LOCUS       pRSET_a_-_sfMatyroshCaM 4926 bp ds-DNA     circular     12-JUL-2017
DEFINITION  .
KEYWORDS    "sfGFPcp-LSSmO"                                                    
FEATURES             Location/Qualifiers
     misc_feature    1276..1707
                     /label="sfcpGFP 1st half"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    214..276
                     /label="M13 peptide"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    283..549
                     /label="sfcpGFP 2nd half"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    100..2157
                     /label="ORF frame 1"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    112..129
                     /label="6x HIS tag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(2327..2345)
                     /label="T7 term RV "
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     20..39
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    100..213
                     /label="pRSET tag"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    277..282
                     /label="LE linker"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    559..1266
                     /label="LSSmOrange"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             3269..3928
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             2415..2483
                     /label="LacZ alpha"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      4026..4708
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      2502..2808
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    550..558
                     /label="GGT flexible linker"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1267..1275
                     /label="GGS flexible linker"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1708..1713
                     /label="LP linker"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1714..2157
                     /label="CaM"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    169..192
                     /label="Xpress_EK"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    133..165
                     /label="T7 leader/tag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 GATCTCGATC CCGCGAAATT AATACGACTC ACTATAGGGA GACCACAACG GTTTCCCTCT
       61 AGAAATAATT TTGTTTAACT TTAAGAAGGA GATATACATA TGCGGGGTTC TCATCATCAT
      121 CATCATCATG GTATGGCTAG CATGACTGGT GGACAGCAAA TGGGTCGGGA TCTGTACGAC
      181 GATGACGATA AGGATCTCGC CACCATGGTC GACTCATCAC GTCGTAAGTG GAATAAGACA
      241 GGTCACGCAG TCAGAGCTAT AGGTCGGCTG AGCTCACTCG AGAACGTGTA TATTACCGCG
      301 GATAAACAGA AAAACGGCAT TAAAGCGAAC TTTACCGTGC GCCATAACGT GGAAGATGGC
      361 AGCGTGCAGC TGGCGGATCA TTATCAGCAG AACACCCCGA TTGGCGATGG CCCGGTGCTG
      421 CTGCCGGATA ACCATTATCT GAGCACCCAG ACCAAGCTGA GCAAAGATCC GAACGAAAAA
      481 CGCGATCACA TGGTGCTGCT GGAATTTGTG ACCGCAGCGG GCATTACACA CGGCATGGAT
      541 GAACTGTATG GCGGCACCat ggtgagcaag ggcgaggaga ataacatggc catcatcaag
      601 gagttcatgc gcttcaaggt gcgcatggag ggctccgtga acggccacga gttcgagatc
      661 gagggcgagg gcgagggccg cccctacgag ggctttcaga ccgttaagct gaaggtgacc
      721 aagggtggcc ccctgccctt cgcctgggac atcttgtccc ctcagttcac ctacggctcc
      781 aaggcctacg tgaagcaccc cgccgacatc cccgactacc tcaagctgtc cttccccgag
      841 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgactcag
      901 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
      961 ccctccgacg gccccgtaat gcagaagaag accatgggca tggaggcctc ctccgagcgg
     1021 atgtaccccg aggacggcgc cctgaagggc gaggacaagc tcaggctgaa gctgaaggac
     1081 ggcggccact acacctccga ggtcaagacc acctacaagg ccaagaagcc cgtgcagttg
     1141 cccggcgcct acatcgtcga catcaagttg gacatcacct cccacaacga ggactacacc
     1201 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     1261 tacaagGGCG GCAGCGCGAG CCAGGGCGAA GAACTGTTTA CCGGCGTGGT GCCGATTCTG
     1321 GTGGAACTGG ATGGCGATGT GAACGGCCAT AAATTTAGCG TGCGCGGCGA AGGCGAAGGC
     1381 GATGCGACCA TTGGCAAACT GACCCTGAAA TTTATTTCCA CCACCGGCAA ACTACCGGTG
     1441 CCGTGGCCGA CCCTGGTGAC CACCTTAACC TATGGCGTGC AGTGCTTTAG CCGCTATCCG
     1501 GATCATATGA AACGCCATGA TTTTTTTAAA AGCGCGATGC CGGAAGGCTA TGTGCAGGAA
     1561 CGCACCATTA GCTTTAAAGA TGATGGCAAA TATAAAACCC GCGCGGTGGT GAAATTTGAA
     1621 GGCGATACCC TGGTGAACCG CATTGAACTG AAAGGCACCG ATTTTAAAGA AGATGGCAAC
     1681 ATTCTGGGGC ATAAACTGGA ATATAACCTG CCGGACCAAC TGACTGAAGA GCAGATCGCA
     1741 GAATTTAAAG AGGCTTTCTC CCTATTTGAC AAGGACGGGG ATGGGACAAT AACAACCAAG
     1801 GAGCTGGGGA CGGTGATGCG GTCTCTGGGG CAGAACCCCA CAGAAGCAGA GCTGCAGGAC
     1861 ATGATCAATG AAGTAGATGC CGACGGTGAC GGCACAATCG ACTTCCCTGA GTTCCTGACA
     1921 ATGATGGCAA GAAAAATGAA ATACAGGGAC ACGGAAGAAG AAATTAGAGA AGCGTTCGGT
     1981 GTGTTTGATA AGGATGGCAA TGGCTACATC AGTGCAGCAG AGCTTCGCCA CGTGATGACA
     2041 AACCTTGGAG AGAAGTTAAC AGATGAAGAG GTTGATGAAA TGATCAGGGA AGCAGACATC
     2101 GATGGGGATG GTCAGGTAAA CTACGAAGAG TTTGTACAAA TGATGACAGC GAAGTGAGCG
     2161 GCCGCGACTC TAGATCATAA TCAGCCATAC CACATTTGTA GAGGTTTTAC TTGCTTTAAA
     2221 AAACCTCCCA CACCTCCCCC TGAACCTGAA ACATAAAATG AATGCAATTC GAAGCTTGAT
     2281 CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA
     2341 CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA
     2401 ACTATATCCG GATCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT
     2461 TGCGCAGCCT GAATGGCGAA TGGGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG
     2521 TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG
     2581 CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG
     2641 GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT
     2701 AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT
     2761 TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA
     2821 TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA
     2881 ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG CTTACAATTT
     2941 AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA
     3001 TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA
     3061 AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT
     3121 TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA
     3181 GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG
     3241 TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC
     3301 GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA
     3361 GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT
     3421 AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT
     3481 GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT
     3541 AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA
     3601 CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT
     3661 TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC
     3721 ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA
     3781 GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT
     3841 AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA
     3901 GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT
     3961 TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA
     4021 TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT
     4081 AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA
     4141 AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT
     4201 TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA
     4261 GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT
     4321 AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC
     4381 AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA
     4441 GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA
     4501 AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG
     4561 AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT
     4621 CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG
     4681 CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT
     4741 TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT
     4801 TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA
     4861 GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA
     4921 ATGCAG
//