LOCUS       pRSET_a_-_MatryoshCaMP6 4932 bp ds-DNA     circular     12-JUL-2017
DEFINITION  .
KEYWORDS    "EGFPcp-LSSmO"                                                     
FEATURES             Location/Qualifiers
     misc_feature    169..192
                     /label="Xpress_EK"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1279..1713
                     /label="EGFP (0-431)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(2333..2351)
                     /label="T7 term RV"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             3275..3934
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             2421..2489
                     /label="LacZ alpha"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      4032..4714
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    100..2163
                     /label="ORF frame 1"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    214..276
                     /label="M13 peptide"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    277..282
                     /label="LE linker"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1720..2163
                     /label="CaM"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1714..1719
                     /label="LP linker"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     20..39
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      2508..2814
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    553..561
                     /label="GGT flexible linker"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    100..213
                     /label="pRSET tag"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    283..552
                     /label="EGFP (444-713)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    562..1269
                     /label="LSSmOrange"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1270..1278
                     /label="GGS flexible linker"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    112..129
                     /label="6x HIS tag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    133..165
                     /label="T7 leader/tag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 GATCTCGATC CCGCGAAATT AATACGACTC ACTATAGGGA GACCACAACG GTTTCCCTCT
       61 AGAAATAATT TTGTTTAACT TTAAGAAGGA GATATACATA TGCGGGGTTC TCATCATCAT
      121 CATCATCATG GTATGGCTAG CATGACTGGT GGACAGCAAA TGGGTCGGGA TCTGTACGAC
      181 GATGACGATA AGGATCTCGC CACCATGGTC GACTCATCAC GTCGTAAGTG GAATAAGACA
      241 GGTCACGCAG TCAGAGCTAT AGGTCGGCTG AGCTCACTCG AGAACGTCTA TATCAAGGCC
      301 GACAAGCAGA AGAACGGCAT CAAGGCGAAC TTCCACATCC GCCACAACAT CGAGGACGGC
      361 GGCGTGCAGC TCGCCTACCA CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG
      421 CTGCCCGACA ACCACTACCT GAGCGTGCAG TCCAAACTTT CGAAAGACCC CAACGAGAAG
      481 CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC
      541 GAGCTGTACA AGGGCGGTAC CATGGTGAGC AAGGGCGAGG Agaataacat ggccatcatc
      601 aaggagttca tgcgcttcaa ggtgcgcatg gagggctccg tgaacggcca cgagttcgag
      661 atcgagggcg agggcgaggg ccgcccctac gagggctttc agaccgttaa gctgaaggtg
      721 accaagggtg gccccctgcc cttcgcctgg gacatcttgt cccctcagtt cacctacggc
      781 tccaaggcct acgtgaagca ccccgccgac atccccgact acctcaagct gtccttcccc
      841 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgact
      901 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
      961 ttcccctccg acggccccgt aatgcagaag aagaccatgg gcatggaggc ctcctccgag
     1021 cggatgtacc ccgaggacgg cgccctgaag ggcgaggaca agctcaggct gaagctgaag
     1081 gacggcggcc actacacctc cgaggtcaag accacctaca aggccaagaa gcccgtgcag
     1141 ttgcccggcg cctacatcgt cgacatcaag ttggacatca cctcccacaa cgaggactac
     1201 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggCGG CATGGACGAG
     1261 CTGTACAAGG GAGGGAGCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC
     1321 ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGT
     1381 GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG
     1441 CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC
     1501 TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACATC
     1561 CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG
     1621 TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC
     1681 GGCAACATCC TGGGGCACAA GCTGGAGTAC AACCTGCCGG ACCAACTGAC TGAAGAGCAG
     1741 ATCGCAGAAT TTAAAGAGGC TTTCTCCCTA TTTGACAAGG ACGGGGATGG GACAATAACA
     1801 ACCAAGGAGC TGGGGACGGT GATGCGGTCT CTGGGGCAGA ACCCCACAGA AGCAGAGCTG
     1861 CAGGACATGA TCAATGAAGT AGATGCCGAC GGTGACGGCA CAATCGACTT CCCTGAGTTC
     1921 CTGACAATGA TGGCAAGAAA AATGAAATAC AGGGACACGG AAGAAGAAAT TAGAGAAGCG
     1981 TTCGGTGTGT TTGATAAGGA TGGCAATGGC TACATCAGTG CAGCAGAGCT TCGCCACGTG
     2041 ATGACAAACC TTGGAGAGAA GTTAACAGAT GAAGAGGTTG ATGAAATGAT CAGGGAAGCA
     2101 GACATCGATG GGGATGGTCA GGTAAACTAC GAAGAGTTTG TACAAATGAT GACAGCGAAG
     2161 TGAGCGGCCG CGACTCTAGA TCATAATCAG CCATACCACA TTTGTAGAGG TTTTACTTGC
     2221 TTTAAAAAAC CTCCCACACC TCCCCCTGAA CCTGAAACAT AAAATGAATG CAATTCGAAG
     2281 CTTGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG
     2341 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA
     2401 GGAGGAACTA TATCCGGATC TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC
     2461 AACAGTTGCG CAGCCTGAAT GGCGAATGGG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG
     2521 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC
     2581 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA
     2641 ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC
     2701 TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT
     2761 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA
     2821 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT
     2881 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGCTTA
     2941 CAATTTAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA
     3001 AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA
     3061 TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC
     3121 GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA
     3181 AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT
     3241 TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG
     3301 TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA
     3361 TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT
     3421 GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT
     3481 ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA
     3541 TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA
     3601 GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA
     3661 ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC
     3721 AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC
     3781 CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG
     3841 TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT
     3901 CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA
     3961 TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT
     4021 TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA
     4081 CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG
     4141 CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC
     4201 AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT
     4261 AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC
     4321 TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT
     4381 GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG
     4441 CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT
     4501 ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG
     4561 GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG
     4621 TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG
     4681 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG
     4741 GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC
     4801 CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT
     4861 GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT
     4921 TCATTAATGC AG
//