Name                type                location 
------------------------------------------------------                      
M13 fwd             primer_bind         3..19               
T7                  promoter            18..42              
BsaI                misc_feature        44..49,57..62       
guide RNA           misc_RNA            64..143             
T3                  primer_bind         rev:155..174        
M13 rev             primer_bind         rev:196..212        
ori                 rep_origin          rev:379..967        
KanR                CDS                 rev:1138..1935      
bla                 promoter            rev:1936..2040