LOCUS       pAAV-EF1a-hChR2-        7010 bp    DNA     circular     23-AUG-2007
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|452192721|
COMMENT     VNTDBDATE|455453634|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV-EF1a-hChR2-EYFP-WPRE|
COMMENT     VNTAUTHORNAME|Feng Zhang|
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COMMENT                          "Foreign object. Author: Feng Zhang. Original author: Feng Zhang" 
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FEATURES             Location/Qualifiers
     misc_feature    1..130
                     /dnas_title="L-ITR"
                     /vntifkey="21"
                     /label=L-ITR
     misc_feature    4412..4552
                     /dnas_title="R-ITR"
                     /vntifkey="21"
                     /label=R-ITR
     rep_origin      4643..4949
                     /dnas_title="f1 Origin"
                     /vntifkey="33"
                     /label=f1 Origin
     misc_feature    5468..6325
                     /dnas_title="AmpR"
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      6476..7143
                     /dnas_title="pUC ori"
                     /vntifkey="33"
                     /label=pUC ori
     misc_feature    636..641
                     /note="BamHI"
     misc_feature    651..677
                     /note="HA tag core seq"
     misc_feature    2395..2558
                     /note="+63 C term"
     misc_feature    648..650
                     /note="TAL N-term"
     misc_feature    642..647
                     /note="kozak"
     misc_feature    139..144
                     /note="MluI"
     misc_feature    145..150
                     /note="BsrGI"
     misc_feature    4396..4402
                     /note="RsrII"
     misc_feature    4384..4390
                     /note="BstEII"
     misc_feature    4152..4383
                     /note="bGH polyA"
     misc_feature    40..45
                     /note="SmaI"
     misc_feature    678..1085
                     /note="136 truncation"
     misc_feature    151..711
                     /note="fp"
     misc_feature    740..745
                     /note="SalI"
     misc_feature    722..745
                     /note="rp"
     misc_feature    642..739
                     /note="oligo"
     misc_feature    151..666
                     /note="oligo fp"
     misc_feature    713..745
                     /note="oligp rp"
     exon            151..635
                     /dnas_title="Human Synapsin I promoter"
                     /vntifkey="61"
                     /label=Human Synapsin I promoter
     misc_feature    3530..4138
                     /dnas_title="WPRE"
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    4146..4151
                     /note="XhoI"
     misc_feature    3516..3521
                     /note="EcoRI"
     misc_feature    2565..2594
                     /note="GS linker"
     misc_feature    2868..2918
                     /note="NLS"
     misc_feature    2868..2873
                     /note="mutated"
     misc_feature    2907..2912
                     /note="mutated"
     misc_feature    2887..2890
                     /note="BsaI overhang"
     misc_feature    2595..3191
                     /note="cib1_hs_opt"
     misc_feature    3488..3512
                     /note="overlap"
     misc_feature    3192..3512
                     /note="cib1_hs_opt"
     misc_feature    3513..3515
                     /note="STop"
     misc_feature    2559..2564
                     /note="NheI"
     misc_feature    1086..2394
                     /note="TALE Repeats"
ORIGIN      
          1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
         61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
        121 aggggttcct gcggccgcac gcgtTGTACA gtgtctagac tgcagagggc cctgcgtatg
        181 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
        241 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
        301 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
        361 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
        421 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
        481 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
        541 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
        601 aggagtcgtg tcgtgcctga gagcgcagtc gagaaggatc cGCCACCATG TACCCATACG
        661 ATGTTCCAGA TTACGCTGTA GATTTGAGAA CTTTGGGATA TTCACAGCAG CAGCAGGAAA
        721 AGATCAAGCC CAAAGTGAGG TCGACAGTCG CGCAGCATCA CGAAGCGCTG GTGGGTCATG
        781 GGTTTACACA TGCCCACATC GTAGCCTTGT CGCAGCACCC TGCAGCCCTT GGCACGGTCG
        841 CCGTCAAGTA CCAGGACATG ATTGCGGCGT TGCCGGAAGC CACACATGAG GCGATCGTCG
        901 GTGTGGGGAA ACAGTGGAGC GGAGCCCGAG CGCTTGAGGC CCTGTTGACG GTCGCGGGAG
        961 AGCTGAGAGG GCCTCCCCTT CAGCTGGACA CGGGCCAGTT GCTGAAGATC GCGAAGCGGG
       1021 GAGGAGTCAC GGCGGTCGAG GCGGTGCACG CGTGGCGCAA TGCGCTCACG GGAGCACCCC
       1081 TCAACCTGAC CCCAGAGCAG GTCGTGGCGA TCGCAAGCCA CGACGGAGGA AAGCAAGCCT
       1141 TGGAAACAGT ACAGAGGCTG TTGCCTGTGC TGTGCCAAGC GCACGGACTT ACCCCAGAGC
       1201 AGGTCGTGGC AATCGCCTCC AACATTGGCG GGAAACAGGC ACTCGAGACT GTCCAGCGCC
       1261 TGCTTCCCGT GCTGTGCCAA GCGCACGGCC TTACCCCAGA GCAGGTCGTG GCAATCGCGA
       1321 GCAATAACGG CGGAAAACAG GCTTTGGAAA CGGTGCAGAG GCTCCTTCCA GTGCTGTGCC
       1381 AAGCGCACGG ACTAACCCCA GAGCAGGTCG TGGCAATCGC CTCCAACATT GGCGGGAAAC
       1441 AGGCACTCGA GACTGTCCAG CGCCTGCTTC CCGTGCTGTG CCAAGCGCAC GGGCTAACCC
       1501 CAGAGCAGGT CGTGGCAATC GCGAGCAATA ACGGCGGAAA ACAGGCTTTG GAAACGGTGC
       1561 AGAGGCTCCT TCCAGTGCTG TGCCAAGCGC ACGGCCTAAC CCCAGAGCAG GTCGTGGCGA
       1621 TCGCAAGCCA CGACGGAGGA AAGCAAGCCT TGGAAACAGT ACAGAGGCTG TTGCCTGTGC
       1681 TGTGCCAAGC GCACGGACTT ACACCCGAAC AAGTCGTGGC AATTGCGAGC AACGGAGGGG
       1741 GAAAGCAGGC ACTCGAAACC GTCCAGAGGT TGCTGCCTGT GCTGTGCCAA GCGCACGGAC
       1801 TTACCCCAGA GCAGGTCGTG GCAATCGCGA GCAATAACGG CGGAAAACAG GCTTTGGAAA
       1861 CGgTGCAgAG GCTCCTTCCA GTGCTGTGCC AAGCGCACGG CCTTACCCCA gagCAgGTCG
       1921 TGGCCATTGC CTCgAATGGA GGGGGCAAAC AGGCGTTGGA AACCGTACAA CGATTGCTGC
       1981 CGGTGCTGTG CCAAGCGCAC GGACTAACCC CAGAGCAGGT CGTGGCGATC GCAAGCCACG
       2041 ACGGAGGAAA GCAAGCCTTG GAAACAGTAC AGAGGCTGTT GCCTGTGCTG TGCCAAGCGC
       2101 ACGGGCTAAC CCCAGAGCAG GTCGTGGCGA TCGCAAGCCA CGACGGAGGA AAGCAAGCCT
       2161 TGGAAACAGT ACAGAGGCTG TTGCCTGTGC TGTGCCAAGC GCACGGCCTA ACCCCAGAGC
       2221 AGGTCGTGGC CATTGCCTCG AATGGAGGGG GCAAACAGGC GTTGGAAACC GTACAACGAT
       2281 TGCTGCCGGT GCTGTGCCAA GCGCACGGAC TCACGCCTGA GCAGGTAGTG GCTATTGCAT
       2341 CCCATGACGG GGGCAGACCC GCACTGGAGT CAATCGTGGC CCAGCTTTCG AGGCCGGACC
       2401 CCGCGCTGGC CGCACTCACT AATGATCATC TTGTAGCGCT GGCCTGCCTC GGCGGACGAC
       2461 CCGCCTTGGA TGCGGTGAAG AAGGGGCTCC CGCACGCGCC TGCATTGATT AAGCGGACCA
       2521 ACAGAAGGAT TCCCGAGAGG ACATCACATC GAGTGGCAgc tagcggcggc ggcggcagcg
       2581 gcggcggcgg cagcAACGGC GCGATTGGTG GGGATTTGCT GCTTAACTTT CCCGACATGT
       2641 CCGTGTTGGA ACGTCAGCGC GCACATTTGA AGTATCTTAA CCCCACCTTC GACTCCCCGT
       2701 TGGCCGGGTT CTTTGCGGAC TCATCTATGA TTACGGGAGG GGAAATGGAC AGCTACCTCT
       2761 CAACGGCCGG ATTGAATCTT CCGATGATGT ATGGAGAAAC CACTGTAGAA GGCGACTCGC
       2821 GACTCTCGAT TTCGCCTGAA ACGACGCTGG GAACAGGGAA CTTCAAGGCG GCGAAATTCG
       2881 ACACGGAGAC AAAAGATTGC AACGAAGCGG CGAAGAAAAT GACCATGAAT CGCGATGATC
       2941 TGGTAGAGGA GGGAGAGGAG GAAAAGTCGA AGATTACTGA ACAGAACAAT GGGTCTACCA
       3001 AAAGTATCAA AAAGATGAAG CACAAAGCTA AGAAAGAAGA GAACAATTTC AGCAATGACA
       3061 GCAGTAAAGT CACAAAAGAA CTGGAGAAAA CGGATTACAT TCACGTGAGG GCGCGACGAG
       3121 GGCAGGCTAC AGATTCACAT TCAATTGCGG AGAGAGTACG GAGAGAGAAA ATCTCAGAAA
       3181 GGATGAAGTT CCTCCAAGAC CTTGTGCCAG GTTGTGACAA GATCACAGGC AAAGCAGGAA
       3241 TGCTGGATGA GATCATCAAC TACGTCCAAT CGTTGCAAAG ACAAATTGAG TTTCTCTCGA
       3301 TGAAACTGGC CATCGTGAAT CCTAGACCGG ATTTCGACAT GGATGACATC TTTGCGAAAG
       3361 AAGTGGCATC CACTCCCATG ACGGTTGTGC CCTCACCGGA GATGGTCTTG TCTGGTTACA
       3421 GCCACGAAAT GGTGCATTCG GGTTATTCAA GCGAGATGGT CAATTCGGGA TACCTTCACG
       3481 TCAATCCCAT GCAGCAGGTG AATACTTCCA GTtaagaatt cgatatcaag cttatcgata
       3541 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
       3601 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
       3661 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
       3721 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
       3781 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
       3841 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
       3901 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
       3961 cctAtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
       4021 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
       4081 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgag
       4141 cgctgctcga gCTAGAGCTC GCTGATCAGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC
       4201 TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT
       4261 TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG
       4321 GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG
       4381 GGAggtaacc acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact
       4441 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg
       4501 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg
       4561 atgcggtatt ttctccttac gcatctgtgc ggtattcaca ccgcatacgt caaagcaacc
       4621 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
       4681 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
       4741 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
       4801 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
       4861 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
       4921 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga
       4981 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
       5041 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac
       5101 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
       5161 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
       5221 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
       5281 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
       5341 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
       5401 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
       5461 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
       5521 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
       5581 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
       5641 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
       5701 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
       5761 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
       5821 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
       5881 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
       5941 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
       6001 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
       6061 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
       6121 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
       6181 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
       6241 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
       6301 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
       6361 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
       6421 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
       6481 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
       6541 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
       6601 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
       6661 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
       6721 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
       6781 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
       6841 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
       6901 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
       6961 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
       7021 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
       7081 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
       7141 tcacatgt
//

