LOCUS       pRSET_a_-_sfGCaMP6s-T78 4218 bp ds-DNA     circular     13-JUL-2017
DEFINITION  .
KEYWORDS    "sfGFPcp"                                                          
FEATURES             Location/Qualifiers
     misc_feature    214..276
                     /label="M13 peptide"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     rep_origin      1794..2100
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    100..1449
                     /label="ORF frame 1"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     rep_origin      3318..4000
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     20..39
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1000..1005
                     /label="LP linker"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1006..1449
                     /label="CaM"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    112..129
                     /label="6x HIS tag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    100..213
                     /label="pRSET tag"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    169..192
                     /label="Xpress_EK"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    283..999
                     /label="sfcpGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             2561..3220
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    277..282
                     /label="LE linker"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             1707..1775
                     /label="LacZ alpha"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    133..165
                     /label="T7 leader/tag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    334..336
                     /label="T78H"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
ORIGIN
        1 GATCTCGATC CCGCGAAATT AATACGACTC ACTATAGGGA GACCACAACG GTTTCCCTCT
       61 AGAAATAATT TTGTTTAACT TTAAGAAGGA GATATACATA TGCGGGGTTC TCATCATCAT
      121 CATCATCATG GTATGGCTAG CATGACTGGT GGACAGCAAA TGGGTCGGGA TCTGTACGAC
      181 GATGACGATA AGGATCTCGC CACCATGGTC GACTCATCAC GTCGTAAGTG GAATAAGACA
      241 GGTCACGCAG TCAGAGCTAT AGGTCGGCTG AGCTCACTCG AGAACGTGTA TATTACCGCG
      301 GATAAACAGA AAAACGGCAT TAAAGCGAAC TTTCACGTGC GCCATAACGT GGAAGATGGC
      361 AGCGTGCAGC TGGCGGATCA TTATCAGCAG AACACCCCGA TTGGCGATGG CCCGGTGCTG
      421 CTGCCGGATA ACCATTATCT GAGCACCCAG ACCAAGCTGA GCAAAGATCC GAACGAAAAA
      481 CGCGATCACA TGGTGCTGCT GGAATTTGTG ACCGCAGCGG GCATTACACA CGGCATGGAT
      541 GAACTGTATG GCGGCACCGG CGGCAGCGCG AGCCAGGGCG AAGAACTGTT TACCGGCGTG
      601 GTGCCGATTC TGGTGGAACT GGATGGCGAT GTGAACGGCC ATAAATTTAG CGTGCGCGGC
      661 GAAGGCGAAG GCGATGCGAC CATTGGCAAA CTGACCCTGA AATTTATTTC CACCACCGGC
      721 AAACTACCGG TGCCGTGGCC GACCCTGGTG ACCACCTTAA CCTATGGCGT GCAGTGCTTT
      781 AGCCGCTATC CGGATCATAT GAAACGCCAT GATTTTTTTA AAAGCGCGAT GCCGGAAGGC
      841 TATGTGCAGG AACGCACCAT TAGCTTTAAA GATGATGGCA AATATAAAAC CCGCGCGGTG
      901 GTGAAATTTG AAGGCGATAC CCTGGTGAAC CGCATTGAAC TGAAAGGCAC CGATTTTAAA
      961 GAAGATGGCA ACATTCTGGG GCATAAACTG GAATATAACC TGCCGGACCA ACTGACTGAA
     1021 GAGCAGATCG CAGAATTTAA AGAGGCTTTC TCCCTATTTG ACAAGGACGG GGATGGGACA
     1081 ATAACAACCA AGGAGCTGGG GACGGTGATG CGGTCTCTGG GGCAGAACCC CACAGAAGCA
     1141 GAGCTGCAGG ACATGATCAA TGAAGTAGAT GCCGACGGTG ACGGCACAAT CGACTTCCCT
     1201 GAGTTCCTGA CAATGATGGC AAGAAAAATG AAATACAGGG ACACGGAAGA AGAAATTAGA
     1261 GAAGCGTTCG GTGTGTTTGA TAAGGATGGC AATGGCTACA TCAGTGCAGC AGAGCTTCGC
     1321 CACGTGATGA CAAACCTTGG AGAGAAGTTA ACAGATGAAG AGGTTGATGA AATGATCAGG
     1381 GAAGCAGACA TCGATGGGGA TGGTCAGGTA AACTACGAAG AGTTTGTACA AATGATGACA
     1441 GCGAAGTGAG CGGCCGCGAC TCTAGATCAT AATCAGCCAT ACCACATTTG TAGAGGTTTT
     1501 ACTTGCTTTA AAAAACCTCC CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT
     1561 TCGAAGCTTG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC
     1621 GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG
     1681 CTGAAAGGAG GAACTATATC CGGATCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC
     1741 CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGGACGC GCCCTGTAGC GGCGCATTAA
     1801 GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC
     1861 CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG
     1921 CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA
     1981 AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC
     2041 GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA
     2101 CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT
     2161 ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA
     2221 CGCTTACAAT TTAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT
     2281 TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA
     2341 ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT
     2401 TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA
     2461 TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA
     2521 GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT
     2581 GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT
     2641 ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA
     2701 TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC
     2761 CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT
     2821 GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA
     2881 CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC
     2941 TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA
     3001 AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC
     3061 TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC
     3121 CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG
     3181 ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA
     3241 CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA
     3301 GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC
     3361 GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT
     3421 CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA
     3481 GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT
     3541 TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA
     3601 CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC
     3661 CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG
     3721 TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG
     3781 TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG
     3841 CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT
     3901 TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC
     3961 AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT
     4021 TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG
     4081 TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA
     4141 GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG
     4201 GCCGATTCAT TAATGCAG
//