LOCUS Exported File 7118 bp ds-DNA circular SYN 10-FEB-2014 DEFINITION . ACCESSION . VERSION . KEYWORDS AAV2_hSyn_oChIEF_tdTomato SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7118) AUTHORS . TITLE Direct Submission JOURNAL Exported Feb 10, 2014 from SnapGene Viewer 1.1.2 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7118 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 378..509 /label="5'ITR" /note="5'ITR" /note="color: #00ffff" misc_feature 570..1041 /label="hSyn" /note="hSyn" /note="color: #a3ff98" misc_feature 1059..1064 /label="BamHI" /note="BamHI" /note="color: #ff7ec1" misc_feature 1066..1074 /label="Kozak sequence" /note="Kozak sequence" /note="color: #00ffff" misc_feature 1069..2145 /label="oChIEF" /note="oChIEF" /note="color: #ff9da9" misc_feature 2146..2151 /label="WPRE(1)" /note="WPRE(1)" /note="color: #47ff46" misc_feature 2152..3582 /label="tdTomato" /note="tdTomato" /note="color: #fffb30" misc_feature 2152..2829 /label="Tomato copy 1" /note="Tomato copy 1" /note="color: #ff0600" misc_feature 2899..3555 /label="Tomato copy 2" /note="Tomato copy 2" /note="color: #ff5c1c" misc_feature 3583..3589 /label="NheI" /note="NheI" /note="color: #00ffff" misc_feature 3592..3597 /label="EcoRI" /note="EcoRI" /note="color: #00ffff" misc_feature 3604..3609 /label="HindIII" /note="HindIII" /note="color: #00ffff" misc_feature join(3610..4073,4085..4225) /label="WPRE" /note="WPRE" /note="This feature has 2 segments: 1: 3610 .. 4073 / #47ff46 2: 4085 .. 4225 / #47ff46" misc_feature 4074..4084 /label="f1 origin" /note="f1 origin" /note="color: #ff8000" misc_feature 4264..4476 /label="SV40 polyA" /note="SV40 polyA" /note="color: #996633" misc_feature 4547..4677 /label="3' ITR" /note="3' ITR" /note="color: #00ffff" misc_feature 4859..5176 /label="f1 origin(1)" /note="f1 origin(1)" /note="color: #ff8000" misc_feature 5440..6299 /label="Amp" /note="Amp" /note="color: #00ffff" ORIGIN 1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct 61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa 121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg 181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag 241 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga 301 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccagattt 361 aattaaggcc ttaattaggc tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc 421 cgggcgtcgg gcgacctttg gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg 481 agtggccaac tccatcacta ggggttcctt gtagttaatg attaacccgc catgctactt 541 atctacgtag ccatgctcta ggaagatctc tgcagagggc cctgcgtatg agtgcaagtg 601 ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga ccccacccac 661 tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag agggggaggg 721 gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg acagtgcctt 781 cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg ctgacgtcac 841 tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc gccgccgccg 901 gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc gaccatctgc 961 gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga ggagtcgtgt 1021 cgtgcctgag agcgcagtcg aattcaagct gctagcaagg atccgaccat ggtgagcaga 1081 agaccctggc tgctggccct ggccctggcc gtggccctgg ccgccggcag cgccggcgcc 1141 agcaccggca gcgacgccac cgtgcccgtg gccacccagg acggccccga ctacgtgttc 1201 cacagagccc acgagagaat gctgttccag accagctaca ccctggagaa caacggcagc 1261 gtgatctgca tccccaacaa cggccagtgc ttctgcctgg cctggctgaa gagtaacggc 1321 accaacgccg agaagctggc cgccaacatc ctgcagtgga tcaccttcgc cctgagcgcc 1381 ctgtgcctga tgttctacgg ctaccagacc tggaagagta cctgcggctg ggaggagatc 1441 tacgtggcca ccatcgagat gatcaagttc atcatagagt acttccacga gttcgacgag 1501 cccgccgtga tctacagcag caacggcaac aagaccgtgt ggctgagata cgccgagtgg 1561 ctgctgacct gccccgtggt cctgatccac ctgagcaacc tgaccggcct ggccaacgac 1621 tacaacaaga gaaccatggg cctgctggtg agcgacatcg gcaccatcgt gtggggcacc 1681 accgccgccc tgagcaaggg ctacgtgaga gtgatcttct tcctgatggg cctgtgctac 1741 ggcatctaca ccttcttcaa cgccgccaag gtgtacatcg aggcctacca caccgtgccc 1801 aagggcagat gcagacaggt ggtgaccggc atggcctggc tgttcttcgt gagctggggc 1861 atgttcccca tcctgttcat cctgggcccc gagggcttcg gcgtgctgag cgtgtacggc 1921 agcaccgtgg gccacaccat catcgacctg atgagcaaga actgctgggg cctgctgggc 1981 cactacctga gagtgctgat ccacgagcac atcctgatcc acggcgacat cagaaagacc 2041 accaagctga acatcggcgg caccgagatc gaggtggaga ccctggtgga ggacgaggcc 2101 gaggccggcg ccgtgaacaa gggcaccggc aagtacgaga gcagcatcga tatggtgagc 2161 aagggcgagg aggtcatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccatg 2221 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag 2281 accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc 2341 ccccagttca tgtacggctc caaggcgtac gtgaagcacc ccgccgacat ccccgattac 2401 aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc 2461 ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg 2521 aagatgcgcg gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc 2581 tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac 2641 caggccctga agctgaagga cggcggccac tacctggtgg agttcaagac catctacatg 2701 gccaagaagc ccgtgcaact gcccggctac tactacgtgg acaccaagct ggacatcacc 2761 tcccacaacg aggactacac catcgtggaa cagtacgagc gctccgaggg ccgccaccac 2821 ctgttcctgg ggcatggcac cggcagcacc ggcagcggca gctccggcac cgcctcctcc 2881 gaggacaaca acatggccgt catcaaagag ttcatgcgct tcaaggtgcg catggagggc 2941 tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc 3001 acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc 3061 ctgtcccccc agttcatgta cggctccaag gcgtacgtga agcaccccgc cgacatcccc 3121 gattacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag 3181 gacggcggtc tggtgaccgt gacccaggac tcctccctgc aggacggcac gctgatctac 3241 aaggtgaaga tgcgcggcac caacttcccc cccgacggcc ccgtaatgca gaagaagacc 3301 atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag 3361 atccaccagg ccctgaagct gaaggacggc ggccactacc tggtggagtt caagaccatc 3421 tacatggcca agaagcccgt gcaactgccc ggctactact acgtggacac caagctggac 3481 atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgctc cgagggccgc 3541 caccacctgt tcctgtacgg catggacgag ctgtacaagt aggctagccc cgaattcgat 3601 atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt 3661 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat 3721 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct 3781 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct 3841 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc 3901 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg 3961 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc 4021 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttaactgga 4081 acaactgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 4141 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 4201 gggccgcctc cccgcatcga taccgtcgac ctcgacccgg gcggccgctt cgagcagaca 4261 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct 4321 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac 4381 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg 4441 ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat cttcctagag 4501 catggctacg tagataagta gcatggcggg ttaatcatta actacaagga acccctagtg 4561 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag 4621 gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagcctt 4681 aattaaccta attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt 4741 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag 4801 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc 4861 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 4921 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc 4981 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta 5041 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc 5101 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 5161 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt 5221 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 5281 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa 5341 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac 5401 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg 5461 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc 5521 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg 5581 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga 5641 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc 5701 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag 5761 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga 5821 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg 5881 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga 5941 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt 6001 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact 6061 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt 6121 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg 6181 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta 6241 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac 6301 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta 6361 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt 6421 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt 6481 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt 6541 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc 6601 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg 6661 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg 6721 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt 6781 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac 6841 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg 6901 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg 6961 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat 7021 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt 7081 tacggttcct ggccttttgc tggccttttg ctcacatg //