LOCUS       pAAV\TBG\FFLuc          5915 bp    DNA     circular     24-FEB-2012
SOURCE      
  ORGANISM  
COMMENT     Component Fragments
              #1: FRAGMENT of <a href="vnti:dnarna/pENN%20AAV%20CMV%20PI?sel=722-207">pENN AAV CMV PI</a> 
                parent position: from 722 to 207
                original length: 3753
                molecule position: from 920 to 202
                Left Terminus
                    HindIII site #1
                      Completely filled in
                Right Terminus
                    SphI site #1
                      3'-5' exonuclease treated (4 bp)
              #2: FRAGMENT of <a href="vnti:dnarna/pAAV%20TBG%20null%20seq?sel=192-908">pAAV TBG null seq</a> 
                parent position: from 192 to 908
                original length: 717
                molecule position: from 203 to 919
                Left Terminus
                    EcoRI site #1
                      Completely filled in
                Right Terminus
                    PstI site #1
                      3'-5' exonuclease treated (4 bp)
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|618692795|
COMMENT     VNTDBDATE|618692795|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV TBG FFLuc|
COMMENT     VNTAUTHORNAME|JunXie|
COMMENT     VNTAUTHORTEL|508-856-3316|
COMMENT     VNTAUTHORFAX|508-856-1552|
COMMENT     VNTAUTHOREML|jun.xie@umassmed.edu|
COMMENT     VNTAUTHORAD1|University of Massachusetts Medical School|
COMMENT     VNTAUTHORAD2|381 Plantation St., Biotech 5, Ste. 250|
COMMENT     VNTAUTHORAD3|Worcester, MA 01605|
COMMENT     VNTOAUTHORNAME|Demo User|
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FEATURES             Location/Qualifiers
     rep_origin      complement(3272..3727)
                     /vntifkey="33"
                     /label=f1\ori
     misc_marker     3858..4715
                     /vntifkey="22"
                     /label=AP(R)
     rep_origin      4889..5477
                     /vntifkey="33"
                     /label=Origin\of\replication
     repeat_region   complement(2966..3095)
                     /vntifkey="34"
                     /label=3'\ITR
     repeat_region   2..131
                     /vntifkey="34"
                     /label=5'\ITR
     polyA_signal    2751..2877
                     /vntifkey="25"
                     /label=Rabbit\globin\poly\A
     enhancer        328..427
                     /vntifkey="9"
                     /label=Alpha\mic/bik
     enhancer        222..321
                     /vntifkey="9"
                     /label=Alpha\mic/bik
     TATA_signal     886..889
                     /vntifkey="41"
                     /label=TATA
     promoter        443..902
                     /vntifkey="29"
                     /label=TBG
     CDS             1003..2655
                     /gene="luc+"
                     /codon_start=1
                     /product="luciferase"
                     /protein_id="AAA89084.1"
                     /db_xref="GI:1200463"
                     /vntifkey="4"
                     /label=luc+
     primer_bind     complement(1004..1026)
                     /vntifkey="28"
                     /label=Primer_Bind_1
BASE COUNT     1491 a      1438 c      1430 g      1556 t 
ORIGIN
        1 gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt 
       61 tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac 
      121 taggggttcc ttgtagttaa tgattaaccc gccatgctac ttatctacca gggtaatggg 
      181 gatcctctag aactatagct agaattcgcc cttaagctag caggttaatt tttaaaaagc 
      241 agtcaaaagt ccaagtggcc cttggcagca tttactctct ctgtttgctc tggttaataa 
      301 tctcaggagc acaaacattc cagatccagg ttaattttta aaaagcagtc aaaagtccaa 
      361 gtggcccttg gcagcattta ctctctctgt ttgctctggt taataatctc aggagcacaa 
      421 acattccaga tccggcgcgc cagggctgga agctaccttt gacatcattt cctctgcgaa 
      481 tgcatgtata atttctacag aacctattag aaaggatcac ccagcctctg cttttgtaca 
      541 actttccctt aaaaaactgc caattccact gctgtttggc ccaatagtga gaactttttc 
      601 ctgctgcctc ttggtgcttt tgcctatggc ccctattctg cctgctgaag acactcttgc 
      661 cagcatggac ttaaacccct ccagctctga caatcctctt tctcttttgt tttacatgaa 
      721 gggtctggca gccaaagcaa tcactcaaag ttcaaacctt atcatttttt gctttgttcc 
      781 tcttggcctt ggttttgtac atcagctttg aaaataccat cccagggtta atgctggggt 
      841 taatttataa ctaagagtgc tctagttttg caatacagga catgctataa aaatggaaag 
      901 atgttgcttt ctgagagaca gctttattgc ggtagtttat cacagttaaa ttgctaacgc 
      961 agtcagtgct tctgacacaa cagtctcgaa cttaagctgc acatggaaga cgccaaaaac 
     1021 ataaagaaag gcccggcgcc attctatccg ctggaagatg gaaccgctgg agagcaactg 
     1081 cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac agatgcacat 
     1141 atcgaggtgg acatcactta cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct 
     1201 atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga aaactctctt 
     1261 caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac 
     1321 gacatttata atgaacgtga attgctcaac agtatgggca tttcgcagcc taccgtggtg 
     1381 ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc 
     1441 caaaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc gatgtacacg 
     1501 ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtgcc agagtccttc 
     1561 gatagggaca agacaattgc actgatcatg aactcctctg gatctactgg tctgcctaaa 
     1621 ggtgtcgctc tgcctcatag aactgcctgc gtgagattct cgcatgccag agatcctatt 
     1681 tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt ccatcacggt 
     1741 tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt cttaatgtat 
     1801 agatttgaag aagagctgtt tctgaggagc cttcaggatt acaagattca aagtgcgctg 
     1861 ctggtgccaa ccctattctc cttcttcgcc aaaagcactc tgattgacaa atacgattta 
     1921 tctaatttac acgaaattgc ttctggtggc gctcccctct ctaaggaagt cggggaagcg 
     1981 gttgccaaga ggttccatct gccaggtatc aggcaaggat atgggctcac tgagactaca 
     2041 tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg taaagttgtt 
     2101 ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg cgttaatcaa 
     2161 agaggcgaac tgtgtgtgag aggtcctatg attatgtccg gttatgtaaa caatccggaa 
     2221 gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat agcttactgg 
     2281 gacgaagacg aacacttctt catcgttgac cgcctgaagt ctctgattaa gtacaaaggc 
     2341 tatcaggtgg ctcccgctga attggaatcc atcttgctcc aacaccccaa catcttcgac 
     2401 gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt 
     2461 ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta 
     2521 acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt 
     2581 accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa gaagggcgga 
     2641 aagatcgccg tgtaattcta gattccgaga tatcggtaat gggcccgcgt ggtacctcta 
     2701 gagtcgaccc gggcggcctc gaggacgggg tgaactacgc ctgaggatcc gatctttttc 
     2761 cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact tctggctaat 
     2821 aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa 
     2881 gcaattcgtt gatctgaatt tcgaccaccc ataataccca ttaccctggt agataagtag 
     2941 catggcgggt taatcattaa ctacaaggaa cccctagtga tggagttggc cactccctct 
     3001 ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt 
     3061 gcccgggcgg cctcagtgag cgagcgagcg cgcagcctta attaacctaa ttcactggcc 
     3121 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca 
     3181 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc 
     3241 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg 
     3301 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct 
     3361 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta 
     3421 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa 
     3481 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct 
     3541 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc 
     3601 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg 
     3661 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt 
     3721 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct 
     3781 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat 
     3841 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg 
     3901 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg 
     3961 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc 
     4021 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat 
     4081 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact 
     4141 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca 
     4201 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact 
     4261 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg 
     4321 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg 
     4381 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg 
     4441 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg 
     4501 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag 
     4561 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc 
     4621 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga 
     4681 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat 
     4741 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc 
     4801 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 
     4861 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 
     4921 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 
     4981 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc 
     5041 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 
     5101 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 
     5161 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 
     5221 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 
     5281 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 
     5341 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 
     5401 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 
     5461 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 
     5521 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta 
     5581 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag 
     5641 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga 
     5701 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg 
     5761 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg 
     5821 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc 
     5881 atgattacgc cagatttaat taaggcctta attag 
//
