LOCUS       Exported               12919 bp ds-DNA     circular SYN 10-JUL-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pNOC-CRISPR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 14, 2017 from SnapGene Viewer 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12919
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      418..1418
                     /label=CS terminator
     misc_feature    1425..1501
                     /label=HDV
     misc_RNA        complement(1502..1577)
                     /label=g RNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        1606..2789
                     /label=Ribi
     CDS             2800..2820
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2821..6921
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6958..7473
                     /codon_start=1
                     /label=Nlux
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILAG"
     CDS             7474..7500
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7516..7536
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7568..8167
                     /label=LDSP terminator
     promoter        8254..9023
                     /label=LDSP promoter
     CDS             9048..10073
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      10074..10304
                     /label=35S terminator
     rep_origin      complement(10482..11070)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11241..12101)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12102..12206)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(12284..12739)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     12880..12896
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 atcactagag tatagctaca tggtagctag tagtcgtcaa aggaaataaa gacatagcgt
      121 aaagtataca aaatgacttc cgcggcgcgg gagcggcagg cagcacttgc aaaacgaagc
      181 ccaatgggcc agattacggt caacctcgtc attttattac ggtcgtcagc aagagcggtt
      241 tagaccgtcg ccggcacggg tggctcccga tattcttggc tttcgtgggt ggaggcggcg
      301 tgtgactcct catctttaag tagtggtcgc ttttgctgca tgcttttgat cacctcgtga
      361 ttgatggctg gattctcagc tgtcttctga catctttggg tgcatgggtt gaccggtgca
      421 aagctgacgc ccttttcgtc gtcgttgctg gtgttaaaat tgcgcgtttc ttttttttcc
      481 gtctttgtcg tcttttgact tttttatctt cttttctgtc ttcttttatt cttctcttct
      541 tcttcatcac tccctcttca tttttcttct tctttttcct cgtctattgt cctcgggcat
      601 taccagttgt gctgctcacg tgcgcgcaag cggtgaaagg gtgagaggat acacggacaa
      661 caatcattga aaattacgct taaagccggt gcctggaaaa gtccacgcgc agatggcctt
      721 tgcttgcctt gctgctgctg cagctgctgc tgctgtcgcc tgtgatggta atggttgttg
      781 ttgtggtcct gcttgcttgc tagtttgcat tgcgtttgcg gttgccttat gaccgactcc
      841 gtgtgattga tccttaaaca tattttatca ttgatgtgga ttgtgtgacg acggcaaaaa
      901 agaattactt tacacatgac tatcacacct gctcccctcc cttctccttt caatcggtca
      961 acttcccaac cgctcctcga aaatatttca gttgaatcct ctactacgta ccccttactt
     1021 aaatttactt tctctctccc catcaatgcg ccgttgttct gaagttgtat tattgatggg
     1081 agatgattgg cactgcacca gacctggcca aaagccacca caggtggcca catactcttg
     1141 catgggtata ctctaccgag gcaaagatta aagtgcacgt cccctgcccc tctctctccc
     1201 tacttccttc agtctcagtg gttccgtgcc cgttcagcac tacatcgccc tgcccgctgt
     1261 tccttctcaa acagccaccc atcccaaccc acatacacag aaacaacctt tcctacacat
     1321 tttcccaagt cttctcttca ctattctaca ctaatacacc accgtgatat gccggttatc
     1381 tagatgtcct ttctcctttc tcatcctttc tttcccatgg tacccaggag tcccattcgc
     1441 catgccgaag catgttgccc agccggcgcc agcgaggagg ctgggaccat gccggccaaa
     1501 agcaccgact cggtgccact ttttcaagtt gataacggac tagccttatt ttaacttgct
     1561 atttctagct ctaaaacgaa gagccaaggc ctgctcttca tcgattctgt ggatggaggg
     1621 agggaaggag ggggggagtg agtgagtcga gagaacgacg actggggcaa agagaggatg
     1681 acgccactgc aacaaaagac agggaagaat agaggctgtt ggaaaacagg aacacagtga
     1741 agaatagagg ctgcagggta acaggcacat ttatcgcaga gaggcgggcg gcaggggcga
     1801 tacgtaaaga gacccctaaa cttctccgcg tcatccgttc acgctacctc cttccctccc
     1861 tttactccca ttgatcacca aggcagcgcc cgcagccacc agcagccacc gtcctcagtg
     1921 tcacagctag acgcgtcccc atcaggcagc agaccaccag aatatgcgct ttcgcttccc
     1981 atcatatccc tccctcctcc ccttctattc ctcatcatgc atcatttctt tctccttgct
     2041 gcatgcccct cttcttacct tggctaagat ctaccggagg aagaagtctt caacgataga
     2101 taaatgcctg tatcgtgatg gttgtttgaa tggtggaagt gagccaggta ttgagaacga
     2161 ggggcgcaag ggtgcgtgga agaaggcagg gagaggcgag gcgaaggaga agggcatgtg
     2221 gtgggaacga agcctccaca ccacagccac ccatgctttt ctctgttcga gttcccgggt
     2281 tctactctca cacactacac gtgcacagaa aacattcacg cacaacagag atacagaggg
     2341 atccgtcgag atggtctaca cgtcctaatg tcccctgctt tcgaagccca gagccatcgc
     2401 tttctgtttt tatccattcg caacacatcc tgttctcgcg acctctcctc ttcctggcca
     2461 ccatcgcttc tccgtgccat gccctgtaca tccttccctc cctcgttgtc taccacacta
     2521 atcatgcgct ctgcagcaga cactcggcta ctatttgtgt cgtacggatt gaggggaggg
     2581 aggggaaggg aggcaagaag taaatcggtg gtcaacaaca gacgcggtct ccccaccctc
     2641 cctcattcct ttcccctcct ataatgagcg aagaaagcca tggacaccga gtcacccact
     2701 ctcctaatcc accaccacca gggcactaac ggactcacgc tcaccaaact cggtaacaat
     2761 atccactgca cacagataca cacgcaacac tcgagcatgc ccaagaaaaa gcggaaggtg
     2821 gacaagaagt actccattgg gctcgatatc ggcacaaaca gcgtcggctg ggccgtcatt
     2881 acggacgagt acaaggtgcc gagcaaaaaa ttcaaagttc tgggcaatac cgatcgccac
     2941 agcataaaga agaacctcat tggcgccctc ctgttcgact ccggggagac ggccgaagcc
     3001 acgcggctca aaagaacagc acggcgcaga tatacccgca gaaagaatcg gatctgctac
     3061 ctgcaggaga tctttagtaa tgagatggct aaggtggatg actctttctt ccataggctg
     3121 gaggagtcct ttttggtgga ggaggataaa aagcacgagc gccacccaat ctttggcaat
     3181 atcgtggacg aggtggcgta ccatgaaaag tacccaacca tatatcatct gaggaagaag
     3241 cttgtagaca gtactgataa ggctgacttg cggttgatct atctcgcgct ggcgcatatg
     3301 atcaaatttc ggggacactt cctcatcgag ggggacctga acccagacaa cagcgatgtc
     3361 gacaaactct ttatccaact ggttcagact tacaatcagc ttttcgaaga gaacccgatc
     3421 aacgcatccg gagttgacgc caaagcaatc ctgagcgcta ggctgtccaa atcccggcgg
     3481 ctcgaaaacc tcatcgcaca gctccctggg gagaagaaga acggcctgtt tggtaatctt
     3541 atcgccctgt cactcgggct gacccccaac tttaaatcta acttcgacct ggccgaagat
     3601 gccaagcttc aactgagcaa agacacctac gatgatgatc tcgacaatct gctggcccag
     3661 atcggcgacc agtacgcaga cctttttttg gcggcaaaga acctgtcaga cgccattctg
     3721 ctgagtgata ttctgcgagt gaacacggag atcaccaaag ctccgctgag cgctagtatg
     3781 atcaagcgct atgatgagca ccaccaagac ttgactttgc tgaaggccct tgtcagacag
     3841 caactgcctg agaagtacaa ggaaattttc ttcgatcagt ctaaaaatgg ctacgccgga
     3901 tacattgacg gcggagcaag ccaggaggaa ttttacaaat ttattaagcc catcttggaa
     3961 aaaatggacg gcaccgagga gctgctggta aagcttaaca gagaagatct gttgcgcaaa
     4021 cagcgcactt tcgacaatgg aagcatcccc caccagattc acctgggcga actgcacgct
     4081 atcctcaggc ggcaagagga tttctacccc tttttgaaag ataacaggga aaagattgag
     4141 aaaatcctca catttcggat accctactat gtaggccccc tcgcccgggg aaattccaga
     4201 ttcgcgtgga tgactcgcaa atcagaagag accatcactc cctggaactt cgaggaagtc
     4261 gtggataagg gggcctctgc ccagtccttc atcgaaagga tgactaactt tgataaaaat
     4321 ctgcctaacg aaaaggtgct tcctaaacac tctctgctgt acgagtactt cacagtttat
     4381 aacgagctca ccaaggtcaa atacgtcaca gaagggatga gaaagccagc attcctgtct
     4441 ggagagcaga agaaagctat cgtggacctc ctcttcaaga cgaaccggaa agttaccgtg
     4501 aaacagctca aagaagacta tttcaaaaag attgaatgtt tcgactctgt tgaaatcagc
     4561 ggagtggagg atcgcttcaa cgcatccctg ggaacgtatc acgatctcct gaaaatcatt
     4621 aaagacaagg acttcctgga caatgaggag aacgaggaca ttcttgagga cattgtcctc
     4681 acccttacgt tgtttgaaga tagggagatg attgaagaac gcttgaaaac ttacgctcat
     4741 ctcttcgacg acaaagtcat gaaacagctc aagaggcgcc gatatacagg atgggggcgg
     4801 ctgtcaagaa aactgatcaa tgggatccga gacaagcaga gtggaaagac aatcctggat
     4861 tttcttaagt ccgatggatt tgccaaccgg aacttcatgc agttgatcca tgatgactct
     4921 ctcaccttta aggaggacat ccagaaagca caagtttctg gccaggggga cagtcttcac
     4981 gagcacatcg ctaatcttgc aggtagccca gctatcaaaa agggaatact gcagaccgtt
     5041 aaggtcgtgg atgaactcgt caaagtaatg ggaaggcata agcccgagaa tatcgttatc
     5101 gagatggccc gagagaacca aactacccag aagggacaga agaacagtag ggaaaggatg
     5161 aagaggattg aagagggtat aaaagaactg gggtcccaaa tccttaagga acacccagtt
     5221 gaaaacaccc agcttcagaa tgagaagctc tacctgtact acctgcagaa cggcagggac
     5281 atgtacgtgg atcaggaact ggacatcaat cggctctccg actacgacgt ggatcatatc
     5341 gtgccccagt cttttctcaa agatgattct attgataata aagtgttgac aagatccgat
     5401 aaaaatagag ggaagagtga taacgtcccc tcagaagaag ttgtcaagaa aatgaaaaat
     5461 tattggcggc agctgctgaa cgccaaactg atcacacaac ggaagttcga taatctgact
     5521 aaggctgaac gaggtggcct gtctgagttg gataaagccg gcttcatcaa aaggcagctt
     5581 gttgagacac gccagatcac caagcacgtg gcccaaattc tcgattcacg catgaacacc
     5641 aagtacgatg aaaatgacaa actgattcga gaggtgaaag ttattactct gaagtctaag
     5701 ctggtctcag atttcagaaa ggactttcag ttttataagg tgagagagat caacaattac
     5761 caccatgcgc atgatgccta cctgaatgca gtggtaggca ctgcacttat caaaaaatat
     5821 cccaagcttg aatctgaatt tgtttacgga gactataaag tgtacgatgt taggaaaatg
     5881 atcgcaaagt ctgagcagga aataggcaag gccaccgcta agtacttctt ttacagcaat
     5941 attatgaatt ttttcaagac cgagattaca ctggccaatg gagagattcg gaagcgacca
     6001 cttatcgaaa caaacggaga aacaggagaa atcgtgtggg acaagggtag ggatttcgcg
     6061 acagtccgga aggtcctgtc catgccgcag gtgaacatcg ttaaaaagac cgaagtacag
     6121 accggaggct tctccaagga aagtatcctc ccgaaaagga acagcgacaa gctgatcgca
     6181 cgcaaaaaag attgggaccc caagaaatac ggcggattcg attctcctac agtcgcttac
     6241 agtgtactgg ttgtggccaa agtggagaaa gggaagtcta aaaaactcaa aagcgtcaag
     6301 gaactgctgg gcatcacaat catggagcga tcaagcttcg aaaaaaaccc catcgacttt
     6361 ctcgaggcga aaggatataa agaggtcaaa aaagacctca tcattaagct tcccaagtac
     6421 tctctctttg agcttgaaaa cggccggaaa cgaatgctcg ctagtgcggg cgagctgcag
     6481 aaaggtaacg agctggcact gccctctaaa tacgttaatt tcttgtatct ggccagccac
     6541 tatgaaaagc tcaaagggtc tcccgaagat aatgagcaga agcagctgtt cgtggaacaa
     6601 cacaaacact accttgatga gatcatcgag caaataagcg aattctccaa aagagtgatc
     6661 ctcgccgacg ctaacctcga taaggtgctt tctgcttaca ataagcacag ggataagccc
     6721 atcagggagc aggcagaaaa cattatccac ttgtttactc tgaccaactt gggcgcgcct
     6781 gcagccttca agtacttcga caccaccata gacagaaagc ggtacacctc tacaaaggag
     6841 gtcctggacg ccacactgat tcatcagtca attacggggc tctatgaaac aagaatcgac
     6901 ctctctcagc tcggtggaga cagcagggct gacgggtccg gcagcgggtc cgttaacatg
     6961 gtgtttactc tcgaggactt cgtgggcgac tggcggcaga cagctggcta taacctcgac
     7021 caggtgctgg agcagggcgg tgtgtcctcg cttttccaga acctgggcgt ctccgtgacc
     7081 ccaatccagc gcatcgtcct gagcggcgag aacggcctga agatcgacat ccacgtcatc
     7141 attccctacg agggcctgag cggggaccaa atgggccaga tcgagaagat cttcaaggtt
     7201 gtgtaccccg tggacgatca ccacttcaag gtgatactgc actacgggac gctggtcatc
     7261 gacggtgtga cgccgaacat gattgactac ttcggccgtc cttacgaggg cattgccgtg
     7321 ttcgacggca agaagatcac ggtgaccggc accctgtgga acggcaataa gatcattgac
     7381 gagcggctga tcaacccgga tggctcgctg ttgttccgcg tgaccatcaa cggggtaacc
     7441 ggctggcgcc tgtgcgagcg catcctggcc ggatacccct acgacgtgcc cgactacgcg
     7501 tccatggtgg ctagcccgaa aaagaagagg aaggtctgac aattggaaag atccaagaga
     7561 gacgagtaga gatttttttt ttgggattga tgtttgtcgt tctttgagtt gtcgtcgagt
     7621 tacgcctttt gtaagaatgt tccgcaggag aggaggagga tgggcatgag tgagggtgag
     7681 agggcttgcc cgcttttttt tttaaaaacg ctgaagacgt ggttgtcaaa caaacccccc
     7741 atagaaacga ttttgttacg gtgcggtcca gacgtcactt gaatggctcc gcggaaaggc
     7801 cagggaggga aggggggagg gaggaaacat gaaacatgtt gaacggctca acagggtttg
     7861 ggggacaaga gaggtagcgc cctgatggac tgctccctcc cctcctttcc ctcaatgtct
     7921 cattcatcca tgcttccccc ttctctctct cccctccgtt ccatcccccg cgggcgtggt
     7981 agtggcgtga tgggatccac taaaatgtac gtgtaagaaa agccggtgag cttacgcttt
     8041 tgtgaaagtg ggagtacgag tgttgtgtgt gtgtgtagtg gtttcagacc ccagacagag
     8101 gcgaagcaga aaaagcagac gatgaagacg acgaagaaat gagcagtcta tttttatcgt
     8161 ggaaacagaa gaggtgatat cgtctcctgc aggcatgcaa gctgatccac tagaggccat
     8221 ggcggccgca ctagtggatc ccccgggatg catacttgga agatggagtg gatggaggag
     8281 gaggcgagcg tagcagcaag cgtgagttat acagccaggc acatgtcgca atccttcggt
     8341 ctcgggctta aaatccacgc actaatcacg ctgggccatg caaagagcaa tgccgaggcc
     8401 caccacacaa aacgctgtgt cgcgcgttgc ggcctgaagc ttcatacttc ttagtcgccg
     8461 ccaaaagggc tcgagagacg agacccgttg gcatgaccga tgttgttcga cgcggtttgc
     8521 ttcgtcacag tcgacgtgat tcaggaatct ggagcctgca gatcattttt ttcagcctga
     8581 tatcgttctt ttccactgag aaccatcaga ccaccttttc ttccattgtg tgaaggagta
     8641 ggagttgccg tgctgctttg tgggagacat ctgcgatggt gaccagcctc ccgtcgtctg
     8701 gtcgacgtga cgagcctctt cactgttctt cgacggagag acgcaagcga gacggctcta
     8761 gaccttttgg acacgcattc tgtgtgtgaa ctagtggaca gtgataccac gtctgaaagc
     8821 tcaccactgc ccatggtgca gctacttgtc acaaagtttt gactccgtcg gtatcaccat
     8881 tcgcgctcgt gtgcctggtt gttccgccac gccggcctgc cccggggcgg ggcaatattc
     8941 taaaatctca cgcaaaacac cgcacttacc cctcacacat attcgtgata gaccaccacc
     9001 aatctcagcc cgcatcaaca caggagggcc ccggggggca ataagatatg aaaaagcctg
     9061 aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc
     9121 tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg
     9181 gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc
     9241 ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg
     9301 agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg
     9361 aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg
     9421 ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca
     9481 ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg
     9541 tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg
     9601 ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc
     9661 tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt
     9721 cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc
     9781 agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt
     9841 atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg
     9901 atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg
     9961 ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac
    10021 tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa tagagtagat
    10081 gccgaccgga gtccgcaaaa atcaccagtc tctctctaca aatctatctc tctctatttt
    10141 tctccagaat aatgtgtgag tagttcccag ataagggaat tagggttctt atagggtttc
    10201 gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct
    10261 atcaataaaa tttctaattc ctaaaaccaa aatccagtga cctgagcttc ctcgctcact
    10321 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
    10381 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
    10441 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
    10501 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
    10561 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
    10621 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
    10681 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
    10741 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
    10801 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
    10861 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
    10921 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
    10981 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    11041 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    11101 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
    11161 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    11221 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    11281 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    11341 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    11401 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    11461 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    11521 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    11581 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    11641 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    11701 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    11761 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    11821 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    11881 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    11941 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    12001 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    12061 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    12121 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    12181 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgatgcggtg
    12241 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt aagcgttaat
    12301 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
    12361 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
    12421 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
    12481 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
    12541 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
    12601 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
    12661 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
    12721 gcgccgctac agggcgcgtc cattcgccat tcaggctgcg caactgttgg gaagggcgat
    12781 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
    12841 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat
    12901 tgtaatacga ctcactata
//
