LOCUS       Ple155\(pEMS1424       18095 bp    DNA     circular     15-JAN-2010
SOURCE      
  ORGANISM  
COMMENT     PCR genotyping:
            Sense primer: oEMS2303 - 5' AGACTCACTCGTTAATTAGGTGCCC - 3' Tm=56.1 Deg C
            Antisense primer: oEMS2299 - 5' GAGAGGACGTCCCTGCCTTTT - 3 Tm=56.1 Deg C
            
            Cycling conditions:
            Step 1: 94 Deg C for 3 min
            Step 2: 94 Deg C for 1 min
            Step 3: 61 Deg C for 1 min
            Step 4: 72 Deg C for 45 sec
            Step 5: repeat Step 2-4 34 times
            Step 6: 72 Deg C for 5 min
            
            383 bp product
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|543239357|
COMMENT     VNTDBDATE|543239357|
COMMENT     LSOWNER|
COMMENT     VNTNAME|Ple155 (pEMS1424)|
COMMENT     VNTAUTHORNAME|Demo User|
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COMMENT                0 0) 
COMMENT          #139=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
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COMMENT          #140=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
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COMMENT          #142=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 
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COMMENT         (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 
COMMENT          (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 -365) 
COMMENT        1 1 0 1 1 
COMMENT        (mapper: 23.5132 -18.0184 40.0378 40.0378 0.01 10 -1 18095 18095 1 0 0) 
COMMENT        #149=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil -179 100) 
COMMENT              (CObjectList 
COMMENT               #150=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -883 100) 
COMMENT                     (CObjectList 
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COMMENT                            0.871165 0 -10 11.4392 1.47361 Nil) 
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COMMENT               #154=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -1232 100) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "I-Sce I" "@N" 1 
COMMENT                                   -11.7917 -6.96932 0 -10 3.02214 1.17389 #156#) 
COMMENT                             #158=(CScratch 
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COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.65159 1.9 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "MFRT (F5mutant)" "@N" 1 13.6554 6.96932 0 
COMMENT                                   -10 7.91752 1.17389 #158#) 
COMMENT                             #160=(CScratch 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Kozak consensus" "@N" 1 14.5547 5.22699 0 
COMMENT                                   -10 7.99245 1.17389 #160#) 
COMMENT                             #162=(CWideArrow 
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COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Tetracysteine residue" "@N" 1 16.2902 
COMMENT                                   1.74233 0 -10 10.0405 1.17389 #164#) 
COMMENT                             #166=(CScratch 
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COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.37528 1.9 
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COMMENT                                   12.873 0 0 -10 1.99811 1.17389 #166#) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 early polyA signal" "@N" 1 16.4073 
COMMENT                                   -3.48466 0 -10 11.0645 1.17389 #170#) 
COMMENT                             #172=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #13# 0) 1 2 0 0 #114# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 5.65644 
COMMENT                                    6.22916 1 0.082322) 0.8 1.8 0) 
COMMENT                             #173=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Ple155 (PCP2-B)" "@N" 1 8.14418 12.1963 0 
COMMENT                                   -10 7.49292 1.17389 #172#) 
COMMENT                             #174=(CWideArrow 
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COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Region Prom 2 (reverse complement)" "@N" 1 
COMMENT                                   10.6352 15.681 0 -10 17.3586 1.17389 #174#) 
COMMENT                             #176=(CWideArrow 
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COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                             #177=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "Region Prom 1" 
COMMENT                                   "@N" 1 11.777 8.71165 0 -10 6.96841 1.17389 
COMMENT                                   #176#)) (CObjectList)) 
COMMENT                      #178=(CGroupWidget 
COMMENT                            (CWidget 22 (7 22 0) 1 2 0 0 Nil -325 100) 
COMMENT                            (CObjectList 
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COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 1 
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COMMENT                             #180=(CLabel (CWidget 0 (0 0) 1 2 0 0 #110# 6 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                   -4.62354 12.1963 0 -10 2.87229 1.17389 #179#) 
COMMENT                             #181=(CWideArrow 
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COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 0.835356 
COMMENT                                    2.13659 2.63096 1 0.082322) 0.8 1.8 0) 
COMMENT                             #182=(CLabel (CWidget 0 (0 0) 1 2 0 0 #110# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "AmpR" "@N" 1 
COMMENT                                   -10.0312 -8.71165 0 -10 2.87229 1.17389 #181#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #183=(CGroupWidget 
COMMENT                            (CWidget 27 (7 27 0) 1 2 0 0 Nil -1093 100) 
COMMENT                            (CObjectList 
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COMMENT                                   (CWidget 0 (3 #14# 0) 1 2 0 0 #118# 718 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 6.04958 1.9 
COMMENT                                   0.082322 1) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "oEMS2303" "@N" 
COMMENT                                   1 5.80569 13.9386 0 -10 4.79547 1.17389 #184#) 
COMMENT                             #186=(CScratch 
COMMENT                                   (CWidget 0 (3 #15# 0) 1 2 0 0 #118# 1 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.92269 1.9 
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COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #110# 21874452 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "oEMS2299" "@N" 
COMMENT                                   1 8.62528 10.454 0 -10 4.79547 1.17389 #186#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #188=(CGroupWidget 
COMMENT                            (CWidget 86 (7 86 0) 1 2 0 0 Nil -674 100) 
COMMENT                            (CObjectList 
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COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                                    2.12793 1 0.082322) 0.8 1.8 0) 
COMMENT                             #190=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "5' homology sequence" "@N" 1 -16.5102 
COMMENT                                   1.74233 0 -10 10.4901 1.17389 #189#) 
COMMENT                             #191=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #3# 0) 1 2 0 0 #114# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 2.62923 3.89322 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #192=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #110# 7258160 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "3' homology sequence" "@N" 1 6.77753 
COMMENT                                   -12.1963 0 -10 10.4901 1.17389 #191#) 
COMMENT                             #193=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #4# 0) 1 2 0 0 #114# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 3.89322 
COMMENT                                    5.264 1 0.082322) 0.8 1.8 0) 
COMMENT                             #194=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "HPRT complementary sequence" "@N" 1 18.5845 
COMMENT                                   -5.22699 0 -10 14.9609 1.17389 #193#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #195=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -256 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #196=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -116 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #197=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 963 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) 
COMMENT        (CObList #198=(AnlzFontColorItem 645 669 41943168) 
COMMENT         #199=(AnlzFontColorItem 1011 1031 41943168)) 1 
COMMENT        (CObList #200=(AnlzFontBackColorItem 127 792 33619967) 
COMMENT         #201=(AnlzFontBackColorItem 793 1778 33554687))) (CObList) 0 
COMMENT       (CStringList) 0 1684625219 (CObList)))
FEATURES             Location/Qualifiers
     misc_recomb     11958..15769
                     /vntifkey="86"
                     /label=5'\homology\sequence
     misc_marker     complement(16868..18089)
                     /vntifkey="22"
                     /label=AmpR
     misc_marker     10507..11932
                     /vntifkey="22"
                     /label=AmpR
     misc_recomb     6866..10511
                     /vntifkey="86"
                     /label=3'\homology\sequence
                     /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3"
     misc_recomb     2912..6865
                     /vntifkey="86"
                     /label=HPRT\complementary\sequence
                     /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2"
     misc_feature    11939..11956
                     /vntifkey="21"
                     /label=I-Sce\I
     misc_feature    1794..1827
                     /vntifkey="21"
                     /label=MFRT\(F5mutant)
     misc_feature    1828..1838
                     /vntifkey="21"
                     /label=Kozak\consensus
     misc_feature    1835..2554
                     /vntifkey="21"
                     /label=EGFP
     misc_feature    2555..2590
                     /vntifkey="21"
                     /label=Tetracysteine\residue
     misc_feature    2591..2663
                     /vntifkey="21"
                     /label=NLS
     misc_feature    2669..2702
                     /vntifkey="21"
                     /label=MFRT
     misc_feature    2856..2906
                     /vntifkey="21"
                     /label=SV40\early\polyA\signal
     misc_feature    128..1779
                     /vntifkey="21"
                     /label=Ple155\(PCP2-B)
                     /note="1306"
     primer          646..670
                     /vntifkey="27"
                     /label=oEMS2303
     primer          1012..1032
                     /vntifkey="27"
                     /label=oEMS2299
     misc_feature    128..793
                     /vntifkey="21"
                     /label=Region\Prom\2\(reverse\complement)
     misc_feature    794..1779
                     /vntifkey="21"
                     /label=Region\Prom\1
BASE COUNT     4729 a      4159 c      4186 g      5021 t 
ORIGIN
        1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 
       61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 
      121 cgagctctca gcagattgaa gaagccctcc tggtctgggg agcccgcctg gggacagact 
      181 cgctcagtct ggttggccct tcagcttggg ggcccctccc caaacttcct agccagcttg 
      241 ctcacaccct gaccccgggg cctgccgtcc ccacttcctc agctctcacc atggtccccg 
      301 ccgatcctct ctgcagagct gttctgaatg agacatgagt ctccttccca atcccggccc 
      361 ccgccccagg ggccctggcc agcagtgcca cttcacgtgg taccgcttca agggacaggc 
      421 tccgatgcgt gtcccgccgt cctagattgg ggcctgatga gtgtggcctg ggagctggga 
      481 cacgaatcag ggaaacatgg cccaggagct acccccaggt cccagcatct cccatcaata 
      541 ggggtccaca cggagagccc tgccctctgc cctggggcct ggcactcaga cccccaagcc 
      601 caccagcccc tttctacagc cacaactggg tcagggggtc ctaggagact cactcgttaa 
      661 ttaggtgccc tacaaactaa ttagtcttgt caatcatggg ctctgagacc ttgagctggg 
      721 ggtggggtgg gggcagggcc ctctcacctc ggcacagggg cctgagcctt cctccgtctt 
      781 ctcctcctga tccggacact tcattggcat agagggagag agtgtgaact tggccctttg 
      841 tggaacagag gaggctcggg cagaggtggt gatagtgcag cccattcatt ctgagatgaa 
      901 acttccactg gtttccgtaa agacgtcttg gggagggaag ggaaggggat ggggacctcc 
      961 cagtggtatc ccctgcttgg gcactgaggg aaagccacag tggctcgggg taaaaggcag 
     1021 ggacatcctc tccccgcctg cctctgtccc cagggagtct cgcctcctgt tcccacctgg 
     1081 ggctagggtg atagaggaga ggagatagct caacctggca tttaggtggt gtgggaacag 
     1141 gagaccccag actttcttgt tttggggtct ggggcaggca accaggctcc agggacagtg 
     1201 agttgaagga agggtggctg ggagacccct tgacttgctg ccaaggagac agagctggag 
     1261 ctagggtggc gggtggtgtc tgaggcaggt gcagagaggg agggagggaa ggggcctttg 
     1321 actccaacct cctttttctt taccgactgc aggtggcagc tgcccttcca ggagccagtg 
     1381 ggggaacctg ggtggctggg tggggacacc tgcaagtcct ccctaagcca gctaccaccc 
     1441 tacactgttg gcctcccttc tccaactgtg gggacgctgc tcaggccttt tgtgacatca 
     1501 cacctgagag tccctggggt ccagtcattg ctgctgggca cagcgaggtc caagctcagg 
     1561 tcgccctgcc ccctacccac catgccagat ccagcatcgt tgtgggcaaa caattatctg 
     1621 gatgatcttt atggggctta agcttgggtg ggagcagatg gggcatgagc tggggatttg 
     1681 gggatggggg gaatccacac ccccacgtcc tggacgttta aaaggccctc tctggcactg 
     1741 ggccggggca gaggccagca gaaaagtgac tggagtccaa cgcgtggcgc gccgaagttc 
     1801 ctattcttca aaaggtatag gaacttccgc caccatggtg agcaagggcg aggagctgtt 
     1861 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag 
     1921 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg 
     1981 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt 
     2041 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat 
     2101 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac 
     2161 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat 
     2221 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca 
     2281 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg 
     2341 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat 
     2401 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag 
     2461 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg 
     2521 gatcactctc ggcatggacg agctgtacaa gttatttctg aactgctgcc cgggctgctg 
     2581 catggaaccg gatccaaaaa agaagagaaa ggtagatcca aaaaagaaga gaaaggtaga 
     2641 tccaaaaaag aagagaaagg taatataaga agttcctatt ctctagaaag tataggaact 
     2701 tcagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc 
     2761 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt 
     2821 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 
     2881 cacaaataaa gcattttttt cactgcgaat tctcgtaaaa ctcttcatgg cagtagttat 
     2941 tattctctct ctctctcttt ttcttttttc ttgagacagg atatttctct gttgcccagg 
     3001 ctggagtgca gtggcacagt cttggctcac tgcagcctgg acctcctggg ctcaagccat 
     3061 cctcccacct cagcctccca agtagctggg gctacaggca catggccacc aggcccagat 
     3121 aatttttcat ttttgtagag actgagtctc accatgttac ccaggtttat tattctcatt 
     3181 ttttagatga agagactgag gtccagagaa gctcaatgac ttgcctagtt ttacaaatct 
     3241 cctgccatca catacccctc agcgtcctta ataagaggga ggccaccaac tatgtgctgg 
     3301 gcactgtggt ggatgctgga gctatagggt tgagtatata agaaatggtg ttgctggagc 
     3361 aactgttgct tgcttacctg acctatctga gaattaatta gcaggggaac atatttttgt 
     3421 tttcagattc aatataagaa cttgtgtggg caaaaataaa gatcagtagt aataacagta 
     3481 gttcccattt gctgactgta ctgtcctaag tgcatatata tatacataca cacacgcata 
     3541 cctatactcc tctaatactc aaaatgatcc tgtttatgta ttgttaatat gctcatttta 
     3601 tttttaaatt tttatttatt tttattttta tttatttttg agacggagtc tcattctgtc 
     3661 gcggaggctg aagtgcagtg gtgcgatctc agctcagtgc gacctccgcc tcccgggttc 
     3721 aagtgattct cctgcctcag cctccggact agctgggatt acaggcgccc gcctccacgc 
     3781 ccagctaatt tttgtatttt tagtagagat ggggtttcgc catgttggcc aggctggtct 
     3841 cgtactcctg accttgagtg atccacctgc ctcggcctcc caaagtgctg ggattacagg 
     3901 catgagccac cgcgccgggc taatatgctc attttagtga ggcaaaaata gaggctcaga 
     3961 gtctgatttg tacaaaacta cagagcagtt aagtgtcctc tcagatgtgt accctgatct 
     4021 gggtgactct aggactctag gtctcaactg ttacaaccag ttaagggttt ggggaagcac 
     4081 tgggccaaga gtcaggaaaa tggaagccac aggtagtgca aggtcttggg aatgggacgt 
     4141 ctggtccaag gattcacgcg atgactggaa cccgaagagc cggggcccgg tttacggccg 
     4201 ccatgaagca acgcgcgccg gtaggtttgg gaatcaggga gccctctgaa taggagactg 
     4261 agttgggagg gaaaggggct tcgctggggg agcctcggct tcttctggga gaaaattccc 
     4321 acggctacct agtgagcctg caaactggta ggcgccggcg taggcgcgcg ggcggggccg 
     4381 ggggcggggc ctgcggggcg tggcggggcg ggcagagggc ggggcctgct tctcctcagc 
     4441 ttcaggcggc tgcgacgagc cctcaggcga acctctcggc tttcccgcgc ggcgccgcct 
     4501 cttgctgcgc ctccgcctcc tcctctgctc cgccaccggc ttcctcctcc tgagcagtca 
     4561 gcccgcgcgc cggccggctc cgttatggcg acccgcagcc ctggcgtcgt ggtgagcagc 
     4621 tcggcctgcc ggccctggcc ggttcaggcc cacgcggcag gtggcggccg ggccctgagg 
     4681 cgcgggatcg atccatgaca cccccccgcc ccccatatct cactataatt cactgtcaaa 
     4741 tggcttttat gatttggagt ttggggatac ttttaaagac agggtcttgg tagctcctag 
     4801 atggcttggg attcacttag taacccttgc tggcctaaaa ttcaggaact tctacctcat 
     4861 cttcccaagt actgggttag tagggatgtc cagcatattt agcttgcttt aatgattttg 
     4921 ctgtactttt ttctgtgtgt acaggtgcat gtatatttat aaaactaaca taatatattt 
     4981 tacccccaaa gtaccataac cctaattaat gctattaaat gttttgtatg ctgggctctg 
     5041 ggcatactaa tataaccaat attatacaat gcaaaacaat tttggaatat cttctgtaaa 
     5101 attatcttat tagtaagttg taaccatctc agacttctaa gtttgagttt gtaaaggatc 
     5161 agtttttttt ggtgacctac acacacacac acacacacac acacacacac acacacaggg 
     5221 gggtggattc tgagaattta gagaatttag atggttaata ccagttttta tttttattct 
     5281 gtgtgatatt tatttattaa gatttattta tttattacat gtaggtacac tgtatctgtc 
     5341 ttcagacaca ccagaagagg gcatcagatc tcgttacaga tggttgtgag tcaccatgtg 
     5401 gttgctggga tttgaactca ggacctctgg aagaacagtc agtgctctta accgttgagc 
     5461 catctcacca gccccgtgat attttattta acttactaga atatactgtg ttaaaacata 
     5521 ttttagggga ttggggagat ggcccattct gtaaagtgtc tgccatgtca tcttcaggac 
     5581 ctgcgtttgt aaccccagca tggagaagac agagacaggg agacccctgg gcttcctggc 
     5641 aagctagact agcaaatttg gttaactagg ttcaatgaga gactcagtct caaaacataa 
     5701 gaagtgaatt ttttagaccc aatttcaatc tctgaactcc atacgtatgc acatgtacct 
     5761 gcatataaat atatatacac acccataaac acatgcacac acaaaaaatg gttcaaaagt 
     5821 tcttaaaatg tataagagta gaaagtgtag agccgggagt ggtggcgcac gcctttaatc 
     5881 ccagcacttg ggagcagagg caggtggatt tctgagttcg aggccagcct ggtctacaga 
     5941 gtgagttcca ggacagccag agctatacag agaaatcctg cctcaaaaaa ccaaaaataa 
     6001 agtgtatata ctaatctttc atgcctaaaa tatttatttt tgccacattt ttcatacctc 
     6061 tttaatattt ttctttttct ctttgtgctt caaaggaaat tttgtgtcat gttttacttt 
     6121 tcattcttga ctctattcag cagtaagacg cagcatggct ttcttagaga agtataatct 
     6181 attacaaaac ggtaacattt aactgctcta cataccctct tgtagctaaa ttcctctgtt 
     6241 aaactaagac ttattatcta aacgtaatta aatgttgata tcttataatg ctccactttg 
     6301 aaacagctgt ctttcttttg cagattagcg atgatgaacc aggttatgac ctagatttgt 
     6361 tttgtatacc taatcattat gccgaggatt tggaaaaagt gtttattcct catggactga 
     6421 ttatggacag gttagtaaga cctcgattga agttattttt agagggtgtg tgtgtggtgt 
     6481 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtaaga gagagaggag aggcatgtgt 
     6541 gatatatatg tgaagtgtat tgcatgtaca tggaatccag agaaggacat taggtgtcct 
     6601 actctgttgc tctcttattc ccttgaatca gtgtctcttg ctgaacctgg ggcatacata 
     6661 ctgatttggc taggctgcct ggctagcaag acccagggat ctgcttgtct ctgcccccca 
     6721 gtgctgacgt tacaagcagg tgcaaccaca tctggttttt atatgggtac tgggggatct 
     6781 gaacttaggg cctgatgcgt gcccaagtgc tctcacccgc caagccacct ctccagccaa 
     6841 agggtttttt ttcttgtatt aattctaact tagcaactgt tgagtagttg atgtttgaat 
     6901 ataagctaag ccaacttatt agaataaatg ttttctattt tttacttgta tgtgtgagtg 
     6961 cctgcatgta tgtatgtgaa gcatatgtat tccaggatgt cagaattgtg atggattccc 
     7021 tggaactgga gttacagacc attgtgaacc actgtgtatg ggtgctggga actgaacttt 
     7081 aggtcctctg caaaaacagt aaggatctta actgataaac tatctttcta gccctaaggc 
     7141 tttctacttt taaagttcgt ttctctttct ctctctctct ctctctctct ctctctctct 
     7201 ccctctccct ccctcctcct cctcctcctc tttcccccct ctcgcctcct ctttctctct 
     7261 cccctccctc tctctctctc acacacacac acagacagag agaggagaga gaaaggaagc 
     7321 cttactaatt aattacctag ttttagacat aactagtggg aatccatagt aagtaaatca 
     7381 acattcttga agagaggtta gaagaattgg ctctcgttgt ccatcgaggc tgtataagat 
     7441 tattcttctg ttttctcagc ttaccataca tattttctac gtagacaata ccaattcaaa 
     7501 tacactgtac gtaaagtata tgcgcacaca cacacacaca cacacacaca cacacacaca 
     7561 agttacatat tgtcatatag tactaaaatt tatggacttt ttcatattaa tacatctaga 
     7621 gctatcatgt tttttatctt ttaaaaatta ctttacgggg ctacagagat gtctcagcag 
     7681 ttaggagcac tgactgctct tccagaggtc ctgagttcaa ttcccagcaa ccacatggtg 
     7741 gctcacaacc atctgtaatg ggatctgatc cactcttatg gtgtgtctga agacagtgac 
     7801 agtgtactca tatacatgaa ataaatcttt aaaaaattac tttaccatca gggtatatat 
     7861 atatatatat atgtgtgtgt gtgtgtgtgt gtgtatgaag tatttgctat ttgagagggt 
     7921 ctcactgttt agctcagtct ggcttggaac gttctgtata gcccagactg gcctcaactc 
     7981 agcagtcccc ctgaaaggct ctcatatgtg gagattacag gtatgtgcta ctgtgcccag 
     8041 ccctacatgt tactttgttt ttgaaatagg gtatcaaata gcttagactg gcttctaagt 
     8101 cagtgtgtag ctaaggatgg tctgagcttc tgaacctgct acttcaactc ctggtgtgcc 
     8161 atcatggcca gtttgcacag ttgtgagagt agaactcagg acctcctatg tgttactaga 
     8221 catgttctct accagctgag ctacatccca attcgcctac atttactttt attttttaaa 
     8281 agatttattt atgtatacag cactctgcct gcatgtacat ctgcatgcca gaagagggca 
     8341 ccagatctca tcatacatag ttgtgagcca ccatgtggtt gctggggatt gaactcaggg 
     8401 cctctggaag agcagtcagt gctcttaacc tctgagccat ctctgtagcc ccctacattt 
     8461 attttaaaga ttataggcta ttctattcat agatgtgcca taactgattt aaccagatct 
     8521 tcattatttt gtacttagta gagtatatta gagggaatga atcttaaata cacattttga 
     8581 cacttgctca gctttagaca agtaaaaatc actggtcaag gaaatggtgc ttagtggatg 
     8641 cttctgaact gtcaaattgt cctccaacaa gttacatcat tttatacttc caacgttatg 
     8701 tgagagttca tttttcttta ggctcttcta gtattttata tttgagaaat aaaatttaac 
     8761 caaaatttgc caatctagta agcttgtttt tatttgtatc tttaaatttt atgatagatg 
     8821 ttatagtgta ctctcctctc ccctcccctc ccctctcctc ctcagcagga tcttgttatg 
     8881 tagccctaag ggatctaata cttactctgt agtccaggct gtccttcagc tcatgagaca 
     8941 caccatcatg cttggcatgt ttcttgttct gaaaggtatc tggttttaat tttggttgag 
     9001 ctggtgtgtt ggtgtatatc tgtaatctca gcacaggcaa gtagagacaa ggattatgat 
     9061 tacagtccag cctgggctat ttagagggat tctctttaat ctttcctcat gccccaaaat 
     9121 cttacctttg gtatatgaaa aatagtctcc acttctgcaa aatattgctt tatgaagtaa 
     9181 gaattaattc ccttcataga gacaaggaat gtgtcctgta aaagtttaat gtgtaagaag 
     9241 tatttgttat aaaagataaa tattcagaat cttcttttta attcctgatt ttatttctat 
     9301 aggactgaaa gacttgctcg agatgtcatg aaggagatgg gaggccatca cattgtggcc 
     9361 ctctgtgtgc tcaagggggg ctataagttc tttgctgacc tgctggatta cattaaagca 
     9421 ctgaatagaa atagtgatag atccattcct atgactgtag attttatcag actgaagagc 
     9481 tactgtgtaa gtataattaa cttataatta aaaaaatagg gccattctag ttttatctat 
     9541 atttttttta aacttgtgca aactatgcta cataatcaac ttacttgatt atgtaatata 
     9601 tatagtaata gttggaatta ctattattac atatttattt ttctgtgctg gggtagaacc 
     9661 cagggaacta agtactgtaa taaccgagct tcattcatcc tagtcatttt taattttttt 
     9721 tttttttttt gttttttcga gacaaggttt ctctgtgtag ccctggctgt cctggaactc 
     9781 actctgtaga ccaggctggc ctcgaactca gaaatctgcc tgcctctgcc tcccagtgct 
     9841 gagattaatg acgtgcacca ccactgcctg gcattttttt aaagttttaa tttgagaccc 
     9901 tttctcacta acttgctcac actatcctcg aactcattac gtgggctggt ctagaaattt 
     9961 cagtcaactt gcctcagctt ctcaagtagc tgggattaca ggcaggtgct accacttctg 
    10021 gccatgatca ttgttgttgt tgctattatt attattatta tttcactgat acttcatatc 
    10081 acaacacaga atagtaactg tagttcatga ccaacaaaaa agttgtacag tttctctgtt 
    10141 gtcttatttt gatctggatt ttgatttgtt tcatggcact gggcaggcta atctcaaact 
    10201 gcatggctca aatagtcctt ctgtcttaat ctcccatgtt ctggaagtat aggtatcact 
    10261 acacctagct gtattttcat attttaaaaa aaacgcctaa gaattgctat tgaatgaggc 
    10321 ggagagatgg ctcagcagtc acaagtactt gtagaggact ggggtttggt tactagcacc 
    10381 tcaaagatgg cttacaatta tctgtagctc caattccaga ggatctgtgt ggtaaagctg 
    10441 catgtatatg ggcatgcagg taaaactgct aaacatataa aaatagctca gtcttaagag 
    10501 aattggtcga ctttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt 
    10561 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat 
    10621 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa 
    10681 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga 
    10741 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa 
    10801 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt 
    10861 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg 
    10921 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg 
    10981 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg 
    11041 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag 
    11101 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca 
    11161 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg 
    11221 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc 
    11281 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg 
    11341 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg 
    11401 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac 
    11461 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg 
    11521 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg 
    11581 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact 
    11641 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa 
    11701 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt 
    11761 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag 
    11821 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct 
    11881 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc aggtcgagta 
    11941 gggataacag ggtaatggat cctcgttggg cacctgcact catgtacgca ggcccacata 
    12001 tagatgtaca cacgcatatc attcaaaata gaaattattt atacaagtag tgcgttggag 
    12061 agctggctca ggagttgtta atgcttgcta cccttgcaaa ggacccaagt tcaatttcca 
    12121 gcacctgtgt tgggcatctc acaaccacat gtaaatctag tttcagggga tttcacccct 
    12181 cttctgaact cttcaggcac ttatacccac aaatggacat ctccacatag agacacatac 
    12241 ttttaaaaaa gtaatctagg gtctagagaa atggttatag accacggact gggatcagtt 
    12301 cccagaagcc acatcctgtg gctcacaatt gcctgtgaat caagttctag atctggtggc 
    12361 ctcgtctagc ctgcagtcat caacaaacac atgcacacat ccatacacac ataatttaaa 
    12421 agaaagcgaa agtaacctat aatgctaaac aaagcacatt ctcagtgtag actgcagtgg 
    12481 tgatgaacct tgaaaatata ttaatagaag ccagacacgt aagtcacaaa tggtataact 
    12541 caatttatat gaaattccca gagagtccaa agttaaaaaa aacaaaaagg cagattattg 
    12601 gttgtcagta gctacagaga tgggacattg aagatattaa taggtatagg gttgaggatt 
    12661 catggccttt ccctcttcca tgttagcatg tctattgttg ttaaccttat tcaactcatg 
    12721 ttagggcaat catattgatg agacttaatg agtatagctt gacattccta gaagacagag 
    12781 tctcacatca gactctctga tcctctgact cttagaatct ttcaacaccc tcttctgaaa 
    12841 tgatctctga gcctttggtt gtgactgaat tatgagaaag aattttatgt tgaatgaatt 
    12901 ttacttcagc atttttacaa aactgaagct ggggatggcg tttcatgcgt gcagtcccta 
    12961 ccatttgcct ataagcctgg aggaggcaga agtagaatta ggcttttcag ggacatcttc 
    13021 agctgcatca tgagcttgag gacaacttga gctacataag attcccatcc ccacccccac 
    13081 acacacacac acatctagta tgtacctagt atgcagtctt ttcatatgtt taaaatgtag 
    13141 aaaggtatat gtgaagtctc ccttttcccc acaacttagt ccctccaaag tattgcatca 
    13201 gtttccttgt acatgatttt atgcatatat aagatacact atttctctct ctctctctct 
    13261 ccttccctcc ttccctccaa cctctgggta tgagttgagg gttatgtatg tttcaagttg 
    13321 taataagaat tgccactcaa acatttcatc ccattttaag ttaagtttgt ctgattttcc 
    13381 actttagaag ggcagccatg atggctcatg cctataatcc catcctagca cttgtaagcc 
    13441 taatacataa gactggagga ttgtcatcag cttgaggcta gcctggtcta cctaatgagt 
    13501 tccagccagc caagagtaga atataagacc ctgtctccaa aaaatgtacc aaaggggctg 
    13561 gaggggtaac tcagtggtta agaacagtag ctgctcttcc agaggacctg ggttcaattc 
    13621 ccagcaccca catggcagct tgcaactatc tgtaactgtc cagctttggg atatccgaca 
    13681 ccattatacc aattacataa aatgaaaaaa ttgtaaaatg tgctaaaatg agtggggagg 
    13741 gggagcatgg tgtggttctg tgggcttggg attttgccat gaatccatga ctatatctca 
    13801 gcaatatgtg gattatttct ggaaacctgt ggggcatggg tgtatgggaa gaggggagag 
    13861 agagagagcc cgcatccagc cagagttcct gtgctctggg caggcggacg tgggaggact 
    13921 gctggacgat ttccacttgg ccctgggtgg gcatctggct gtgtgaagcc actgacccca 
    13981 ctcagcgggg ggtagataag gggcatttcc cataccaggt tctcagactc cagcattcca 
    14041 cgctggatac agcagaagag ctgagaacag aataggggcc ccagggagac actctcgtcc 
    14101 cagagataaa gaaggcagag ggagagagat tcctacgcag gcgagaggcc ttagtctggg 
    14161 ccttgatgaa cagagacggt ctatggtttt agagctttat tgtagaaagg cagggggaaa 
    14221 gagagaaggt agaaagagag agaccagcca tggccaagag gagagaaggg gggaaaggag 
    14281 agagagaaga aaggctagag agtaagaaag gttaaagaga cagaagtaaa agcttagagt 
    14341 aagagagaga gagagagcaa gagggagagt aagaaaagta aaagcttaag agagcgagga 
    14401 ggggccagcc cctctaatag tgggcttgtt atcttgttgt tgctaggtaa ctgtgaagcg 
    14461 tctagcctga aggccagaag cttgggacat tgtctatgtg actgaaagcc atgagcctct 
    14521 actgtggggg ctgtggaggt ggtaacttgg acaggagcca ggggttccag gagacatgag 
    14581 ggaatgcctt ctgtcccatg taggtaggaa ttaccaccac cggctttcac cgtagttcac 
    14641 tggagactgt ttgtacatag cccattgccc cacagaaacc aggcactcac tgtttcattt 
    14701 gcataagaat tagaaggctt tattttggct gggattggtg acttgcccca gaaattccag 
    14761 cttttgggaa gccgaatcct ggagaaccag gagttcaagg tcatcctcag gtacctcatt 
    14821 tgttcaagac cagcctgtgc taccatgaga ccttgtccct actcccctca accccaaaac 
    14881 acgctgtttt cattttatag gttgtactaa ccatggagga tggtcatttg cctttctctt 
    14941 gatttcagaa gcgtagcgcg ggctttgcac catgatctag tgtttccaga taggcttgcc 
    15001 tctccacagc caggcttatc ctgcttcagt cccatgtttg gcaagggcaa catatgactg 
    15061 aagaacttgc atagccttat ggtgtatcat gactcttagc cccagtttat aactgtctaa 
    15121 cagctggtgg cttttgtgaa aaaggaaagc atcatttgaa cttttaactt ttcagctgtt 
    15181 tgttttgact ttgcataaat actgagacta gctaggaaat gagcttttgt ttcatttgct 
    15241 aaaaatgatt tttttttcat gaggcaattt caagcaattt gaatttagca atctctactt 
    15301 gtctgaaaag aagttattaa ttaaaaaaaa acaaacaaac aggaacctat tatgctggct 
    15361 agtcacatga aagaatccaa atgaacacag tcaaggctga atcaacacat gaaattattt 
    15421 tctaattccc caaattacat ttatgctaat tagtatgtag taaaacattt taattgtttc 
    15481 caatgttaaa cattacagaa ataattttga tgttttccta ataacccagc ctttgatcat 
    15541 gtttgcattt atttgattgt tgtcagttta ttaaataatt aagtaagagc aacaagataa 
    15601 aatgacttca tgagagtcct tttctgtaac ttagaacgtc agtagtcata ggaactgcgg 
    15661 tcgttccctt acctttcaga agtaggctaa agagttgaac gcaaaggtgg tttttcgaat 
    15721 cttcctgatc cttaagcccc cactaggttt atattctata tttgatatcg gattcgaacc 
    15781 cctcgagctt aggatccgga acccttaata taactaagtt attaggtccc tcgaagaggt 
    15841 tcactagtac tggccattgc ggccgcttcg aggctgcctc gcgcgtttcg gtgatgacgg 
    15901 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc 
    15961 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc 
    16021 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag 
    16081 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga 
    16141 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 
    16201 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 
    16261 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 
    16321 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 
    16381 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 
    16441 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 
    16501 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 
    16561 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 
    16621 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 
    16681 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 
    16741 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 
    16801 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 
    16861 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 
    16921 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 
    16981 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 
    17041 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 
    17101 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 
    17161 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 
    17221 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 
    17281 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 
    17341 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 
    17401 gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 
    17461 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 
    17521 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 
    17581 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 
    17641 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 
    17701 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 
    17761 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 
    17821 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 
    17881 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 
    17941 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 
    18001 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 
    18061 ccgcgcacat ttccccgaaa agtgccacct gacgg 
//
