Name type location ---------------------------------------------------------------------------- M13 fwd primer_bind 50..66 T7 promoter promoter 73..91 Peft-3 promoter 127..735 3xFLAG CDS 750..815 SV40NLS CDS 822..842 Cas9 CDS 867..1907,1959..2945,2997..4058,4110..5120 mEGFP CDS 5127..5429,5483..5557,5609..5728,5780..5995 egl-13 NLS CDS 6002..6076 tbb-2 UTR 3'UTR 6086..6428 T3 promoter promoter rev:6517..6535 M13 rev primer_bind rev:6556..6572 lac operator protein_bind 6580..6596 lac promoter promoter rev:6604..6634 ori rep_origin rev:6958..7546 beta-lactamase CDS rev:7717..8577 bla promoter rev:8578..8682 f1 ori rep_origin rev:8708..9163