LOCUS       TetO-FUW-Sox2           9373 bp    DNA     circular     28-JUL-2008
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|427827912|
COMMENT     VNTDBDATE|489062750|
COMMENT     LSOWNER|
COMMENT     VNTNAME|TetO-FUW-Sox2|
COMMENT     VNTAUTHORNAME|WernigLab|
COMMENT     VNTOAUTHORNAME|Ruth Foreman|
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COMMENT                            5 1 "GGTACC" "CCATGG") 
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FEATURES             Location/Qualifiers
     promoter        6997..7601
                     /dnas_title="CMV promoter/enhancer"
                     /vntifkey=29
                     /label=CMV promoter/enhancer
     LTR             7620..8294
                     /dnas_title="5' HIVR-U5"
                     /vntifkey=19
                     /label=5' HIVR-U5
     misc_binding    8318..9175
                     /dnas_title="HIV rev response element"
                     /vntifkey=20
                     /label=HIV rev response element
     misc_binding    9201..9378
                     /dnas_title="HIV flap/nuclear import signal"
                     /vntifkey=20
                     /label=HIV flap/nuclear import signal
     misc_feature    1477..2065
                     /dnas_title="WRE"
                     /vntifkey=21
                     /label=WRE
     LTR             2081..2776
                     /dnas_title="HIV delU3RdelU5"
                     /vntifkey=19
                     /label=HIV delU3RdelU5
     misc_marker     3956..4329
                     /dnas_title="ZEO"
                     /vntifkey=22
                     /label=ZEO
     misc_marker     5795..6653
                     /dnas_title="AMP"
                     /vntifkey=22
                     /label=AMP
     misc_feature    9381..9825
                     /dnas_title="TetO"
                     /vntifkey=21
                     /label=TetO
     misc_feature    1..1452
                     /note="Foxg1"
ORIGIN      
          1 aattcgatgc tggacatggg agataggaaa gaggtgaaaa tgattcccaa gtcctcgttc
         61 agcatcaaca gcctggtccc cgaggccgtc cagaacgaca accaccacgc gagccacggc
        121 caccacaaca gccaccaccc ccagcatcac catcatcatc accaccacca ccacccgccg
        181 ccgcccgcgc cccagccgcc tccaccgccg ccccagcagc agcagcagca gccgcccccc
        241 gccccgcagc ccccgcaggc gcgcggcgcc ccagcagccg acgacgacaa gggtccccag
        301 ccgctcctgc tcccgccctc caccgccctg gacggggcca aggctgacgc acttggagcc
        361 aaaggcgagc cgggcggcgg gccggcggag ctggcgcccg tcgggccgga cgagaaggag
        421 aagggcgcgg gcgccggggg ggaggagaag aaaggggcgg gcgagggcgg caaggacggg
        481 gaggggggca aggagggcga caagaagaac ggcaagtacg agaagccgcc gttcagctac
        541 aacgcgctca tcatgatggc catcaggcag agtcccgaga agcgcctgac gctcaatggc
        601 atctatgagt tcatcatgaa gaacttcccc tactaccgcg agaacaagca gggctggcag
        661 aactccatcc gccacaacct gtccctcaac aagtgcttcg tgaaggtacc gcgccactac
        721 gacgacccgg gcaagggcaa ctactggatg ctcgacccgt cgagcgacga cgtgttcatc
        781 ggcggcacga ccggcaagct gcggcgccgc tccaccacgt ctcgggccaa gctggccttt
        841 aagcgcgggg cgcgcctcac ctccaccggc ctcaccttca tggaccgcgc cggctccctc
        901 tactggccca tgtcgccctt cctgtccctg caccaccccc gcgccagcag cactttgagt
        961 tacaacggga ccacgtcggc ctaccccagc caccccatgc cctacagctc cgtgttgact
       1021 caaaactcgc tgggcaacaa ccactccttc tccaccgcca acgggctgag tgtggaccgg
       1081 ctggtcaacg gggagatccc gtacgccacg caccacctca cggccgctgc gctcgccgcc
       1141 tcggtgccct gcggcctgtc ggtgccctgc tccgggacct actccctcaa cccctgctcc
       1201 gtcaacctgc tcgcgggcca gaccagttac tttttccccc acgtcccgca cccgtcaatg
       1261 acttcgcaga ccagcacgtc catgagcgcc cgggccgcgt cctcctctac gtcgccgcag
       1321 gccccctcga ccctgccctg tgagtcttta agaccctctt tgccaagttt tacgacagga
       1381 ctgtccgggg gactgtctga ttatttcaca catcaaaatc aggggtcttc ttccaaccct
       1441 ttaatacatt aagaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt
       1501 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
       1561 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
       1621 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
       1681 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
       1741 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
       1801 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
       1861 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
       1921 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
       1981 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
       2041 atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagacct agaaaaacat
       2101 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
       2161 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
       2221 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
       2281 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
       2341 ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga
       2401 tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag
       2461 aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta
       2521 ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg
       2581 gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
       2641 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
       2701 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
       2761 atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
       2821 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
       2881 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
       2941 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
       3001 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
       3061 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       3121 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
       3181 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
       3241 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
       3301 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
       3361 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
       3421 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
       3481 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
       3541 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
       3601 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
       3661 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
       3721 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
       3781 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
       3841 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt
       3901 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg
       3961 ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt
       4021 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg
       4081 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca
       4141 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg
       4201 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt
       4261 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg
       4321 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg
       4381 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
       4441 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
       4501 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
       4561 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
       4621 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
       4681 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
       4741 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
       4801 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
       4861 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
       4921 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
       4981 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
       5041 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
       5101 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
       5161 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
       5221 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
       5281 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
       5341 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
       5401 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
       5461 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
       5521 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
       5581 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
       5641 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
       5701 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
       5761 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
       5821 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
       5881 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
       5941 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
       6001 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
       6061 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
       6121 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
       6181 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
       6241 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
       6301 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
       6361 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
       6421 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
       6481 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
       6541 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
       6601 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
       6661 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
       6721 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
       6781 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
       6841 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
       6901 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
       6961 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
       7021 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
       7081 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
       7141 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
       7201 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
       7261 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
       7321 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
       7381 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
       7441 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
       7501 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
       7561 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg
       7621 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
       7681 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
       7741 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
       7801 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
       7861 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
       7921 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
       7981 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
       8041 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
       8101 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
       8161 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
       8221 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
       8281 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
       8341 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
       8401 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
       8461 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
       8521 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
       8581 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
       8641 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
       8701 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
       8761 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
       8821 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
       8881 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
       8941 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
       9001 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
       9061 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
       9121 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac
       9181 tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg
       9241 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
       9301 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
       9361 agcagagatc cagtttggtt actcgagttt accactccct atcagtgata gagaaaagtg
       9421 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
       9481 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
       9541 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
       9601 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
       9661 tagagaaaag tgaaagtcga gctcggtacc cgggtcgagt aggcgtgtac ggtgggaggc
       9721 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       9781 ttttgacctc catagaagac accgggaccg atccagcctc cgcggccccg
//
