LOCUS       pAAV.CAG.Flex.Twitch2B.WPRE.SV40\(Addgene\49531M)\\p3409\Q        6638 bp    DNA     circular     14-APR-2014
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|694863574|
COMMENT     VNTDBDATE|695222473|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV.CAG.Flex.Twitch2B.WPRE.SV40 (Addgene 49531M)  p3409 Q|
COMMENT     VNTAUTHORNAME|Audrey Mazza|
COMMENT     VNTOAUTHORNAME|Demo User|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pAAV.CAG.Flex.Twitch2B.WPRE.SV40 (Addgene 49531M)  p3409 Q" 0 
COMMENT       6638 
COMMENT       (CDBMol 0 0 1 3692 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "ApaLI" "AvaI" "BamHI" "ClaI" "EcoRI" "HindIII" "NcoI" 
COMMENT         "PstI" "SmaI" "XmaI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 6638 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 3 3293 0 4617 0 5863 0 0 
COMMENT            "") 
COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 9 31 0 42 0 944 0 1373 0 
COMMENT            2009 0 2948 0 3687 0 4083 0 4094 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 4 2061 0 3079 0 3674 0 3931 
COMMENT            0 0 "") #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 1901 0 3072 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 437 0 1616 0 2932 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 5 33 0 44 0 3689 0 4085 0 
COMMENT            4096 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 5 31 0 42 0 3687 0 4083 0 
COMMENT            4094 0 0 "")) 
COMMENT       (CObList 
COMMENT        #10=(CFSignal (CObList) "loxP" 21 0 1 3037 3070 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3041..3074)") 
COMMENT        #11=(CFSignal (CObList) "loxP2272" 21 0 1 2953 2986 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2957..2990)") 
COMMENT        #12=(CFSignal (CObList) "loxP" 21 0 0 1218 1251 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1222..1255") 
COMMENT        #13=(CFSignal (CObList) "loxP2272" 21 0 0 1134 1167 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1138..1171") 
COMMENT        #14=(CFSignal (CObList) "CAG (CBA) Promoter" 29 0 0 192 1126 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "194..1130") 
COMMENT        #15=(CFSignal (CObList) "ITR" 34 0 0 1 188 0 (CStringList) (CStringList) 
COMMENT             1 1 1 1 "1..190") 
COMMENT        #16=(CFSignal (CObList) "ITR" 34 0 1 3938 4127 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3942..4131)") 
COMMENT        #17=(CFSignal (CObList) "AmpR" 21 0 0 5122 5982 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5126..5986") 
COMMENT        #18=(CFSignal (CObList) "pBr322 origin" 21 0 0 4348 4967 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4352..4971") 
COMMENT        #19=(CFSignal (CObList) "WPRE" 21 0 0 3085 3672 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3089..3676") 
COMMENT        #20=(CFSignal (CObList) "Twitch2B" 4 0 1 1267 2934 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1271..2938)") 
COMMENT        #21=(CFSignal (CObList) "sv40" 25 0 0 3693 3931 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3697..3935")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #22=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #23=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) 
COMMENT                    "pAAV.CAG.Flex.Twitch2B.WPRE.SV40 (Addgene 49531M)  p3409 Q" 
COMMENT                    2) 5 "" 0 4) 
COMMENT              #24=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA 'pAAV.CAG.Flex.Twitch2B.WPRE.SV40 (Addgene 49531M)  p3409 Q'" 
COMMENT                          1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: Demo User" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 04/10/14 09:39AM" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 04/14/14 01:21PM" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6638 bp" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #32=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 10-APR-2014" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #35=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 5 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Demo User" 
COMMENT                          1))) "Original Author") 
COMMENT              #37=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #38=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) 
COMMENT                   "Annotations") 
COMMENT              #39=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #40=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #41=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1267 (3 #20# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #42=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1267  End: 2934 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #43=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #44=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(1271..2938)" 1))) 
COMMENT                                 "Twitch2B"))) "CDS (1 total)") 
COMMENT                     #45=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #46=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1134 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #47=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1134  End: 1167" 1) 
COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #49=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1138..1171" 1))) "loxP2272") 
COMMENT                            #50=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1218 (3 #12# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1218  End: 1251" 1) 
COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1222..1255" 1))) "loxP") 
COMMENT                            #54=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2953 (3 #11# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2953  End: 2986 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2957..2990)" 1))) 
COMMENT                                 "loxP2272") 
COMMENT                            #58=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3037 (3 #10# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3037  End: 3070 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(3041..3074)" 1))) "loxP") 
COMMENT                            #62=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3085 (3 #19# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3085  End: 3672" 1) 
COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3089..3676" 1))) "WPRE") 
COMMENT                            #66=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4348 (3 #18# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4348  End: 4967" 1) 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4352..4971" 1))) "pBr322 origin") 
COMMENT                            #70=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5122 (3 #17# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5122  End: 5982" 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5126..5986" 1))) "AmpR"))) 
COMMENT                          "Misc. Feature (7 total)") 
COMMENT                     #74=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 25 (7 25 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #75=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3693 (3 #21# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3693  End: 3931" 1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3697..3935" 1))) "sv40"))) 
COMMENT                          "PolyA Signal (1 total)") 
COMMENT                     #79=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #80=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 192 (3 #14# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 192   End: 1126" 1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   194..1130" 1))) "CAG (CBA) Promoter"))) 
COMMENT                          "Promoter Eukaryotic (1 total)") 
COMMENT                     #84=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 34 (7 34 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #85=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1 (3 #15# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1     End: 188 " 1) 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1..190" 1))) "ITR") 
COMMENT                            #89=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3938 (3 #16# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3938  End: 4127 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(3942..4131)" 1))) "ITR"))) 
COMMENT                          "Repeat Region (2 total)"))) "Feature Map") 
COMMENT              #93=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #94=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 58279384 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #95=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #0# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #96=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 3293 " 1) 
COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #0# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 4617 " 1) 
COMMENT                                   #98=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #0# 3) 1 2 2 0 191) 
COMMENT                                        " N3: 5863 " 1))))) "ApaLI: 3 sites") 1 
COMMENT                          5 "GTGCAC" "CACGTG") 
COMMENT                     #99=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 58279512 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #100=(CGroupPar 
COMMENT                                   (CParagraph 0 (10 #1# 0) 1 2 2 0 180) 
COMMENT                                   (CObjectList 
COMMENT                                    #101=(CLinePar 
COMMENT                                          (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                          " N1: 31 " 1) 
COMMENT                                    #102=(CLinePar 
COMMENT                                          (CParagraph 0 (1 #1# 2) 1 2 2 0 191) 
COMMENT                                          " N2: 42 " 1) 
COMMENT                                    #103=(CLinePar 
COMMENT                                          (CParagraph 0 (1 #1# 3) 1 2 2 0 191) 
COMMENT                                          " N3: 944 " 1) 
COMMENT                                    #104=(CLinePar 
COMMENT                                          (CParagraph 0 (1 #1# 4) 1 2 2 0 191) 
COMMENT                                          " N4: 1373 " 1) 
COMMENT                                    #105=(CLinePar 
COMMENT                                          (CParagraph 0 (1 #1# 5) 1 2 2 0 191) 
COMMENT                                          " N5: 2009 " 1) 
COMMENT                                    #106=(CLinePar 
COMMENT                                          (CParagraph 0 (1 #1# 6) 1 2 2 0 191) 
COMMENT                                          " N6: 2948 " 1) 
COMMENT                                    #107=(CLinePar 
COMMENT                                          (CParagraph 0 (1 #1# 7) 1 2 2 0 191) 
COMMENT                                          " N7: 3687 " 1) 
COMMENT                                    #108=(CLinePar 
COMMENT                                          (CParagraph 0 (1 #1# 8) 1 2 2 0 191) 
COMMENT                                          " N8: 4083 " 1) 
COMMENT                                    #109=(CLinePar 
COMMENT                                          (CParagraph 0 (1 #1# 9) 1 2 2 0 191) 
COMMENT                                          " N9: 4094 " 1))))) "AvaI: 9 sites") 1 
COMMENT                          5 "CYCGRG" "GRGCYC") 
COMMENT                     #110=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 58275160 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList)) "BamHI: 0 sites") 1 5 "GGATCC" 
COMMENT                           "CCTAGG") 
COMMENT                     #111=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 58275352 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #112=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #3# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #113=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 2061 " 1) 
COMMENT                                     #114=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #3# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 3079 " 1) 
COMMENT                                     #115=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #3# 3) 1 2 2 0 191) 
COMMENT                                           " N3: 3674 " 1) 
COMMENT                                     #116=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #3# 4) 1 2 2 0 191) 
COMMENT                                           " N4: 3931 " 1))))) "ClaI: 4 sites") 
COMMENT                           2 4 "ATCGAT" "TAGCTA") 
COMMENT                     #117=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 58275416 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList)) "EcoRI: 0 sites") 1 5 "GAATTC" 
COMMENT                           "CTTAAG") 
COMMENT                     #118=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 58278680 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #119=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #5# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #120=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #5# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 1901 " 1) 
COMMENT                                     #121=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #5# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 3072 " 1))))) 
COMMENT                            "HindIII: 2 sites") 1 5 "AAGCTT" "TTCGAA") 
COMMENT                     #122=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 58278776 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #123=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #6# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #124=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #6# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 437 " 1) 
COMMENT                                     #125=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #6# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 1616 " 1) 
COMMENT                                     #126=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #6# 3) 1 2 2 0 191) 
COMMENT                                           " N3: 2932 " 1))))) "NcoI: 3 sites") 
COMMENT                           1 5 "CCATGG" "GGTACC") 
COMMENT                     #127=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 58278840 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList)) "PstI: 0 sites") 5 1 "CTGCAG" 
COMMENT                           "GACGTC") 
COMMENT                     #128=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 58278904 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #129=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #8# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #130=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #8# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 33 " 1) 
COMMENT                                     #131=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #8# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 44 " 1) 
COMMENT                                     #132=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #8# 3) 1 2 2 0 191) 
COMMENT                                           " N3: 3689 " 1) 
COMMENT                                     #133=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #8# 4) 1 2 2 0 191) 
COMMENT                                           " N4: 4085 " 1) 
COMMENT                                     #134=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #8# 5) 1 2 2 0 191) 
COMMENT                                           " N5: 4096 " 1))))) "SmaI: 5 sites") 
COMMENT                           3 3 "CCCGGG" "GGGCCC") 
COMMENT                     #135=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 58279032 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #136=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #9# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #137=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #9# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 31 " 1) 
COMMENT                                     #138=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #9# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 42 " 1) 
COMMENT                                     #139=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #9# 3) 1 2 2 0 191) 
COMMENT                                           " N3: 3687 " 1) 
COMMENT                                     #140=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #9# 4) 1 2 2 0 191) 
COMMENT                                           " N4: 4083 " 1) 
COMMENT                                     #141=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #9# 5) 1 2 2 0 191) 
COMMENT                                           " N5: 4094 " 1))))) "XmaI: 5 sites") 
COMMENT                           1 5 "CCCGGG" "GGGCCC"))) 
COMMENT                   "Restriction/Methylation Map")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #142=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 
COMMENT                "@N (@S)" 0) 
COMMENT          #143=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #144=(CWidgetStyle "Molecule Label 2" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #145=(CWidgetStyle "Molecule Label 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #146=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 
COMMENT                (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #147=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 
COMMENT                (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #148=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 
COMMENT                (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) 
COMMENT          #149=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #150=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #151=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #152=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #153=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #154=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #155=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 
COMMENT                1 65535 "@N (@H)" 0) 
COMMENT          #156=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #157=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
COMMENT          #158=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #159=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT                0 0) 
COMMENT          #160=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) 
COMMENT          #161=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 
COMMENT                (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) 
COMMENT          #162=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #163=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #164=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #165=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #166=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #167=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #168=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #169=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #170=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #171=(CWidgetStyle "Annotation" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                0 0) 
COMMENT          #172=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #173=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #174=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #175=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 65535 "@N" 0) 
COMMENT          #176=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 
COMMENT                "@N" 0) 
COMMENT          #177=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 3 1 
COMMENT                (LOGBRUSH 0 16777113 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 
COMMENT                (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #178=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 
COMMENT                1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #179=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 
COMMENT                1 1 "@N" 0) 
COMMENT          #180=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #181=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 
COMMENT                "@N (@S)" 0) 
COMMENT          #182=(CWidgetStyle "Label 0" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 1.05556 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #183=(CWidgetStyle "Shape 7" 1 (LOGPEN 0 0 65331) 1 1 
COMMENT                (LOGBRUSH 0 3407718 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #184=(CWidgetStyle "Shape 8" 1 (LOGPEN 0 0 255) 1 1 (LOGBRUSH 0 255 0) 
COMMENT                1 (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 
COMMENT                (LOGSHAPE 9 1 0.8 1.8 0))) 0.164644 1.74233 0.164644 2.53336 
COMMENT         (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 
COMMENT          (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 -365) 
COMMENT        1 1 0 1 1 
COMMENT        (mapper: 20.0914 -19.6519 40.0378 40.0378 0.01 10 -1 6638 6638 1 0 0) 
COMMENT        #185=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 745434985 100) 
COMMENT              (CObjectList 
COMMENT               #186=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -799 100) 
COMMENT                     (CObjectList 
COMMENT                      #187=(CAxis 
COMMENT                            (CWideLine 
COMMENT                             (CWidget 0 (0 0) 1 2 0 0 #148# 58029244 0) 
COMMENT                             (LOGPEN 0 0 10079436) 2 (LOGBRUSH 0 13434879 0) 1 
COMMENT                             6.27513 6.27313 1 0.0214037) 0.0945041) 
COMMENT                      #188=(CLabel 
COMMENT                            (CWidget 1001 (0 0) 1 2 0 0 #145# 4289900 100) 
COMMENT                            (LOGPEN 0 0 13408563) 1 
COMMENT                            (LOGFONT 58 22 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 
COMMENT                            1.74233 0.833333 0 
COMMENT                            "pAAV.CAG.Flex.Twitch2B.WPRE.SV40 (Addgene 49531M)  p3409 Q" 
COMMENT                            "@N" 1 0 0.871165 0 -10 36.6903 1.34873 Nil) 
COMMENT                      #189=(CLabel (CWidget 1002 (0 0) 1 2 0 0 #144# 0 100) 
COMMENT                            (LOGPEN 0 0 13408563) 1 
COMMENT                            (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 
COMMENT                            1.74233 0.555556 0 "6638 bp" "@L bp" 16 0 -0.871165 
COMMENT                            0 -10.8 2.62252 0.974079 Nil)) (CObjectList)) 
COMMENT               #190=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -1427 100) 
COMMENT                     (CObjectList 
COMMENT                      #191=(CGroupWidget 
COMMENT                            (CWidget 4 (7 4 0) 1 2 0 0 Nil -799 100) 
COMMENT                            (CObjectList 
COMMENT                             #192=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #20# 0) 1 2 0 0 #146# 0 100) 
COMMENT                                    (LOGPEN 0 0 3355545) 1 
COMMENT                                    (LOGBRUSH 0 6724095 0) 1 3.50043 5.07676 1 
COMMENT                                    0.082322) 0.8 1.8 1) 
COMMENT                             #193=(CLabel (CWidget 0 (0 0) 1 2 0 0 #143# 80 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "Twitch2B" "@N" 
COMMENT                                   1 13.7121 -12.1963 0 -10 3.5966 1.12394 #192#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #194=(CGroupWidget 
COMMENT                            (CWidget 21 (7 21 0) 1 2 0 0 Nil -1497 100) 
COMMENT                            (CObjectList 
COMMENT                             #195=(CScratch 
COMMENT                                   (CWidget 0 (3 #10# 0) 1 2 0 0 #151# 83373156 
COMMENT                                    100) (LOGPEN 0 8 13395507) 10 1 3.40404 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #196=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #143# 10550 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "loxP" "@N" 1 
COMMENT                                   5.19008 -19.1656 0 -10 1.72337 1.12394 #195#) 
COMMENT                             #197=(CScratch 
COMMENT                                   (CWidget 0 (3 #11# 0) 1 2 0 0 #151# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 3.48342 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #198=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #143# 892350832 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "loxP2272" "@N" 
COMMENT                                   1 7.03001 -17.4233 0 -10 3.47172 1.12394 
COMMENT                                   #197#) 
COMMENT                             #199=(CScratch 
COMMENT                                   (CWidget 0 (3 #12# 0) 1 2 0 0 #151# 83374688 
COMMENT                                    100) (LOGPEN 0 8 13395507) 10 1 5.12307 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #200=(CLabel (CWidget 0 (0 0) 1 2 0 0 #143# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "loxP" "@N" 1 
COMMENT                                   12.8491 -1.74233 0 -10 1.72337 1.12394 #199#) 
COMMENT                             #201=(CScratch 
COMMENT                                   (CWidget 0 (3 #13# 0) 1 2 0 0 #151# 
COMMENT                                    1504793081 100) (LOGPEN 0 8 13395507) 10 1 
COMMENT                                   5.20245 1.9 0.082322 1) 
COMMENT                             #202=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #143# 10544 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "loxP2272" "@N" 
COMMENT                                   1 13.9358 0 0 -10 3.47172 1.12394 #201#) 
COMMENT                             #203=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #17# 0) 1 2 0 0 #147# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 0.619947 1.43363 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #204=(CLabel (CWidget 0 (0 0) 1 2 0 0 #143# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "AmpR" "@N" 1 
COMMENT                                   -11.9333 6.96932 0 -10 2.42271 1.12394 #203#) 
COMMENT                             #205=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #18# 0) 1 2 0 0 #147# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 1.57916 2.16509 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #206=(CLabel (CWidget 0 (0 0) 1 2 0 0 #143# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "pBr322 origin" 
COMMENT                                   "@N" 1 -14.5888 5.22699 0 -10 5.17011 1.12394 
COMMENT                                   #205#) 
COMMENT                             #207=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #19# 0) 1 2 0 0 #147# 444 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 2.80299 
COMMENT                                    3.35868 1 0.082322) 0.8 1.8 0) 
COMMENT                             #208=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #143# 1635218031 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "WPRE" "@N" 1 
COMMENT                                   -3.18568 -20.908 0 -10 2.42271 1.12394 #207#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #209=(CGroupWidget 
COMMENT                            (CWidget 25 (7 25 0) 1 2 0 0 Nil -381 100) 
COMMENT                            (CObjectList 
COMMENT                             #210=(CLine 
COMMENT                                   (CWidget 0 (3 #21# 0) 1 2 0 0 #162# 543716457 
COMMENT                                    100) (LOGPEN 0 6 32768) 8 0.835356 2.55823 
COMMENT                                   2.78409) 
COMMENT                             #211=(CLabel (CWidget 0 (0 0) 1 2 0 0 #143# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "sv40" "@N" 1 
COMMENT                                   -7.23961 -12.1963 0 -10 1.84825 1.12394 #210#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #212=(CGroupWidget 
COMMENT                            (CWidget 29 (7 29 0) 1 2 0 0 Nil -1218 100) 
COMMENT                            (CObjectList 
COMMENT                             #213=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #14# 0) 1 2 0 0 #147# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 5.20907 
COMMENT                                    6.09268 1 0.082322) 0.8 1.8 0) 
COMMENT                             #214=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #143# 28781 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "CAG (CBA) Promoter" "@N" 1 16.0327 3.48466 0 
COMMENT                                   -10 7.91752 1.12394 #213#)) (CObjectList)) 
COMMENT                      #215=(CGroupWidget 
COMMENT                            (CWidget 34 (7 34 0) 1 2 0 0 Nil -1009 100) 
COMMENT                            (CObjectList 
COMMENT                             #216=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #15# 0) 1 2 0 0 #158# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    6.09552 6.27319 1 0.082322) 0.8 1.8 0) 
COMMENT                             #217=(CLabel (CWidget 0 (0 0) 1 2 0 0 #143# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "ITR" "@N" 1 
COMMENT                                   10.8416 6.96932 0 -10 1.34873 1.12394 #216#) 
COMMENT                             #218=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #16# 0) 1 2 0 0 #158# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    2.373 2.55256 1 0.082322) 0.8 1.8 1) 
COMMENT                             #219=(CLabel (CWidget 0 (0 0) 1 2 0 0 #143# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "ITR" "@N" 1 
COMMENT                                   -9.46838 -8.71165 0 -10 1.34873 1.12394 #218#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #220=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -171 100) 
COMMENT                     (CObjectList 
COMMENT                      #221=(CGroupWidget 
COMMENT                            (CWidget 2 (10 #5# 0) 1 2 0 0 Nil -311 100) 
COMMENT                            (CObjectList 
COMMENT                             #222=(CScratch 
COMMENT                                   (CWidget 1 (1 #5# 1) 1 2 0 0 #150# 83374836 
COMMENT                                    100) (LOGPEN 0 6 10053171) 8 1 4.47761 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #223=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #142# 942682480 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Hin}dIII (1901)" "@N (@S)" 5 14.8929 
COMMENT                                   -6.96932 0 -10 4.29594 0.974079 #222#) 
COMMENT                             #224=(CScratch 
COMMENT                                   (CWidget 2 (1 #5# 2) 1 2 0 0 #150# 59059480 
COMMENT                                    100) (LOGPEN 0 6 10053171) 8 1 3.37096 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #225=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #142# 942683424 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Hin}dIII (3072)" "@N (@S)" 5 6.86272 
COMMENT                                   -20.908 0 -10 4.42082 0.974079 #224#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #226=(CGroupWidget 
COMMENT                            (CWidget 3 (10 #0# 0) 1 2 0 0 Nil -869 100) 
COMMENT                            (CObjectList 
COMMENT                             #227=(CScratch 
COMMENT                                   (CWidget 1 (1 #0# 1) 1 2 0 0 #150# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 3.16211 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #228=(CLabel (CWidget 0 (0 0) 1 2 0 0 #142# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Apa}LI (3293)" "@N (@S)" 5 4.10992 
COMMENT                                   -24.3926 0 -10 4.04618 0.974079 #227#) 
COMMENT                             #229=(CScratch 
COMMENT                                   (CWidget 2 (1 #0# 2) 1 2 0 0 #150# 1504793201 
COMMENT                                    100) (LOGPEN 0 6 10053171) 8 1 1.91087 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #230=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #142# 4289900 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Apa}LI (4617)" "@N (@S)" 5 -14.2381 
COMMENT                                   3.48466 0 -10 3.99622 0.974079 #229#) 
COMMENT                             #231=(CScratch 
COMMENT                                   (CWidget 3 (1 #0# 3) 1 2 0 0 #150# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 0.733352 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #232=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #142# 539570745 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Apa}LI (5863)" "@N (@S)" 5 -11.3937 
COMMENT                                   8.71165 0 -10 4.04618 0.974079 #231#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #233=(CGroupWidget 
COMMENT                            (CWidget 3 (10 #6# 0) 1 2 0 0 Nil -102 100) 
COMMENT                            (CObjectList 
COMMENT                             #234=(CScratch 
COMMENT                                   (CWidget 1 (1 #6# 1) 1 2 0 0 #150# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 5.86115 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #235=(CLabel (CWidget 0 (0 0) 1 2 0 0 #142# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Nco}I (437)" "@N (@S)" 5 13.1696 
COMMENT                                   5.22699 0 -10 3.29688 0.974079 #234#) 
COMMENT                             #236=(CScratch 
COMMENT                                   (CWidget 2 (1 #6# 2) 1 2 0 0 #150# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 4.74694 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #237=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #142# 10553 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Nco}I (1616)" "@N (@S)" 5 14.5202 
COMMENT                                   -5.22699 0 -10 3.54665 0.974079 #236#) 
COMMENT                             #238=(CScratch 
COMMENT                                   (CWidget 3 (1 #6# 3) 1 2 0 0 #150# 83373636 
COMMENT                                    100) (LOGPEN 0 6 10053171) 8 1 3.50327 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #239=(CLabel (CWidget 0 (0 0) 1 2 0 0 #142# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Nco}I (2932)" "@N (@S)" 5 7.5323 
COMMENT                                   -13.9386 0 -10 3.67153 0.974079 #238#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #240=(CGroupWidget 
COMMENT                            (CWidget 4 (10 #3# 0) 1 2 0 0 Nil -1497 100) 
COMMENT                            (CObjectList 
COMMENT                             #241=(CScratch 
COMMENT                                   (CWidget 1 (1 #3# 1) 1 2 0 0 #150# 5726 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 4.3264 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #242=(CLabel (CWidget 0 (0 0) 1 2 0 0 #142# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Cla}I (2061)" "@N (@S)" 5 13.9909 
COMMENT                                   -10.454 0 -10 3.37181 0.974079 #241#) 
COMMENT                             #243=(CScratch 
COMMENT                                   (CWidget 2 (1 #3# 2) 1 2 0 0 #150# 454 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 3.36435 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #244=(CLabel (CWidget 0 (0 0) 1 2 0 0 #142# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Cla}I (3079)" "@N (@S)" 5 6.1294 
COMMENT                                   -22.6503 0 -10 3.42177 0.974079 #243#) 
COMMENT                             #245=(CScratch 
COMMENT                                   (CWidget 3 (1 #3# 3) 1 2 0 0 #150# 83373008 
COMMENT                                    100) (LOGPEN 0 6 10053171) 8 1 2.80205 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #246=(CLabel (CWidget 0 (0 0) 1 2 0 0 #142# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Cla}I (3674)" "@N (@S)" 5 -7.37841 
COMMENT                                   -19.1656 0 -10 3.42177 0.974079 #245#) 
COMMENT                             #247=(CScratch 
COMMENT                                   (CWidget 4 (1 #3# 4) 1 2 0 0 #150# 813 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 2.55917 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #248=(CLabel (CWidget 0 (0 0) 1 2 0 0 #142# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Cla}I (3931)" "@N (@S)" 5 -9.44861 
COMMENT                                   -10.454 0 -10 3.37181 0.974079 #247#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #249=(CGroupWidget 
COMMENT                            (CWidget 5 (10 #8# 0) 1 2 0 0 Nil -730 100) 
COMMENT                            (CObjectList 
COMMENT                             #250=(CScratch 
COMMENT                                   (CWidget 1 (1 #8# 1) 1 2 0 0 #150# 59108288 
COMMENT                                    100) (LOGPEN 0 6 10053171) 8 1 6.24294 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #251=(CLabel (CWidget 0 (0 0) 1 2 0 0 #142# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Sma}I (33)" "@N (@S)" 5 3.34099 13.9386 
COMMENT                                   0 -10 3.09707 0.974079 #250#) 
COMMENT                             #252=(CScratch 
COMMENT                                   (CWidget 2 (1 #8# 2) 1 2 0 0 #150# 1504792929 
COMMENT                                    100) (LOGPEN 0 6 10053171) 8 1 6.23255 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #253=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #142# -1497 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Sma}I (44)" "@N (@S)" 5 10.6793 8.71165 
COMMENT                                   0 -10 3.09707 0.974079 #252#) 
COMMENT                             #254=(CScratch 
COMMENT                                   (CWidget 3 (1 #8# 3) 1 2 0 0 #150# 84613912 
COMMENT                                    100) (LOGPEN 0 6 10053171) 8 1 2.78787 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #255=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #142# 7208984 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Sma}I (3689)" "@N (@S)" 5 -7.50404 
COMMENT                                   -13.9386 0 -10 3.84637 0.974079 #254#) 
COMMENT                             #256=(CScratch 
COMMENT                                   (CWidget 4 (1 #8# 4) 1 2 0 0 #150# 84615512 
COMMENT                                    100) (LOGPEN 0 6 10053171) 8 1 2.41364 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #257=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #142# 2081693732 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Sma}I (4085)" "@N (@S)" 5 -14.0465 
COMMENT                                   -3.48466 0 -10 3.84637 0.974079 #256#) 
COMMENT                             #258=(CScratch 
COMMENT                                   (CWidget 5 (1 #8# 5) 1 2 0 0 #150# 511 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 2.40324 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #259=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #142# 57687520 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Sma}I (4096)" "@N (@S)" 5 -14.365 
COMMENT                                   1.74233 0 -10 3.84637 0.974079 #258#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #260=(CGroupWidget 
COMMENT                            (CWidget 5 (10 #9# 0) 1 2 0 0 Nil -939 100) 
COMMENT                            (CObjectList 
COMMENT                             #261=(CScratch 
COMMENT                                   (CWidget 1 (1 #9# 1) 1 2 0 0 #150# 84613512 
COMMENT                                    100) (LOGPEN 0 6 10053171) 8 1 6.24483 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #262=(CLabel (CWidget 0 (0 0) 1 2 0 0 #142# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Xma}I (31)" "@N (@S)" 5 3.51834 17.4233 
COMMENT                                   0 -10 3.04712 0.974079 #261#) 
COMMENT                             #263=(CScratch 
COMMENT                                   (CWidget 2 (1 #9# 2) 1 2 0 0 #150# 511 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 6.23444 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #264=(CLabel (CWidget 0 (0 0) 1 2 0 0 #142# 10 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Xma}I (42)" "@N (@S)" 5 3.38802 12.1963 
COMMENT                                   0 -10 3.09707 0.974079 #263#) 
COMMENT                             #265=(CScratch 
COMMENT                                   (CWidget 3 (1 #9# 3) 1 2 0 0 #150# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 2.78976 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #266=(CLabel (CWidget 0 (0 0) 1 2 0 0 #142# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Xma}I (3687)" "@N (@S)" 5 -7.73503 
COMMENT                                   -17.4233 0 -10 3.84637 0.974079 #265#) 
COMMENT                             #267=(CScratch 
COMMENT                                   (CWidget 4 (1 #9# 4) 1 2 0 0 #150# 1209 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 2.41553 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #268=(CLabel (CWidget 0 (0 0) 1 2 0 0 #142# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Xma}I (4083)" "@N (@S)" 5 -12.6362 
COMMENT                                   -6.96932 0 -10 3.84637 0.974079 #267#) 
COMMENT                             #269=(CScratch 
COMMENT                                   (CWidget 5 (1 #9# 5) 1 2 0 0 #150# 367 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 2.40513 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #270=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #142# -1260 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Xma}I (4094)" "@N (@S)" 5 -14.426 
COMMENT                                   -1.74233 0 -10 3.84637 0.974079 #269#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #271=(CGroupWidget 
COMMENT                            (CWidget 9 (10 #1# 0) 1 2 0 0 Nil -1078 100) 
COMMENT                            (CObjectList 
COMMENT                             #272=(CScratch 
COMMENT                                   (CWidget 1 (1 #1# 1) 1 2 0 0 #150# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 6.24483 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #273=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #142# 1090533425 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Ava}I (31)" "@N (@S)" 5 3.35968 15.681 
COMMENT                                   0 -10 2.87229 0.974079 #272#) 
COMMENT                             #274=(CScratch 
COMMENT                                   (CWidget 2 (1 #1# 2) 1 2 0 0 #150# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 6.23444 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #275=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #142# 1818324585 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Ava}I (42)" "@N (@S)" 5 8.42491 10.454 
COMMENT                                   0 -10 2.92224 0.974079 #274#) 
COMMENT                             #276=(CScratch 
COMMENT                                   (CWidget 3 (1 #1# 3) 1 2 0 0 #150# 1297638611 
COMMENT                                    100) (LOGPEN 0 6 10053171) 8 1 5.38201 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #277=(CLabel (CWidget 0 (0 0) 1 2 0 0 #142# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Ava}I (944)" "@N (@S)" 5 14.0688 
COMMENT                                   1.74233 0 -10 3.29688 0.974079 #276#) 
COMMENT                             #278=(CScratch 
COMMENT                                   (CWidget 4 (1 #1# 4) 1 2 0 0 #150# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 4.97659 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #279=(CLabel (CWidget 0 (0 0) 1 2 0 0 #142# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Ava}I (1373)" "@N (@S)" 5 14.0969 
COMMENT                                   -3.48466 0 -10 3.62158 0.974079 #278#) 
COMMENT                             #280=(CScratch 
COMMENT                                   (CWidget 5 (1 #1# 5) 1 2 0 0 #150# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 4.37554 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #281=(CLabel (CWidget 0 (0 0) 1 2 0 0 #142# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Ava}I (2009)" "@N (@S)" 5 14.296 
COMMENT                                   -8.71165 0 -10 3.67153 0.974079 #280#) 
COMMENT                             #282=(CScratch 
COMMENT                                   (CWidget 6 (1 #1# 6) 1 2 0 0 #150# 83372796 
COMMENT                                    100) (LOGPEN 0 6 10053171) 8 1 3.48815 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #283=(CLabel (CWidget 0 (0 0) 1 2 0 0 #142# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Ava}I (2948)" "@N (@S)" 5 7.49088 
COMMENT                                   -15.681 0 -10 3.67153 0.974079 #282#) 
COMMENT                             #284=(CScratch 
COMMENT                                   (CWidget 7 (1 #1# 7) 1 2 0 0 #150# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 2.78976 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #285=(CLabel (CWidget 0 (0 0) 1 2 0 0 #142# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Ava}I (3687)" "@N (@S)" 5 -7.57637 
COMMENT                                   -15.681 0 -10 3.67153 0.974079 #284#) 
COMMENT                             #286=(CScratch 
COMMENT                                   (CWidget 8 (1 #1# 8) 1 2 0 0 #150# 83373848 
COMMENT                                    100) (LOGPEN 0 6 10053171) 8 1 2.41553 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #287=(CLabel (CWidget 0 (0 0) 1 2 0 0 #142# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Ava}I (4083)" "@N (@S)" 5 -13.4484 
COMMENT                                   -5.22699 0 -10 3.67153 0.974079 #286#) 
COMMENT                             #288=(CScratch 
COMMENT                                   (CWidget 9 (1 #1# 9) 1 2 0 0 #150# 83373996 
COMMENT                                    100) (LOGPEN 0 6 10053171) 8 1 2.40513 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #289=(CLabel (CWidget 0 (0 0) 1 2 0 0 #142# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Ava}I (4094)" "@N (@S)" 5 -14.4411 0 0 
COMMENT                                   -10 3.67153 0.974079 #288#)) (CObjectList))) 
COMMENT                     (CObjectList)) 
COMMENT               #290=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil 776294992 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #291=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 776294992 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 
COMMENT       -2147483648 (CStringList) 2147483648 268632118 (CObList)))
FEATURES             Location/Qualifiers
     misc_feature    complement(3037..3070)
                     /vntifkey="21"
                     /label=loxP
                     /note="loxP"
     misc_feature    complement(2953..2986)
                     /vntifkey="21"
                     /label=loxP2272
                     /note="loxP2272"
     misc_feature    1218..1251
                     /vntifkey="21"
                     /label=loxP
                     /note="loxP"
     misc_feature    1134..1167
                     /vntifkey="21"
                     /label=loxP2272
                     /note="loxP2272"
     promoter        192..1126
                     /vntifkey="29"
                     /label=CAG\(CBA)\Promoter
     repeat_region   1..188
                     /vntifkey="34"
                     /label=ITR
     repeat_region   complement(3938..4127)
                     /vntifkey="34"
                     /label=ITR
     misc_feature    5122..5982
                     /vntifkey="21"
                     /label=AmpR
     misc_feature    4348..4967
                     /vntifkey="21"
                     /label=pBr322\origin
     misc_feature    3085..3672
                     /vntifkey="21"
                     /label=WPRE
     CDS             complement(1267..2934)
                     /vntifkey="4"
                     /label=Twitch2B
     polyA_signal    3693..3931
                     /vntifkey="25"
                     /label=sv40
BASE COUNT     1422 a      1747 c      1772 g      1697 t 
ORIGIN
        1 gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt 
       61 tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac 
      121 taggggttcc ttgtagttaa tgattaaccc gccatgctac ttatctacgt agccatgctc 
      181 taggaagagt accattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 
      241 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 
      301 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 
      361 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 
      421 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct 
      481 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga 
      541 tgggggcggg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg 
      601 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc 
      661 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg 
      721 agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc 
      781 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 
      841 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag 
      901 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggc tgtccgcggg 
      961 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg 
     1021 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg 
     1081 gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattggat cttataactt 
     1141 cgtataggat actttatacg aagttatgca gaatggtagc tggattgtag ctgctattag 
     1201 caatatgaaa cctcttaata acttcgtata gcatacatta tacgaagtta tactagttga 
     1261 tcatcaatcc tcaatgttgt gacggatctt aaaattagcc ttaatgccgt tcttttgctt 
     1321 atcggctgtg atgtaaacat tgtgactgtt gtagttgtat tccaacttat gcccgagtat 
     1381 gtttccgtcc tccttaaagt cgattccctt cagctcgatt cggttgacaa gtgtatcccc 
     1441 ttcgaatttc acctcggccc tagtcttgta attaccatcg tccttgaaga agatagttct 
     1501 ctcctggacg tacccctctg gcatggctga tttaaagaaa tcgtgttgtt tcatatggtc 
     1561 tgggtagcgg gcgaaacaca taaggccata tcctagagta gtaacgagtg tgggccatgg 
     1621 cacgggcagt ttccccgttg tgcagatgag cttgagtgtc aatttgccat tagttgcgtc 
     1681 cccctcaccc tcccctcgca cgctgaattt atgaccgttg acatccccat ccagttccac 
     1741 caaaataggg actacaccag tgaacaattc ttcgcccttg ctcaccatag atcccccggt 
     1801 cccacctttg tacagctcgt ccattccgag agtgattccg gcggcggtga caaattccag 
     1861 caggaccatg tgatcccgct tttcattagg atctttggaa agcttggact ggtatgacag 
     1921 gtagtggttg tcaggcagga gaaccggccc atccccgatc ggagtattct gctggtagtg 
     1981 atctgcaagc tggaccccac ccatgagctc ggggtagatg ggctgcacgt tttccaccat 
     2041 cttcaggaac tcgtcgaaat cgattctgcc gttcttgtcg gtgtcggagt cgccgaagat 
     2101 ctcgtccacg tcctcgtcgg tcagctgctc gccggtcagt ctgatgatgt cgccaaactc 
     2161 ctctctgtcg atgaagccgt tgccgtcgaa gtcgaagatt ctgaagcact cgctcagttc 
     2221 ctcttcgctg gcgtcggcaa cttgcatgcg ggcggcggtc acgaactcca gcaggaccat 
     2281 gtgatcgcgc ttctcgttgg ggtctttgct cagcttggac tgggtgctca ggtagtggtt 
     2341 gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag 
     2401 ctgcacgctg ccgtcctcga tgttgcagtt gaggccgaag ttggccttga tgccgttctt 
     2461 ctgcttgtcg gcggtgatat agacgttgcc gtggatggcg ttgtactcca gcttgtgccc 
     2521 caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt 
     2581 gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat 
     2641 ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat 
     2701 gtggtcgggg tagcgggcga agcactgcac gccccagctc agggtggtca cgagggtggg 
     2761 ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt 
     2821 ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag 
     2881 ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatggtggc 
     2941 gctagcctcg agataacttc gtataaagta tcctatacga agttatttgc cttaacccag 
     3001 aaattatcac tgttattctt tagaatggtg caaagaataa cttcgtataa tgtatgctat 
     3061 acgaagttat aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac 
     3121 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt 
     3181 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt 
     3241 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt 
     3301 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg 
     3361 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg 
     3421 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc 
     3481 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt 
     3541 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc 
     3601 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc 
     3661 cgcctccccg catcgatacc gtcgacccgg gcggccgctt cgagcagaca tgataagata 
     3721 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga 
     3781 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa 
     3841 caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaaag 
     3901 caagtaaaac ctctacaaat gtggtaaaat cgataaggat cttcctagag catggctacg 
     3961 tagataagta gcatggcggg ttaatcatta actacaagga acccctagtg atggagttgg 
     4021 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac 
     4081 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc attaatgaat 
     4141 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 
     4201 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 
     4261 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 
     4321 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 
     4381 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 
     4441 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 
     4501 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 
     4561 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 
     4621 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 
     4681 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 
     4741 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 
     4801 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 
     4861 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 
     4921 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 
     4981 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 
     5041 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 
     5101 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 
     5161 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 
     5221 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 
     5281 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 
     5341 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 
     5401 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 
     5461 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 
     5521 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 
     5581 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 
     5641 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 
     5701 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 
     5761 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 
     5821 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 
     5881 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 
     5941 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 
     6001 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 
     6061 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta 
     6121 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac 
     6181 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg 
     6241 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa 
     6301 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt 
     6361 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt 
     6421 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga 
     6481 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc 
     6541 gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg 
     6601 gaagggcgat cggtgcgggc ctcttcgcta ttacgcca 
//
