENT                             #166=(CScratch 
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COMMENT                                    100) (LOGPEN 0 8 13395507) 10 1 5.15299 1.9 
COMMENT                                   0.082322 1) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 early polyA signal" "@N" 1 17.2066 
COMMENT                                   -1.71134 0 -10 11.0645 1.17389 #166#) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                   "Ple87 (GCHFR-C)" "@N" 1 10.7022 14.0256 0 
COMMENT                                   -10 7.79264 1.17389 #168#) 
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COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "Region 4" "@N" 
COMMENT                                   1 7.18368 15.5681 0 -10 4.19603 1.17389 #170#) 
COMMENT                             #172=(CWideArrow 
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COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                                    5.88096 1 0.082322) 0.8 1.8 0) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                   "@N" 1 10.6205 12.4831 0 -10 6.16917 1.17389 
COMMENT                                   #172#) 
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COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #175=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Region 3 (reverse complement)" "@N" 1 
COMMENT                                   -7.55346 14.3632 0 -10 14.5862 1.17389 #174#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #176=(CGroupWidget 
COMMENT                            (CWidget 22 (7 22 0) 1 2 0 0 Nil -178 100) 
COMMENT                            (CObjectList 
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COMMENT                                    (CWidget 0 (3 #1# 0) 1 2 0 0 #122# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 1 
COMMENT                                    0.00202873 0.415214 1 0.082322) 0.8 1.8 1) 
COMMENT                             #178=(CLabel (CWidget 0 (0 0) 1 2 0 0 #106# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "AmpR" "@N" 1 
COMMENT                                   -4.56171 12.1963 0 -10 2.87229 1.17389 #177#) 
COMMENT                             #179=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #2# 0) 1 2 0 0 #122# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 0.835356 
COMMENT                                    2.08385 2.56601 1 0.082322) 0.8 1.8 0) 
COMMENT                             #180=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #106# 1196708948 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "AmpR" "@N" 1 
COMMENT                                   -10.5285 -8.71165 0 -10 2.87229 1.17389 #179#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #181=(CGroupWidget 
COMMENT                            (CWidget 86 (7 86 0) 1 2 0 0 Nil -387 100) 
COMMENT                            (CObjectList 
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COMMENT                                    2.0754 1 0.082322) 0.8 1.8 0) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "5' homology sequence" "@N" 1 -16.4575 
COMMENT                                   2.34176 0 -10 10.4901 1.17389 #182#) 
COMMENT                             #184=(CWideArrow 
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COMMENT                                    (CWidget 0 (3 #3# 0) 1 2 0 0 #110# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                             #185=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "3' homology sequence" "@N" 1 5.8167 -12.1963 
COMMENT                                   0 -10 10.4901 1.17389 #184#) 
COMMENT                             #186=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #4# 0) 1 2 0 0 #110# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 3.79711 
COMMENT                                    5.13405 1 0.082322) 0.8 1.8 0) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "HPRT complementary sequence" "@N" 1 18.3286 
COMMENT                                   -5.22699 0 -10 14.9609 1.17389 #186#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #188=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -248 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #189=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -317 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #190=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -806 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 
COMMENT        (CObList #191=(AnlzFontBackColorItem 341 1159 50331392) 
COMMENT         #192=(AnlzFontBackColorItem 1160 2242 33554687) 
COMMENT         #193=(AnlzFontBackColorItem 138 340 33619967))) (CObList) 1699618931 
COMMENT       (CStringList) 0 0 (CObList)))
FEATURES             Location/Qualifiers
     misc_recomb     12416..16227
                     /vntifkey="86"
                     /label=5'\homology\sequence
     misc_marker     complement(17326..18547)
                     /vntifkey="22"
                     /label=AmpR
     misc_marker     10965..12390
                     /vntifkey="22"
                     /label=AmpR
     misc_recomb     7324..10969
                     /vntifkey="86"
                     /label=3'\homology\sequence
                     /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3"
     misc_recomb     3370..7323
                     /vntifkey="86"
                     /label=HPRT\complementary\sequence
                     /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2"
     misc_feature    12397..12414
                     /vntifkey="21"
                     /label=I-Sce\I
     misc_feature    2252..2285
                     /vntifkey="21"
                     /label=MFRT\(F5mutant)
     misc_feature    2286..2296
                     /vntifkey="21"
                     /label=Kozak\consensus
     misc_feature    2293..3012
                     /vntifkey="21"
                     /label=EGFP
     misc_feature    3013..3048
                     /vntifkey="21"
                     /label=Tetracysteine\residue
     misc_feature    3049..3121
                     /vntifkey="21"
                     /label=NLS
     misc_feature    3127..3160
                     /vntifkey="21"
                     /label=MFRT
     misc_feature    3314..3364
                     /vntifkey="21"
                     /label=SV40\early\polyA\signal
     misc_feature    139..2243
                     /vntifkey="21"
                     /label=Ple87\(GCHFR-C)
                     /note="1306"
     misc_feature    342..1160
                     /vntifkey="21"
                     /label=Region\4
     misc_feature    1161..2243
                     /vntifkey="21"
                     /label=Region\Prom
     misc_feature    139..341
                     /vntifkey="21"
                     /label=Region\3\(reverse\complement)
BASE COUNT     4837 a      4296 c      4341 g      5079 t 
ORIGIN
        1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 
       61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 
      121 cgagctccta ggccggccag agctcgctga gtcccggaag ccgagctgag cccgcttctc 
      181 cactccctct ccccggccaa gggggcgtgt ctgggcgggt tcccggtcca gtccccggtg 
      241 ggtgcagccc aggagggagg catgaaggtg cagtcgcagc cccctagggc ctggtcccgg 
      301 ctccaagtcg ccaggcggcc ggtactcacc atgcggatct gaagctttcc acatccttac 
      361 tggaaccgca gaaacagagt ccgttccctg ggtctaggac tccactagca gactgttcct 
      421 tgccctgccc tctcctaagc tggaccccat caaggacaga caggcacaga gagaaaggtt 
      481 tttgtcgtga ggatctgcat gagtcgcaaa gctaagccct agaagcaggg agcagggagc 
      541 ctgcgtccta cctccagggt ggctgctgcc tggggaccta gccatttccc ttggcattct 
      601 tcctaatgcc tccatgtcta aactgtgact actgcttcca ttcctcctct cacccctgga 
      661 ggcagggaga acccaagcta cacaggctct ctgaaaagag ttgtcagagt gagtgccagt 
      721 tcaaatcggg gaaagaggag ggctgatccc agcccctggg tcaaggctcg gggacagaag 
      781 ccagccccct ccctttgcca tgaggcactt gcctctccat gcccagcttg gggcttgagc 
      841 cctttgtctg cttcatggtc tttcagcatc aattaaacct gacagaagga ctaccagcac 
      901 tctccatgct agggagtggc gcacagagcc aggagctact ggggctcagc cttgttgcta 
      961 cagaactgca ggacagccag ggcagtgagg ggcctggagg cttatttttt tttgaaatgg 
     1021 agtctctgtc gcccaggctg gagtgcggtg gtgcaatcat ggctcactgc agcctcagcc 
     1081 tcctcctacc tggaagcctg ggtggggagc aagcccaggg agggcggcag cagggctgtg 
     1141 gtcagactaa catggtctac gctccggcct ggacaataag agcgaaactc catgtccaaa 
     1201 aaaaaaaaaa agaaaagaaa aaagaaaacc aatcacaaca aggcagataa agaaggatga 
     1261 gttgtcagat tttgataact gcggatgagg ggcgtggaca cacggttggg aactcgcagc 
     1321 cgctcattgg tggctggtgc gggggaagtt gtttcatttg ccccaggtgt tgcccagtga 
     1381 cctcctgcgc gtgcgccgcg tctgtccagc tgcgcgggaa gctcagggga gggaaggcca 
     1441 ggttccatcc attcagtcac tccagacctg gcacccagcc aagcgcgcgg ttcctttaca 
     1501 gactttcctg cgggtccaca gaaatgcaag gatccgcctc tgtgctggaa ttagagtctg 
     1561 ggagggggaa tgcacagtgc gccctgggtg gtattttagt tgtcgccttc agggctagct 
     1621 ccgagcctcg tgccccggcc tcctcagcgc ttaaggaatc tctggagcgc atgtcttggc 
     1681 gaggggaagt ggggccccgt tgaagctgtc gaggcgtctc tgagaggggt gggctggggc 
     1741 ctggagaggc ctccgtgagg acgccgtttg ctctgggctc tgcctggcag gggactttgg 
     1801 aacctggcct gaagtgttca agaagcgggc gcgggggcaa cacgcgcgcg gggacgcagt 
     1861 aagatcacag gctgcaaaga aaacagcact aagcgtcctg gaggacgctg gagcttcagg 
     1921 atagtgtcct tccaggtcgt gggaccgccc cctcctcccc gcactccacc ttcccgcaac 
     1981 tggatcactc ggcggaagcc tcaggtgggc cccggggtcc aagtccgccg ccgggagggc 
     2041 gggggcgggc cccgaaccgc cgactgcagg gcgcgtcacg gcagccgctc ctgattggcg 
     2101 gccacggcgg tcacgggcaa ggtgccccgg gattgacgcc gcctgtggcc agagccggag 
     2161 taacgggact cccagctgcg cgtcgcagtc ccgacgcgag aagggctgga gtcggcgtcc 
     2221 agcctagagc ccccggtggg accggcgcgc cgaagttcct attcttcaaa aggtatagga 
     2281 acttccgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 
     2341 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 
     2401 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 
     2461 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 
     2521 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 
     2581 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 
     2641 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 
     2701 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 
     2761 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 
     2821 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 
     2881 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 
     2941 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 
     3001 ctgtacaagt tatttctgaa ctgctgcccg ggctgctgca tggaaccgga tccaaaaaag 
     3061 aagagaaagg tagatccaaa aaagaagaga aaggtagatc caaaaaagaa gagaaaggta 
     3121 atataagaag ttcctattct ctagaaagta taggaacttc agcggccgcg actctagatc 
     3181 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc 
     3241 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct 
     3301 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 
     3361 ctgcgaattc tcgtaaaact cttcatggca gtagttatta ttctctctct ctctcttttt 
     3421 cttttttctt gagacaggat atttctctgt tgcccaggct ggagtgcagt ggcacagtct 
     3481 tggctcactg cagcctggac ctcctgggct caagccatcc tcccacctca gcctcccaag 
     3541 tagctggggc tacaggcaca tggccaccag gcccagataa tttttcattt ttgtagagac 
     3601 tgagtctcac catgttaccc aggtttatta ttctcatttt ttagatgaag agactgaggt 
     3661 ccagagaagc tcaatgactt gcctagtttt acaaatctcc tgccatcaca tacccctcag 
     3721 cgtccttaat aagagggagg ccaccaacta tgtgctgggc actgtggtgg atgctggagc 
     3781 tatagggttg agtatataag aaatggtgtt gctggagcaa ctgttgcttg cttacctgac 
     3841 ctatctgaga attaattagc aggggaacat atttttgttt tcagattcaa tataagaact 
     3901 tgtgtgggca aaaataaaga tcagtagtaa taacagtagt tcccatttgc tgactgtact 
     3961 gtcctaagtg catatatata tacatacaca cacgcatacc tatactcctc taatactcaa 
     4021 aatgatcctg tttatgtatt gttaatatgc tcattttatt tttaaatttt tatttatttt 
     4081 tatttttatt tatttttgag acggagtctc attctgtcgc ggaggctgaa gtgcagtggt 
     4141 gcgatctcag ctcagtgcga cctccgcctc ccgggttcaa gtgattctcc tgcctcagcc 
     4201 tccggactag ctgggattac aggcgcccgc ctccacgccc agctaatttt tgtattttta 
     4261 gtagagatgg ggtttcgcca tgttggccag gctggtctcg tactcctgac cttgagtgat 
     4321 ccacctgcct cggcctccca aagtgctggg attacaggca tgagccaccg cgccgggcta 
     4381 atatgctcat tttagtgagg caaaaataga ggctcagagt ctgatttgta caaaactaca 
     4441 gagcagttaa gtgtcctctc agatgtgtac cctgatctgg gtgactctag gactctaggt 
     4501 ctcaactgtt acaaccagtt aagggtttgg ggaagcactg ggccaagagt caggaaaatg 
     4561 gaagccacag gtagtgcaag gtcttgggaa tgggacgtct ggtccaagga ttcacgcgat 
     4621 gactggaacc cgaagagccg gggcccggtt tacggccgcc atgaagcaac gcgcgccggt 
     4681 aggtttggga atcagggagc cctctgaata ggagactgag ttgggaggga aaggggcttc 
     4741 gctgggggag cctcggcttc ttctgggaga aaattcccac ggctacctag tgagcctgca 
     4801 aactggtagg cgccggcgta ggcgcgcggg cggggccggg ggcggggcct gcggggcgtg 
     4861 gcggggcggg cagagggcgg ggcctgcttc tcctcagctt caggcggctg cgacgagccc 
     4921 tcaggcgaac ctctcggctt tcccgcgcgg cgccgcctct tgctgcgcct ccgcctcctc 
     4981 ctctgctccg ccaccggctt cctcctcctg agcagtcagc ccgcgcgccg gccggctccg 
     5041 ttatggcgac ccgcagccct ggcgtcgtgg tgagcagctc ggcctgccgg ccctggccgg 
     5101 ttcaggccca cgcggcaggt ggcggccggg ccctgaggcg cgggatcgat ccatgacacc 
     5161 cccccgcccc ccatatctca ctataattca ctgtcaaatg gcttttatga tttggagttt 
     5221 ggggatactt ttaaagacag ggtcttggta gctcctagat ggcttgggat tcacttagta 
     5281 acccttgctg gcctaaaatt caggaacttc tacctcatct tcccaagtac tgggttagta 
     5341 gggatgtcca gcatatttag cttgctttaa tgattttgct gtactttttt ctgtgtgtac 
     5401 aggtgcatgt atatttataa aactaacata atatatttta cccccaaagt accataaccc 
     5461 taattaatgc tattaaatgt tttgtatgct gggctctggg catactaata taaccaatat 
     5521 tatacaatgc aaaacaattt tggaatatct tctgtaaaat tatcttatta gtaagttgta 
     5581 accatctcag acttctaagt ttgagtttgt aaaggatcag ttttttttgg tgacctacac 
     5641 acacacacac acacacacac acacacacac acacaggggg gtggattctg agaatttaga 
     5701 gaatttagat ggttaatacc agtttttatt tttattctgt gtgatattta tttattaaga 
     5761 tttatttatt tattacatgt aggtacactg tatctgtctt cagacacacc agaagagggc 
     5821 atcagatctc gttacagatg gttgtgagtc accatgtggt tgctgggatt tgaactcagg 
     5881 acctctggaa gaacagtcag tgctcttaac cgttgagcca tctcaccagc cccgtgatat 
     5941 tttatttaac ttactagaat atactgtgtt aaaacatatt ttaggggatt ggggagatgg 
     6001 cccattctgt aaagtgtctg ccatgtcatc ttcaggacct gcgtttgtaa ccccagcatg 
     6061 gagaagacag agacagggag acccctgggc ttcctggcaa gctagactag caaatttggt 
     6121 taactaggtt caatgagaga ctcagtctca aaacataaga agtgaatttt ttagacccaa 
     6181 tttcaatctc tgaactccat acgtatgcac atgtacctgc atataaatat atatacacac 
     6241 ccataaacac atgcacacac aaaaaatggt tcaaaagttc ttaaaatgta taagagtaga 
     6301 aagtgtagag ccgggagtgg tggcgcacgc ctttaatccc agcacttggg agcagaggca 
     6361 ggtggatttc tgagttcgag gccagcctgg tctacagagt gagttccagg acagccagag 
     6421 ctatacagag aaatcctgcc tcaaaaaacc aaaaataaag tgtatatact aatctttcat 
     6481 gcctaaaata tttatttttg ccacattttt catacctctt taatattttt ctttttctct 
     6541 ttgtgcttca aaggaaattt tgtgtcatgt tttacttttc attcttgact ctattcagca 
     6601 gtaagacgca gcatggcttt cttagagaag tataatctat tacaaaacgg taacatttaa 
     6661 ctgctctaca taccctcttg tagctaaatt cctctgttaa actaagactt attatctaaa 
     6721 cgtaattaaa tgttgatatc ttataatgct ccactttgaa acagctgtct ttcttttgca 
     6781 gattagcgat gatgaaccag gttatgacct agatttgttt tgtataccta atcattatgc 
     6841 cgaggatttg gaaaaagtgt ttattcctca tggactgatt atggacaggt tagtaagacc 
     6901 tcgattgaag ttatttttag agggtgtgtg tgtggtgtgt gtgtgtgtgt gtgtgtgtgt 
     6961 gtgtgtgtgt gtgtaagaga gagaggagag gcatgtgtga tatatatgtg aagtgtattg 
     7021 catgtacatg gaatccagag aaggacatta ggtgtcctac tctgttgctc tcttattccc 
     7081 ttgaatcagt gtctcttgct gaacctgggg catacatact gatttggcta ggctgcctgg 
     7141 ctagcaagac ccagggatct gcttgtctct gccccccagt gctgacgtta caagcaggtg 
     7201 caaccacatc tggtttttat atgggtactg ggggatctga acttagggcc tgatgcgtgc 
     7261 ccaagtgctc tcacccgcca agccacctct ccagccaaag ggtttttttt cttgtattaa 
     7321 ttctaactta gcaactgttg agtagttgat gtttgaatat aagctaagcc aacttattag 
     7381 aataaatgtt ttctattttt tacttgtatg tgtgagtgcc tgcatgtatg tatgtgaagc 
     7441 atatgtattc caggatgtca gaattgtgat ggattccctg gaactggagt tacagaccat 
     7501 tgtgaaccac tgtgtatggg tgctgggaac tgaactttag gtcctctgca aaaacagtaa 
     7561 ggatcttaac tgataaacta tctttctagc cctaaggctt tctactttta aagttcgttt 
     7621 ctctttctct ctctctctct ctctctctct ctctctctcc ctctccctcc ctcctcctcc 
     7681 tcctcctctt tcccccctct cgcctcctct ttctctctcc cctccctctc tctctctcac 
     7741 acacacacac agacagagag aggagagaga aaggaagcct tactaattaa ttacctagtt 
     7801 ttagacataa ctagtgggaa tccatagtaa gtaaatcaac attcttgaag agaggttaga 
     7861 agaattggct ctcgttgtcc atcgaggctg tataagatta ttcttctgtt ttctcagctt 
     7921 accatacata ttttctacgt agacaatacc aattcaaata cactgtacgt aaagtatatg 
     7981 cgcacacaca cacacacaca cacacacaca cacacacaag ttacatattg tcatatagta 
     8041 ctaaaattta tggacttttt catattaata catctagagc tatcatgttt tttatctttt 
     8101 aaaaattact ttacggggct acagagatgt ctcagcagtt aggagcactg actgctcttc 
     8161 cagaggtcct gagttcaatt cccagcaacc acatggtggc tcacaaccat ctgtaatggg 
     8221 atctgatcca ctcttatggt gtgtctgaag acagtgacag tgtactcata tacatgaaat 
     8281 aaatctttaa aaaattactt taccatcagg gtatatatat atatatatat gtgtgtgtgt 
     8341 gtgtgtgtgt gtatgaagta tttgctattt gagagggtct cactgtttag ctcagtctgg 
     8401 cttggaacgt tctgtatagc ccagactggc ctcaactcag cagtccccct gaaaggctct 
     8461 catatgtgga gattacaggt atgtgctact gtgcccagcc ctacatgtta ctttgttttt 
     8521 gaaatagggt atcaaatagc ttagactggc ttctaagtca gtgtgtagct aaggatggtc 
     8581 tgagcttctg aacctgctac ttcaactcct ggtgtgccat catggccagt ttgcacagtt 
     8641 gtgagagtag aactcaggac ctcctatgtg ttactagaca tgttctctac cagctgagct 
     8701 acatcccaat tcgcctacat ttacttttat tttttaaaag atttatttat gtatacagca 
     8761 ctctgcctgc atgtacatct gcatgccaga agagggcacc agatctcatc atacatagtt 
     8821 gtgagccacc atgtggttgc tggggattga actcagggcc tctggaagag cagtcagtgc 
     8881 tcttaacctc tgagccatct ctgtagcccc ctacatttat tttaaagatt ataggctatt 
     8941 ctattcatag atgtgccata actgatttaa ccagatcttc attattttgt acttagtaga 
     9001 gtatattaga gggaatgaat cttaaataca cattttgaca cttgctcagc tttagacaag 
     9061 taaaaatcac tggtcaagga aatggtgctt agtggatgct tctgaactgt caaattgtcc 
     9121 tccaacaagt tacatcattt tatacttcca acgttatgtg agagttcatt tttctttagg 
     9181 ctcttctagt attttatatt tgagaaataa aatttaacca aaatttgcca atctagtaag 
     9241 cttgttttta tttgtatctt taaattttat gatagatgtt atagtgtact ctcctctccc 
     9301 ctcccctccc ctctcctcct cagcaggatc ttgttatgta gccctaaggg atctaatact 
     9361 tactctgtag tccaggctgt ccttcagctc atgagacaca ccatcatgct tggcatgttt 
     9421 cttgttctga aaggtatctg gttttaattt tggttgagct ggtgtgttgg tgtatatctg 
     9481 taatctcagc acaggcaagt agagacaagg attatgatta cagtccagcc tgggctattt 
     9541 agagggattc tctttaatct ttcctcatgc cccaaaatct tacctttggt atatgaaaaa 
     9601 tagtctccac ttctgcaaaa tattgcttta tgaagtaaga attaattccc ttcatagaga 
     9661 caaggaatgt gtcctgtaaa agtttaatgt gtaagaagta tttgttataa aagataaata 
     9721 ttcagaatct tctttttaat tcctgatttt atttctatag gactgaaaga cttgctcgag 
     9781 atgtcatgaa ggagatggga ggccatcaca ttgtggccct ctgtgtgctc aaggggggct 
     9841 ataagttctt tgctgacctg ctggattaca ttaaagcact gaatagaaat agtgatagat 
     9901 ccattcctat gactgtagat tttatcagac tgaagagcta ctgtgtaagt ataattaact 
     9961 tataattaaa aaaatagggc cattctagtt ttatctatat tttttttaaa cttgtgcaaa 
    10021 ctatgctaca taatcaactt acttgattat gtaatatata tagtaatagt tggaattact 
    10081 attattacat atttattttt ctgtgctggg gtagaaccca gggaactaag tactgtaata 
    10141 accgagcttc attcatccta gtcattttta attttttttt ttttttttgt tttttcgaga 
    10201 caaggtttct ctgtgtagcc ctggctgtcc tggaactcac tctgtagacc aggctggcct 
    10261 cgaactcaga aatctgcctg cctctgcctc ccagtgctga gattaatgac gtgcaccacc 
    10321 actgcctggc atttttttaa agttttaatt tgagaccctt tctcactaac ttgctcacac 
    10381 tatcctcgaa ctcattacgt gggctggtct agaaatttca gtcaacttgc ctcagcttct 
    10441 caagtagctg ggattacagg caggtgctac cacttctggc catgatcatt gttgttgttg 
    10501 ctattattat tattattatt tcactgatac ttcatatcac aacacagaat agtaactgta 
    10561 gttcatgacc aacaaaaaag ttgtacagtt tctctgttgt cttattttga tctggatttt 
    10621 gatttgtttc atggcactgg gcaggctaat ctcaaactgc atggctcaaa tagtccttct 
    10681 gtcttaatct cccatgttct ggaagtatag gtatcactac acctagctgt attttcatat 
    10741 tttaaaaaaa acgcctaaga attgctattg aatgaggcgg agagatggct cagcagtcac 
    10801 aagtacttgt agaggactgg ggtttggtta ctagcacctc aaagatggct tacaattatc 
    10861 tgtagctcca attccagagg atctgtgtgg taaagctgca tgtatatggg catgcaggta 
    10921 aaactgctaa acatataaaa atagctcagt cttaagagaa ttggtcgact ttttcgccct 
    10981 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc 
    11041 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg 
    11101 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt 
    11161 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct 
    11221 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat 
    11281 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg 
    11341 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg 
    11401 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc 
    11461 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat 
    11521 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact 
    11581 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca 
    11641 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact 
    11701 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg 
    11761 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg 
    11821 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg 
    11881 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg 
    11941 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag 
    12001 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc 
    12061 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga 
    12121 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat 
    12181 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc 
    12241 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 
    12301 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 
    12361 gcttgcaaac aaaaaaacca ccgctaccag gtcgagtagg gataacaggg taatggatcc 
    12421 tcgttgggca cctgcactca tgtacgcagg cccacatata gatgtacaca cgcatatcat 
    12481 tcaaaataga aattatttat acaagtagtg cgttggagag ctggctcagg agttgttaat 
    12541 gcttgctacc cttgcaaagg acccaagttc aatttccagc acctgtgttg ggcatctcac 
    12601 aaccacatgt aaatctagtt tcaggggatt tcacccctct tctgaactct tcaggcactt 
    12661 atacccacaa atggacatct ccacatagag acacatactt ttaaaaaagt aatctagggt 
    12721 ctagagaaat ggttatagac cacggactgg gatcagttcc cagaagccac atcctgtggc 
    12781 tcacaattgc ctgtgaatca agttctagat ctggtggcct cgtctagcct gcagtcatca 
    12841 acaaacacat gcacacatcc atacacacat aatttaaaag aaagcgaaag taacctataa 
    12901 tgctaaacaa agcacattct cagtgtagac tgcagtggtg atgaaccttg aaaatatatt 
    12961 aatagaagcc agacacgtaa gtcacaaatg gtataactca atttatatga aattcccaga 
    13021 gagtccaaag ttaaaaaaaa caaaaaggca gattattggt tgtcagtagc tacagagatg 
    13081 ggacattgaa gatattaata ggtatagggt tgaggattca tggcctttcc ctcttccatg 
    13141 ttagcatgtc tattgttgtt aaccttattc aactcatgtt agggcaatca tattgatgag 
    13201 acttaatgag tatagcttga cattcctaga agacagagtc tcacatcaga ctctctgatc 
    13261 ctctgactct tagaatcttt caacaccctc ttctgaaatg atctctgagc ctttggttgt 
    13321 gactgaatta tgagaaagaa ttttatgttg aatgaatttt acttcagcat ttttacaaaa 
    13381 ctgaagctgg ggatggcgtt tcatgcgtgc agtccctacc atttgcctat aagcctggag 
    13441 gaggcagaag tagaattagg cttttcaggg acatcttcag ctgcatcatg agcttgagga 
    13501 caacttgagc tacataagat tcccatcccc acccccacac acacacacac atctagtatg 
    13561 tacctagtat gcagtctttt catatgttta aaatgtagaa aggtatatgt gaagtctccc 
    13621 ttttccccac aacttagtcc ctccaaagta ttgcatcagt ttccttgtac atgattttat 
    13681 gcatatataa gatacactat ttctctctct ctctctctcc ttccctcctt ccctccaacc 
    13741 tctgggtatg agttgagggt tatgtatgtt tcaagttgta ataagaattg ccactcaaac 
    13801 atttcatccc attttaagtt aagtttgtct gattttccac tttagaaggg cagccatgat 
    13861 ggctcatgcc tataatccca tcctagcact tgtaagccta atacataaga ctggaggatt 
    13921 gtcatcagct tgaggctagc ctggtctacc taatgagttc cagccagcca agagtagaat 
    13981 ataagaccct gtctccaaaa aatgtaccaa aggggctgga ggggtaactc agtggttaag 
    14041 aacagtagct gctcttccag aggacctggg ttcaattccc agcacccaca tggcagcttg 
    14101 caactatctg taactgtcca gctttgggat atccgacacc attataccaa ttacataaaa 
    14161 tgaaaaaatt gtaaaatgtg ctaaaatgag tggggagggg gagcatggtg tggttctgtg 
    14221 ggcttgggat tttgccatga atccatgact atatctcagc aatatgtgga ttatttctgg 
    14281 aaacctgtgg ggcatgggtg tatgggaaga ggggagagag agagagcccg catccagcca 
    14341 gagttcctgt gctctgggca ggcggacgtg ggaggactgc tggacgattt ccacttggcc 
    14401 ctgggtgggc atctggctgt gtgaagccac tgaccccact cagcgggggg tagataaggg 
    14461 gcatttccca taccaggttc tcagactcca gcattccacg ctggatacag cagaagagct 
    14521 gagaacagaa taggggcccc agggagacac tctcgtccca gagataaaga aggcagaggg 
    14581 agagagattc ctacgcaggc gagaggcctt agtctgggcc ttgatgaaca gagacggtct 
    14641 atggttttag agctttattg tagaaaggca gggggaaaga gagaaggtag aaagagagag 
    14701 accagccatg gccaagagga gagaaggggg gaaaggagag agagaagaaa ggctagagag 
    14761 taagaaaggt taaagagaca gaagtaaaag cttagagtaa gagagagaga gagagcaaga 
    14821 gggagagtaa gaaaagtaaa agcttaagag agcgaggagg ggccagcccc tctaatagtg 
    14881 ggcttgttat cttgttgttg ctaggtaact gtgaagcgtc tagcctgaag gccagaagct 
    14941 tgggacattg tctatgtgac tgaaagccat gagcctctac tgtgggggct gtggaggtgg 
    15001 taacttggac aggagccagg ggttccagga gacatgaggg aatgccttct gtcccatgta 
    15061 ggtaggaatt accaccaccg gctttcaccg tagttcactg gagactgttt gtacatagcc 
    15121 cattgcccca cagaaaccag gcactcactg tttcatttgc ataagaatta gaaggcttta 
    15181 ttttggctgg gattggtgac ttgccccaga aattccagct tttgggaagc cgaatcctgg 
    15241 agaaccagga gttcaaggtc atcctcaggt acctcatttg ttcaagacca gcctgtgcta 
    15301 ccatgagacc ttgtccctac tcccctcaac cccaaaacac gctgttttca ttttataggt 
    15361 tgtactaacc atggaggatg gtcatttgcc tttctcttga tttcagaagc gtagcgcggg 
    15421 ctttgcacca tgatctagtg tttccagata ggcttgcctc tccacagcca ggcttatcct 
    15481 gcttcagtcc catgtttggc aagggcaaca tatgactgaa gaacttgcat agccttatgg 
    15541 tgtatcatga ctcttagccc cagtttataa ctgtctaaca gctggtggct tttgtgaaaa 
    15601 aggaaagcat catttgaact tttaactttt cagctgtttg ttttgacttt gcataaatac 
    15661 tgagactagc taggaaatga gcttttgttt catttgctaa aaatgatttt tttttcatga 
    15721 ggcaatttca agcaatttga atttagcaat ctctacttgt ctgaaaagaa gttattaatt 
    15781 aaaaaaaaac aaacaaacag gaacctatta tgctggctag tcacatgaaa gaatccaaat 
    15841 gaacacagtc aaggctgaat caacacatga aattattttc taattcccca aattacattt 
    15901 atgctaatta gtatgtagta aaacatttta attgtttcca atgttaaaca ttacagaaat 
    15961 aattttgatg ttttcctaat aacccagcct ttgatcatgt ttgcatttat ttgattgttg 
    16021 tcagtttatt aaataattaa gtaagagcaa caagataaaa tgacttcatg agagtccttt 
    16081 tctgtaactt agaacgtcag tagtcatagg aactgcggtc gttcccttac ctttcagaag 
    16141 taggctaaag agttgaacgc aaaggtggtt tttcgaatct tcctgatcct taagccccca 
    16201 ctaggtttat attctatatt tgatatcgga ttcgaacccc tcgagcttag gatccggaac 
    16261 ccttaatata actaagttat taggtccctc gaagaggttc actagtactg gccattgcgg 
    16321 ccgcttcgag gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag 
    16381 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag 
    16441 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat 
    16501 agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc 
    16561 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt 
    16621 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 
    16681 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 
    16741 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 
    16801 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 
    16861 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 
    16921 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 
    16981 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 
    17041 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 
    17101 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 
    17161 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 
    17221 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 
    17281 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 
    17341 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 
    17401 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 
    17461 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 
    17521 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 
    17581 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 
    17641 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 
    17701 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 
    17761 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 
    17821 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca 
    17881 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 
    17941 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 
    18001 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 
    18061 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 
    18121 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg 
    18181 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 
    18241 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 
    18301 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 
    18361 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 
    18421 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 
    18481 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 
    18541 tgccacctga cgg 
//
