LOCUS       pEMS1164               18987 bp    DNA     circular     16-FEB-2010
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|546091127|
COMMENT     VNTDBDATE|546091127|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEMS1164|
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COMMENT                            (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 
COMMENT                            1.74233 0.555556 0 "18987 bp" "@L bp" 16 0 -0.871165 
COMMENT                            0 -10.8 3.19698 0.949103 Nil)) (CObjectList)) 
COMMENT               #136=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -108 100) 
COMMENT                     (CObjectList 
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COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "I-Sce I" "@N" 1 
COMMENT                                   -12.3821 -5.87638 0 -10 3.02214 1.17389 #138#) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "FRT (F5 mutant)" "@N" 1 11.863 12.8767 0 -10 
COMMENT                                   7.41799 1.17389 #140#) 
COMMENT                             #142=(CScratch 
COMMENT                                   (CWidget 0 (3 #7# 0) 1 2 0 0 #100# 30405512 
COMMENT                                    100) (LOGPEN 0 8 13395507) 10 1 5.67782 1.9 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Kozak consensus" "@N" 1 12.9566 11.0594 0 
COMMENT                                   -10 7.99245 1.17389 #142#) 
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COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #8# 0) 1 2 0 0 #96# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 5.43696 
COMMENT                                    5.67385 1 0.082322) 0.8 1.8 0) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "EGFP (mutated stop TAA ->TTA)" "@N" 1 
COMMENT                                   15.0747 8.41795 0 -10 14.7361 1.17389 #144#) 
COMMENT                             #146=(CWideArrow 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "Cre" "@N" 1 
COMMENT                                   11.3749 6.90041 0 -10 1.6984 1.17389 #146#) 
COMMENT                             #148=(CScratch 
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COMMENT                                   0.082322 1) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "FRT" "@N" 1 
COMMENT                                   13.4002 2.58551 0 -10 1.92318 1.17389 #148#) 
COMMENT                             #150=(CScratch 
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COMMENT                                   0.082322 1) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 early polyA signal" "@N" 1 17.6 
COMMENT                                   0.143841 0 -10 11.0645 1.17389 #150#) 
COMMENT                             #152=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #12# 0) 1 2 0 0 #96# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 5.69367 
COMMENT                                    6.23123 1 0.082322) 0.8 1.8 0) 
COMMENT                             #153=(CLabel (CWidget 0 (0 0) 1 2 0 0 #92# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "CAG" "@N" 1 
COMMENT                                   6.14274 13.7448 0 -10 2.14797 1.17389 #152#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #154=(CGroupWidget 
COMMENT                            (CWidget 22 (7 22 0) 1 2 0 0 Nil -178 100) 
COMMENT                            (CObjectList 
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COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #1# 0) 1 2 0 0 #108# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 1 
COMMENT                                    0.00198236 0.405723 1 0.082322) 0.8 1.8 1) 
COMMENT                             #156=(CLabel (CWidget 0 (0 0) 1 2 0 0 #92# 11 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "AmpR" "@N" 1 
COMMENT                                   -5.82957 13.1454 0 -10 2.87229 1.17389 #155#) 
COMMENT                             #157=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #2# 0) 1 2 0 0 #108# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 0.835356 
COMMENT                                    2.03622 2.50736 1 0.082322) 0.8 1.8 0) 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "AmpR" "@N" 1 
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COMMENT                            (CObjectList)) 
COMMENT                      #159=(CGroupWidget 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "5' homology sequence" "@N" 1 -16.4471 
COMMENT                                   2.84129 0 -10 10.4901 1.17389 #160#) 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "3' homology sequence" "@N" 1 -6.19991 
COMMENT                                   -12.1963 0 -10 10.4901 1.17389 #162#) 
COMMENT                             #164=(CWideArrow 
COMMENT                                   (CWideLine 
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COMMENT                                    5.0167 1 0.082322) 0.8 1.8 0) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "HPRT complementary sequence" "@N" 1 18.1375 
COMMENT                                   -4.50869 0 -10 14.9609 1.17389 #164#)) 
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COMMENT               #166=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -950 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #167=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -1229 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #168=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -671 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 1699618931 
COMMENT       (CStringList) 0 0 (CObList)))
FEATURES             Location/Qualifiers
     misc_recomb     12850..16661
                     /vntifkey="86"
                     /label=5'\homology\sequence
     misc_marker     complement(17760..18981)
                     /vntifkey="22"
                     /label=AmpR
     misc_marker     11399..12824
                     /vntifkey="22"
                     /label=AmpR
     misc_recomb     7758..11403
                     /vntifkey="86"
                     /label=3'\homology\sequence
                     /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3"
     misc_recomb     3804..7757
                     /vntifkey="86"
                     /label=HPRT\complementary\sequence
                     /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2"
     misc_feature    12831..12848
                     /vntifkey="21"
                     /label=I-Sce\I
     misc_feature    1769..1802
                     /vntifkey="21"
                     /label=FRT\(F5\mutant)
     misc_feature    1803..1814
                     /vntifkey="21"
                     /label=Kozak\consensus
     misc_feature    1815..2531
                     /vntifkey="21"
                     /label=EGFP\(mutated\stop\TAA\->TTA)
     misc_feature    2532..3557
                     /vntifkey="21"
                     /label=Cre
     misc_feature    3561..3594
                     /vntifkey="21"
                     /label=FRT
     misc_feature    3748..3798
                     /vntifkey="21"
                     /label=SV40\early\polyA\signal
     misc_feature    128..1754
                     /vntifkey="21"
                     /label=CAG
                     /note="1308"
BASE COUNT     4836 a      4379 c      4546 g      5226 t 
ORIGIN
        1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 
       61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 
      121 cgagctctgc aggcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 
      181 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 
      241 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 
      301 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 
      361 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 
      421 tcatcgctat taacatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc 
      481 ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg 
      541 gggcgggggg gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg 
      601 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt 
      661 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggga 
      721 gtcgctgcga cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc 
      781 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 
      841 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag 
      901 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt 
      961 gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg 
     1021 cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg 
     1081 cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg 
     1141 cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc 
     1201 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg 
     1261 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg 
     1321 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg 
     1381 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca 
     1441 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc 
     1501 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg 
     1561 ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg 
     1621 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac 
     1681 cggcggctct agacaattgt actaaccttc ttctctttcc tctcctgaca ggttggtgta 
     1741 cagtagcttc caccacgcgt ggcgcgccga agttcctatt cttcaaaagg tataggaact 
     1801 tcgccgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg 
     1861 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg 
     1921 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc 
     1981 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg 
     2041 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc 
     2101 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg 
     2161 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca 
     2221 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca 
     2281 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg 
     2341 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc 
     2401 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg 
     2461 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc 
     2521 tgtacaagtt atccaattta ctgaccgtac accaaaattt gcctgcatta ccggtcgatg 
     2581 caacgagtga tgaggttcgc aagaacctga tggacatgtt cagggatcgc caggcgtttt 
     2641 ctgagcatac ctggaaaatg cttctgtccg tttgccggtc gtgggcggca tggtgcaagt 
     2701 tgaataaccg gaaatggttt cccgcagaac ctgaagatgt tcgcgattat cttctatatc 
     2761 ttcaggcgcg cggtctggca gtaaaaacta tccagcaaca tttgggccag ctaaacatgc 
     2821 ttcatcgtcg gtccgggctg ccacgaccaa gtgacagcaa tgctgtttca ctggttatgc 
     2881 ggcggatccg aaaagaaaac gttgatgccg gtgaacgtgc aaaacaggct ctagcgttcg 
     2941 aacgcactga tttcgaccag gttcgttcac tcatggaaaa tagcgatcgc tgccaggata 
     3001 tacgtaatct ggcatttctg gggattgctt ataacaccct gttacgtata gccgaaattg 
     3061 ccaggatcag ggttaaagat atctcacgta ctgacggtgg gagaatgtta atccatattg 
     3121 gcagaacgaa aacgctggtt agcaccgcag gtgtagagaa ggcacttagc ctgggggtaa 
     3181 ctaaactggt cgagcgatgg atttccgtct ctggtgtagc tgatgatccg aataactacc 
     3241 tgttttgccg ggtcagaaaa aatggtgttg ccgcgccatc tgccaccagc cagctatcaa 
     3301 ctcgcgccct ggaagggatt tttgaagcaa ctcatcgatt gatttacggc gctaaggatg 
     3361 actctggtca gagatacctg gcctggtctg gacacagtgc ccgtgtcgga gccgcgcgag 
     3421 atatggcccg cgctggagtt tcaataccgg agatcatgca agctggtggc tggaccaatg 
     3481 taaatattgt catgaactat atccgtaacc tggatagtga aacaggggca atggtgcgcc 
     3541 tgctggaaga tggcgattaa gaagttccta ttctctagaa agtataggaa cttcagcggc 
     3601 cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa 
     3661 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact 
     3721 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 
     3781 aagcattttt ttcactgcga attctcgtaa aactcttcat ggcagtagtt attattctct 
     3841 ctctctctct ttttcttttt tcttgagaca ggatatttct ctgttgccca ggctggagtg 
     3901 cagtggcaca gtcttggctc actgcagcct ggacctcctg ggctcaagcc atcctcccac 
     3961 ctcagcctcc caagtagctg gggctacagg cacatggcca ccaggcccag ataatttttc 
     4021 atttttgtag agactgagtc tcaccatgtt acccaggttt attattctca ttttttagat 
     4081 gaagagactg aggtccagag aagctcaatg acttgcctag ttttacaaat ctcctgccat 
     4141 cacatacccc tcagcgtcct taataagagg gaggccacca actatgtgct gggcactgtg 
     4201 gtggatgctg gagctatagg gttgagtata taagaaatgg tgttgctgga gcaactgttg 
     4261 cttgcttacc tgacctatct gagaattaat tagcagggga acatattttt gttttcagat 
     4321 tcaatataag aacttgtgtg ggcaaaaata aagatcagta gtaataacag tagttcccat 
     4381 ttgctgactg tactgtccta agtgcatata tatatacata cacacacgca tacctatact 
     4441 cctctaatac tcaaaatgat cctgtttatg tattgttaat atgctcattt tatttttaaa 
     4501 tttttattta tttttatttt tatttatttt tgagacggag tctcattctg tcgcggaggc 
     4561 tgaagtgcag tggtgcgatc tcagctcagt gcgacctccg cctcccgggt tcaagtgatt 
     4621 ctcctgcctc agcctccgga ctagctggga ttacaggcgc ccgcctccac gcccagctaa 
     4681 tttttgtatt tttagtagag atggggtttc gccatgttgg ccaggctggt ctcgtactcc 
     4741 tgaccttgag tgatccacct gcctcggcct cccaaagtgc tgggattaca ggcatgagcc 
     4801 accgcgccgg gctaatatgc tcattttagt gaggcaaaaa tagaggctca gagtctgatt 
     4861 tgtacaaaac tacagagcag ttaagtgtcc tctcagatgt gtaccctgat ctgggtgact 
     4921 ctaggactct aggtctcaac tgttacaacc agttaagggt ttggggaagc actgggccaa 
     4981 gagtcaggaa aatggaagcc acaggtagtg caaggtcttg ggaatgggac gtctggtcca 
     5041 aggattcacg cgatgactgg aacccgaaga gccggggccc ggtttacggc cgccatgaag 
     5101 caacgcgcgc cggtaggttt gggaatcagg gagccctctg aataggagac tgagttggga 
     5161 gggaaagggg cttcgctggg ggagcctcgg cttcttctgg gagaaaattc ccacggctac 
     5221 ctagtgagcc tgcaaactgg taggcgccgg cgtaggcgcg cgggcggggc cgggggcggg 
     5281 gcctgcgggg cgtggcgggg cgggcagagg gcggggcctg cttctcctca gcttcaggcg 
     5341 gctgcgacga gccctcaggc gaacctctcg gctttcccgc gcggcgccgc ctcttgctgc 
     5401 gcctccgcct cctcctctgc tccgccaccg gcttcctcct cctgagcagt cagcccgcgc 
     5461 gccggccggc tccgttatgg cgacccgcag ccctggcgtc gtggtgagca gctcggcctg 
     5521 ccggccctgg ccggttcagg cccacgcggc aggtggcggc cgggccctga ggcgcgggat 
     5581 cgatccatga cacccccccg ccccccatat ctcactataa ttcactgtca aatggctttt 
     5641 atgatttgga gtttggggat acttttaaag acagggtctt ggtagctcct agatggcttg 
     5701 ggattcactt agtaaccctt gctggcctaa aattcaggaa cttctacctc atcttcccaa 
     5761 gtactgggtt agtagggatg tccagcatat ttagcttgct ttaatgattt tgctgtactt 
     5821 ttttctgtgt gtacaggtgc atgtatattt ataaaactaa cataatatat tttaccccca 
     5881 aagtaccata accctaatta atgctattaa atgttttgta tgctgggctc tgggcatact 
     5941 aatataacca atattataca atgcaaaaca attttggaat atcttctgta aaattatctt 
     6001 attagtaagt tgtaaccatc tcagacttct aagtttgagt ttgtaaagga tcagtttttt 
     6061 ttggtgacct acacacacac acacacacac acacacacac acacacacag gggggtggat 
     6121 tctgagaatt tagagaattt agatggttaa taccagtttt tatttttatt ctgtgtgata 
     6181 tttatttatt aagatttatt tatttattac atgtaggtac actgtatctg tcttcagaca 
     6241 caccagaaga gggcatcaga tctcgttaca gatggttgtg agtcaccatg tggttgctgg 
     6301 gatttgaact caggacctct ggaagaacag tcagtgctct taaccgttga gccatctcac 
     6361 cagccccgtg atattttatt taacttacta gaatatactg tgttaaaaca tattttaggg 
     6421 gattggggag atggcccatt ctgtaaagtg tctgccatgt catcttcagg acctgcgttt 
     6481 gtaaccccag catggagaag acagagacag ggagacccct gggcttcctg gcaagctaga 
     6541 ctagcaaatt tggttaacta ggttcaatga gagactcagt ctcaaaacat aagaagtgaa 
     6601 ttttttagac ccaatttcaa tctctgaact ccatacgtat gcacatgtac ctgcatataa 
     6661 atatatatac acacccataa acacatgcac acacaaaaaa tggttcaaaa gttcttaaaa 
     6721 tgtataagag tagaaagtgt agagccggga gtggtggcgc acgcctttaa tcccagcact 
     6781 tgggagcaga ggcaggtgga tttctgagtt cgaggccagc ctggtctaca gagtgagttc 
     6841 caggacagcc agagctatac agagaaatcc tgcctcaaaa aaccaaaaat aaagtgtata 
     6901 tactaatctt tcatgcctaa aatatttatt tttgccacat ttttcatacc tctttaatat 
     6961 ttttcttttt ctctttgtgc ttcaaaggaa attttgtgtc atgttttact tttcattctt 
     7021 gactctattc agcagtaaga cgcagcatgg ctttcttaga gaagtataat ctattacaaa 
     7081 acggtaacat ttaactgctc tacataccct cttgtagcta aattcctctg ttaaactaag 
     7141 acttattatc taaacgtaat taaatgttga tatcttataa tgctccactt tgaaacagct 
     7201 gtctttcttt tgcagattag cgatgatgaa ccaggttatg acctagattt gttttgtata 
     7261 cctaatcatt atgccgagga tttggaaaaa gtgtttattc ctcatggact gattatggac 
     7321 aggttagtaa gacctcgatt gaagttattt ttagagggtg tgtgtgtggt gtgtgtgtgt 
     7381 gtgtgtgtgt gtgtgtgtgt gtgtgtgtaa gagagagagg agaggcatgt gtgatatata 
     7441 tgtgaagtgt attgcatgta catggaatcc agagaaggac attaggtgtc ctactctgtt 
     7501 gctctcttat tcccttgaat cagtgtctct tgctgaacct ggggcataca tactgatttg 
     7561 gctaggctgc ctggctagca agacccaggg atctgcttgt ctctgccccc cagtgctgac 
     7621 gttacaagca ggtgcaacca catctggttt ttatatgggt actgggggat ctgaacttag 
     7681 ggcctgatgc gtgcccaagt gctctcaccc gccaagccac ctctccagcc aaagggtttt 
     7741 ttttcttgta ttaattctaa cttagcaact gttgagtagt tgatgtttga atataagcta 
     7801 agccaactta ttagaataaa tgttttctat tttttacttg tatgtgtgag tgcctgcatg 
     7861 tatgtatgtg aagcatatgt attccaggat gtcagaattg tgatggattc cctggaactg 
     7921 gagttacaga ccattgtgaa ccactgtgta tgggtgctgg gaactgaact ttaggtcctc 
     7981 tgcaaaaaca gtaaggatct taactgataa actatctttc tagccctaag gctttctact 
     8041 tttaaagttc gtttctcttt ctctctctct ctctctctct ctctctctct ctccctctcc 
     8101 ctccctcctc ctcctcctcc tctttccccc ctctcgcctc ctctttctct ctcccctccc 
     8161 tctctctctc tcacacacac acacagacag agagaggaga gagaaaggaa gccttactaa 
     8221 ttaattacct agttttagac ataactagtg ggaatccata gtaagtaaat caacattctt 
     8281 gaagagaggt tagaagaatt ggctctcgtt gtccatcgag gctgtataag attattcttc 
     8341 tgttttctca gcttaccata catattttct acgtagacaa taccaattca aatacactgt 
     8401 acgtaaagta tatgcgcaca cacacacaca cacacacaca cacacacaca caagttacat 
     8461 attgtcatat agtactaaaa tttatggact ttttcatatt aatacatcta gagctatcat 
     8521 gttttttatc ttttaaaaat tactttacgg ggctacagag atgtctcagc agttaggagc 
     8581 actgactgct cttccagagg tcctgagttc aattcccagc aaccacatgg tggctcacaa 
     8641 ccatctgtaa tgggatctga tccactctta tggtgtgtct gaagacagtg acagtgtact 
     8701 catatacatg aaataaatct ttaaaaaatt actttaccat cagggtatat atatatatat 
     8761 atatgtgtgt gtgtgtgtgt gtgtgtatga agtatttgct atttgagagg gtctcactgt 
     8821 ttagctcagt ctggcttgga acgttctgta tagcccagac tggcctcaac tcagcagtcc 
     8881 ccctgaaagg ctctcatatg tggagattac aggtatgtgc tactgtgccc agccctacat 
     8941 gttactttgt ttttgaaata gggtatcaaa tagcttagac tggcttctaa gtcagtgtgt 
     9001 agctaaggat ggtctgagct tctgaacctg ctacttcaac tcctggtgtg ccatcatggc 
     9061 cagtttgcac agttgtgaga gtagaactca ggacctccta tgtgttacta gacatgttct 
     9121 ctaccagctg agctacatcc caattcgcct acatttactt ttatttttta aaagatttat 
     9181 ttatgtatac agcactctgc ctgcatgtac atctgcatgc cagaagaggg caccagatct 
     9241 catcatacat agttgtgagc caccatgtgg ttgctgggga ttgaactcag ggcctctgga 
     9301 agagcagtca gtgctcttaa cctctgagcc atctctgtag ccccctacat ttattttaaa 
     9361 gattataggc tattctattc atagatgtgc cataactgat ttaaccagat cttcattatt 
     9421 ttgtacttag tagagtatat tagagggaat gaatcttaaa tacacatttt gacacttgct 
     9481 cagctttaga caagtaaaaa tcactggtca aggaaatggt gcttagtgga tgcttctgaa 
     9541 ctgtcaaatt gtcctccaac aagttacatc attttatact tccaacgtta tgtgagagtt 
     9601 catttttctt taggctcttc tagtatttta tatttgagaa ataaaattta accaaaattt 
     9661 gccaatctag taagcttgtt tttatttgta tctttaaatt ttatgataga tgttatagtg 
     9721 tactctcctc tcccctcccc tcccctctcc tcctcagcag gatcttgtta tgtagcccta 
     9781 agggatctaa tacttactct gtagtccagg ctgtccttca gctcatgaga cacaccatca 
     9841 tgcttggcat gtttcttgtt ctgaaaggta tctggtttta attttggttg agctggtgtg 
     9901 ttggtgtata tctgtaatct cagcacaggc aagtagagac aaggattatg attacagtcc 
     9961 agcctgggct atttagaggg attctcttta atctttcctc atgccccaaa atcttacctt 
    10021 tggtatatga aaaatagtct ccacttctgc aaaatattgc tttatgaagt aagaattaat 
    10081 tcccttcata gagacaagga atgtgtcctg taaaagttta atgtgtaaga agtatttgtt 
    10141 ataaaagata aatattcaga atcttctttt taattcctga ttttatttct ataggactga 
    10201 aagacttgct cgagatgtca tgaaggagat gggaggccat cacattgtgg ccctctgtgt 
    10261 gctcaagggg ggctataagt tctttgctga cctgctggat tacattaaag cactgaatag 
    10321 aaatagtgat agatccattc ctatgactgt agattttatc agactgaaga gctactgtgt 
    10381 aagtataatt aacttataat taaaaaaata gggccattct agttttatct atattttttt 
    10441 taaacttgtg caaactatgc tacataatca acttacttga ttatgtaata tatatagtaa 
    10501 tagttggaat tactattatt acatatttat ttttctgtgc tggggtagaa cccagggaac 
    10561 taagtactgt aataaccgag cttcattcat cctagtcatt tttaattttt tttttttttt 
    10621 ttgttttttc gagacaaggt ttctctgtgt agccctggct gtcctggaac tcactctgta 
    10681 gaccaggctg gcctcgaact cagaaatctg cctgcctctg cctcccagtg ctgagattaa 
    10741 tgacgtgcac caccactgcc tggcattttt ttaaagtttt aatttgagac cctttctcac 
    10801 taacttgctc acactatcct cgaactcatt acgtgggctg gtctagaaat ttcagtcaac 
    10861 ttgcctcagc ttctcaagta gctgggatta caggcaggtg ctaccacttc tggccatgat 
    10921 cattgttgtt gttgctatta ttattattat tatttcactg atacttcata tcacaacaca 
    10981 gaatagtaac tgtagttcat gaccaacaaa aaagttgtac agtttctctg ttgtcttatt 
    11041 ttgatctgga ttttgatttg tttcatggca ctgggcaggc taatctcaaa ctgcatggct 
    11101 caaatagtcc ttctgtctta atctcccatg ttctggaagt ataggtatca ctacacctag 
    11161 ctgtattttc atattttaaa aaaaacgcct aagaattgct attgaatgag gcggagagat 
    11221 ggctcagcag tcacaagtac ttgtagagga ctggggtttg gttactagca cctcaaagat 
    11281 ggcttacaat tatctgtagc tccaattcca gaggatctgt gtggtaaagc tgcatgtata 
    11341 tgggcatgca ggtaaaactg ctaaacatat aaaaatagct cagtcttaag agaattggtc 
    11401 gactttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 
    11461 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga 
    11521 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca 
    11581 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat 
    11641 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata 
    11701 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 
    11761 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 
    11821 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 
    11881 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 
    11941 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg 
    12001 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 
    12061 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 
    12121 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 
    12181 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 
    12241 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 
    12301 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 
    12361 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 
    12421 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga 
    12481 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 
    12541 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 
    12601 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 
    12661 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 
    12721 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 
    12781 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccaggtcgag tagggataac 
    12841 agggtaatgg atcctcgttg ggcacctgca ctcatgtacg caggcccaca tatagatgta 
    12901 cacacgcata tcattcaaaa tagaaattat ttatacaagt agtgcgttgg agagctggct 
    12961 caggagttgt taatgcttgc tacccttgca aaggacccaa gttcaatttc cagcacctgt 
    13021 gttgggcatc tcacaaccac atgtaaatct agtttcaggg gatttcaccc ctcttctgaa 
    13081 ctcttcaggc acttataccc acaaatggac atctccacat agagacacat acttttaaaa 
    13141 aagtaatcta gggtctagag aaatggttat agaccacgga ctgggatcag ttcccagaag 
    13201 ccacatcctg tggctcacaa ttgcctgtga atcaagttct agatctggtg gcctcgtcta 
    13261 gcctgcagtc atcaacaaac acatgcacac atccatacac acataattta aaagaaagcg 
    13321 aaagtaacct ataatgctaa acaaagcaca ttctcagtgt agactgcagt ggtgatgaac 
    13381 cttgaaaata tattaataga agccagacac gtaagtcaca aatggtataa ctcaatttat 
    13441 atgaaattcc cagagagtcc aaagttaaaa aaaacaaaaa ggcagattat tggttgtcag 
    13501 tagctacaga gatgggacat tgaagatatt aataggtata gggttgagga ttcatggcct 
    13561 ttccctcttc catgttagca tgtctattgt tgttaacctt attcaactca tgttagggca 
    13621 atcatattga tgagacttaa tgagtatagc ttgacattcc tagaagacag agtctcacat 
    13681 cagactctct gatcctctga ctcttagaat ctttcaacac cctcttctga aatgatctct 
    13741 gagcctttgg ttgtgactga attatgagaa agaattttat gttgaatgaa ttttacttca 
    13801 gcatttttac aaaactgaag ctggggatgg cgtttcatgc gtgcagtccc taccatttgc 
    13861 ctataagcct ggaggaggca gaagtagaat taggcttttc agggacatct tcagctgcat 
    13921 catgagcttg aggacaactt gagctacata agattcccat ccccaccccc acacacacac 
    13981 acacatctag tatgtaccta gtatgcagtc ttttcatatg tttaaaatgt agaaaggtat 
    14041 atgtgaagtc tcccttttcc ccacaactta gtccctccaa agtattgcat cagtttcctt 
    14101 gtacatgatt ttatgcatat ataagataca ctatttctct ctctctctct ctccttccct 
    14161 ccttccctcc aacctctggg tatgagttga gggttatgta tgtttcaagt tgtaataaga 
    14221 attgccactc aaacatttca tcccatttta agttaagttt gtctgatttt ccactttaga 
    14281 agggcagcca tgatggctca tgcctataat cccatcctag cacttgtaag cctaatacat 
    14341 aagactggag gattgtcatc agcttgaggc tagcctggtc tacctaatga gttccagcca 
    14401 gccaagagta gaatataaga ccctgtctcc aaaaaatgta ccaaaggggc tggaggggta 
    14461 actcagtggt taagaacagt agctgctctt ccagaggacc tgggttcaat tcccagcacc 
    14521 cacatggcag cttgcaacta tctgtaactg tccagctttg ggatatccga caccattata 
    14581 ccaattacat aaaatgaaaa aattgtaaaa tgtgctaaaa tgagtgggga gggggagcat 
    14641 ggtgtggttc tgtgggcttg ggattttgcc atgaatccat gactatatct cagcaatatg 
    14701 tggattattt ctggaaacct gtggggcatg ggtgtatggg aagaggggag agagagagag 
    14761 cccgcatcca gccagagttc ctgtgctctg ggcaggcgga cgtgggagga ctgctggacg 
    14821 atttccactt ggccctgggt gggcatctgg ctgtgtgaag ccactgaccc cactcagcgg 
    14881 ggggtagata aggggcattt cccataccag gttctcagac tccagcattc cacgctggat 
    14941 acagcagaag agctgagaac agaatagggg ccccagggag acactctcgt cccagagata 
    15001 aagaaggcag agggagagag attcctacgc aggcgagagg ccttagtctg ggccttgatg 
    15061 aacagagacg gtctatggtt ttagagcttt attgtagaaa ggcaggggga aagagagaag 
    15121 gtagaaagag agagaccagc catggccaag aggagagaag gggggaaagg agagagagaa 
    15181 gaaaggctag agagtaagaa aggttaaaga gacagaagta aaagcttaga gtaagagaga 
    15241 gagagagagc aagagggaga gtaagaaaag taaaagctta agagagcgag gaggggccag 
    15301 cccctctaat agtgggcttg ttatcttgtt gttgctaggt aactgtgaag cgtctagcct 
    15361 gaaggccaga agcttgggac attgtctatg tgactgaaag ccatgagcct ctactgtggg 
    15421 ggctgtggag gtggtaactt ggacaggagc caggggttcc aggagacatg agggaatgcc 
    15481 ttctgtccca tgtaggtagg aattaccacc accggctttc accgtagttc actggagact 
    15541 gtttgtacat agcccattgc cccacagaaa ccaggcactc actgtttcat ttgcataaga 
    15601 attagaaggc tttattttgg ctgggattgg tgacttgccc cagaaattcc agcttttggg 
    15661 aagccgaatc ctggagaacc aggagttcaa ggtcatcctc aggtacctca tttgttcaag 
    15721 accagcctgt gctaccatga gaccttgtcc ctactcccct caaccccaaa acacgctgtt 
    15781 ttcattttat aggttgtact aaccatggag gatggtcatt tgcctttctc ttgatttcag 
    15841 aagcgtagcg cgggctttgc accatgatct agtgtttcca gataggcttg cctctccaca 
    15901 gccaggctta tcctgcttca gtcccatgtt tggcaagggc aacatatgac tgaagaactt 
    15961 gcatagcctt atggtgtatc atgactctta gccccagttt ataactgtct aacagctggt 
    16021 ggcttttgtg aaaaaggaaa gcatcatttg aacttttaac ttttcagctg tttgttttga 
    16081 ctttgcataa atactgagac tagctaggaa atgagctttt gtttcatttg ctaaaaatga 
    16141 tttttttttc atgaggcaat ttcaagcaat ttgaatttag caatctctac ttgtctgaaa 
    16201 agaagttatt aattaaaaaa aaacaaacaa acaggaacct attatgctgg ctagtcacat 
    16261 gaaagaatcc aaatgaacac agtcaaggct gaatcaacac atgaaattat tttctaattc 
    16321 cccaaattac atttatgcta attagtatgt agtaaaacat tttaattgtt tccaatgtta 
    16381 aacattacag aaataatttt gatgttttcc taataaccca gcctttgatc atgtttgcat 
    16441 ttatttgatt gttgtcagtt tattaaataa ttaagtaaga gcaacaagat aaaatgactt 
    16501 catgagagtc cttttctgta acttagaacg tcagtagtca taggaactgc ggtcgttccc 
    16561 ttacctttca gaagtaggct aaagagttga acgcaaaggt ggtttttcga atcttcctga 
    16621 tccttaagcc cccactaggt ttatattcta tatttgatat cggattcgaa cccctcgagc 
    16681 ttaggatccg gaacccttaa tataactaag ttattaggtc cctcgaagag gttcactagt 
    16741 actggccatt gcggccgctt cgaggctgcc tcgcgcgttt cggtgatgac ggtgaaaacc 
    16801 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca 
    16861 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc 
    16921 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt 
    16981 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 
    17041 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 
    17101 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 
    17161 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 
    17221 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 
    17281 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 
    17341 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 
    17401 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 
    17461 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 
    17521 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 
    17581 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 
    17641 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 
    17701 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 
    17761 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 
    17821 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 
    17881 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 
    17941 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 
    18001 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 
    18061 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 
    18121 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 
    18181 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 
    18241 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 
    18301 cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 
    18361 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 
    18421 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 
    18481 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 
    18541 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 
    18601 ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 
    18661 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 
    18721 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 
    18781 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 
    18841 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 
    18901 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 
    18961 atttccccga aaagtgccac ctgacgg 
//
