LOCUS       Ple103\(pEMS1083       18322 bp    DNA     circular     26-JAN-2009
SOURCE      
  ORGANISM  
COMMENT     PCR genotyping:
            Sense primer: oEMS2364  5'- GCGTATCACGAGGCCCTTTC -3'  Tm= 56.9 Deg C
            Antisense primer: oEMS2379  5'- AGTTCGCTTTGCCCTCCTCTT -3' Tm=57.3 Deg C
            
            Cycling conditions:
            Step 1: 94 Deg C for 3 min
            Step 2: 94 Deg C for 1 min
            Step 3: 61 Deg C for 1 min
            Step 4: 72 Deg C for 45 sec
            Step 5: repeat Step 2-4 34 times
            Step 6: 72 Deg C for 5 min
            
            338 bp product
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|508256201|
COMMENT     VNTDBDATE|508256201|
COMMENT     LSOWNER|
COMMENT     VNTNAME|Ple103 (pEMS1083)|
COMMENT     VNTAUTHORNAME|Russell Bonaguro|
COMMENT     VNTOAUTHORNAME|Demo User|
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COMMENT                "@N" 0) 
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COMMENT                (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #139=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 
COMMENT                1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #140=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 
COMMENT                1 1 "@N" 0) 
COMMENT          #141=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
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COMMENT                5 "@N (@S)" 0) 
COMMENT          #142=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 
COMMENT                "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 
COMMENT         (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 
COMMENT          (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 -365) 
COMMENT        1 1 0 1 1 
COMMENT        (mapper: 23.4882 -17.489 40.0378 40.0378 0.01 10 -1 18322 18322 1 0 0) 
COMMENT        #143=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 1937009781 100) 
COMMENT              (CObjectList 
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COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 58 22 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 
COMMENT                            1.74233 0.833333 0 "Ple103 (pEMS1083)" "@N" 1 0 
COMMENT                            0.871165 0 -10 11.4392 1.47361 Nil) 
COMMENT                      #147=(CLabel 
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COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 
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COMMENT               #148=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -116 100) 
COMMENT                     (CObjectList 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
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COMMENT                                   "MFRT (F5 mutant)" "@N" 1 9.9902 12.6579 0 
COMMENT                                   -10 8.21723 1.17389 #150#) 
COMMENT                             #152=(CScratch 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Kozak consensus sequence" "@N" 1 13.3549 
COMMENT                                   11.4401 0 -10 12.7629 1.17389 #152#) 
COMMENT                             #154=(CWideArrow 
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COMMENT                                   7.13994 9.54791 0 -10 2.67247 1.17389 #154#) 
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COMMENT                                   11.2976 9.32914 0 -10 1.6984 1.17389 #156#) 
COMMENT                             #158=(CScratch 
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COMMENT                                   13.2203 7.53685 0 -10 1.99811 1.17389 #158#) 
COMMENT                             #160=(CScratch 
COMMENT                                   (CWidget 0 (3 #6# 0) 1 2 0 0 #112# 1152 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.22035 1.9 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                   "SV40 early polyA signal" "@N" 1 17.1005 
COMMENT                                   2.12901 0 -10 11.0645 1.17389 #160#) 
COMMENT                             #162=(CWideArrow 
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COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                             #163=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "HPRT complementary sequence" "@N" 1 18.3695 
COMMENT                                   -3.48466 0 -10 14.9609 1.17389 #162#) 
COMMENT                             #164=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #8# 0) 1 2 0 0 #108# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "3' homology sequence" "@N" 1 6.32469 
COMMENT                                   -12.1963 0 -10 10.4901 1.17389 #164#) 
COMMENT                             #166=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #9# 0) 1 2 0 0 #108# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                             #167=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                   "5' homology sequence" "@N" 1 -16.4857 
COMMENT                                   1.74233 0 -10 10.4901 1.17389 #166#) 
COMMENT                             #168=(CScratch 
COMMENT                                   (CWidget 0 (3 #12# 0) 1 2 0 0 #112# 929 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 2.10807 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #169=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #104# 36406172 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "I-Sce I" "@N" 1 
COMMENT                                   -11.9574 -6.96932 0 -10 3.02214 1.17389 #168#) 
COMMENT                             #170=(CWideArrow 
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COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                                    6.22594 1 0.082322) 0.8 1.8 0) 
COMMENT                             #171=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Ple103 (HAP1-A)" "@N" 1 6.36328 15.1435 0 
COMMENT                                   -10 7.49292 1.17389 #170#) 
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COMMENT                                   "@N" 1 6.51327 13.9007 0 -10 5.86945 1.17389 
COMMENT                                   #172#)) (CObjectList)) 
COMMENT                      #174=(CGroupWidget 
COMMENT                            (CWidget 22 (7 22 0) 1 2 0 0 Nil -1093 100) 
COMMENT                            (CObjectList 
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COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.28438 1.9 
COMMENT                                   0.082322 1) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "MFRT" "@N" 1 
COMMENT                                   14.378 5.12016 0 -10 2.72243 1.17389 #175#) 
COMMENT                             #177=(CWideArrow 
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COMMENT                                    (CWidget 0 (3 #10# 0) 1 2 0 0 #120# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 0.835356 
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COMMENT                             #178=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                   -5.9711 11.6219 0 -10 2.87229 1.17389 #179#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #181=(CGroupWidget 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "oEMS2364" "@N" 
COMMENT                                   1 -6.10192 13.2583 0 -10 4.79547 1.17389 
COMMENT                                   #182#) 
COMMENT                             #184=(CScratch 
COMMENT                                   (CWidget 0 (3 #16# 0) 1 2 0 0 #112# 1217 100) 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "oEMS2379" "@N" 
COMMENT                                   1 -4.38666 14.7379 0 -10 4.79547 1.17389 
COMMENT                                   #184#)) (CObjectList))) (CObjectList)) 
COMMENT               #186=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -325 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #187=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil 956 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #188=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -255 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 
COMMENT        (CObList #189=(AnlzFontBackColorItem 53 72 43633766) 
COMMENT         #190=(AnlzFontBackColorItem 138 348 40304639) 
COMMENT         #191=(AnlzFontBackColorItem 370 1014 40304639) 
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COMMENT       (CStringList) 0 851972 (CObList)))
FEATURES             Location/Qualifiers
     misc_feature    1024..1057
                     /vntifkey="21"
                     /label=MFRT\(F5\mutant)
     misc_feature    1058..1069
                     /vntifkey="21"
                     /label=Kozak\consensus\sequence
     misc_feature    1070..1786
                     /vntifkey="21"
                     /label=EGFP
     misc_feature    1787..2812
                     /vntifkey="21"
                     /label=Cre
     misc_feature    2813..2885
                     /vntifkey="21"
                     /label=NLS
     misc_marker     2889..2922
                     /vntifkey="22"
                     /label=MFRT
     misc_feature    3076..3126
                     /vntifkey="21"
                     /label=SV40\early\polyA\signal
     misc_feature    3132..7085
                     /vntifkey="21"
                     /label=HPRT\complementary\sequence
                     /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2"
     misc_feature    7086..10731
                     /vntifkey="21"
                     /label=3'\homology\sequence
                     /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3"
     misc_feature    12185..15996
                     /vntifkey="21"
                     /label=5'\homology\sequence
     misc_marker     10733..12159
                     /vntifkey="22"
                     /label=AmpR
     misc_marker     complement(17316..18316)
                     /vntifkey="22"
                     /label=AmpR
     misc_feature    12166..12183
                     /vntifkey="21"
                     /label=I-Sce\I
     misc_feature    139..1015
                     /vntifkey="21"
                     /label=Ple103\(HAP1-A)
                     /note="1302"
     misc_feature    139..1015
                     /vntifkey="21"
                     /label=Prom\region
     primer          54..73
                     /vntifkey="27"
                     /label=oEMS2364
     primer          350..370
                     /vntifkey="27"
                     /label=oEMS2379
BASE COUNT     4844 a      4171 c      4221 g      5086 t 
ORIGIN
        1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 
       61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 
      121 cgagctccta ggccggccca cacgccacca tgcttggcta atttttgtat ttttagtaga 
      181 gacagggttt caccaggtgg ccaggcttgt ctggaactcc tgacctcagg tgattcgcct 
      241 gccccggcct cccaaagtgc tgggattaca ggcgtgagcc actgcgcccg gctacaggga 
      301 gacttttaag aggcgtctcc caaggagaac aggggttcgc cagcatggca agaggagggc 
      361 aaagcgaact agggttggag gtgaagaaca gttaatccaa tcaaatggaa gacgtcgagt 
      421 ggcgcgagca ctggggtgtg agcgcgaggg tcagggttgc agcgccaggc agggttgcag 
      481 cgccaggcag ggttgcagcg ccaggcagag ttgcagcgcc aggcagggtt gcagcacgtt 
      541 ggaagcgcac tggagtttgg aatgtctctc ccagtcaacc gggaaagacg cagcgctgcc 
      601 ctaatgcact ggccacccca gaccgtatgc acaacgttgg gttggcagct tctacaccag 
      661 ggaaggcacc cgaaaccccg gcgcggcagc tggggagggg ctgcccccag ccagggaccc 
      721 gcgagtgctg gggcagccgc ggctttgggc agaaccattc tccggagatg gagagggcag 
      781 aaaaaatctt tcgctcccat cccaactcgg agccatccgg aacttgcact cgcccttctt 
      841 gccctgcttt tcttactgtg ctctgttccg tcaccactag ctcccctccc gcgcggctct 
      901 gagtcccgct tgccactagt tgcttctcgg cagcccccac ccctacgcgt ggtccggccc 
      961 ggcggctggc gcaggttata aaggtcccgc cgcagcagcg gacggcagac tcgagggcgc 
     1021 gccgaagttc ctattcttca aaaggtatag gaacttcgcc gccaccatgg tgagcaaggg 
     1081 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg 
     1141 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct 
     1201 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct 
     1261 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt 
     1321 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg 
     1381 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga 
     1441 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa 
     1501 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa 
     1561 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca 
     1621 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca 
     1681 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt 
     1741 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagttatcca atttactgac 
     1801 cgtacaccaa aatttgcctg cattaccggt cgatgcaacg agtgatgagg ttcgcaagaa 
     1861 cctgatggac atgttcaggg atcgccaggc gttttctgag catacctgga aaatgcttct 
     1921 gtccgtttgc cggtcgtggg cggcatggtg caagttgaat aaccggaaat ggtttcccgc 
     1981 agaacctgaa gatgttcgcg attatcttct atatcttcag gcgcgcggtc tggcagtaaa 
     2041 aactatccag caacatttgg gccagctaaa catgcttcat cgtcggtccg ggctgccacg 
     2101 accaagtgac agcaatgctg tttcactggt tatgcggcgg atccgaaaag aaaacgttga 
     2161 tgccggtgaa cgtgcaaaac aggctctagc gttcgaacgc actgatttcg accaggttcg 
     2221 ttcactcatg gaaaatagcg atcgctgcca ggatatacgt aatctggcat ttctggggat 
     2281 tgcttataac accctgttac gtatagccga aattgccagg atcagggtta aagatatctc 
     2341 acgtactgac ggtgggagaa tgttaatcca tattggcaga acgaaaacgc tggttagcac 
     2401 cgcaggtgta gagaaggcac ttagcctggg ggtaactaaa ctggtcgagc gatggatttc 
     2461 cgtctctggt gtagctgatg atccgaataa ctacctgttt tgccgggtca gaaaaaatgg 
     2521 tgttgccgcg ccatctgcca ccagccagct atcaactcgc gccctggaag ggatttttga 
     2581 agcaactcat cgattgattt acggcgctaa ggatgactct ggtcagagat acctggcctg 
     2641 gtctggacac agtgcccgtg tcggagccgc gcgagatatg gcccgcgctg gagtttcaat 
     2701 accggagatc atgcaagctg gtggctggac caatgtaaat attgtcatga actatatccg 
     2761 taacctggat agtgaaacag gggcaatggt gcgcctgctg gaagatggcg atgatccaaa 
     2821 aaagaagaga aaggtagatc caaaaaagaa gagaaaggta gatccaaaaa agaagagaaa 
     2881 ggtaataaga agttcctatt ctctagaaag tataggaact tcagcggccg cgactctaga 
     2941 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc 
     3001 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag 
     3061 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 
     3121 cactgcgaat tctcgtaaaa ctcttcatgg cagtagttat tattctctct ctctctcttt 
     3181 ttcttttttc ttgagacagg atatttctct gttgcccagg ctggagtgca gtggcacagt 
     3241 cttggctcac tgcagcctgg acctcctggg ctcaagccat cctcccacct cagcctccca 
     3301 agtagctggg gctacaggca catggccacc aggcccagat aatttttcat ttttgtagag 
     3361 actgagtctc accatgttac ccaggtttat tattctcatt ttttagatga agagactgag 
     3421 gtccagagaa gctcaatgac ttgcctagtt ttacaaatct cctgccatca catacccctc 
     3481 agcgtcctta ataagaggga ggccaccaac tatgtgctgg gcactgtggt ggatgctgga 
     3541 gctatagggt tgagtatata agaaatggtg ttgctggagc aactgttgct tgcttacctg 
     3601 acctatctga gaattaatta gcaggggaac atatttttgt tttcagattc aatataagaa 
     3661 cttgtgtggg caaaaataaa gatcagtagt aataacagta gttcccattt gctgactgta 
     3721 ctgtcctaag tgcatatata tatacataca cacacgcata cctatactcc tctaatactc 
     3781 aaaatgatcc tgtttatgta ttgttaatat gctcatttta tttttaaatt tttatttatt 
     3841 tttattttta tttatttttg agacggagtc tcattctgtc gcggaggctg aagtgcagtg 
     3901 gtgcgatctc agctcagtgc gacctccgcc tcccgggttc aagtgattct cctgcctcag 
     3961 cctccggact agctgggatt acaggcgccc gcctccacgc ccagctaatt tttgtatttt 
     4021 tagtagagat ggggtttcgc catgttggcc aggctggtct cgtactcctg accttgagtg 
     4081 atccacctgc ctcggcctcc caaagtgctg ggattacagg catgagccac cgcgccgggc 
     4141 taatatgctc attttagtga ggcaaaaata gaggctcaga gtctgatttg tacaaaacta 
     4201 cagagcagtt aagtgtcctc tcagatgtgt accctgatct gggtgactct aggactctag 
     4261 gtctcaactg ttacaaccag ttaagggttt ggggaagcac tgggccaaga gtcaggaaaa 
     4321 tggaagccac aggtagtgca aggtcttggg aatgggacgt ctggtccaag gattcacgcg 
     4381 atgactggaa cccgaagagc cggggcccgg tttacggccg ccatgaagca acgcgcgccg 
     4441 gtaggtttgg gaatcaggga gccctctgaa taggagactg agttgggagg gaaaggggct 
     4501 tcgctggggg agcctcggct tcttctggga gaaaattccc acggctacct agtgagcctg 
     4561 caaactggta ggcgccggcg taggcgcgcg ggcggggccg ggggcggggc ctgcggggcg 
     4621 tggcggggcg ggcagagggc ggggcctgct tctcctcagc ttcaggcggc tgcgacgagc 
     4681 cctcaggcga acctctcggc tttcccgcgc ggcgccgcct cttgctgcgc ctccgcctcc 
     4741 tcctctgctc cgccaccggc ttcctcctcc tgagcagtca gcccgcgcgc cggccggctc 
     4801 cgttatggcg acccgcagcc ctggcgtcgt ggtgagcagc tcggcctgcc ggccctggcc 
     4861 ggttcaggcc cacgcggcag gtggcggccg ggccctgagg cgcgggatcg atccatgaca 
     4921 cccccccgcc ccccatatct cactataatt cactgtcaaa tggcttttat gatttggagt 
     4981 ttggggatac ttttaaagac agggtcttgg tagctcctag atggcttggg attcacttag 
     5041 taacccttgc tggcctaaaa ttcaggaact tctacctcat cttcccaagt actgggttag 
     5101 tagggatgtc cagcatattt agcttgcttt aatgattttg ctgtactttt ttctgtgtgt 
     5161 acaggtgcat gtatatttat aaaactaaca taatatattt tacccccaaa gtaccataac 
     5221 cctaattaat gctattaaat gttttgtatg ctgggctctg ggcatactaa tataaccaat 
     5281 attatacaat gcaaaacaat tttggaatat cttctgtaaa attatcttat tagtaagttg 
     5341 taaccatctc agacttctaa gtttgagttt gtaaaggatc agtttttttt ggtgacctac 
     5401 acacacacac acacacacac acacacacac acacacaggg gggtggattc tgagaattta 
     5461 gagaatttag atggttaata ccagttttta tttttattct gtgtgatatt tatttattaa 
     5521 gatttattta tttattacat gtaggtacac tgtatctgtc ttcagacaca ccagaagagg 
     5581 gcatcagatc tcgttacaga tggttgtgag tcaccatgtg gttgctggga tttgaactca 
     5641 ggacctctgg aagaacagtc agtgctctta accgttgagc catctcacca gccccgtgat 
     5701 attttattta acttactaga atatactgtg ttaaaacata ttttagggga ttggggagat 
     5761 ggcccattct gtaaagtgtc tgccatgtca tcttcaggac ctgcgtttgt aaccccagca 
     5821 tggagaagac agagacaggg agacccctgg gcttcctggc aagctagact agcaaatttg 
     5881 gttaactagg ttcaatgaga gactcagtct caaaacataa gaagtgaatt ttttagaccc 
     5941 aatttcaatc tctgaactcc atacgtatgc acatgtacct gcatataaat atatatacac 
     6001 acccataaac acatgcacac acaaaaaatg gttcaaaagt tcttaaaatg tataagagta 
     6061 gaaagtgtag agccgggagt ggtggcgcac gcctttaatc ccagcacttg ggagcagagg 
     6121 caggtggatt tctgagttcg aggccagcct ggtctacaga gtgagttcca ggacagccag 
     6181 agctatacag agaaatcctg cctcaaaaaa ccaaaaataa agtgtatata ctaatctttc 
     6241 atgcctaaaa tatttatttt tgccacattt ttcatacctc tttaatattt ttctttttct 
     6301 ctttgtgctt caaaggaaat tttgtgtcat gttttacttt tcattcttga ctctattcag 
     6361 cagtaagacg cagcatggct ttcttagaga agtataatct attacaaaac ggtaacattt 
     6421 aactgctcta cataccctct tgtagctaaa ttcctctgtt aaactaagac ttattatcta 
     6481 aacgtaatta aatgttgata tcttataatg ctccactttg aaacagctgt ctttcttttg 
     6541 cagattagcg atgatgaacc aggttatgac ctagatttgt tttgtatacc taatcattat 
     6601 gccgaggatt tggaaaaagt gtttattcct catggactga ttatggacag gttagtaaga 
     6661 cctcgattga agttattttt agagggtgtg tgtgtggtgt gtgtgtgtgt gtgtgtgtgt 
     6721 gtgtgtgtgt gtgtgtaaga gagagaggag aggcatgtgt gatatatatg tgaagtgtat 
     6781 tgcatgtaca tggaatccag agaaggacat taggtgtcct actctgttgc tctcttattc 
     6841 ccttgaatca gtgtctcttg ctgaacctgg ggcatacata ctgatttggc taggctgcct 
     6901 ggctagcaag acccagggat ctgcttgtct ctgcccccca gtgctgacgt tacaagcagg 
     6961 tgcaaccaca tctggttttt atatgggtac tgggggatct gaacttaggg cctgatgcgt 
     7021 gcccaagtgc tctcacccgc caagccacct ctccagccaa agggtttttt ttcttgtatt 
     7081 aattctaact tagcaactgt tgagtagttg atgtttgaat ataagctaag ccaacttatt 
     7141 agaataaatg ttttctattt tttacttgta tgtgtgagtg cctgcatgta tgtatgtgaa 
     7201 gcatatgtat tccaggatgt cagaattgtg atggattccc tggaactgga gttacagacc 
     7261 attgtgaacc actgtgtatg ggtgctggga actgaacttt aggtcctctg caaaaacagt 
     7321 aaggatctta actgataaac tatctttcta gccctaaggc tttctacttt taaagttcgt 
     7381 ttctctttct ctctctctct ctctctctct ctctctctct ccctctccct ccctcctcct 
     7441 cctcctcctc tttcccccct ctcgcctcct ctttctctct cccctccctc tctctctctc 
     7501 acacacacac acagacagag agaggagaga gaaaggaagc cttactaatt aattacctag 
     7561 ttttagacat aactagtggg aatccatagt aagtaaatca acattcttga agagaggtta 
     7621 gaagaattgg ctctcgttgt ccatcgaggc tgtataagat tattcttctg ttttctcagc 
     7681 ttaccataca tattttctac gtagacaata ccaattcaaa tacactgtac gtaaagtata 
     7741 tgcgcacaca cacacacaca cacacacaca cacacacaca agttacatat tgtcatatag 
     7801 tactaaaatt tatggacttt ttcatattaa tacatctaga gctatcatgt tttttatctt 
     7861 ttaaaaatta ctttacgggg ctacagagat gtctcagcag ttaggagcac tgactgctct 
     7921 tccagaggtc ctgagttcaa ttcccagcaa ccacatggtg gctcacaacc atctgtaatg 
     7981 ggatctgatc cactcttatg gtgtgtctga agacagtgac agtgtactca tatacatgaa 
     8041 ataaatcttt aaaaaattac tttaccatca gggtatatat atatatatat atgtgtgtgt 
     8101 gtgtgtgtgt gtgtatgaag tatttgctat ttgagagggt ctcactgttt agctcagtct 
     8161 ggcttggaac gttctgtata gcccagactg gcctcaactc agcagtcccc ctgaaaggct 
     8221 ctcatatgtg gagattacag gtatgtgcta ctgtgcccag ccctacatgt tactttgttt 
     8281 ttgaaatagg gtatcaaata gcttagactg gcttctaagt cagtgtgtag ctaaggatgg 
     8341 tctgagcttc tgaacctgct acttcaactc ctggtgtgcc atcatggcca gtttgcacag 
     8401 ttgtgagagt agaactcagg acctcctatg tgttactaga catgttctct accagctgag 
     8461 ctacatccca attcgcctac atttactttt attttttaaa agatttattt atgtatacag 
     8521 cactctgcct gcatgtacat ctgcatgcca gaagagggca ccagatctca tcatacatag 
     8581 ttgtgagcca ccatgtggtt gctggggatt gaactcaggg cctctggaag agcagtcagt 
     8641 gctcttaacc tctgagccat ctctgtagcc ccctacattt attttaaaga ttataggcta 
     8701 ttctattcat agatgtgcca taactgattt aaccagatct tcattatttt gtacttagta 
     8761 gagtatatta gagggaatga atcttaaata cacattttga cacttgctca gctttagaca 
     8821 agtaaaaatc actggtcaag gaaatggtgc ttagtggatg cttctgaact gtcaaattgt 
     8881 cctccaacaa gttacatcat tttatacttc caacgttatg tgagagttca tttttcttta 
     8941 ggctcttcta gtattttata tttgagaaat aaaatttaac caaaatttgc caatctagta 
     9001 agcttgtttt tatttgtatc tttaaatttt atgatagatg ttatagtgta ctctcctctc 
     9061 ccctcccctc ccctctcctc ctcagcagga tcttgttatg tagccctaag ggatctaata 
     9121 cttactctgt agtccaggct gtccttcagc tcatgagaca caccatcatg cttggcatgt 
     9181 ttcttgttct gaaaggtatc tggttttaat tttggttgag ctggtgtgtt ggtgtatatc 
     9241 tgtaatctca gcacaggcaa gtagagacaa ggattatgat tacagtccag cctgggctat 
     9301 ttagagggat tctctttaat ctttcctcat gccccaaaat cttacctttg gtatatgaaa 
     9361 aatagtctcc acttctgcaa aatattgctt tatgaagtaa gaattaattc ccttcataga 
     9421 gacaaggaat gtgtcctgta aaagtttaat gtgtaagaag tatttgttat aaaagataaa 
     9481 tattcagaat cttcttttta attcctgatt ttatttctat aggactgaaa gacttgctcg 
     9541 agatgtcatg aaggagatgg gaggccatca cattgtggcc ctctgtgtgc tcaagggggg 
     9601 ctataagttc tttgctgacc tgctggatta cattaaagca ctgaatagaa atagtgatag 
     9661 atccattcct atgactgtag attttatcag actgaagagc tactgtgtaa gtataattaa 
     9721 cttataatta aaaaaatagg gccattctag ttttatctat atttttttta aacttgtgca 
     9781 aactatgcta cataatcaac ttacttgatt atgtaatata tatagtaata gttggaatta 
     9841 ctattattac atatttattt ttctgtgctg gggtagaacc cagggaacta agtactgtaa 
     9901 taaccgagct tcattcatcc tagtcatttt taattttttt tttttttttt gttttttcga 
     9961 gacaaggttt ctctgtgtag ccctggctgt cctggaactc actctgtaga ccaggctggc 
    10021 ctcgaactca gaaatctgcc tgcctctgcc tcccagtgct gagattaatg acgtgcacca 
    10081 ccactgcctg gcattttttt aaagttttaa tttgagaccc tttctcacta acttgctcac 
    10141 actatcctcg aactcattac gtgggctggt ctagaaattt cagtcaactt gcctcagctt 
    10201 ctcaagtagc tgggattaca ggcaggtgct accacttctg gccatgatca ttgttgttgt 
    10261 tgctattatt attattatta tttcactgat acttcatatc acaacacaga atagtaactg 
    10321 tagttcatga ccaacaaaaa agttgtacag tttctctgtt gtcttatttt gatctggatt 
    10381 ttgatttgtt tcatggcact gggcaggcta atctcaaact gcatggctca aatagtcctt 
    10441 ctgtcttaat ctcccatgtt ctggaagtat aggtatcact acacctagct gtattttcat 
    10501 attttaaaaa aaacgcctaa gaattgctat tgaatgaggc ggagagatgg ctcagcagtc 
    10561 acaagtactt gtagaggact ggggtttggt tactagcacc tcaaagatgg cttacaatta 
    10621 tctgtagctc caattccaga ggatctgtgt ggtaaagctg catgtatatg ggcatgcagg 
    10681 taaaactgct aaacatataa aaatagctca gtcttaagag aattggtcga ctgtcgactt 
    10741 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga 
    10801 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg 
    10861 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata 
    10921 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 
    10981 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 
    11041 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc 
    11101 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa 
    11161 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg 
    11221 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag 
    11281 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc 
    11341 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta 
    11401 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg 
    11461 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca 
    11521 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac 
    11581 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat 
    11641 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg 
    11701 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata 
    11761 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta 
    11821 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa 
    11881 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag 
    11941 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 
    12001 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 
    12061 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 
    12121 taatctgctg cttgcaaaca aaaaaaccac cgctaccagg tcgagtaggg ataacagggt 
    12181 aatggatcct cgttgggcac ctgcactcat gtacgcaggc ccacatatag atgtacacac 
    12241 gcatatcatt caaaatagaa attatttata caagtagtgc gttggagagc tggctcagga 
    12301 gttgttaatg cttgctaccc ttgcaaagga cccaagttca atttccagca cctgtgttgg 
    12361 gcatctcaca accacatgta aatctagttt caggggattt cacccctctt ctgaactctt 
    12421 caggcactta tacccacaaa tggacatctc cacatagaga cacatacttt taaaaaagta 
    12481 atctagggtc tagagaaatg gttatagacc acggactggg atcagttccc agaagccaca 
    12541 tcctgtggct cacaattgcc tgtgaatcaa gttctagatc tggtggcctc gtctagcctg 
    12601 cagtcatcaa caaacacatg cacacatcca tacacacata atttaaaaga aagcgaaagt 
    12661 aacctataat gctaaacaaa gcacattctc agtgtagact gcagtggtga tgaaccttga 
    12721 aaatatatta atagaagcca gacacgtaag tcacaaatgg tataactcaa tttatatgaa 
    12781 attcccagag agtccaaagt taaaaaaaac aaaaaggcag attattggtt gtcagtagct 
    12841 acagagatgg gacattgaag atattaatag gtatagggtt gaggattcat ggcctttccc 
    12901 tcttccatgt tagcatgtct attgttgtta accttattca actcatgtta gggcaatcat 
    12961 attgatgaga cttaatgagt atagcttgac attcctagaa gacagagtct cacatcagac 
    13021 tctctgatcc tctgactctt agaatctttc aacaccctct tctgaaatga tctctgagcc 
    13081 tttggttgtg actgaattat gagaaagaat tttatgttga atgaatttta cttcagcatt 
    13141 tttacaaaac tgaagctggg gatggcgttt catgcgtgca gtccctacca tttgcctata 
    13201 agcctggagg aggcagaagt agaattaggc ttttcaggga catcttcagc tgcatcatga 
    13261 gcttgaggac aacttgagct acataagatt cccatcccca cccccacaca cacacacaca 
    13321 tctagtatgt acctagtatg cagtcttttc atatgtttaa aatgtagaaa ggtatatgtg 
    13381 aagtctccct tttccccaca acttagtccc tccaaagtat tgcatcagtt tccttgtaca 
    13441 tgattttatg catatataag atacactatt tctctctctc tctctctcct tccctccttc 
    13501 cctccaacct ctgggtatga gttgagggtt atgtatgttt caagttgtaa taagaattgc 
    13561 cactcaaaca tttcatccca ttttaagtta agtttgtctg attttccact ttagaagggc 
    13621 agccatgatg gctcatgcct ataatcccat cctagcactt gtaagcctaa tacataagac 
    13681 tggaggattg tcatcagctt gaggctagcc tggtctacct aatgagttcc agccagccaa 
    13741 gagtagaata taagaccctg tctccaaaaa atgtaccaaa ggggctggag gggtaactca 
    13801 gtggttaaga acagtagctg ctcttccaga ggacctgggt tcaattccca gcacccacat 
    13861 ggcagcttgc aactatctgt aactgtccag ctttgggata tccgacacca ttataccaat 
    13921 tacataaaat gaaaaaattg taaaatgtgc taaaatgagt ggggaggggg agcatggtgt 
    13981 ggttctgtgg gcttgggatt ttgccatgaa tccatgacta tatctcagca atatgtggat 
    14041 tatttctgga aacctgtggg gcatgggtgt atgggaagag gggagagaga gagagcccgc 
    14101 atccagccag agttcctgtg ctctgggcag gcggacgtgg gaggactgct ggacgatttc 
    14161 cacttggccc tgggtgggca tctggctgtg tgaagccact gaccccactc agcggggggt 
    14221 agataagggg catttcccat accaggttct cagactccag cattccacgc tggatacagc 
    14281 agaagagctg agaacagaat aggggcccca gggagacact ctcgtcccag agataaagaa 
    14341 ggcagaggga gagagattcc tacgcaggcg agaggcctta gtctgggcct tgatgaacag 
    14401 agacggtcta tggttttaga gctttattgt agaaaggcag ggggaaagag agaaggtaga 
    14461 aagagagaga ccagccatgg ccaagaggag agaagggggg aaaggagaga gagaagaaag 
    14521 gctagagagt aagaaaggtt aaagagacag aagtaaaagc ttagagtaag agagagagag 
    14581 agagcaagag ggagagtaag aaaagtaaaa gcttaagaga gcgaggaggg gccagcccct 
    14641 ctaatagtgg gcttgttatc ttgttgttgc taggtaactg tgaagcgtct agcctgaagg 
    14701 ccagaagctt gggacattgt ctatgtgact gaaagccatg agcctctact gtgggggctg 
    14761 tggaggtggt aacttggaca ggagccaggg gttccaggag acatgaggga atgccttctg 
    14821 tcccatgtag gtaggaatta ccaccaccgg ctttcaccgt agttcactgg agactgtttg 
    14881 tacatagccc attgccccac agaaaccagg cactcactgt ttcatttgca taagaattag 
    14941 aaggctttat tttggctggg attggtgact tgccccagaa attccagctt ttgggaagcc 
    15001 gaatcctgga gaaccaggag ttcaaggtca tcctcaggta cctcatttgt tcaagaccag 
    15061 cctgtgctac catgagacct tgtccctact cccctcaacc ccaaaacacg ctgttttcat 
    15121 tttataggtt gtactaacca tggaggatgg tcatttgcct ttctcttgat ttcagaagcg 
    15181 tagcgcgggc tttgcaccat gatctagtgt ttccagatag gcttgcctct ccacagccag 
    15241 gcttatcctg cttcagtccc atgtttggca agggcaacat atgactgaag aacttgcata 
    15301 gccttatggt gtatcatgac tcttagcccc agtttataac tgtctaacag ctggtggctt 
    15361 ttgtgaaaaa ggaaagcatc atttgaactt ttaacttttc agctgtttgt tttgactttg 
    15421 cataaatact gagactagct aggaaatgag cttttgtttc atttgctaaa aatgattttt 
    15481 ttttcatgag gcaatttcaa gcaatttgaa tttagcaatc tctacttgtc tgaaaagaag 
    15541 ttattaatta aaaaaaaaca aacaaacagg aacctattat gctggctagt cacatgaaag 
    15601 aatccaaatg aacacagtca aggctgaatc aacacatgaa attattttct aattccccaa 
    15661 attacattta tgctaattag tatgtagtaa aacattttaa ttgtttccaa tgttaaacat 
    15721 tacagaaata attttgatgt tttcctaata acccagcctt tgatcatgtt tgcatttatt 
    15781 tgattgttgt cagtttatta aataattaag taagagcaac aagataaaat gacttcatga 
    15841 gagtcctttt ctgtaactta gaacgtcagt agtcatagga actgcggtcg ttcccttacc 
    15901 tttcagaagt aggctaaaga gttgaacgca aaggtggttt ttcgaatctt cctgatcctt 
    15961 aagcccccac taggtttata ttctatattt gatatcggat tcgaacccct cgagcttagg 
    16021 atccggaacc cttaatataa ctaagttatt aggtccctcg aagaggttca ctagtactgg 
    16081 ccattgcggc cgcttcgagg ctgcctcgcg cgtttcggtg atgacggtga aaacctctga 
    16141 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa 
    16201 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca 
    16261 cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga 
    16321 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca 
    16381 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 
    16441 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 
    16501 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 
    16561 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 
    16621 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 
    16681 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 
    16741 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 
    16801 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 
    16861 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 
    16921 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 
    16981 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 
    17041 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 
    17101 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 
    17161 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 
    17221 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 
    17281 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 
    17341 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 
    17401 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 
    17461 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 
    17521 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 
    17581 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 
    17641 ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 
    17701 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 
    17761 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 
    17821 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 
    17881 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 
    17941 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 
    18001 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 
    18061 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 
    18121 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 
    18181 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 
    18241 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 
    18301 cccgaaaagt gccacctgac gg 
//
