LOCUS pRSET_a_-_MatryoshCaMP6 4932 bp ds-DNA circular 12-JUL-2017 DEFINITION . KEYWORDS "EGFPcp-LSSmO" FEATURES Location/Qualifiers misc_feature 169..192 /label="Xpress_EK" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 1279..1713 /label="EGFP (0-431)" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature complement(2333..2351) /label="T7 term RV" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 CDS 3275..3934 /label="AmpR" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 CDS 2421..2489 /label="LacZ alpha" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc rep_origin 4032..4714 /label="ColE1 origin" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 100..2163 /label="ORF frame 1" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 misc_feature 214..276 /label="M13 peptide" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 misc_feature 277..282 /label="LE linker" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 1720..2163 /label="CaM" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 misc_feature 1714..1719 /label="LP linker" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd primer_bind 20..39 /label="T7" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 rep_origin 2508..2814 /label="F1 ori" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 553..561 /label="GGT flexible linker" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 100..213 /label="pRSET tag" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 283..552 /label="EGFP (444-713)" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 562..1269 /label="LSSmOrange" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 misc_feature 1270..1278 /label="GGS flexible linker" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 112..129 /label="6x HIS tag" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 133..165 /label="T7 leader/tag" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc ORIGIN 1 GATCTCGATC CCGCGAAATT AATACGACTC ACTATAGGGA GACCACAACG GTTTCCCTCT 61 AGAAATAATT TTGTTTAACT TTAAGAAGGA GATATACATA TGCGGGGTTC TCATCATCAT 121 CATCATCATG GTATGGCTAG CATGACTGGT GGACAGCAAA TGGGTCGGGA TCTGTACGAC 181 GATGACGATA AGGATCTCGC CACCATGGTC GACTCATCAC GTCGTAAGTG GAATAAGACA 241 GGTCACGCAG TCAGAGCTAT AGGTCGGCTG AGCTCACTCG AGAACGTCTA TATCAAGGCC 301 GACAAGCAGA AGAACGGCAT CAAGGCGAAC TTCCACATCC GCCACAACAT CGAGGACGGC 361 GGCGTGCAGC TCGCCTACCA CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG 421 CTGCCCGACA ACCACTACCT GAGCGTGCAG TCCAAACTTT CGAAAGACCC CAACGAGAAG 481 CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC 541 GAGCTGTACA AGGGCGGTAC CATGGTGAGC AAGGGCGAGG Agaataacat ggccatcatc 601 aaggagttca tgcgcttcaa ggtgcgcatg gagggctccg tgaacggcca cgagttcgag 661 atcgagggcg agggcgaggg ccgcccctac gagggctttc agaccgttaa gctgaaggtg 721 accaagggtg gccccctgcc cttcgcctgg gacatcttgt cccctcagtt cacctacggc 781 tccaaggcct acgtgaagca ccccgccgac atccccgact acctcaagct gtccttcccc 841 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgact 901 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac 961 ttcccctccg acggccccgt aatgcagaag aagaccatgg gcatggaggc ctcctccgag 1021 cggatgtacc ccgaggacgg cgccctgaag ggcgaggaca agctcaggct gaagctgaag 1081 gacggcggcc actacacctc cgaggtcaag accacctaca aggccaagaa gcccgtgcag 1141 ttgcccggcg cctacatcgt cgacatcaag ttggacatca cctcccacaa cgaggactac 1201 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggCGG CATGGACGAG 1261 CTGTACAAGG GAGGGAGCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC 1321 ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGT 1381 GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG 1441 CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC 1501 TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACATC 1561 CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG 1621 TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC 1681 GGCAACATCC TGGGGCACAA GCTGGAGTAC AACCTGCCGG ACCAACTGAC TGAAGAGCAG 1741 ATCGCAGAAT TTAAAGAGGC TTTCTCCCTA TTTGACAAGG ACGGGGATGG GACAATAACA 1801 ACCAAGGAGC TGGGGACGGT GATGCGGTCT CTGGGGCAGA ACCCCACAGA AGCAGAGCTG 1861 CAGGACATGA TCAATGAAGT AGATGCCGAC GGTGACGGCA CAATCGACTT CCCTGAGTTC 1921 CTGACAATGA TGGCAAGAAA AATGAAATAC AGGGACACGG AAGAAGAAAT TAGAGAAGCG 1981 TTCGGTGTGT TTGATAAGGA TGGCAATGGC TACATCAGTG CAGCAGAGCT TCGCCACGTG 2041 ATGACAAACC TTGGAGAGAA GTTAACAGAT GAAGAGGTTG ATGAAATGAT CAGGGAAGCA 2101 GACATCGATG GGGATGGTCA GGTAAACTAC GAAGAGTTTG TACAAATGAT GACAGCGAAG 2161 TGAGCGGCCG CGACTCTAGA TCATAATCAG CCATACCACA TTTGTAGAGG TTTTACTTGC 2221 TTTAAAAAAC CTCCCACACC TCCCCCTGAA CCTGAAACAT AAAATGAATG CAATTCGAAG 2281 CTTGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG 2341 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA 2401 GGAGGAACTA TATCCGGATC TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC 2461 AACAGTTGCG CAGCCTGAAT GGCGAATGGG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG 2521 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC 2581 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA 2641 ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC 2701 TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT 2761 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA 2821 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT 2881 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGCTTA 2941 CAATTTAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA 3001 AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA 3061 TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC 3121 GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA 3181 AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT 3241 TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG 3301 TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA 3361 TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT 3421 GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT 3481 ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA 3541 TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA 3601 GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA 3661 ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC 3721 AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC 3781 CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG 3841 TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT 3901 CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA 3961 TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT 4021 TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA 4081 CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG 4141 CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC 4201 AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT 4261 AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC 4321 TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT 4381 GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG 4441 CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT 4501 ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG 4561 GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG 4621 TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG 4681 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG 4741 GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC 4801 CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT 4861 GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT 4921 TCATTAATGC AG //