LOCUS       Ple220\(pEMS1183       17439 bp    DNA     circular      4-FEB-2010
SOURCE      
  ORGANISM  
COMMENT     PCR genotyping:
            Sense primer: oEMS2626 - 5' CCCTGTACCCCTACCCTGACAA - 3' Tm=56.6 Deg C
            Antisense primer: oEMS2622 - 5' GACAGTAGCCACACACCCAGACA -3'  Tm=56.3 Deg C
            
            Cycling conditions:
            Step 1: 94 Deg C for 3 min
            Step 2: 94 Deg C for 1 min
            Step 3: 61 Deg C for 1 min
            Step 4: 72 Deg C for 45 sec
            Step 5: repeat Step 2-4 34 times
            Step 6: 72 Deg C for 5 min
            
            373 bp product
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|543238410|
COMMENT     VNTDBDATE|545051883|
COMMENT     LSOWNER|
COMMENT     VNTNAME|Ple220 (pEMS1183)|
COMMENT     VNTAUTHORNAME|Russell Bonaguro|
COMMENT     VNTOAUTHORNAME|Demo User|
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COMMENT          #140=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
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COMMENT         (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 
COMMENT          (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 -365) 
COMMENT        1 1 0 1 1 
COMMENT        (mapper: 23.5632 -19.7668 40.0378 40.0378 0.01 10 -1 17439 17439 1 0 0) 
COMMENT        #149=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil -1093 100) 
COMMENT              (CObjectList 
COMMENT               #150=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -1302 100) 
COMMENT                     (CObjectList 
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COMMENT                            0.871165 0 -10 11.4392 1.47361 Nil) 
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COMMENT               #154=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -814 100) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "I-Sce I" "@N" 1 
COMMENT                                   -11.4078 -6.96932 0 -10 3.02214 1.17389 #156#) 
COMMENT                             #158=(CScratch 
COMMENT                                   (CWidget 0 (3 #6# 0) 1 2 0 0 #118# 820 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.86418 1.9 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "MFRT (F5mutant)" "@N" 1 12.2822 8.71165 0 
COMMENT                                   -10 7.91752 1.17389 #158#) 
COMMENT                             #160=(CScratch 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Kozak consensus" "@N" 1 13.6554 6.96932 0 
COMMENT                                   -10 7.99245 1.17389 #160#) 
COMMENT                             #162=(CWideArrow 
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COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                                    5.84943 1 0.082322) 0.8 1.8 0) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                             #164=(CScratch 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Tetracysteine residue" "@N" 1 15.9718 
COMMENT                                   3.48466 0 -10 10.0405 1.17389 #164#) 
COMMENT                             #166=(CScratch 
COMMENT                                   (CWidget 0 (3 #10# 0) 1 2 0 0 #118# 31592976 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "NLS" "@N" 1 
COMMENT                                   12.7707 1.74233 0 -10 1.99811 1.17389 #166#) 
COMMENT                             #168=(CScratch 
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COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.54943 1.9 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "MFRT" "@N" 1 
COMMENT                                   13.2041 0 0 -10 2.72243 1.17389 #168#) 
COMMENT                             #170=(CScratch 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 early polyA signal" "@N" 1 16.7258 
COMMENT                                   -1.74233 0 -10 11.0645 1.17389 #170#) 
COMMENT                             #172=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #13# 0) 1 2 0 0 #114# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 5.86706 
COMMENT                                    6.22354 1 0.082322) 0.8 1.8 0) 
COMMENT                             #173=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #110# 47569932 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Ple220 (TAC3-C)" "@N" 1 7.08381 13.9386 0 
COMMENT                                   -10 7.41799 1.17389 #172#) 
COMMENT                             #174=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #16# 0) 1 2 0 0 #114# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                                    6.00375 1 0.082322) 0.8 1.8 0) 
COMMENT                             #175=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "Prom Region" 
COMMENT                                   "@N" 1 9.19154 10.454 0 -10 6.16917 1.17389 
COMMENT                                   #174#) 
COMMENT                             #176=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #17# 0) 1 2 0 0 #114# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                             #177=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Region 4 (reverse complement)" "@N" 1 
COMMENT                                   9.47916 17.4233 0 -10 14.5862 1.17389 #176#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #178=(CGroupWidget 
COMMENT                            (CWidget 22 (7 22 0) 1 2 0 0 Nil -116 100) 
COMMENT                            (CObjectList 
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COMMENT                                    (CWidget 0 (3 #1# 0) 1 2 0 0 #126# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 1 
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COMMENT                             #180=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                   -4.71763 12.1963 0 -10 2.87229 1.17389 #179#) 
COMMENT                             #181=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #2# 0) 1 2 0 0 #126# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 0.835356 
COMMENT                                    2.21696 2.72993 1 0.082322) 0.8 1.8 0) 
COMMENT                             #182=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "AmpR" "@N" 1 
COMMENT                                   -10.0172 -8.71165 0 -10 2.87229 1.17389 #181#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #183=(CGroupWidget 
COMMENT                            (CWidget 27 (7 27 0) 1 2 0 0 Nil -326 100) 
COMMENT                            (CObjectList 
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COMMENT                                    100) (LOGPEN 0 8 13395507) 10 1 6.09908 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #185=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #110# 35201548 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "oEMS2626" "@N" 
COMMENT                                   1 5.31937 15.681 0 -10 4.79547 1.17389 #184#) 
COMMENT                             #186=(CScratch 
COMMENT                                   (CWidget 0 (3 #15# 0) 1 2 0 0 #118# 1 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.97318 1.9 
COMMENT                                   0.082322 1) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "oEMS2622" "@N" 
COMMENT                                   1 6.57111 12.1963 0 -10 4.79547 1.17389 #186#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #188=(CGroupWidget 
COMMENT                            (CWidget 86 (7 86 0) 1 2 0 0 Nil -256 100) 
COMMENT                            (CObjectList 
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COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                                    2.20797 1 0.082322) 0.8 1.8 0) 
COMMENT                             #190=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "5' homology sequence" "@N" 1 -16.5493 0 0 
COMMENT                                   -10 10.4901 1.17389 #189#) 
COMMENT                             #191=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #3# 0) 1 2 0 0 #114# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 2.72813 4.03967 1 
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COMMENT                             #192=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #110# 47436280 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "3' homology sequence" "@N" 1 8.22178 
COMMENT                                   -12.1963 0 -10 10.4901 1.17389 #191#) 
COMMENT                             #193=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #4# 0) 1 2 0 0 #114# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 4.03967 
COMMENT                                    5.46201 1 0.082322) 0.8 1.8 0) 
COMMENT                             #194=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "HPRT complementary sequence" "@N" 1 18.5719 
COMMENT                                   -3.48466 0 -10 14.9609 1.17389 #193#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #195=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -326 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #196=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -186 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #197=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -814 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) 
COMMENT        (CObList #198=(AnlzFontColorItem 484 505 41943168) 
COMMENT         #199=(AnlzFontColorItem 834 856 41943168)) 1 
COMMENT        (CObList #200=(AnlzFontBackColorItem 749 1128 33554687) 
COMMENT         #201=(AnlzFontBackColorItem 138 748 33619967))) (CObList) 0 
COMMENT       (CStringList) 0 1684625219 (CObList)))
FEATURES             Location/Qualifiers
     misc_recomb     11302..15113
                     /vntifkey="86"
                     /label=5'\homology\sequence
     misc_marker     complement(16212..17433)
                     /vntifkey="22"
                     /label=AmpR
     misc_marker     9851..11276
                     /vntifkey="22"
                     /label=AmpR
     misc_recomb     6210..9855
                     /vntifkey="86"
                     /label=3'\homology\sequence
                     /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3"
     misc_recomb     2256..6209
                     /vntifkey="86"
                     /label=HPRT\complementary\sequence
                     /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2"
     misc_feature    11283..11300
                     /vntifkey="21"
                     /label=I-Sce\I
     misc_feature    1138..1171
                     /vntifkey="21"
                     /label=MFRT\(F5mutant)
     misc_feature    1172..1182
                     /vntifkey="21"
                     /label=Kozak\consensus
     misc_feature    1179..1898
                     /vntifkey="21"
                     /label=EGFP
     misc_feature    1899..1934
                     /vntifkey="21"
                     /label=Tetracysteine\residue
     misc_feature    1935..2007
                     /vntifkey="21"
                     /label=NLS
     misc_feature    2013..2046
                     /vntifkey="21"
                     /label=MFRT
     misc_feature    2200..2250
                     /vntifkey="21"
                     /label=SV40\early\polyA\signal
     misc_feature    139..1129
                     /vntifkey="21"
                     /label=Ple220\(TAC3-C)
                     /note="1306"
     primer          485..506
                     /vntifkey="27"
                     /label=oEMS2626
     primer          835..857
                     /vntifkey="27"
                     /label=oEMS2622
     misc_feature    750..1129
                     /vntifkey="21"
                     /label=Prom\Region
     misc_feature    139..749
                     /vntifkey="21"
                     /label=Region\4\(reverse\complement)
BASE COUNT     4646 a      3949 c      3900 g      4944 t 
ORIGIN
        1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 
       61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 
      121 cgagctccta ggccggccgt ggctccaaac ggtgtccagc ccatacttgg cactcccctg 
      181 ggtgtttttt ttttttaacg gttctggcct tttggatatg gcacaactgc tgccacctgc 
      241 aaggctgccc tggtgataac tcccttggcc cagcatgtgg tgttcaggca gccacttttc 
      301 ccaaattgtt gaatactctg cccacctcct ataatgagat gctgaacaga tttgagctga 
      361 agaggcagtt aaacttcaca cattgaattt gtaataagtc ccacccgcaa tcatcttaaa 
      421 agcatttgta ccgatgtaat tgctgtcatt tcaaaacctt gaaaagactt cctccctatc 
      481 ctccccctgt acccctaccc tgacaattcc tattcttttt cgtaaaggaa ctgtggaaat 
      541 cgaggctcaa tctggttttt ttgctgttgt tgttttccta tcctgactaa gcagaggata 
      601 gaggcgggca agggagaaaa gagaagaagg aaggggttgg gatcttagaa gcacctgagg 
      661 ttactggggg tggatgagca aaagataagg gaatttattt gcattttgaa aataaaacaa 
      721 tacacataac tgataggtaa aatagcacat caaactgtac acaaaccata cacagcagcc 
      781 agctcgggtg ctgttaggaa atggtctcac tgctgggtct gtggggtatg tgtgtgtctg 
      841 ggtgtgtggc tactgtctgc atcctcctcc cccctacagc ctccccgcct cccctccagc 
      901 caccctggga ttggtgactc tcagcccctc ccctcagctc ccctagaccc tcccagagcc 
      961 tttatcaggg agctgggact gagtgactgc agccttccta gatcccctcc actcggtttc 
     1021 tctctttgca ggagcaccgg cagcaccagt gtgtgagggg agcaggcagc ggtcctagcc 
     1081 agttccttga tcctgccaga ccacccagcc cccggcacag agctgctccg gcgcgccgaa 
     1141 gttcctattc ttcaaaaggt ataggaactt ccgccaccat ggtgagcaag ggcgaggagc 
     1201 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt 
     1261 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca 
     1321 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg 
     1381 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg 
     1441 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca 
     1501 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg 
     1561 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca 
     1621 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga 
     1681 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc 
     1741 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc 
     1801 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg 
     1861 ccgggatcac tctcggcatg gacgagctgt acaagttatt tctgaactgc tgcccgggct 
     1921 gctgcatgga accggatcca aaaaagaaga gaaaggtaga tccaaaaaag aagagaaagg 
     1981 tagatccaaa aaagaagaga aaggtaatat aagaagttcc tattctctag aaagtatagg 
     2041 aacttcagcg gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac 
     2101 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg 
     2161 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 
     2221 atttcacaaa taaagcattt ttttcactgc gaattctcgt aaaactcttc atggcagtag 
     2281 ttattattct ctctctctct ctttttcttt tttcttgaga caggatattt ctctgttgcc 
     2341 caggctggag tgcagtggca cagtcttggc tcactgcagc ctggacctcc tgggctcaag 
     2401 ccatcctccc acctcagcct cccaagtagc tggggctaca ggcacatggc caccaggccc 
     2461 agataatttt tcatttttgt agagactgag tctcaccatg ttacccaggt ttattattct 
     2521 cattttttag atgaagagac tgaggtccag agaagctcaa tgacttgcct agttttacaa 
     2581 atctcctgcc atcacatacc cctcagcgtc cttaataaga gggaggccac caactatgtg 
     2641 ctgggcactg tggtggatgc tggagctata gggttgagta tataagaaat ggtgttgctg 
     2701 gagcaactgt tgcttgctta cctgacctat ctgagaatta attagcaggg gaacatattt 
     2761 ttgttttcag attcaatata agaacttgtg tgggcaaaaa taaagatcag tagtaataac 
     2821 agtagttccc atttgctgac tgtactgtcc taagtgcata tatatataca tacacacacg 
     2881 catacctata ctcctctaat actcaaaatg atcctgttta tgtattgtta atatgctcat 
     2941 tttattttta aatttttatt tatttttatt tttatttatt tttgagacgg agtctcattc 
     3001 tgtcgcggag gctgaagtgc agtggtgcga tctcagctca gtgcgacctc cgcctcccgg 
     3061 gttcaagtga ttctcctgcc tcagcctccg gactagctgg gattacaggc gcccgcctcc 
     3121 acgcccagct aatttttgta tttttagtag agatggggtt tcgccatgtt ggccaggctg 
     3181 gtctcgtact cctgaccttg agtgatccac ctgcctcggc ctcccaaagt gctgggatta 
     3241 caggcatgag ccaccgcgcc gggctaatat gctcatttta gtgaggcaaa aatagaggct 
     3301 cagagtctga tttgtacaaa actacagagc agttaagtgt cctctcagat gtgtaccctg 
     3361 atctgggtga ctctaggact ctaggtctca actgttacaa ccagttaagg gtttggggaa 
     3421 gcactgggcc aagagtcagg aaaatggaag ccacaggtag tgcaaggtct tgggaatggg 
     3481 acgtctggtc caaggattca cgcgatgact ggaacccgaa gagccggggc ccggtttacg 
     3541 gccgccatga agcaacgcgc gccggtaggt ttgggaatca gggagccctc tgaataggag 
     3601 actgagttgg gagggaaagg ggcttcgctg ggggagcctc ggcttcttct gggagaaaat 
     3661 tcccacggct acctagtgag cctgcaaact ggtaggcgcc ggcgtaggcg cgcgggcggg 
     3721 gccgggggcg gggcctgcgg ggcgtggcgg ggcgggcaga gggcggggcc tgcttctcct 
     3781 cagcttcagg cggctgcgac gagccctcag gcgaacctct cggctttccc gcgcggcgcc 
     3841 gcctcttgct gcgcctccgc ctcctcctct gctccgccac cggcttcctc ctcctgagca 
     3901 gtcagcccgc gcgccggccg gctccgttat ggcgacccgc agccctggcg tcgtggtgag 
     3961 cagctcggcc tgccggccct ggccggttca ggcccacgcg gcaggtggcg gccgggccct 
     4021 gaggcgcggg atcgatccat gacacccccc cgccccccat atctcactat aattcactgt 
     4081 caaatggctt ttatgatttg gagtttgggg atacttttaa agacagggtc ttggtagctc 
     4141 ctagatggct tgggattcac ttagtaaccc ttgctggcct aaaattcagg aacttctacc 
     4201 tcatcttccc aagtactggg ttagtaggga tgtccagcat atttagcttg ctttaatgat 
     4261 tttgctgtac ttttttctgt gtgtacaggt gcatgtatat ttataaaact aacataatat 
     4321 attttacccc caaagtacca taaccctaat taatgctatt aaatgttttg tatgctgggc 
     4381 tctgggcata ctaatataac caatattata caatgcaaaa caattttgga atatcttctg 
     4441 taaaattatc ttattagtaa gttgtaacca tctcagactt ctaagtttga gtttgtaaag 
     4501 gatcagtttt ttttggtgac ctacacacac acacacacac acacacacac acacacacac 
     4561 aggggggtgg attctgagaa tttagagaat ttagatggtt aataccagtt tttattttta 
     4621 ttctgtgtga tatttattta ttaagattta tttatttatt acatgtaggt acactgtatc 
     4681 tgtcttcaga cacaccagaa gagggcatca gatctcgtta cagatggttg tgagtcacca 
     4741 tgtggttgct gggatttgaa ctcaggacct ctggaagaac agtcagtgct cttaaccgtt 
     4801 gagccatctc accagccccg tgatatttta tttaacttac tagaatatac tgtgttaaaa 
     4861 catattttag gggattgggg agatggccca ttctgtaaag tgtctgccat gtcatcttca 
     4921 ggacctgcgt ttgtaacccc agcatggaga agacagagac agggagaccc ctgggcttcc 
     4981 tggcaagcta gactagcaaa tttggttaac taggttcaat gagagactca gtctcaaaac 
     5041 ataagaagtg aattttttag acccaatttc aatctctgaa ctccatacgt atgcacatgt 
     5101 acctgcatat aaatatatat acacacccat aaacacatgc acacacaaaa aatggttcaa 
     5161 aagttcttaa aatgtataag agtagaaagt gtagagccgg gagtggtggc gcacgccttt 
     5221 aatcccagca cttgggagca gaggcaggtg gatttctgag ttcgaggcca gcctggtcta 
     5281 cagagtgagt tccaggacag ccagagctat acagagaaat cctgcctcaa aaaaccaaaa 
     5341 ataaagtgta tatactaatc tttcatgcct aaaatattta tttttgccac atttttcata 
     5401 cctctttaat atttttcttt ttctctttgt gcttcaaagg aaattttgtg tcatgtttta 
     5461 cttttcattc ttgactctat tcagcagtaa gacgcagcat ggctttctta gagaagtata 
     5521 atctattaca aaacggtaac atttaactgc tctacatacc ctcttgtagc taaattcctc 
     5581 tgttaaacta agacttatta tctaaacgta attaaatgtt gatatcttat aatgctccac 
     5641 tttgaaacag ctgtctttct tttgcagatt agcgatgatg aaccaggtta tgacctagat 
     5701 ttgttttgta tacctaatca ttatgccgag gatttggaaa aagtgtttat tcctcatgga 
     5761 ctgattatgg acaggttagt aagacctcga ttgaagttat ttttagaggg tgtgtgtgtg 
     5821 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt aagagagaga ggagaggcat 
     5881 gtgtgatata tatgtgaagt gtattgcatg tacatggaat ccagagaagg acattaggtg 
     5941 tcctactctg ttgctctctt attcccttga atcagtgtct cttgctgaac ctggggcata 
     6001 catactgatt tggctaggct gcctggctag caagacccag ggatctgctt gtctctgccc 
     6061 cccagtgctg acgttacaag caggtgcaac cacatctggt ttttatatgg gtactggggg 
     6121 atctgaactt agggcctgat gcgtgcccaa gtgctctcac ccgccaagcc acctctccag 
     6181 ccaaagggtt ttttttcttg tattaattct aacttagcaa ctgttgagta gttgatgttt 
     6241 gaatataagc taagccaact tattagaata aatgttttct attttttact tgtatgtgtg 
     6301 agtgcctgca tgtatgtatg tgaagcatat gtattccagg atgtcagaat tgtgatggat 
     6361 tccctggaac tggagttaca gaccattgtg aaccactgtg tatgggtgct gggaactgaa 
     6421 ctttaggtcc tctgcaaaaa cagtaaggat cttaactgat aaactatctt tctagcccta 
     6481 aggctttcta cttttaaagt tcgtttctct ttctctctct ctctctctct ctctctctct 
     6541 ctctccctct ccctccctcc tcctcctcct cctctttccc ccctctcgcc tcctctttct 
     6601 ctctcccctc cctctctctc tctcacacac acacacagac agagagagga gagagaaagg 
     6661 aagccttact aattaattac ctagttttag acataactag tgggaatcca tagtaagtaa 
     6721 atcaacattc ttgaagagag gttagaagaa ttggctctcg ttgtccatcg aggctgtata 
     6781 agattattct tctgttttct cagcttacca tacatatttt ctacgtagac aataccaatt 
     6841 caaatacact gtacgtaaag tatatgcgca cacacacaca cacacacaca cacacacaca 
     6901 cacaagttac atattgtcat atagtactaa aatttatgga ctttttcata ttaatacatc 
     6961 tagagctatc atgtttttta tcttttaaaa attactttac ggggctacag agatgtctca 
     7021 gcagttagga gcactgactg ctcttccaga ggtcctgagt tcaattccca gcaaccacat 
     7081 ggtggctcac aaccatctgt aatgggatct gatccactct tatggtgtgt ctgaagacag 
     7141 tgacagtgta ctcatataca tgaaataaat ctttaaaaaa ttactttacc atcagggtat 
     7201 atatatatat atatatgtgt gtgtgtgtgt gtgtgtgtat gaagtatttg ctatttgaga 
     7261 gggtctcact gtttagctca gtctggcttg gaacgttctg tatagcccag actggcctca 
     7321 actcagcagt ccccctgaaa ggctctcata tgtggagatt acaggtatgt gctactgtgc 
     7381 ccagccctac atgttacttt gtttttgaaa tagggtatca aatagcttag actggcttct 
     7441 aagtcagtgt gtagctaagg atggtctgag cttctgaacc tgctacttca actcctggtg 
     7501 tgccatcatg gccagtttgc acagttgtga gagtagaact caggacctcc tatgtgttac 
     7561 tagacatgtt ctctaccagc tgagctacat cccaattcgc ctacatttac ttttattttt 
     7621 taaaagattt atttatgtat acagcactct gcctgcatgt acatctgcat gccagaagag 
     7681 ggcaccagat ctcatcatac atagttgtga gccaccatgt ggttgctggg gattgaactc 
     7741 agggcctctg gaagagcagt cagtgctctt aacctctgag ccatctctgt agccccctac 
     7801 atttatttta aagattatag gctattctat tcatagatgt gccataactg atttaaccag 
     7861 atcttcatta ttttgtactt agtagagtat attagaggga atgaatctta aatacacatt 
     7921 ttgacacttg ctcagcttta gacaagtaaa aatcactggt caaggaaatg gtgcttagtg 
     7981 gatgcttctg aactgtcaaa ttgtcctcca acaagttaca tcattttata cttccaacgt 
     8041 tatgtgagag ttcatttttc tttaggctct tctagtattt tatatttgag aaataaaatt 
     8101 taaccaaaat ttgccaatct agtaagcttg tttttatttg tatctttaaa ttttatgata 
     8161 gatgttatag tgtactctcc tctcccctcc cctcccctct cctcctcagc aggatcttgt 
     8221 tatgtagccc taagggatct aatacttact ctgtagtcca ggctgtcctt cagctcatga 
     8281 gacacaccat catgcttggc atgtttcttg ttctgaaagg tatctggttt taattttggt 
     8341 tgagctggtg tgttggtgta tatctgtaat ctcagcacag gcaagtagag acaaggatta 
     8401 tgattacagt ccagcctggg ctatttagag ggattctctt taatctttcc tcatgcccca 
     8461 aaatcttacc tttggtatat gaaaaatagt ctccacttct gcaaaatatt gctttatgaa 
     8521 gtaagaatta attcccttca tagagacaag gaatgtgtcc tgtaaaagtt taatgtgtaa 
     8581 gaagtatttg ttataaaaga taaatattca gaatcttctt tttaattcct gattttattt 
     8641 ctataggact gaaagacttg ctcgagatgt catgaaggag atgggaggcc atcacattgt 
     8701 ggccctctgt gtgctcaagg ggggctataa gttctttgct gacctgctgg attacattaa 
     8761 agcactgaat agaaatagtg atagatccat tcctatgact gtagatttta tcagactgaa 
     8821 gagctactgt gtaagtataa ttaacttata attaaaaaaa tagggccatt ctagttttat 
     8881 ctatattttt tttaaacttg tgcaaactat gctacataat caacttactt gattatgtaa 
     8941 tatatatagt aatagttgga attactatta ttacatattt atttttctgt gctggggtag 
     9001 aacccaggga actaagtact gtaataaccg agcttcattc atcctagtca tttttaattt 
     9061 tttttttttt ttttgttttt tcgagacaag gtttctctgt gtagccctgg ctgtcctgga 
     9121 actcactctg tagaccaggc tggcctcgaa ctcagaaatc tgcctgcctc tgcctcccag 
     9181 tgctgagatt aatgacgtgc accaccactg cctggcattt ttttaaagtt ttaatttgag 
     9241 accctttctc actaacttgc tcacactatc ctcgaactca ttacgtgggc tggtctagaa 
     9301 atttcagtca acttgcctca gcttctcaag tagctgggat tacaggcagg tgctaccact 
     9361 tctggccatg atcattgttg ttgttgctat tattattatt attatttcac tgatacttca 
     9421 tatcacaaca cagaatagta actgtagttc atgaccaaca aaaaagttgt acagtttctc 
     9481 tgttgtctta ttttgatctg gattttgatt tgtttcatgg cactgggcag gctaatctca 
     9541 aactgcatgg ctcaaatagt ccttctgtct taatctccca tgttctggaa gtataggtat 
     9601 cactacacct agctgtattt tcatatttta aaaaaaacgc ctaagaattg ctattgaatg 
     9661 aggcggagag atggctcagc agtcacaagt acttgtagag gactggggtt tggttactag 
     9721 cacctcaaag atggcttaca attatctgta gctccaattc cagaggatct gtgtggtaaa 
     9781 gctgcatgta tatgggcatg caggtaaaac tgctaaacat ataaaaatag ctcagtctta 
     9841 agagaattgg tcgacttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 
     9901 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag 
     9961 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 
    10021 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg 
    10081 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca 
    10141 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt 
    10201 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga 
    10261 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga 
    10321 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat 
    10381 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca 
    10441 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt 
    10501 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac 
    10561 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct 
    10621 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga 
    10681 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac 
    10741 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat 
    10801 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg 
    10861 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc 
    10921 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc 
    10981 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg 
    11041 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta 
    11101 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 
    11161 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 
    11221 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccaggtcg 
    11281 agtagggata acagggtaat ggatcctcgt tgggcacctg cactcatgta cgcaggccca 
    11341 catatagatg tacacacgca tatcattcaa aatagaaatt atttatacaa gtagtgcgtt 
    11401 ggagagctgg ctcaggagtt gttaatgctt gctacccttg caaaggaccc aagttcaatt 
    11461 tccagcacct gtgttgggca tctcacaacc acatgtaaat ctagtttcag gggatttcac 
    11521 ccctcttctg aactcttcag gcacttatac ccacaaatgg acatctccac atagagacac 
    11581 atacttttaa aaaagtaatc tagggtctag agaaatggtt atagaccacg gactgggatc 
    11641 agttcccaga agccacatcc tgtggctcac aattgcctgt gaatcaagtt ctagatctgg 
    11701 tggcctcgtc tagcctgcag tcatcaacaa acacatgcac acatccatac acacataatt 
    11761 taaaagaaag cgaaagtaac ctataatgct aaacaaagca cattctcagt gtagactgca 
    11821 gtggtgatga accttgaaaa tatattaata gaagccagac acgtaagtca caaatggtat 
    11881 aactcaattt atatgaaatt cccagagagt ccaaagttaa aaaaaacaaa aaggcagatt 
    11941 attggttgtc agtagctaca gagatgggac attgaagata ttaataggta tagggttgag 
    12001 gattcatggc ctttccctct tccatgttag catgtctatt gttgttaacc ttattcaact 
    12061 catgttaggg caatcatatt gatgagactt aatgagtata gcttgacatt cctagaagac 
    12121 agagtctcac atcagactct ctgatcctct gactcttaga atctttcaac accctcttct 
    12181 gaaatgatct ctgagccttt ggttgtgact gaattatgag aaagaatttt atgttgaatg 
    12241 aattttactt cagcattttt acaaaactga agctggggat ggcgtttcat gcgtgcagtc 
    12301 cctaccattt gcctataagc ctggaggagg cagaagtaga attaggcttt tcagggacat 
    12361 cttcagctgc atcatgagct tgaggacaac ttgagctaca taagattccc atccccaccc 
    12421 ccacacacac acacacatct agtatgtacc tagtatgcag tcttttcata tgtttaaaat 
    12481 gtagaaaggt atatgtgaag tctccctttt ccccacaact tagtccctcc aaagtattgc 
    12541 atcagtttcc ttgtacatga ttttatgcat atataagata cactatttct ctctctctct 
    12601 ctctccttcc ctccttccct ccaacctctg ggtatgagtt gagggttatg tatgtttcaa 
    12661 gttgtaataa gaattgccac tcaaacattt catcccattt taagttaagt ttgtctgatt 
    12721 ttccacttta gaagggcagc catgatggct catgcctata atcccatcct agcacttgta 
    12781 agcctaatac ataagactgg aggattgtca tcagcttgag gctagcctgg tctacctaat 
    12841 gagttccagc cagccaagag tagaatataa gaccctgtct ccaaaaaatg taccaaaggg 
    12901 gctggagggg taactcagtg gttaagaaca gtagctgctc ttccagagga cctgggttca 
    12961 attcccagca cccacatggc agcttgcaac tatctgtaac tgtccagctt tgggatatcc 
    13021 gacaccatta taccaattac ataaaatgaa aaaattgtaa aatgtgctaa aatgagtggg 
    13081 gagggggagc atggtgtggt tctgtgggct tgggattttg ccatgaatcc atgactatat 
    13141 ctcagcaata tgtggattat ttctggaaac ctgtggggca tgggtgtatg ggaagagggg 
    13201 agagagagag agcccgcatc cagccagagt tcctgtgctc tgggcaggcg gacgtgggag 
    13261 gactgctgga cgatttccac ttggccctgg gtgggcatct ggctgtgtga agccactgac 
    13321 cccactcagc ggggggtaga taaggggcat ttcccatacc aggttctcag actccagcat 
    13381 tccacgctgg atacagcaga agagctgaga acagaatagg ggccccaggg agacactctc 
    13441 gtcccagaga taaagaaggc agagggagag agattcctac gcaggcgaga ggccttagtc 
    13501 tgggccttga tgaacagaga cggtctatgg ttttagagct ttattgtaga aaggcagggg 
    13561 gaaagagaga aggtagaaag agagagacca gccatggcca agaggagaga aggggggaaa 
    13621 ggagagagag aagaaaggct agagagtaag aaaggttaaa gagacagaag taaaagctta 
    13681 gagtaagaga gagagagaga gcaagaggga gagtaagaaa agtaaaagct taagagagcg 
    13741 aggaggggcc agcccctcta atagtgggct tgttatcttg ttgttgctag gtaactgtga 
    13801 agcgtctagc ctgaaggcca gaagcttggg acattgtcta tgtgactgaa agccatgagc 
    13861 ctctactgtg ggggctgtgg aggtggtaac ttggacagga gccaggggtt ccaggagaca 
    13921 tgagggaatg ccttctgtcc catgtaggta ggaattacca ccaccggctt tcaccgtagt 
    13981 tcactggaga ctgtttgtac atagcccatt gccccacaga aaccaggcac tcactgtttc 
    14041 atttgcataa gaattagaag gctttatttt ggctgggatt ggtgacttgc cccagaaatt 
    14101 ccagcttttg ggaagccgaa tcctggagaa ccaggagttc aaggtcatcc tcaggtacct 
    14161 catttgttca agaccagcct gtgctaccat gagaccttgt ccctactccc ctcaacccca 
    14221 aaacacgctg ttttcatttt ataggttgta ctaaccatgg aggatggtca tttgcctttc 
    14281 tcttgatttc agaagcgtag cgcgggcttt gcaccatgat ctagtgtttc cagataggct 
    14341 tgcctctcca cagccaggct tatcctgctt cagtcccatg tttggcaagg gcaacatatg 
    14401 actgaagaac ttgcatagcc ttatggtgta tcatgactct tagccccagt ttataactgt 
    14461 ctaacagctg gtggcttttg tgaaaaagga aagcatcatt tgaactttta acttttcagc 
    14521 tgtttgtttt gactttgcat aaatactgag actagctagg aaatgagctt ttgtttcatt 
    14581 tgctaaaaat gatttttttt tcatgaggca atttcaagca atttgaattt agcaatctct 
    14641 acttgtctga aaagaagtta ttaattaaaa aaaaacaaac aaacaggaac ctattatgct 
    14701 ggctagtcac atgaaagaat ccaaatgaac acagtcaagg ctgaatcaac acatgaaatt 
    14761 attttctaat tccccaaatt acatttatgc taattagtat gtagtaaaac attttaattg 
    14821 tttccaatgt taaacattac agaaataatt ttgatgtttt cctaataacc cagcctttga 
    14881 tcatgtttgc atttatttga ttgttgtcag tttattaaat aattaagtaa gagcaacaag 
    14941 ataaaatgac ttcatgagag tccttttctg taacttagaa cgtcagtagt cataggaact 
    15001 gcggtcgttc ccttaccttt cagaagtagg ctaaagagtt gaacgcaaag gtggtttttc 
    15061 gaatcttcct gatccttaag cccccactag gtttatattc tatatttgat atcggattcg 
    15121 aacccctcga gcttaggatc cggaaccctt aatataacta agttattagg tccctcgaag 
    15181 aggttcacta gtactggcca ttgcggccgc ttcgaggctg cctcgcgcgt ttcggtgatg 
    15241 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg 
    15301 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg 
    15361 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc 
    15421 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 
    15481 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 
    15541 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 
    15601 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 
    15661 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 
    15721 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 
    15781 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 
    15841 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 
    15901 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 
    15961 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 
    16021 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 
    16081 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 
    16141 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 
    16201 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 
    16261 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 
    16321 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 
    16381 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 
    16441 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 
    16501 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 
    16561 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 
    16621 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 
    16681 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 
    16741 caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 
    16801 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 
    16861 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 
    16921 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 
    16981 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 
    17041 ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt 
    17101 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 
    17161 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 
    17221 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 
    17281 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 
    17341 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 
    17401 ggttccgcgc acatttcccc gaaaagtgcc acctgacgg 
//
