LOCUS       pT2/HB_seq_verified        3558 bp    DNA     circular      6-MAR-2012
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|619881494|
COMMENT     VNTDBDATE|619881494|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pT2/HB_seq_verified|
COMMENT     VNTAUTHORNAME|Jason Bell|
COMMENT     VNTAUTHORTEL|612-624-4206|
COMMENT     VNTAUTHOREML|bellx059@umn.edu|
COMMENT     VNTAUTHORAD1|University of Minnesota|
COMMENT     VNTAUTHORAD2|5-208 MCB|
COMMENT     VNTAUTHORAD3|420 Washington Ave SE|
COMMENT     VNTAUTHORAD4|Minneapolis, MN 55455|
COMMENT     VNTOAUTHORNAME|Pery Hackett|
COMMENT     VNTOAUTHOREML|perry@biosci.cbs.umn.edu|
COMMENT     VNTOAUTHORWWW|http://www.cbs.umn.edu/labs/perry|
FEATURES             Location/Qualifiers
     CDS             531..758
                     /vntifkey="4"
                     /label=IR/DR(R)\Rmut13,Æ130,143,150
                     /note="IR/DR(R) has PCR induced mutations at bps 13 (T to A in the Ri), deleting bp 130 (ÆT), 143 (C to T) and 150 (C to A) [these bp designations correspond to the original defined IR/DR(R) from Ri to Ro, not the plasmid]"
     CDS             complement(119..345)
                     /vntifkey="4"
                     /label=IR/DR(L)\Lmut44
                     /note="IR/DR(L) appears to have a spontaneous mutation from A to T at bp 44 (happens to revert back to consensus) [the bp designation corresponds to the original defined IR/DR(L) from Lo to Li, not the plasmid]"
     promoter        74..94
                     /vntifkey="30"
                     /label=T7
     promoter        complement(818..837)
                     /vntifkey="30"
                     /label=T3
     promoter        complement(862..984)
                     /vntifkey="30"
                     /label=LacZ\promoter
     rep_origin      1078..1958
                     /vntifkey="33"
                     /label=ColE\Ori
     CDS             complement(2022..2879)
                     /vntifkey="4"
                     /label=Amp
     rep_origin      3010..3466
                     /vntifkey="33"
                     /label=F1\Ori
     modified_base   759..762
                     /vntifkey="23"
                     /label=TATA
     modified_base   115..118
                     /vntifkey="23"
                     /label=TATA
BASE COUNT      908 a       853 c       839 g       958 t 
ORIGIN
        1 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg 
       61 ccagtgagcg cgcgtaatac gactcactat agggcgaatt ggagctcgga tccctataca 
      121 gttgaagtcg gaagtttaca tacacttaag ttggagtcat taaaactcgt ttttcaacta 
      181 ctccacaaat ttcttgttaa caaacaatag ttttggcaag tcagttagga catctacttt 
      241 gtgcatgaca caagtcattt ttccaacaat tgtttacaga cagattattt cacttataat 
      301 tcactgtatc acaattccag tgggtcagaa gtttacatac actaagttga ctgtgccttt 
      361 aaacagcttg gaaaattcca gaaaatgatg tcatggcttt agaagcttga tatccatgga 
      421 attcactagt gcgcgcggcc gctctagatg gccagatcta gcttgtggaa ggctactcga 
      481 aatgtttgac ccaagttaaa caatttaaag gcaatgctac caaatactaa ttgagtgtat 
      541 gtaaacttct gacccactgg gaatgtgatg aaagaaataa aagctgaaat gaatcattct 
      601 ctctactatt attctgatat ttcacattct taaaataaag tggtgatcct aactgaccta 
      661 agacagggaa tttttactag gattaaatgt caggaattgt gaaaaagtga gtttaaatgt 
      721 atttggctaa ggtgtatgta aacttccgac ttcaactgta tagggatcct ctagctagag 
      781 tcgacctcga gggggggccc ggtacccagc ttttgttccc tttagtgagg gttaattgcg 
      841 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 
      901 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 
      961 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 
     1021 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 
     1081 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 
     1141 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 
     1201 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 
     1261 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 
     1321 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 
     1381 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 
     1441 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 
     1501 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 
     1561 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 
     1621 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 
     1681 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc 
     1741 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 
     1801 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 
     1861 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 
     1921 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 
     1981 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 
     2041 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 
     2101 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 
     2161 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 
     2221 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 
     2281 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 
     2341 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 
     2401 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 
     2461 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 
     2521 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 
     2581 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 
     2641 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 
     2701 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 
     2761 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 
     2821 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 
     2881 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 
     2941 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 
     3001 gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 
     3061 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 
     3121 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 
     3181 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 
     3241 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 
     3301 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 
     3361 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 
     3421 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttccat tcgccattca 
     3481 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg 
     3541 cgaaaggggg atgtgctg 
//
