LOCUS       pCAG-roxSTOProx-ZsGreen      6430 bp    DNA     circular 
FEATURES             Location/Qualifiers
     Promoter        9..1676
                     /label=CAG Promoter
     Promoter        1686..1708
                     /label=T7
     SSR_recognition 1724..1755
                     /label=rox
     misc            1756..2592
                     /label=3polyA-STOP
     SSR_recognition 2593..2624
                     /label=rox
     CDS             2638..3332
                     /label=ZsGreen
     PolyA_signal    3340..3610
                     /label=BGHpA
     CDS             4398..5258
                     /label=Amp
ORIGIN
        1 CCGGATTCGA CATTGATTAT TGACTAGTTA TTAATAGTAA TCAATTACGG GGTCATTAGT
       61 TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC CGCCTGGCTG
      121 ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA TAGTAACGCC
      181 AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG CCCACTTGGC
      241 AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG ACGGTAAATG
      301 GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT GGCAGTACAT
      361 CTACGTATTA GTCATCGCTA TTACCATGGT CGAGGTGAGC CCCACGTTCT GCTTCACTCT
      421 CCCCATCTCC CCCCCCTCCC CACCCCCAAT TTTGTATTTA TTTATTTTTT AATTATTTTG
      481 TGCAGCGATG GGGGCGGGGG GGGGGGGGGG GCGCGCGCCA GGCGGGGCGG GGCGGGGCGA
      541 GGGGCGGGGC GGGGCGAGGC GGAGAGGTGC GGCGGCAGCC AATCAGAGCG GCGCGCTCCG
      601 AAAGTTTCCT TTTATGGCGA GGCGGCGGCG GCGGCGGCCC TATAAAAAGC GAAGCGCGCG
      661 GCGGGCGGGA GTCGCTGCGT TGCCTTCGCC CCGTGCCCCG CTCCGCGCCG CCTCGCGCCG
      721 CCCGCCCCGG CTCTGACTGA CCGCGTTACT CCCACAGGTG AGCGGGCGGG ACGGCCCTTC
      781 TCCTCCGGGC TGTAATTAGC GCTTGGTTTA ATGACGGCTC GTTTCTTTTC TGTGGCTGCG
      841 TGAAAGCCTT AAAGGGCTCC GGGAGGGCCC TTTGTGCGGG GGGGAGCGGC TCGGGGGGTG
      901 CGTGCGTGTG TGTGTGCGTG GGGAGCGCCG CGTGCGGCCC GCGCTGCCCG GCGGCTGTGA
      961 GCGCTGCGGG CGCGGCGCGG GGCTTTGTGC GCTCCGCGTG TGCGCGAGGG GAGCGCGGCC
     1021 GGGGGCGGTG CCCCGCGGTG CGGGGGGGCT GCGAGGGGAA CAAAGGCTGC GTGCGGGGTG
     1081 TGTGCGTGGG GGGGTGAGCA GGGGGTGTGG GCGCGGCGGT CGGGCTGTAA CCCCCCCCTG
     1141 CACCCCCCTC CCCGAGTTGC TGAGCACGGC CCGGCTTCGG GTGCGGGGCT CCGTGCGGGG
     1201 CGTGGCGCGG GGCTCGCCGT GCCGGGCGGG GGGTGGCGGC AGGTGGGGGT GCCGGGCGGG
     1261 GCGGGGCCGC CTCGGGCCGG GGAGGGCTCG GGGGAGGGGC GCGGCGGCCC CGGAGCGCCG
     1321 GCGGCTGTCG AGGCGCGGCG AGCCGCAGCC ATTGCCTTTT ATGGTAATCG TGCGAGAGGG
     1381 CGCAGGGACT TCCTTTGTCC CAAATCTGGC GGAGCCGAAA TCTGGGAGGC GCCGCCGCAC
     1441 CCCCTCTAGC GGGCGCGGGC GAAGCGGTGC GGCGCCGGCA GGAAGGAAAT GGGCGGGGAG
     1501 GGCCTTCGTG CGTCGCCGCG CCGCCGTCCC CTTCTCCATC TCCAGCCTCG GGGCTGCCGC
     1561 AGGGGGACGG CTGCCTTCGG GGGGGACGGG GCAGGGCGGG GTTCGGCTTC TGGCGTGTGA
     1621 CCGGCGGCTC TAGAGCCTCT GCTAACCATG TTCATGCCTT CTTCTTTTTC CTACAGATCC
     1681 TTAATTAATA ATACGACTCA CTATAGGGGG TCGACCCGCC ACCTAACTTT AAATAATTGG
     1741 CATTATTTAA AGTTATCGCG ATGAATAAAT GAAAGCTTGC AGATCTGCGA CTCTAGAGGA
     1801 TCTGCGACTC TAGAGGATCA TAATCAGCCA TACCACATTT GTAGAGGTTT TACTTGCTTT
     1861 AAAAAACCTC CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT
     1921 TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC
     1981 AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC
     2041 TTATCATGTC TGGATCTGCG ACTCTAGAGG ATCATAATCA GCCATACCAC ATTTGTAGAG
     2101 GTTTTACTTG CTTTAAAAAA CCTCCCACAC CTCCCCCTGA ACCTGAAACA TAAAATGAAT
     2161 GCAATTGTTG TTGTTAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC
     2221 ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA
     2281 CTCATCAATG TATCTTATCA TGTCTGGATC TGCGACTCTA GAGGATCATA ATCAGCCATA
     2341 CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC CTGAACCTGA
     2401 AACATAAAAT GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA
     2461 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT
     2521 GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG GATCCCCATC AAGCTGATCC
     2581 GGAACCCTTA ATTAACTTTA AATAATTGGC ATTATTTAAA GTTAGGCAGC CGCCACCATG
     2641 GCCCAGTCCA AGCACGGCCT GACCAAGGAG ATGACCATGA AGTACCGCAT GGAGGGCTGC
     2701 GTGGACGGCC ACAAGTTCGT GATCACCGGC GAGGGCATCG GCTACCCCTT CAAGGGCAAG
     2761 CAGGCCATCA ACCTGTGCGT GGTGGAGGGC GGCCCCTTGC CCTTCGCCGA GGACATCTTG
     2821 TCCGCCGCCT TCATGTACGG CAACCGCGTG TTCACCGAGT ACCCCCAGGA CATCGTCGAC
     2881 TACTTCAAGA ACTCCTGCCC CGCCGGCTAC ACCTGGGACC GCTCCTTCCT GTTCGAGGAC
     2941 GGCGCCGTGT GCATCTGCAA CGCCGACATC ACCGTGAGCG TGGAGGAGAA CTGCATGTAC
     3001 CACGAGTCCA AGTTCTACGG CGTGAACTTC CCCGCCGACG GCCCCGTGAT GAAGAAGATG
     3061 ACCGACAACT GGGAGCCCTC CTGCGAGAAG ATCATCCCCG TGCCCAAGCA GGGCATCTTG
     3121 AAGGGCGACG TGAGCATGTA CCTGCTGCTG AAGGACGGTG GCCGCTTGCG CTGCCAGTTC
     3181 GACACCGTGT ACAAGGCCAA GTCCGTGCCC CGCAAGATGC CCGACTGGCA CTTCATCCAG
     3241 CACAAGCTGA CCCGCGAGGA CCGCAGCGAC GCCAAGAACC AGAAGTGGCA CCTGACCGAG
     3301 CACGCCATCG CCTCCGGCTC CGCCTTGCCC TGaaccGAGC TCGCTGATCA GCCTCGACTG
     3361 TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG
     3421 AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA
     3481 GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG
     3541 AAGACAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG GCGGAAAGAA
     3601 CCAGCTGGGG CTCGAGATCC ACTAGTTCTA GCCTCGAGGC TAGAGCGGCC GCCACTGGCC
     3661 GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA
     3721 GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC
     3781 CAACAGTTGC GCAGCCTGAA TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG
     3841 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT
     3901 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA
     3961 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA
     4021 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT
     4081 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC
     4141 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG
     4201 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGCTT
     4261 ACAATTTAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT
     4321 AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT
     4381 ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG
     4441 CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG
     4501 AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC
     4561 TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT
     4621 GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT
     4681 ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA
     4741 TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT
     4801 TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG
     4861 ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG
     4921 AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG
     4981 AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG
     5041 CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG
     5101 CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC
     5161 GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA
     5221 TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT
     5281 ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC
     5341 TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG
     5401 ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT
     5461 GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC
     5521 CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC
     5581 TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG
     5641 CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT
     5701 TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT
     5761 GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC
     5821 TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA
     5881 GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA
     5941 GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG
     6001 GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT
     6061 GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA
     6121 CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG
     6181 TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA
     6241 TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG
     6301 CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG
     6361 CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC
     6421 ATGAGGCGCG
//
