LOCUS       Ple106\(pEMS1370       18063 bp    DNA     circular     15-JAN-2010
SOURCE      
  ORGANISM  
COMMENT     PCR genotyping:
            Sense primer: oEMS2364 - 5' GCGTATCACGAGGCCCTTTC - 3' Tm=56.0 Deg C
            Antisense primer: oEMS2380 - 5' CCACTCGACGTCTTCCATTTGA - 3 Tm=56.4 Deg C
            
            Cycling conditions:
            Step 1: 94 Deg C for 3 min
            Step 2: 94 Deg C for 1 min
            Step 3: 61 Deg C for 1 min
            Step 4: 72 Deg C for 45 sec
            Step 5: repeat Step 2-4 34 times
            Step 6: 72 Deg C for 5 min
            
            1100 bp product
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|543236988|
COMMENT     VNTDBDATE|543236988|
COMMENT     LSOWNER|
COMMENT     VNTNAME|Ple106 (pEMS1370)|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "Ple106 (pEMS1370)" 0 18063 
COMMENT       (CDBMol 0 0 1 1 1 0 0 1 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) (CStringList) 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 18063 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #0=(CFSignal (CObList) "5' homology sequence" 86 0 0 11926 15737 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "11926..15737") 
COMMENT        #1=(CFSignal (CObList) "AmpR" 22 0 1 16836 18057 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(16836..18057)") 
COMMENT        #2=(CFSignal (CObList) "AmpR" 22 0 0 10475 11900 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "10475..11900") 
COMMENT        #3=(CFSignal (CObList) "3' homology sequence" 86 0 0 6834 10479 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "6834..10479") 
COMMENT        #4=(CFSignal (CObList) "HPRT complementary sequence" 86 0 0 2880 6833 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "2880..6833") 
COMMENT        #5=(CFSignal (CObList) "I-Sce I" 21 0 0 11907 11924 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "11907..11924") 
COMMENT        #6=(CFSignal (CObList) "MFRT (F5mutant)" 21 0 0 1762 1795 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "1762..1795") 
COMMENT        #7=(CFSignal (CObList) "Kozak consensus" 21 0 0 1796 1806 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "1796..1806") 
COMMENT        #8=(CFSignal (CObList) "EGFP" 21 0 0 1803 2522 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1803..2522") 
COMMENT        #9=(CFSignal (CObList) "Tetracysteine residue" 21 0 0 2523 2558 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "2523..2558") 
COMMENT        #10=(CFSignal (CObList) "NLS" 21 0 0 2559 2631 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2559..2631") 
COMMENT        #11=(CFSignal (CObList) "MFRT" 21 0 0 2637 2670 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2637..2670") 
COMMENT        #12=(CFSignal (CObList) "SV40 early polyA signal" 21 0 0 2824 2874 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2824..2874") 
COMMENT        #13=(CFSignal (CObList) "Ple106 (HAP1-D)" 21 0 0 139 1753 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "139..1753") 
COMMENT        #14=(CFSignal (CObList) "oEMS2364" 27 0 0 54 73 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #15=(CFSignal (CObList) "oEMS2380" 27 0 0 1127 1148 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #16=(CFSignal (CObList) "Prom Region" 21 0 0 865 1741 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #17=(CFSignal (CObList) "Region 4" 21 0 0 494 864 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #18=(CFSignal (CObList) "Region 3" 21 0 0 139 493 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #19=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #20=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) 
COMMENT                    "Ple106 (pEMS1370)" 2) 5 "" 0 4) 
COMMENT              #21=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA 'Ple106 (pEMS1370)'" 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Foreign object. Author: Demo User. Original author: Demo User" 
COMMENT                          1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 01/15/10 11:09AM" 1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 01/15/10 11:09AM" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 18063 bp" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #29=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 15-JAN-2010" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #32=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 4 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Demo User" 
COMMENT                          1))) "Author") 
COMMENT              #34=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 5 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Demo User" 
COMMENT                          1))) "Original Author") 
COMMENT              #36=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #37=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) 
COMMENT                   "Annotations") 
COMMENT              #38=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #39=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #40=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 139 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #41=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 139   End: 1753" 1) 
COMMENT                                   #42=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) "1306" 
COMMENT                                        1) 
COMMENT                                   #43=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #44=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   139..1753" 1))) "Ple106 (HAP1-D)") 
COMMENT                            #45=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 139 (3 #18# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #46=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 139   End: 493 " 1))) "Region 3") 
COMMENT                            #47=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 494 (3 #17# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 494   End: 864 " 1))) "Region 4") 
COMMENT                            #49=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 865 (3 #16# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 865   End: 1741" 1))) 
COMMENT                                 "Prom Region") 
COMMENT                            #51=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1762 (3 #6# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1762  End: 1795" 1) 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1762..1795" 1))) "MFRT (F5mutant)") 
COMMENT                            #55=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1796 (3 #7# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1796  End: 1806" 1) 
COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1796..1806" 1))) "Kozak consensus") 
COMMENT                            #59=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1803 (3 #8# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1803  End: 2522" 1) 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1803..2522" 1))) "EGFP") 
COMMENT                            #63=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2523 (3 #9# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2523  End: 2558" 1) 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2523..2558" 1))) 
COMMENT                                 "Tetracysteine residue") 
COMMENT                            #67=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2559 (3 #10# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2559  End: 2631" 1) 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2559..2631" 1))) "NLS") 
COMMENT                            #71=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2637 (3 #11# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2637  End: 2670" 1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2637..2670" 1))) "MFRT") 
COMMENT                            #75=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2824 (3 #12# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2824  End: 2874" 1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2824..2874" 1))) 
COMMENT                                 "SV40 early polyA signal") 
COMMENT                            #79=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 11907 (3 #5# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 11907  End: 11924" 1) 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   11907..11924" 1))) "I-Sce I"))) 
COMMENT                          "Misc. Feature (12 total)") 
COMMENT                     #83=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #84=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 10475 (3 #2# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 10475  End: 11900" 1) 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   10475..11900" 1))) "AmpR") 
COMMENT                            #88=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 16836 (3 #1# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 16836  End: 18057 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(16836..18057)" 1))) 
COMMENT                                 "AmpR"))) "Misc. Marker (2 total)") 
COMMENT                     #92=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 27 (7 27 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #93=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 54 (3 #14# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 54    End: 73  " 1))) "oEMS2364") 
COMMENT                            #95=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1127 (3 #15# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #96=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1127  End: 1148" 1))) "oEMS2380"))) 
COMMENT                          "Primer (2 total)") 
COMMENT                     #97=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 86 (7 86 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #98=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2880 (3 #4# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2880  End: 6833" 1) 
COMMENT                                   #100=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2" 
COMMENT                                         1) 
COMMENT                                   #101=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "Original Location Description:" 1) 
COMMENT                                   #102=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "   2880..6833" 1))) 
COMMENT                                 "HPRT complementary sequence") 
COMMENT                            #103=(CFolderPar 
COMMENT                                  (CGroupPar 
COMMENT                                   (CParagraph 6834 (3 #3# 0) 1 2 2 0 194) 
COMMENT                                   (CObjectList 
COMMENT                                    #104=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 6834  End: 10479" 1) 
COMMENT                                    #105=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Consists of part of Intron 2, all of Exon 3, and part of Intron 3" 
COMMENT                                          1) 
COMMENT                                    #106=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #107=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   6834..10479" 1))) 
COMMENT                                  "3' homology sequence") 
COMMENT                            #108=(CFolderPar 
COMMENT                                  (CGroupPar 
COMMENT                                   (CParagraph 11926 (3 #0# 0) 1 2 2 0 194) 
COMMENT                                   (CObjectList 
COMMENT                                    #109=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 11926  End: 15737" 1) 
COMMENT                                    #110=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #111=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   11926..15737" 1))) 
COMMENT                                  "5' homology sequence"))) 
COMMENT                          "Misc. Recombination (3 total)"))) "Feature Map")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #112=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 
COMMENT                "@N (@S)" 0) 
COMMENT          #113=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #114=(CWidgetStyle "Molecule Label 2" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #115=(CWidgetStyle "Molecule Label 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #116=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 
COMMENT                (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #117=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 
COMMENT                (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #118=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 
COMMENT                (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) 
COMMENT          #119=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #120=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #121=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #122=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #123=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #124=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #125=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 
COMMENT                1 65535 "@N (@H)" 0) 
COMMENT          #126=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #127=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
COMMENT          #128=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #129=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT                0 0) 
COMMENT          #130=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) 
COMMENT          #131=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 
COMMENT                (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) 
COMMENT          #132=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #133=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #134=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #135=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #136=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #137=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #138=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #139=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #140=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #141=(CWidgetStyle "Annotation" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                0 0) 
COMMENT          #142=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #143=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #144=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #145=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 65535 "@N" 0) 
COMMENT          #146=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 
COMMENT                "@N" 0) 
COMMENT          #147=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 3 1 
COMMENT                (LOGBRUSH 0 16777113 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 
COMMENT                (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #148=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 
COMMENT                1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #149=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 
COMMENT                1 1 "@N" 0) 
COMMENT          #150=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #151=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 
COMMENT                "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 
COMMENT         (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 
COMMENT          (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 -365) 
COMMENT        1 1 0 1 1 
COMMENT        (mapper: 23.5132 -19.7619 40.0378 40.0378 0.01 10 -1 18063 18063 1 0 0) 
COMMENT        #152=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil -179 100) 
COMMENT              (CObjectList 
COMMENT               #153=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -953 100) 
COMMENT                     (CObjectList 
COMMENT                      #154=(CAxis 
COMMENT                            (CWideLine 
COMMENT                             (CWidget 0 (0 0) 1 2 0 0 #118# 47465796 0) 
COMMENT                             (LOGPEN 0 0 10079436) 2 (LOGBRUSH 0 13434879 0) 1 
COMMENT                             6.27453 6.27253 1 0.0214037) 0.0347295) 
COMMENT                      #155=(CLabel 
COMMENT                            (CWidget 1001 (0 0) 1 2 0 0 #115# 47450244 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 58 22 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 
COMMENT                            1.74233 0.833333 0 "Ple106 (pEMS1370)" "@N" 1 0 
COMMENT                            0.871165 0 -10 11.4392 1.47361 Nil) 
COMMENT                      #156=(CLabel 
COMMENT                            (CWidget 1002 (0 0) 1 2 0 0 #114# 47470916 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 
COMMENT                            1.74233 0.555556 0 "18063 bp" "@L bp" 16 0 -0.871165 
COMMENT                            0 -10.8 3.19698 0.949103 Nil)) (CObjectList)) 
COMMENT               #157=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -1232 100) 
COMMENT                     (CObjectList 
COMMENT                      #158=(CGroupWidget 
COMMENT                            (CWidget 21 (7 21 0) 1 2 0 0 Nil -116 100) 
COMMENT                            (CObjectList 
COMMENT                             #159=(CScratch 
COMMENT                                   (CWidget 0 (3 #5# 0) 1 2 0 0 #121# 1 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 2.13829 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #160=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #113# 46444972 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "I-Sce I" "@N" 1 
COMMENT                                   -11.7674 -6.96932 0 -10 3.02214 1.17389 #159#) 
COMMENT                             #161=(CScratch 
COMMENT                                   (CWidget 0 (3 #6# 0) 1 2 0 0 #121# 828 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.6616 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #162=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #113# 46621084 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "MFRT (F5mutant)" "@N" 1 13.6554 6.96932 0 
COMMENT                                   -10 7.91752 1.17389 #161#) 
COMMENT                             #163=(CScratch 
COMMENT                                   (CWidget 0 (3 #7# 0) 1 2 0 0 #121# 4543561 
COMMENT                                    100) (LOGPEN 0 8 13395507) 10 1 5.64979 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #164=(CLabel (CWidget 0 (0 0) 1 2 0 0 #113# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Kozak consensus" "@N" 1 14.5547 5.22699 0 
COMMENT                                   -10 7.99245 1.17389 #163#) 
COMMENT                             #165=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #8# 0) 1 2 0 0 #117# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 5.39731 
COMMENT                                    5.64736 1 0.082322) 0.8 1.8 0) 
COMMENT                             #166=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #113# 21517476 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "EGFP" "@N" 1 
COMMENT                                   12.7572 3.48466 0 -10 2.67247 1.17389 #165#) 
COMMENT                             #167=(CScratch 
COMMENT                                   (CWidget 0 (3 #9# 0) 1 2 0 0 #121# 821 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.39731 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #168=(CLabel (CWidget 0 (0 0) 1 2 0 0 #113# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Tetracysteine residue" "@N" 1 16.2902 
COMMENT                                   1.74233 0 -10 10.0405 1.17389 #167#) 
COMMENT                             #169=(CScratch 
COMMENT                                   (CWidget 0 (3 #10# 0) 1 2 0 0 #121# 667 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.38481 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #170=(CLabel (CWidget 0 (0 0) 1 2 0 0 #113# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "NLS" "@N" 1 
COMMENT                                   12.873 0 0 -10 1.99811 1.17389 #169#) 
COMMENT                             #171=(CScratch 
COMMENT                                   (CWidget 0 (3 #11# 0) 1 2 0 0 #121# 30114504 
COMMENT                                    100) (LOGPEN 0 8 13395507) 10 1 5.35772 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #172=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #113# 4063824 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "MFRT" "@N" 1 
COMMENT                                   13.1018 -1.74233 0 -10 2.72243 1.17389 #171#) 
COMMENT                             #173=(CScratch 
COMMENT                                   (CWidget 0 (3 #12# 0) 1 2 0 0 #121# 4063688 
COMMENT                                    100) (LOGPEN 0 8 13395507) 10 1 5.29277 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #174=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #113# 46591836 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 early polyA signal" "@N" 1 16.4073 
COMMENT                                   -3.48466 0 -10 11.0645 1.17389 #173#) 
COMMENT                             #175=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #13# 0) 1 2 0 0 #117# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 5.66438 
COMMENT                                    6.22526 1 0.082322) 0.8 1.8 0) 
COMMENT                             #176=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #113# 46572788 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Ple106 (HAP1-D)" "@N" 1 8.14751 12.1963 0 
COMMENT                                   -10 7.49292 1.17389 #175#) 
COMMENT                             #177=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #16# 0) 1 2 0 0 #117# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.670712 5.66855 
COMMENT                                    5.97312 1 0.082322) 0.8 1.8 0) 
COMMENT                             #178=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #113# 21876868 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "Prom Region" 
COMMENT                                   "@N" 1 11.4198 8.71165 0 -10 6.16917 1.17389 
COMMENT                                   #177#) 
COMMENT                             #179=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #17# 0) 1 2 0 0 #117# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 5.97312 6.10197 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #180=(CLabel (CWidget 0 (0 0) 1 2 0 0 #113# 6 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "Region 4" "@N" 
COMMENT                                   1 5.64074 13.9386 0 -10 4.19603 1.17389 #179#) 
COMMENT                             #181=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #18# 0) 1 2 0 0 #117# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 6.10197 6.22526 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #182=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #113# 36830816 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "Region 3" "@N" 
COMMENT                                   1 4.25064 15.681 0 -10 4.19603 1.17389 #181#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #183=(CGroupWidget 
COMMENT                            (CWidget 22 (7 22 0) 1 2 0 0 Nil -325 100) 
COMMENT                            (CObjectList 
COMMENT                             #184=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #1# 0) 1 2 0 0 #129# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 1 
COMMENT                                    0.00208377 0.426478 1 0.082322) 0.8 1.8 1) 
COMMENT                             #185=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #113# 37249748 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "AmpR" "@N" 1 
COMMENT                                   -4.62798 12.1963 0 -10 2.87229 1.17389 #184#) 
COMMENT                             #186=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #2# 0) 1 2 0 0 #129# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 0.835356 
COMMENT                                    2.14038 2.63562 1 0.082322) 0.8 1.8 0) 
COMMENT                             #187=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #113# 4063824 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "AmpR" "@N" 1 
COMMENT                                   -10.0172 -8.71165 0 -10 2.87229 1.17389 #186#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #188=(CGroupWidget 
COMMENT                            (CWidget 27 (7 27 0) 1 2 0 0 Nil -1093 100) 
COMMENT                            (CObjectList 
COMMENT                             #189=(CScratch 
COMMENT                                   (CWidget 0 (3 #14# 0) 1 2 0 0 #121# 4063688 
COMMENT                                    100) (LOGPEN 0 8 13395507) 10 1 6.25478 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #190=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #113# -116 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "oEMS2364" "@N" 
COMMENT                                   1 3.5387 17.4233 0 -10 4.79547 1.17389 #189#) 
COMMENT                             #191=(CScratch 
COMMENT                                   (CWidget 0 (3 #15# 0) 1 2 0 0 #121# 1 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.88213 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #192=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #113# 4063648 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "oEMS2380" "@N" 
COMMENT                                   1 8.62528 10.454 0 -10 4.79547 1.17389 #191#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #193=(CGroupWidget 
COMMENT                            (CWidget 86 (7 86 0) 1 2 0 0 Nil -674 100) 
COMMENT                            (CObjectList 
COMMENT                             #194=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #0# 0) 1 2 0 0 #117# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 0.807808 
COMMENT                                    2.1317 1 0.082322) 0.8 1.8 0) 
COMMENT                             #195=(CLabel (CWidget 0 (0 0) 1 2 0 0 #113# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "5' homology sequence" "@N" 1 -16.5134 
COMMENT                                   1.74233 0 -10 10.4901 1.17389 #194#) 
COMMENT                             #196=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #3# 0) 1 2 0 0 #117# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 2.63388 3.90012 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #197=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #113# 7258160 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "3' homology sequence" "@N" 1 6.8462 -12.1963 
COMMENT                                   0 -10 10.4901 1.17389 #196#) 
COMMENT                             #198=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #4# 0) 1 2 0 0 #117# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 3.90012 
COMMENT                                    5.27332 1 0.082322) 0.8 1.8 0) 
COMMENT                             #199=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #113# 28427156 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "HPRT complementary sequence" "@N" 1 18.597 
COMMENT                                   -5.22699 0 -10 14.9609 1.17389 #198#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #200=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -255 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #201=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -186 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #202=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -395 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) 
COMMENT        (CObList #203=(AnlzFontColorItem 53 72 33554560) 
COMMENT         #204=(AnlzFontColorItem 1126 1147 33587328)) 1 
COMMENT        (CObList #205=(AnlzFontBackColorItem 864 1740 33619967) 
COMMENT         #206=(AnlzFontBackColorItem 493 863 33619712) 
COMMENT         #207=(AnlzFontBackColorItem 138 492 33554687))) (CObList) 0 
COMMENT       (CStringList) 0 1684625219 (CObList)))
FEATURES             Location/Qualifiers
     misc_recomb     11926..15737
                     /vntifkey="86"
                     /label=5'\homology\sequence
     misc_marker     complement(16836..18057)
                     /vntifkey="22"
                     /label=AmpR
     misc_marker     10475..11900
                     /vntifkey="22"
                     /label=AmpR
     misc_recomb     6834..10479
                     /vntifkey="86"
                     /label=3'\homology\sequence
                     /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3"
     misc_recomb     2880..6833
                     /vntifkey="86"
                     /label=HPRT\complementary\sequence
                     /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2"
     misc_feature    11907..11924
                     /vntifkey="21"
                     /label=I-Sce\I
     misc_feature    1762..1795
                     /vntifkey="21"
                     /label=MFRT\(F5mutant)
     misc_feature    1796..1806
                     /vntifkey="21"
                     /label=Kozak\consensus
     misc_feature    1803..2522
                     /vntifkey="21"
                     /label=EGFP
     misc_feature    2523..2558
                     /vntifkey="21"
                     /label=Tetracysteine\residue
     misc_feature    2559..2631
                     /vntifkey="21"
                     /label=NLS
     misc_feature    2637..2670
                     /vntifkey="21"
                     /label=MFRT
     misc_feature    2824..2874
                     /vntifkey="21"
                     /label=SV40\early\polyA\signal
     misc_feature    139..1753
                     /vntifkey="21"
                     /label=Ple106\(HAP1-D)
                     /note="1306"
     primer          54..73
                     /vntifkey="27"
                     /label=oEMS2364
     primer          1127..1148
                     /vntifkey="27"
                     /label=oEMS2380
     misc_feature    865..1741
                     /vntifkey="21"
                     /label=Prom\Region
     misc_feature    494..864
                     /vntifkey="21"
                     /label=Region\4
     misc_feature    139..493
                     /vntifkey="21"
                     /label=Region\3
BASE COUNT     4756 a      4141 c      4131 g      5035 t 
ORIGIN
        1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 
       61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 
      121 cgagctccta ggccggccat tatagagctc cctgggcgac agagcaagac tccatctcaa 
      181 aaaaaaaaaa aggtgggtct cctccaagcc tgtcttctct tatttggggc aggcaagagg 
      241 gagccatgag ctgccagaga ccaggcaccc agggctggga cagttcagag tcatgcacgg 
      301 ggcagtggct gtgcctggca tcgtcctagg cactgtgtgt ttatgaagat gagtgaggag 
      361 cagtgtttgt gcctgaggag ttcaagtgta gctggtgaga tgcaaacgca aagtggcaac 
      421 agctgaactc ctgctgttcc ctttacccca gaactgacct tccttcctgc tgggttctgc 
      481 aactccctga atgcgggcgg cagttacagt gagccaagat tgcactattg cactcctgcc 
      541 tggacgacag agattcggtc tcaaataaat aaataaataa accaacaaac ccaagtctga 
      601 ccacgtcatt actgtgccta aaactcccaa atagctttcc attggccaaa ggataaaccc 
      661 cacccccttg cccactactg tggtctggcc tgtgtgcact gttctgggcc ctccccgtcc 
      721 cagtgtctta tgatggcctc tgggcctttc cagtcagctt ggacccatgt ttttccttct 
      781 ttgcctgggt gagcttcact tgccctgtgg tcttagctta gtggttcctt tttccaggaa 
      841 tccttcctta ccttgcaggt cgggcacacg ccaccatgct tggctaattt ttgtattttt 
      901 agtagagaca gggtttcacc aggtggccag gcttgtctgg aactcctgac ctcaggtgat 
      961 tcgcctgccc cggcctccca aagtgctggg attacaggcg tgagccactg cgcccggcta 
     1021 cagggagact tttaagaggc gtctcccaag gagaacaggg gttcgccagc atggcaagag 
     1081 gagggcaaag cgaactaggg ttggaggtga agaacagtta atccaatcaa atggaagacg 
     1141 tcgagtggcg cgagcactgg ggtgtgagcg cgagggtcag ggttgcagcg ccaggcaggg 
     1201 ttgcagcgcc aggcagggtt gcagcgccag gcagggttgc agcgccaggc agggttgcag 
     1261 cacgttggaa gcgcactgga gtttggaatg tctctcccag tcaaccggga aagacgcagc 
     1321 gctgccctaa tgcactggcc accccagacc gtatgcacaa cgttgggttg gcagcttcta 
     1381 caccagggaa ggcacccgaa accccggcgc ggcagctggg gaggggctgc ccccagccag 
     1441 ggacccgcga gtgctggggc agccgcggct ttgggcagaa ccattctccg gagatggaga 
     1501 gggcagaaaa aatctttcgc tcccatccca actcggagcc atccggaact tgcactcgcc 
     1561 cttcttgccc tgcttttctt actgtgctct gttccgtcac cactagctcc cctcccgcgc 
     1621 ggctctgagt cccgcttgcc actagttgct tctcggcagc ccccacccct acgcgtggtc 
     1681 cggcccggcg gctggcgcag gttataaagg tcccgccgca gcagcggacg gcagactcga 
     1741 ggagctctat aatggcgcgc cgaagttcct attcttcaaa aggtatagga acttccgcca 
     1801 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg 
     1861 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct 
     1921 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca 
     1981 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga 
     2041 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct 
     2101 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc 
     2161 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc 
     2221 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga 
     2281 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg 
     2341 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc 
     2401 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg 
     2461 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt 
     2521 tatttctgaa ctgctgcccg ggctgctgca tggaaccgga tccaaaaaag aagagaaagg 
     2581 tagatccaaa aaagaagaga aaggtagatc caaaaaagaa gagaaaggta atataagaag 
     2641 ttcctattct ctagaaagta taggaacttc agcggccgcg actctagatc ataatcagcc 
     2701 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc 
     2761 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt 
     2821 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcgaattc 
     2881 tcgtaaaact cttcatggca gtagttatta ttctctctct ctctcttttt cttttttctt 
     2941 gagacaggat atttctctgt tgcccaggct ggagtgcagt ggcacagtct tggctcactg 
     3001 cagcctggac ctcctgggct caagccatcc tcccacctca gcctcccaag tagctggggc 
     3061 tacaggcaca tggccaccag gcccagataa tttttcattt ttgtagagac tgagtctcac 
     3121 catgttaccc aggtttatta ttctcatttt ttagatgaag agactgaggt ccagagaagc 
     3181 tcaatgactt gcctagtttt acaaatctcc tgccatcaca tacccctcag cgtccttaat 
     3241 aagagggagg ccaccaacta tgtgctgggc actgtggtgg atgctggagc tatagggttg 
     3301 agtatataag aaatggtgtt gctggagcaa ctgttgcttg cttacctgac ctatctgaga 
     3361 attaattagc aggggaacat atttttgttt tcagattcaa tataagaact tgtgtgggca 
     3421 aaaataaaga tcagtagtaa taacagtagt tcccatttgc tgactgtact gtcctaagtg 
     3481 catatatata tacatacaca cacgcatacc tatactcctc taatactcaa aatgatcctg 
     3541 tttatgtatt gttaatatgc tcattttatt tttaaatttt tatttatttt tatttttatt 
     3601 tatttttgag acggagtctc attctgtcgc ggaggctgaa gtgcagtggt gcgatctcag 
     3661 ctcagtgcga cctccgcctc ccgggttcaa gtgattctcc tgcctcagcc tccggactag 
     3721 ctgggattac aggcgcccgc ctccacgccc agctaatttt tgtattttta gtagagatgg 
     3781 ggtttcgcca tgttggccag gctggtctcg tactcctgac cttgagtgat ccacctgcct 
     3841 cggcctccca aagtgctggg attacaggca tgagccaccg cgccgggcta atatgctcat 
     3901 tttagtgagg caaaaataga ggctcagagt ctgatttgta caaaactaca gagcagttaa 
     3961 gtgtcctctc agatgtgtac cctgatctgg gtgactctag gactctaggt ctcaactgtt 
     4021 acaaccagtt aagggtttgg ggaagcactg ggccaagagt caggaaaatg gaagccacag 
     4081 gtagtgcaag gtcttgggaa tgggacgtct ggtccaagga ttcacgcgat gactggaacc 
     4141 cgaagagccg gggcccggtt tacggccgcc atgaagcaac gcgcgccggt aggtttggga 
     4201 atcagggagc cctctgaata ggagactgag ttgggaggga aaggggcttc gctgggggag 
     4261 cctcggcttc ttctgggaga aaattcccac ggctacctag tgagcctgca aactggtagg 
     4321 cgccggcgta ggcgcgcggg cggggccggg ggcggggcct gcggggcgtg gcggggcggg 
     4381 cagagggcgg ggcctgcttc tcctcagctt caggcggctg cgacgagccc tcaggcgaac 
     4441 ctctcggctt tcccgcgcgg cgccgcctct tgctgcgcct ccgcctcctc ctctgctccg 
     4501 ccaccggctt cctcctcctg agcagtcagc ccgcgcgccg gccggctccg ttatggcgac 
     4561 ccgcagccct ggcgtcgtgg tgagcagctc ggcctgccgg ccctggccgg ttcaggccca 
     4621 cgcggcaggt ggcggccggg ccctgaggcg cgggatcgat ccatgacacc cccccgcccc 
     4681 ccatatctca ctataattca ctgtcaaatg gcttttatga tttggagttt ggggatactt 
     4741 ttaaagacag ggtcttggta gctcctagat ggcttgggat tcacttagta acccttgctg 
     4801 gcctaaaatt caggaacttc tacctcatct tcccaagtac tgggttagta gggatgtcca 
     4861 gcatatttag cttgctttaa tgattttgct gtactttttt ctgtgtgtac aggtgcatgt 
     4921 atatttataa aactaacata atatatttta cccccaaagt accataaccc taattaatgc 
     4981 tattaaatgt tttgtatgct gggctctggg catactaata taaccaatat tatacaatgc 
     5041 aaaacaattt tggaatatct tctgtaaaat tatcttatta gtaagttgta accatctcag 
     5101 acttctaagt ttgagtttgt aaaggatcag ttttttttgg tgacctacac acacacacac 
     5161 acacacacac acacacacac acacaggggg gtggattctg agaatttaga gaatttagat 
     5221 ggttaatacc agtttttatt tttattctgt gtgatattta tttattaaga tttatttatt 
     5281 tattacatgt aggtacactg tatctgtctt cagacacacc agaagagggc atcagatctc 
     5341 gttacagatg gttgtgagtc accatgtggt tgctgggatt tgaactcagg acctctggaa 
     5401 gaacagtcag tgctcttaac cgttgagcca tctcaccagc cccgtgatat tttatttaac 
     5461 ttactagaat atactgtgtt aaaacatatt ttaggggatt ggggagatgg cccattctgt 
     5521 aaagtgtctg ccatgtcatc ttcaggacct gcgtttgtaa ccccagcatg gagaagacag 
     5581 agacagggag acccctgggc ttcctggcaa gctagactag caaatttggt taactaggtt 
     5641 caatgagaga ctcagtctca aaacataaga agtgaatttt ttagacccaa tttcaatctc 
     5701 tgaactccat acgtatgcac atgtacctgc atataaatat atatacacac ccataaacac 
     5761 atgcacacac aaaaaatggt tcaaaagttc ttaaaatgta taagagtaga aagtgtagag 
     5821 ccgggagtgg tggcgcacgc ctttaatccc agcacttggg agcagaggca ggtggatttc 
     5881 tgagttcgag gccagcctgg tctacagagt gagttccagg acagccagag ctatacagag 
     5941 aaatcctgcc tcaaaaaacc aaaaataaag tgtatatact aatctttcat gcctaaaata 
     6001 tttatttttg ccacattttt catacctctt taatattttt ctttttctct ttgtgcttca 
     6061 aaggaaattt tgtgtcatgt tttacttttc attcttgact ctattcagca gtaagacgca 
     6121 gcatggcttt cttagagaag tataatctat tacaaaacgg taacatttaa ctgctctaca 
     6181 taccctcttg tagctaaatt cctctgttaa actaagactt attatctaaa cgtaattaaa 
     6241 tgttgatatc ttataatgct ccactttgaa acagctgtct ttcttttgca gattagcgat 
     6301 gatgaaccag gttatgacct agatttgttt tgtataccta atcattatgc cgaggatttg 
     6361 gaaaaagtgt ttattcctca tggactgatt atggacaggt tagtaagacc tcgattgaag 
     6421 ttatttttag agggtgtgtg tgtggtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt 
     6481 gtgtaagaga gagaggagag gcatgtgtga tatatatgtg aagtgtattg catgtacatg 
     6541 gaatccagag aaggacatta ggtgtcctac tctgttgctc tcttattccc ttgaatcagt 
     6601 gtctcttgct gaacctgggg catacatact gatttggcta ggctgcctgg ctagcaagac 
     6661 ccagggatct gcttgtctct gccccccagt gctgacgtta caagcaggtg caaccacatc 
     6721 tggtttttat atgggtactg ggggatctga acttagggcc tgatgcgtgc ccaagtgctc 
     6781 tcacccgcca agccacctct ccagccaaag ggtttttttt cttgtattaa ttctaactta 
     6841 gcaactgttg agtagttgat gtttgaatat aagctaagcc aacttattag aataaatgtt 
     6901 ttctattttt tacttgtatg tgtgagtgcc tgcatgtatg tatgtgaagc atatgtattc 
     6961 caggatgtca gaattgtgat ggattccctg gaactggagt tacagaccat tgtgaaccac 
     7021 tgtgtatggg tgctgggaac tgaactttag gtcctctgca aaaacagtaa ggatcttaac 
     7081 tgataaacta tctttctagc cctaaggctt tctactttta aagttcgttt ctctttctct 
     7141 ctctctctct ctctctctct ctctctctcc ctctccctcc ctcctcctcc tcctcctctt 
     7201 tcccccctct cgcctcctct ttctctctcc cctccctctc tctctctcac acacacacac 
     7261 agacagagag aggagagaga aaggaagcct tactaattaa ttacctagtt ttagacataa 
     7321 ctagtgggaa tccatagtaa gtaaatcaac attcttgaag agaggttaga agaattggct 
     7381 ctcgttgtcc atcgaggctg tataagatta ttcttctgtt ttctcagctt accatacata 
     7441 ttttctacgt agacaatacc aattcaaata cactgtacgt aaagtatatg cgcacacaca 
     7501 cacacacaca cacacacaca cacacacaag ttacatattg tcatatagta ctaaaattta 
     7561 tggacttttt catattaata catctagagc tatcatgttt tttatctttt aaaaattact 
     7621 ttacggggct acagagatgt ctcagcagtt aggagcactg actgctcttc cagaggtcct 
     7681 gagttcaatt cccagcaacc acatggtggc tcacaaccat ctgtaatggg atctgatcca 
     7741 ctcttatggt gtgtctgaag acagtgacag tgtactcata tacatgaaat aaatctttaa 
     7801 aaaattactt taccatcagg gtatatatat atatatatat gtgtgtgtgt gtgtgtgtgt 
     7861 gtatgaagta tttgctattt gagagggtct cactgtttag ctcagtctgg cttggaacgt 
     7921 tctgtatagc ccagactggc ctcaactcag cagtccccct gaaaggctct catatgtgga 
     7981 gattacaggt atgtgctact gtgcccagcc ctacatgtta ctttgttttt gaaatagggt 
     8041 atcaaatagc ttagactggc ttctaagtca gtgtgtagct aaggatggtc tgagcttctg 
     8101 aacctgctac ttcaactcct ggtgtgccat catggccagt ttgcacagtt gtgagagtag 
     8161 aactcaggac ctcctatgtg ttactagaca tgttctctac cagctgagct acatcccaat 
     8221 tcgcctacat ttacttttat tttttaaaag atttatttat gtatacagca ctctgcctgc 
     8281 atgtacatct gcatgccaga agagggcacc agatctcatc atacatagtt gtgagccacc 
     8341 atgtggttgc tggggattga actcagggcc tctggaagag cagtcagtgc tcttaacctc 
     8401 tgagccatct ctgtagcccc ctacatttat tttaaagatt ataggctatt ctattcatag 
     8461 atgtgccata actgatttaa ccagatcttc attattttgt acttagtaga gtatattaga 
     8521 gggaatgaat cttaaataca cattttgaca cttgctcagc tttagacaag taaaaatcac 
     8581 tggtcaagga aatggtgctt agtggatgct tctgaactgt caaattgtcc tccaacaagt 
     8641 tacatcattt tatacttcca acgttatgtg agagttcatt tttctttagg ctcttctagt 
     8701 attttatatt tgagaaataa aatttaacca aaatttgcca atctagtaag cttgttttta 
     8761 tttgtatctt taaattttat gatagatgtt atagtgtact ctcctctccc ctcccctccc 
     8821 ctctcctcct cagcaggatc ttgttatgta gccctaaggg atctaatact tactctgtag 
     8881 tccaggctgt ccttcagctc atgagacaca ccatcatgct tggcatgttt cttgttctga 
     8941 aaggtatctg gttttaattt tggttgagct ggtgtgttgg tgtatatctg taatctcagc 
     9001 acaggcaagt agagacaagg attatgatta cagtccagcc tgggctattt agagggattc 
     9061 tctttaatct ttcctcatgc cccaaaatct tacctttggt atatgaaaaa tagtctccac 
     9121 ttctgcaaaa tattgcttta tgaagtaaga attaattccc ttcatagaga caaggaatgt 
     9181 gtcctgtaaa agtttaatgt gtaagaagta tttgttataa aagataaata ttcagaatct 
     9241 tctttttaat tcctgatttt atttctatag gactgaaaga cttgctcgag atgtcatgaa 
     9301 ggagatggga ggccatcaca ttgtggccct ctgtgtgctc aaggggggct ataagttctt 
     9361 tgctgacctg ctggattaca ttaaagcact gaatagaaat agtgatagat ccattcctat 
     9421 gactgtagat tttatcagac tgaagagcta ctgtgtaagt ataattaact tataattaaa 
     9481 aaaatagggc cattctagtt ttatctatat tttttttaaa cttgtgcaaa ctatgctaca 
     9541 taatcaactt acttgattat gtaatatata tagtaatagt tggaattact attattacat 
     9601 atttattttt ctgtgctggg gtagaaccca gggaactaag tactgtaata accgagcttc 
     9661 attcatccta gtcattttta attttttttt ttttttttgt tttttcgaga caaggtttct 
     9721 ctgtgtagcc ctggctgtcc tggaactcac tctgtagacc aggctggcct cgaactcaga 
     9781 aatctgcctg cctctgcctc ccagtgctga gattaatgac gtgcaccacc actgcctggc 
     9841 atttttttaa agttttaatt tgagaccctt tctcactaac ttgctcacac tatcctcgaa 
     9901 ctcattacgt gggctggtct agaaatttca gtcaacttgc ctcagcttct caagtagctg 
     9961 ggattacagg caggtgctac cacttctggc catgatcatt gttgttgttg ctattattat 
    10021 tattattatt tcactgatac ttcatatcac aacacagaat agtaactgta gttcatgacc 
    10081 aacaaaaaag ttgtacagtt tctctgttgt cttattttga tctggatttt gatttgtttc 
    10141 atggcactgg gcaggctaat ctcaaactgc atggctcaaa tagtccttct gtcttaatct 
    10201 cccatgttct ggaagtatag gtatcactac acctagctgt attttcatat tttaaaaaaa 
    10261 acgcctaaga attgctattg aatgaggcgg agagatggct cagcagtcac aagtacttgt 
    10321 agaggactgg ggtttggtta ctagcacctc aaagatggct tacaattatc tgtagctcca 
    10381 attccagagg atctgtgtgg taaagctgca tgtatatggg catgcaggta aaactgctaa 
    10441 acatataaaa atagctcagt cttaagagaa ttggtcgact ttttcgccct ttgacgttgg 
    10501 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct 
    10561 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg 
    10621 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg 
    10681 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc 
    10741 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag 
    10801 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg 
    10861 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt 
    10921 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt 
    10981 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt 
    11041 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa 
    11101 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag 
    11161 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac 
    11221 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac 
    11281 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac 
    11341 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac 
    11401 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact 
    11461 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg 
    11521 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt 
    11581 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat 
    11641 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta 
    11701 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa 
    11761 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 
    11821 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 
    11881 aaaaaaacca ccgctaccag gtcgagtagg gataacaggg taatggatcc tcgttgggca 
    11941 cctgcactca tgtacgcagg cccacatata gatgtacaca cgcatatcat tcaaaataga 
    12001 aattatttat acaagtagtg cgttggagag ctggctcagg agttgttaat gcttgctacc 
    12061 cttgcaaagg acccaagttc aatttccagc acctgtgttg ggcatctcac aaccacatgt 
    12121 aaatctagtt tcaggggatt tcacccctct tctgaactct tcaggcactt atacccacaa 
    12181 atggacatct ccacatagag acacatactt ttaaaaaagt aatctagggt ctagagaaat 
    12241 ggttatagac cacggactgg gatcagttcc cagaagccac atcctgtggc tcacaattgc 
    12301 ctgtgaatca agttctagat ctggtggcct cgtctagcct gcagtcatca acaaacacat 
    12361 gcacacatcc atacacacat aatttaaaag aaagcgaaag taacctataa tgctaaacaa 
    12421 agcacattct cagtgtagac tgcagtggtg atgaaccttg aaaatatatt aatagaagcc 
    12481 agacacgtaa gtcacaaatg gtataactca atttatatga aattcccaga gagtccaaag 
    12541 ttaaaaaaaa caaaaaggca gattattggt tgtcagtagc tacagagatg ggacattgaa 
    12601 gatattaata ggtatagggt tgaggattca tggcctttcc ctcttccatg ttagcatgtc 
    12661 tattgttgtt aaccttattc aactcatgtt agggcaatca tattgatgag acttaatgag 
    12721 tatagcttga cattcctaga agacagagtc tcacatcaga ctctctgatc ctctgactct 
    12781 tagaatcttt caacaccctc ttctgaaatg atctctgagc ctttggttgt gactgaatta 
    12841 tgagaaagaa ttttatgttg aatgaatttt acttcagcat ttttacaaaa ctgaagctgg 
    12901 ggatggcgtt tcatgcgtgc agtccctacc atttgcctat aagcctggag gaggcagaag 
    12961 tagaattagg cttttcaggg acatcttcag ctgcatcatg agcttgagga caacttgagc 
    13021 tacataagat tcccatcccc acccccacac acacacacac atctagtatg tacctagtat 
    13081 gcagtctttt catatgttta aaatgtagaa aggtatatgt gaagtctccc ttttccccac 
    13141 aacttagtcc ctccaaagta ttgcatcagt ttccttgtac atgattttat gcatatataa 
    13201 gatacactat ttctctctct ctctctctcc ttccctcctt ccctccaacc tctgggtatg 
    13261 agttgagggt tatgtatgtt tcaagttgta ataagaattg ccactcaaac atttcatccc 
    13321 attttaagtt aagtttgtct gattttccac tttagaaggg cagccatgat ggctcatgcc 
    13381 tataatccca tcctagcact tgtaagccta atacataaga ctggaggatt gtcatcagct 
    13441 tgaggctagc ctggtctacc taatgagttc cagccagcca agagtagaat ataagaccct 
    13501 gtctccaaaa aatgtaccaa aggggctgga ggggtaactc agtggttaag aacagtagct 
    13561 gctcttccag aggacctggg ttcaattccc agcacccaca tggcagcttg caactatctg 
    13621 taactgtcca gctttgggat atccgacacc attataccaa ttacataaaa tgaaaaaatt 
    13681 gtaaaatgtg ctaaaatgag tggggagggg gagcatggtg tggttctgtg ggcttgggat 
    13741 tttgccatga atccatgact atatctcagc aatatgtgga ttatttctgg aaacctgtgg 
    13801 ggcatgggtg tatgggaaga ggggagagag agagagcccg catccagcca gagttcctgt 
    13861 gctctgggca ggcggacgtg ggaggactgc tggacgattt ccacttggcc ctgggtgggc 
    13921 atctggctgt gtgaagccac tgaccccact cagcgggggg tagataaggg gcatttccca 
    13981 taccaggttc tcagactcca gcattccacg ctggatacag cagaagagct gagaacagaa 
    14041 taggggcccc agggagacac tctcgtccca gagataaaga aggcagaggg agagagattc 
    14101 ctacgcaggc gagaggcctt agtctgggcc ttgatgaaca gagacggtct atggttttag 
    14161 agctttattg tagaaaggca gggggaaaga gagaaggtag aaagagagag accagccatg 
    14221 gccaagagga gagaaggggg gaaaggagag agagaagaaa ggctagagag taagaaaggt 
    14281 taaagagaca gaagtaaaag cttagagtaa gagagagaga gagagcaaga gggagagtaa 
    14341 gaaaagtaaa agcttaagag agcgaggagg ggccagcccc tctaatagtg ggcttgttat 
    14401 cttgttgttg ctaggtaact gtgaagcgtc tagcctgaag gccagaagct tgggacattg 
    14461 tctatgtgac tgaaagccat gagcctctac tgtgggggct gtggaggtgg taacttggac 
    14521 aggagccagg ggttccagga gacatgaggg aatgccttct gtcccatgta ggtaggaatt 
    14581 accaccaccg gctttcaccg tagttcactg gagactgttt gtacatagcc cattgcccca 
    14641 cagaaaccag gcactcactg tttcatttgc ataagaatta gaaggcttta ttttggctgg 
    14701 gattggtgac ttgccccaga aattccagct tttgggaagc cgaatcctgg agaaccagga 
    14761 gttcaaggtc atcctcaggt acctcatttg ttcaagacca gcctgtgcta ccatgagacc 
    14821 ttgtccctac tcccctcaac cccaaaacac gctgttttca ttttataggt tgtactaacc 
    14881 atggaggatg gtcatttgcc tttctcttga tttcagaagc gtagcgcggg ctttgcacca 
    14941 tgatctagtg tttccagata ggcttgcctc tccacagcca ggcttatcct gcttcagtcc 
    15001 catgtttggc aagggcaaca tatgactgaa gaacttgcat agccttatgg tgtatcatga 
    15061 ctcttagccc cagtttataa ctgtctaaca gctggtggct tttgtgaaaa aggaaagcat 
    15121 catttgaact tttaactttt cagctgtttg ttttgacttt gcataaatac tgagactagc 
    15181 taggaaatga gcttttgttt catttgctaa aaatgatttt tttttcatga ggcaatttca 
    15241 agcaatttga atttagcaat ctctacttgt ctgaaaagaa gttattaatt aaaaaaaaac 
    15301 aaacaaacag gaacctatta tgctggctag tcacatgaaa gaatccaaat gaacacagtc 
    15361 aaggctgaat caacacatga aattattttc taattcccca aattacattt atgctaatta 
    15421 gtatgtagta aaacatttta attgtttcca atgttaaaca ttacagaaat aattttgatg 
    15481 ttttcctaat aacccagcct ttgatcatgt ttgcatttat ttgattgttg tcagtttatt 
    15541 aaataattaa gtaagagcaa caagataaaa tgacttcatg agagtccttt tctgtaactt 
    15601 agaacgtcag tagtcatagg aactgcggtc gttcccttac ctttcagaag taggctaaag 
    15661 agttgaacgc aaaggtggtt tttcgaatct tcctgatcct taagccccca ctaggtttat 
    15721 attctatatt tgatatcgga ttcgaacccc tcgagcttag gatccggaac ccttaatata 
    15781 actaagttat taggtccctc gaagaggttc actagtactg gccattgcgg ccgcttcgag 
    15841 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga 
    15901 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag 
    15961 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt 
    16021 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg 
    16081 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc 
    16141 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 
    16201 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 
    16261 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 
    16321 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 
    16381 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 
    16441 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 
    16501 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 
    16561 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 
    16621 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 
    16681 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 
    16741 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 
    16801 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 
    16861 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 
    16921 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 
    16981 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 
    17041 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 
    17101 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 
    17161 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 
    17221 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 
    17281 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 
    17341 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc 
    17401 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 
    17461 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 
    17521 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 
    17581 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 
    17641 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc 
    17701 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 
    17761 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 
    17821 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 
    17881 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 
    17941 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 
    18001 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 
    18061 cgg 
//
