LOCUS pWPT-CCC/ACC-mEG 10738 bp DNA circular 17-OCT-2013 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|676158593| COMMENT VNTDBDATE|676158593| COMMENT LSOWNER| COMMENT VNTNAME|pWPT-CCC/ACC-mEGFP-IRES-mCherry| COMMENT VNTAUTHORNAME|Josh| FEATURES Location/Qualifiers misc_feature 17..39 /vntifkey="21" /label=TIS:\\CCC/ACC CDS 34..762 /vntifkey="4" /label=mEGFP RBS 790..1365 /vntifkey="32" /label=IRES /note="Internal Ribosome Entry Site (IRES)" CDS 1393..2103 /vntifkey="4" /label=mCherry enhancer 2112..2710 /vntifkey="9" /label=WPRE primer_bind 10320..10437 /vntifkey="28" /label=cPPT /note="central PPT of HIV" splicing_signal 10198..10200 /vntifkey="38" /label=SA misc_feature 9602..9806 /vntifkey="21" /label=RRE misc_recomb 2848..2882 /vntifkey="86" /label=LoxP /note="LoxP" LTR 2728..3086 /vntifkey="19" /label=LTR/SIN /note="HIV LTR with U3 deletion" splicing_signal 9031..9034 /vntifkey="38" /label=SD /note="5' splice signal" misc_feature 8974..9111 /vntifkey="21" /label=psi /note="HIV packaging signal" LTR 8289..8922 /vntifkey="19" /label=LTR polyA_signal 7271..8120 /vntifkey="25" /label=pA /note="SV40 pA and intron" misc_feature 6412..6869 /vntifkey="21" /label=gpt promoter 5932..6187 /vntifkey="30" /label=SV40 /note="SV40 promoter/enhancer" misc_feature 5643..5643 /vntifkey="21" /label=ORI CDS 4018..4878 /vntifkey="4" /label=AmpR promoter 10470..10717 /vntifkey="29" /label=EF1-short BASE COUNT 2757 a 2680 c 2760 g 2541 t ORIGIN 1 tcgagtaact aactaaccca tgggttgatt accatgggcg aattcagcaa gggcgaggag 61 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag 121 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc 181 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac 241 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc 301 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac 361 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag 421 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac 481 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag 541 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc 601 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag 661 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc 721 gccgggatca ctctcggcat ggacgagctg tacaagttcg aataagcatg catctagggc 781 ggccaattcc gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa 841 taaggccggt gtgcgtttgt ctatatgtga ttttccacca tattgccgtc ttttggcaat 901 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct 961 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct 1021 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc 1081 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa 1141 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc 1201 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg 1261 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc 1321 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataagcttg ccacaacccg 1381 ggatccgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc 1441 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc 1501 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt 1561 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc 1621 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc 1681 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc 1741 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc 1801 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac 1861 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc 1921 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc 1981 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg 2041 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag 2101 taagcggccg ccgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt 2161 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat 2221 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct 2281 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct 2341 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc 2401 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg 2461 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc 2521 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac 2581 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg 2641 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc 2701 ccgcatcggg caattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag 2761 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa 2821 gacaagatgg gatcaattca ccatgggaat aacttcgtat agcatacatt atacgaagtt 2881 atgctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc 2941 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa 3001 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag 3061 tcagtgtgga aaatctctag cagcatctag aattaattcc gtgtattcta tagtgtcacc 3121 taaatcgtat gtgtatgata cataaggtta tgtattaatt gtagccgcgt tctaacgaca 3181 atatgtacaa gcctaattgt gtagcatctg gcttactgaa gcagacccta tcatctctct 3241 cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt ctgagtttct ggtaacgccg 3301 tcccgcaccc ggaaatggtc agcgaaccaa tcagcagggt catcgctagc cagatcctct 3361 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata 3421 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt 3481 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc 3541 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc 3601 taatgcagga gtcgcataag ggagagcgtc gaatggtgca ctctcagtac aatctgctct 3661 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg 3721 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg 3781 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc 3841 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt 3901 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 3961 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg 4021 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt 4081 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga 4141 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa 4201 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt 4261 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt 4321 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc 4381 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga 4441 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat 4501 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct 4561 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc 4621 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg 4681 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc 4741 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 4801 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca 4861 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta 4921 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 4981 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 5041 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 5101 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 5161 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc 5221 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 5281 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 5341 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 5401 cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt 5461 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 5521 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 5581 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 5641 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 5701 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat 5761 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 5821 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctgtgga 5881 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 5941 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 6001 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 6061 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 6121 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag 6181 gaggcttttt tggaggccta ggcttttgca aaaagcttgg acacaagaca ggcttgcgag 6241 atatgtttga gaataccact ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg 6301 gactcatgtg aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt 6361 ttcccctcga acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa 6421 aaatacatcg tcacctggga catgttgcag atccatgcac gtaaactcgc aagccgactg 6481 atgccttctg aacaatggaa aggcattatt gccgtaagcc gtggcggtct gtaccgggtg 6541 cgttactggc gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg 6601 atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct 6661 tcatcgttat tgatgacctg gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt 6721 atccaaaagc gcactttgtc accatcttcg caaaaccggc tggtcgtccg ctggttgatg 6781 actatgttgt tgatatcccg caagatacct ggattgaaca gccgtgggat atgggcgtcg 6841 tattcgtccc gccaatctcc ggtcgctaat cttttcaacg cctggcactg ccgggcgttg 6901 ttctttttaa cttcaggcgg gttacaatag tttccagtaa gtattctgga ggctgcatcc 6961 atgacacagg caaacctgag cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc 7021 tcgacgctag tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat 7081 ggtaaaacca tccctcactg gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc 7141 attgacccac gcgaaatcct cgacgtccag gcacgtattg tgatgagcga tgccgaacgt 7201 accgacgatg atttatacga tacggtgatt ggctaccgtg gcggcaactg gatttatgag 7261 tgggccccgg atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact 7321 acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa 7381 actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga 7441 gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag 7501 tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt 7561 agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag 7621 taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata 7681 caagaaaatt atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca 7741 taacatactg ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc 7801 tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat 7861 gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag gttttacttg 7921 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg 7981 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 8041 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 8101 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc 8161 ccaccccata ccctattacc actgccaatt acctagtggt ttcatttact ctaaacctgt 8221 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac 8281 ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg atctgtggat 8341 ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc caggggtcag 8401 atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga 8461 agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga 8521 tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt 8581 ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg 8641 actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc 8701 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc 8761 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa 8821 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga 8881 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga 8941 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc 9001 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg 9061 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc 9121 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat 9181 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc 9241 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga 9301 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat 9361 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac 9421 cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat gagggacaat 9481 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 9541 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 9601 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 9661 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 9721 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 9781 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 9841 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 9901 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 9961 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 10021 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 10081 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 10141 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 10201 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 10261 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact 10321 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata 10381 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttccg 10441 atcacgagac tagcctcgac aagcttgatc gatggctccg gtgcccgtca gtgggcagag 10501 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc 10561 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt 10621 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc 10681 aacgggtttg ccgccagaac acaggtgtcg tgacgcgcat ccaggcctaa gcttacgc //