LOCUS       pWPT-ACC/ACC-mEG       10738 bp    DNA     circular     17-OCT-2013
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|676158468|
COMMENT     VNTDBDATE|676158468|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pWPT-ACC/ACC-mEGFP-IRES-mCherry|
COMMENT     VNTAUTHORNAME|Josh|
FEATURES             Location/Qualifiers
     misc_feature    17..39
                     /vntifkey="21"
                     /label=TIS:\\ACC/ACC
     CDS             34..762
                     /vntifkey="4"
                     /label=mEGFP
     RBS             790..1365
                     /vntifkey="32"
                     /label=IRES
                     /note="Internal Ribosome Entry Site (IRES)"
     CDS             1393..2103
                     /vntifkey="4"
                     /label=mCherry
     enhancer        2112..2710
                     /vntifkey="9"
                     /label=WPRE
     primer_bind     10320..10437
                     /vntifkey="28"
                     /label=cPPT
                     /note="central PPT of HIV"
     splicing_signal 10198..10200
                     /vntifkey="38"
                     /label=SA
     misc_feature    9602..9806
                     /vntifkey="21"
                     /label=RRE
     misc_recomb     2848..2882
                     /vntifkey="86"
                     /label=LoxP
                     /note="LoxP"
     LTR             2728..3086
                     /vntifkey="19"
                     /label=LTR/SIN
                     /note="HIV LTR with U3 deletion"
     splicing_signal 9031..9034
                     /vntifkey="38"
                     /label=SD
                     /note="5' splice signal"
     misc_feature    8974..9111
                     /vntifkey="21"
                     /label=psi
                     /note="HIV packaging signal"
     LTR             8289..8922
                     /vntifkey="19"
                     /label=LTR
     polyA_signal    7271..8120
                     /vntifkey="25"
                     /label=pA
                     /note="SV40 pA and intron"
     misc_feature    6412..6869
                     /vntifkey="21"
                     /label=gpt
     promoter        5932..6187
                     /vntifkey="30"
                     /label=SV40
                     /note="SV40 promoter/enhancer"
     misc_feature    5643..5643
                     /vntifkey="21"
                     /label=ORI
     CDS             4018..4878
                     /vntifkey="4"
                     /label=AmpR
     promoter        10470..10717
                     /vntifkey="29"
                     /label=EF1-short
BASE COUNT     2758 a      2679 c      2760 g      2541 t 
ORIGIN
        1 tcgagtaact aactaaacca tgggttgatt accatgggcg aattcagcaa gggcgaggag 
       61 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag 
      121 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc 
      181 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac 
      241 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc 
      301 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac 
      361 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag 
      421 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac 
      481 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag 
      541 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc 
      601 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag 
      661 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc 
      721 gccgggatca ctctcggcat ggacgagctg tacaagttcg aataagcatg catctagggc 
      781 ggccaattcc gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa 
      841 taaggccggt gtgcgtttgt ctatatgtga ttttccacca tattgccgtc ttttggcaat 
      901 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct 
      961 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct 
     1021 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc 
     1081 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa 
     1141 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc 
     1201 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg 
     1261 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc 
     1321 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataagcttg ccacaacccg 
     1381 ggatccgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc 
     1441 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc 
     1501 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt 
     1561 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc 
     1621 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc 
     1681 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc 
     1741 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc 
     1801 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac 
     1861 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc 
     1921 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc 
     1981 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg 
     2041 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag 
     2101 taagcggccg ccgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt 
     2161 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat 
     2221 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct 
     2281 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct 
     2341 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc 
     2401 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg 
     2461 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc 
     2521 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac 
     2581 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg 
     2641 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc 
     2701 ccgcatcggg caattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag 
     2761 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa 
     2821 gacaagatgg gatcaattca ccatgggaat aacttcgtat agcatacatt atacgaagtt 
     2881 atgctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc 
     2941 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa 
     3001 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag 
     3061 tcagtgtgga aaatctctag cagcatctag aattaattcc gtgtattcta tagtgtcacc 
     3121 taaatcgtat gtgtatgata cataaggtta tgtattaatt gtagccgcgt tctaacgaca 
     3181 atatgtacaa gcctaattgt gtagcatctg gcttactgaa gcagacccta tcatctctct 
     3241 cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt ctgagtttct ggtaacgccg 
     3301 tcccgcaccc ggaaatggtc agcgaaccaa tcagcagggt catcgctagc cagatcctct 
     3361 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata 
     3421 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt 
     3481 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc 
     3541 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc 
     3601 taatgcagga gtcgcataag ggagagcgtc gaatggtgca ctctcagtac aatctgctct 
     3661 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg 
     3721 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg 
     3781 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc 
     3841 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt 
     3901 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 
     3961 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg 
     4021 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt 
     4081 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga 
     4141 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa 
     4201 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt 
     4261 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt 
     4321 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc 
     4381 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga 
     4441 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat 
     4501 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct 
     4561 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc 
     4621 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg 
     4681 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc 
     4741 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 
     4801 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca 
     4861 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta 
     4921 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 
     4981 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 
     5041 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 
     5101 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 
     5161 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc 
     5221 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 
     5281 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 
     5341 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 
     5401 cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt 
     5461 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 
     5521 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 
     5581 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 
     5641 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 
     5701 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat 
     5761 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 
     5821 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctgtgga 
     5881 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 
     5941 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 
     6001 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 
     6061 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 
     6121 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag 
     6181 gaggcttttt tggaggccta ggcttttgca aaaagcttgg acacaagaca ggcttgcgag 
     6241 atatgtttga gaataccact ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg 
     6301 gactcatgtg aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt 
     6361 ttcccctcga acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa 
     6421 aaatacatcg tcacctggga catgttgcag atccatgcac gtaaactcgc aagccgactg 
     6481 atgccttctg aacaatggaa aggcattatt gccgtaagcc gtggcggtct gtaccgggtg 
     6541 cgttactggc gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg 
     6601 atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct 
     6661 tcatcgttat tgatgacctg gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt 
     6721 atccaaaagc gcactttgtc accatcttcg caaaaccggc tggtcgtccg ctggttgatg 
     6781 actatgttgt tgatatcccg caagatacct ggattgaaca gccgtgggat atgggcgtcg 
     6841 tattcgtccc gccaatctcc ggtcgctaat cttttcaacg cctggcactg ccgggcgttg 
     6901 ttctttttaa cttcaggcgg gttacaatag tttccagtaa gtattctgga ggctgcatcc 
     6961 atgacacagg caaacctgag cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc 
     7021 tcgacgctag tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat 
     7081 ggtaaaacca tccctcactg gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc 
     7141 attgacccac gcgaaatcct cgacgtccag gcacgtattg tgatgagcga tgccgaacgt 
     7201 accgacgatg atttatacga tacggtgatt ggctaccgtg gcggcaactg gatttatgag 
     7261 tgggccccgg atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact 
     7321 acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa 
     7381 actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga 
     7441 gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag 
     7501 tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt 
     7561 agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag 
     7621 taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata 
     7681 caagaaaatt atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca 
     7741 taacatactg ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc 
     7801 tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat 
     7861 gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag gttttacttg 
     7921 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg 
     7981 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 
     8041 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 
     8101 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc 
     8161 ccaccccata ccctattacc actgccaatt acctagtggt ttcatttact ctaaacctgt 
     8221 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac 
     8281 ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg atctgtggat 
     8341 ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc caggggtcag 
     8401 atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga 
     8461 agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga 
     8521 tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt 
     8581 ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg 
     8641 actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc 
     8701 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc 
     8761 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa 
     8821 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga 
     8881 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga 
     8941 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc 
     9001 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg 
     9061 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc 
     9121 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat 
     9181 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc 
     9241 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga 
     9301 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat 
     9361 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac 
     9421 cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat gagggacaat 
     9481 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 
     9541 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 
     9601 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 
     9661 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 
     9721 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 
     9781 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 
     9841 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 
     9901 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 
     9961 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 
    10021 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 
    10081 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 
    10141 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 
    10201 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 
    10261 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact 
    10321 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata 
    10381 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttccg 
    10441 atcacgagac tagcctcgac aagcttgatc gatggctccg gtgcccgtca gtgggcagag 
    10501 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc 
    10561 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt 
    10621 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc 
    10681 aacgggtttg ccgccagaac acaggtgtcg tgacgcgcat ccaggcctaa gcttacgc 
//
