LOCUS       pCru5-ACC/ACC-mE        6983 bp    DNA     circular     17-OCT-2013
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|676156443|
COMMENT     VNTDBDATE|676156443|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCru5-ACC/ACC-mEGFP-IRES-mCherry|
COMMENT     VNTAUTHORNAME|Josh|
COMMENT     VNTOAUTHORNAME|Demo User|
FEATURES             Location/Qualifiers
     CDS             1393..2103
                     /vntifkey="4"
                     /label=mCherry
     LTR             5615..5691
                     /vntifkey="19"
                     /label=U5
                     /note="U5 region of MoMLV LTR"
     promoter        4948..5515
                     /vntifkey="29"
                     /label=P\CMV
                     /note="Human cytomegalovirus (CMV) immediate early promoter"
     misc_signal     5761..6595
                     /vntifkey="87"
                     /label=Psi
                     /note="Psi packaging signal"
     LTR             2676..2752
                     /vntifkey="19"
                     /label=U5
                     /note="U5 region of MoMLV LTR"
     LTR             2159..2607
                     /vntifkey="19"
                     /label=U3
                     /note="U3 region of MoMLV LTR"
     LTR             2608..2675
                     /vntifkey="19"
                     /label=R
                     /note="Repeat region"
     LTR             5547..5614
                     /vntifkey="19"
                     /label=R
                     /note="R region of MoMLV LTR"
     enhancer        2259..2432
                     /vntifkey="9"
                     /label=MoMLV\Enhancer
                     /note="ColE1 origin of replication"
     rep_origin      3092..3735
                     /vntifkey="33"
                     /label=ColE1\Ori
                     /note="ColE1 origin of replication"
     CDS             complement(3889..4746)
                     /vntifkey="4"
                     /label=AmpR
                     /note="Ampicillin resistance gene (beta-lactamase)"
     RBS             790..1365
                     /vntifkey="32"
                     /label=IRES
                     /note="Internal Ribosome Entry Site (IRES)"
     splicing_signal 5749..5753
                     /vntifkey="38"
                     /label=Splice\donor
                     /note="Splice donor"
     splicing_signal 6682..6692
                     /vntifkey="38"
                     /label=Splice\acceptor
                     /note="splice acceptro"
     CDS             34..762
                     /vntifkey="4"
                     /label=mEGFP
     misc_feature    17..39
                     /vntifkey="21"
                     /label=TIS:\\ACC/ACC
BASE COUNT     1647 a      1985 c      1777 g      1574 t 
ORIGIN
        1 tcgagtaact aactaaacca tgggttgatt accatgggcg aattcagcaa gggcgaggag 
       61 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag 
      121 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc 
      181 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac 
      241 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc 
      301 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac 
      361 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag 
      421 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac 
      481 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag 
      541 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc 
      601 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag 
      661 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc 
      721 gccgggatca ctctcggcat ggacgagctg tacaagttcg aataagcatg catctagggc 
      781 ggccaattcc gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa 
      841 taaggccggt gtgcgtttgt ctatatgtga ttttccacca tattgccgtc ttttggcaat 
      901 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct 
      961 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct 
     1021 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc 
     1081 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa 
     1141 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc 
     1201 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg 
     1261 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc 
     1321 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataagcttg ccacaacccg 
     1381 ggatccgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc 
     1441 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc 
     1501 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt 
     1561 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc 
     1621 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc 
     1681 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc 
     1741 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc 
     1801 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac 
     1861 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc 
     1921 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc 
     1981 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg 
     2041 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag 
     2101 taagcggccg ctcgacgata aaataaaaga ttttatttag tctccagaaa aaggggggaa 
     2161 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat 
     2221 ggaaaaatac ataactgaga atagagaagt tcagatcaag gtcaggaaca gatggaacag 
     2281 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa 
     2341 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc 
     2401 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag 
     2461 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg 
     2521 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat 
     2581 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg 
     2641 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct 
     2701 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttccgactt 
     2761 gtggtctcgc tgccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc 
     2821 ttcacatgca gcatgtatca aaattaattt ggtttttttt cttaagtatt tacattaaat 
     2881 ggccatagtt gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattggcg 
     2941 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 
     3001 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 
     3061 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 
     3121 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 
     3181 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 
     3241 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 
     3301 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 
     3361 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 
     3421 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 
     3481 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 
     3541 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 
     3601 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 
     3661 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 
     3721 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 
     3781 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 
     3841 gcgcaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 
     3901 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 
     3961 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 
     4021 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 
     4081 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 
     4141 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 
     4201 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 
     4261 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 
     4321 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 
     4381 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 
     4441 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 
     4501 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 
     4561 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 
     4621 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 
     4681 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 
     4741 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 
     4801 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 
     4861 ccgaaaagtg ccaccagctt tgctcttagg agtttcctaa tacatcccaa actcaaatat 
     4921 ataaagcatt tgacttgttc tatgccctag ttattaatag taatcaatta cggggtcatt 
     4981 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg 
     5041 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac 
     5101 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt 
     5161 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa 
     5221 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta 
     5281 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg 
     5341 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg 
     5401 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc 
     5461 attgacgcaa atgggcggta ggcatgtacg gtgggaggtc tatataagca gagctcaata 
     5521 aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt 
     5581 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt 
     5641 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat ttgggggctc 
     5701 gtccgggatc gggagacccc tgcccaggga ccaccgaccc accaccggga ggtaagctgg 
     5761 ccagcaactt atctgtgtct gtccgattgt ctagtgtcta tgactgattt tatgcgcctg 
     5821 cgtcggtact agttagctaa ctagctctgt atctggcgga cccgtggtgg aactgacgag 
     5881 ttcggaacac ccggccgcaa ccctgggaga cgtcccaggg acttcggggg ccgtttttgt 
     5941 ggcccgacct gagtccaaaa atcccgatcg ttttggactc tttggtgcac cccccttaga 
     6001 ggagggatat gtggttctgg taggagacga gaacctaaaa cagttcccgc ctccgtctga 
     6061 atttttgctt tcggtttggg accgaagccg cgccgcgcgt cttgtctgct gcagcatcgt 
     6121 tctgtgttgt ctctgtctga ctgtgtttct gtatttgtct gaaaatatcg gcccgggcca 
     6181 gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg agcggatcgc 
     6241 tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct ctgcagaatg 
     6301 gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc tcatcaccca 
     6361 ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg tcccctacat 
     6421 cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct ttgtacaccc 
     6481 taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac ctcctcgttc 
     6541 gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg cccccatatg 
     6601 gccatatgag atcttatatg gggcaccccc gccccttgta aacttccctg accctgacat 
     6661 gacaagagtt actaacagcc cctctctcca agctcactta caggctctct acttagtcca 
     6721 gcacgaagtc tggagacctc tggcggcagc ctaccaagaa caactggacc gaccggtggt 
     6781 acctcaccct taccgagtcg gcgacacagt gtgggtccgc cgacaccaga ctaagaacct 
     6841 agaacctcgc tggaaaggac cttacacagt cctgctgacc acccccaccg ccctcaaagt 
     6901 agacggcatc gcagcttgga tacacgccgc ccacgtgaag gctgccgacc ccgggggtgg 
     6961 accatcctct agactgccgg atc 
//
