LOCUS       pPK1E-2+t-gfp        5938 bp    DNA     circular     21-AUG-2013
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|670951685|
COMMENT     VNTDBDATE|670951685|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pPK1E-2+t-gfp|
COMMENT     VNTAUTHORNAME|Mibi|
COMMENT     VNTOAUTHORNAME|Schuller|
FEATURES             Location/Qualifiers
     promoter        complement(577..583)
                     /vntifkey="30"
                     /label=Promoter\ß-lactamase
     CDS             complement(814..2187)
                     /vntifkey="4"
                     /label=gene\for\tetracycline\efflux-pump\(B.\megaterium)
     CDS             complement(2390..3391)
                     /vntifkey="4"
                     /label=replicon\repU\(B.\megaterium)
     rep_origin      3543..3569
                     /vntifkey="33"
                     /label=oriU\(B.\megaterium)
     rep_origin      4797..5460
                     /vntifkey="33"
                     /label=ori\pBR322\(E.\coli)
     terminator      4679..4722
                     /vntifkey="43"
                     /label=Terminator\SP6/K1E
     CDS             complement(5623..542)
                     /vntifkey="4"
                     /label=ß-lactamase\(E.\coli)
     promoter        3804..3893
                     /vntifkey="30"
                     /label=Promoter\K1E-2
     CDS             3900..4640
                     /vntifkey="4"
                     /label=gfp
BASE COUNT     1780 a      1313 c      1064 g      1781 t 
ORIGIN
        1 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 
       61 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 
      121 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 
      181 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 
      241 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca 
      301 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 
      361 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 
      421 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 
      481 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 
      541 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 
      601 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 
      661 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg 
      721 cgtatcacga ggccctttcg tcttcaagaa ttcctgttat aaaaaaagga tcaattttga 
      781 actctctccc aaagttgatc ccttaacgat ttagaaatcc ctttgagaat gtttatatac 
      841 attcaaggta accagccaac taatgacaat gattcctgaa aaaagtaata acaaattact 
      901 atacagataa gttgactgat caacttccat aggtaacaac ctttgatcaa gtaagggtat 
      961 ggataataaa ccacctacaa ttgcaatacc tgttccctct gataaaaagc tggtaaagtt 
     1021 aagcaaactc attccagcac cagcttcctg ctgtttcaag ctacttgaaa caattgttga 
     1081 tataactgtt ttggtgaacg aaagcccacc taaaacaaat acgattataa ttgtcatgaa 
     1141 ccatgatgtt gtttctaaaa gaaaggaagc agttaaaaag ctaacagaaa gaaatgtaac 
     1201 tccgatgttt aacacgtata aaggacctct tctatcaaca agtatcccac caatgtagcc 
     1261 gaaaataatg acactcattg ttccagggaa aataattaca cttccgattt cggcagtact 
     1321 tagctggtga acatctttca tcatataagg aaccatagag acaaaccctg ctactgttcc 
     1381 aaatataatt cccccacaaa gaactccaat cataaaaggt atatttttcc ctaatccggg 
     1441 atcaacaaaa ggatctgtta ctttcctgat atgttttaca aatatcagga atgacagcac 
     1501 gctaacgata agaaaagaaa tgctatatga tgttgtaaac aacataaaaa atacaatgcc 
     1561 tacagacatt agtataattc ctttgatatc aaaatgacct tttatcctta cttctttctt 
     1621 taataatttc ataagaaacg gaacagtgat aattgttatc ataggaatga gtagaagata 
     1681 ggaccaatga atataatggg ctatcattcc accaatcgct ggaccgactc cttctcccat 
     1741 ggctactatc gatccaataa gaccaaatgc tttaccccta ttttcctttg gaatatagcg 
     1801 cgcaactaca accattacga gtgctggaaa tgcagctgca ccagcccctt gaataaaacg 
     1861 agccataata agtaaggaaa agaaagaatg gccaacaaac ccaattaccg acccgaaaca 
     1921 atttattata attccaaata ggagtaacct tttgatgcct aattgatcag atagctttcc 
     1981 atatacagct gttccaatgg aaaaggttaa cataaaggct gtgttcaccc agtttgtact 
     2041 cgcaggtggt ttattaaaat catttgcaat atcaggtaat gagacgttca aaaccatttc 
     2101 atttaatacg ctaaaaaaag ataaaatgca aagccaaatt aaaatttggt tgtgtcgtaa 
     2161 attcgattgt gaataggatg tattcacatt tcaccctcca ataatgaggg cagacgtagt 
     2221 ttatagggtt aatgatacgc ttccctcttt taattgaacc ctgttacatt cattacactt 
     2281 cataattaat tcctcctaaa cttgattaaa acattttacc acatataaac taagttttaa 
     2341 attcagtatt tcatcactta tacaacaata tggcccgttt gttgaactac tctttaataa 
     2401 aataattttt ccgttcccaa ttccacattg caataataga aaatccatct tcatcggctt 
     2461 tttcgtcatc atctgtatga atcaaatcgc cttcttctgt gtcatcaagg tttaattttt 
     2521 tatgtatttc ttttaacaaa ccaccatagg agattaacct tttacggtgt aaaccttcct 
     2581 ccaaatcaga caaacgtttc aaattctttt cttcatcatc ggtcataaaa tccgtatcct 
     2641 ttacaggata ttttgcagtt tcgtcaattg ccgattgtat atccgattta tatttatttt 
     2701 tcggtcgaat catttgaact tttacatttg gatcatagtc taatttcatt gcctttttcc 
     2761 aaaattgaat ccattgtttt tgattcacgt agttttctgt attcttaaaa taagttggtt 
     2821 ccacacatac caatacatgc atgtgctgat tataagaatt atctttatta tttattgtca 
     2881 cttccgttgc acgcataaaa ccaacaagat ttttattaat ttttttatat tgcatcattc 
     2941 ggcgaaatcc ttgagccata tctgacaaac tcttatttaa ttcttcgcca tcataaacat 
     3001 ttttaactgt taatgtgaga aacaaccaac gaactgttgg cttttgttta ataacttcag 
     3061 caacaacctt ttgtgactga atgccatgtt tcattgctct cctccagttg cacattggac 
     3121 aaagcctgga tttacaaaac cacactcgat acaactttct ttcgcctgtt tcacgatttt 
     3181 gtttatactc taatatttca gcacaatctt ttactctttc agccttttta aattcaagaa 
     3241 tatgcagaag ttcaaagtaa tcaacattag cgattttctt ttctctccat ggtctcactt 
     3301 ttccactttt tgtcttgtcc actaaaaccc ttgatttttc atctgaataa atgctactat 
     3361 taggacacat aatattaaaa gaaaccccca tctatttagt tatttgtttg gtcacttata 
     3421 actttaacag atggggtttt tctgtgcaac caattttaag ggttttcaat actttaaaac 
     3481 acatacatac caacacttca acgcaccttt cagcaactaa aataaaaatg acgttatttc 
     3541 tatatgtatc aagataagaa agaacaagtt caaaaccatc aaaaaaagac accttttcag 
     3601 gtgctttttt tattttataa actcattccc tgatctcgac ttcgttcttt ttttacctct 
     3661 cggttatgag ttagttcaaa ttcgttcttt ttaggttcta aatcgtgttt ttcttggaat 
     3721 tgtgctgttt tatcctttac cttgtctaca aaccccttaa aaacgttttt aaaggctttt 
     3781 aagccgtctg tacgttcctt aagcaaacta tttagctgac actataagag aaggcttaac 
     3841 aaggcgttgc tacggtagcg cctgattaaa ctttcactta caaggaggtg aaatgtacaa 
     3901 tggtccaaac tagttcgaag atctgggcta gcaaaggaga agaacttttc actggagttg 
     3961 tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag 
     4021 agggtgaagg tgatgctaca tacggaaagc ttacccttaa atttatttgc actactggaa 
     4081 aactacctgt tccatggcca acacttgtca ctactttgac ctatggtgtt caatgctttt 
     4141 cccgttatcc ggatcatatg aaacggcatg actttttcaa gagtgccatg cccgaaggtt 
     4201 atgtacagga acgcactata tctttcaaag atgacgggaa ctacaagacg cgtgctgaag 
     4261 tcaagtttga aggtgatacc cttgttaatc gtatcgagtt aaaaggtatt gattttaaag 
     4321 aagatggaaa cattctcgga cacaaactcg agtacaacta taactcacac aatgtataca 
     4381 tcacggcaga caaacaaaag aatggaatca aagctaactt caaaattcgc cacaacattg 
     4441 aagatggatc cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc 
     4501 ctgtcctttt accagacaac cattacctgt cgacacaatc tgccctttcg aaagatccca 
     4561 acgaaaagcg tgaccacatg gtccttcttg agtttgtaac tgctgctggg attacacatg 
     4621 gcatggatga gccctacaaa taatgaattc gcggccgcat gctaactagc taaccggtta 
     4681 cctatgccct atctaccttg cgtaggtagg gttctttttg ttgccggtcc aagaattgga 
     4741 gctaattaat tcttgcggag aactgtgaat gcgcaaacca acccttggca gaacatatcc 
     4801 atcgcgtccg ccatctccag cagccgcacg cggcgcatct cgggccgcgt tgctggcgtt 
     4861 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 
     4921 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 
     4981 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 
     5041 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 
     5101 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 
     5161 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 
     5221 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 
     5281 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 
     5341 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 
     5401 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 
     5461 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 
     5521 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 
     5581 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 
     5641 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 
     5701 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 
     5761 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 
     5821 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 
     5881 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgca 
//
