LOCUS       pAAV.synP.DIO.EGFP.WPRE.hGH\(p2629)\Q        5520 bp    DNA     circular     30-AUG-2013
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|671703377|
COMMENT     VNTDBDATE|671717811|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV.synP.DIO.EGFP.WPRE.hGH (p2629) Q|
COMMENT     VNTAUTHORNAME|Shu-Jen Chen|
COMMENT     VNTOAUTHORNAME|Mitesh Mehta|
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FEATURES             Location/Qualifiers
     polyA_signal    2265..2743
                     /vntifkey="25"
                     /label=hGH\polyA
     LTR             1..130
                     /vntifkey="19"
                     /label=L-ITR
     LTR             complement(2787..2923)
                     /vntifkey="19"
                     /label=R-ITR
     CDS             complement(775..1494)
                     /vntifkey="4"
                     /label=EGFP
     CDS             3840..4697
                     /vntifkey="4"
                     /label=AmpR
     misc_feature    1637..2245
                     /vntifkey="21"
                     /label=WPRE
     promoter        145..621
                     /vntifkey="29"
                     /label=synapsin-1\promoter
     misc_feature    730..763
                     /vntifkey="21"
                     /label=loxP
     misc_feature    complement(1589..1622)
                     /vntifkey="21"
                     /label=loxP
     misc_feature    1505..1538
                     /vntifkey="21"
                     /label=loxP2272
     misc_feature    complement(646..679)
                     /vntifkey="21"
                     /label=loxP2272
BASE COUNT     1133 a      1513 c      1489 g      1385 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct gcggccgcac gcgtgctaga ctgcagaggg ccctgcgtat gagtgcaagt 
      181 gggttttagg accaggatga ggcggggtgg gggtgcctac ctgacgaccg accccgaccc 
      241 actggacaag cacccaaccc ccattcccca aattgcgcat cccctatcag agagggggag 
      301 gggaaacagg atgcggcgag gcgcgtgcgc actgccagct tcagcaccgc ggacagtgcc 
      361 ttcgcccccg cctggcggcg cgcgccaccg ccgcctcagc actgaaggcg cgctgacgtc 
      421 actcgccggt cccccgcaaa ctccccttcc cggccacctt ggtcgcgtcc gcgccgccgc 
      481 cggcccagcc ggaccgcacc acgcgaggcg cgagataggg gggcacgggc gcgaccatct 
      541 gcgctgcggc gccggcgact cagcgctgcc tcagtctgcg gtgggcagcg gaggagtcgt 
      601 gtcgtgcctg agagcgcagt cgaattctct agagtcgact ccggaataac ttcgtatagg 
      661 atactttata cgaagttatg cagaatggta gctggattgt agctgctatt agcaatatga 
      721 aacctcttaa taacttcgta tagcatacat tatacgaagt tatggcgcgc cctattactt 
      781 gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat 
      841 gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac tgggtgctca ggtagtggtt 
      901 gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag 
      961 ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga tgccgttctt 
     1021 ctgcttgtcg gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc 
     1081 caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt 
     1141 gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat 
     1201 ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat 
     1261 gtggtcgggg tagcggctga agcactgcac gccgtaggtc agggtggtca cgagggtggg 
     1321 ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt 
     1381 ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag 
     1441 ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatggtggc 
     1501 tagcataact tcgtataaag tatcctatac gaagttattt gccttaaccc agaaattatc 
     1561 actgttattc tttagaatgg tgcaaagaat aacttcgtat aatgtatgct atacgaagtt 
     1621 atgaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat 
     1681 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 
     1741 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 
     1801 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 
     1861 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 
     1921 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 
     1981 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 
     2041 aatcatcgtc ctttccttgg ctgctcgcct atgttgccac ctggattctg cgcgggacgt 
     2101 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 
     2161 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 
     2221 gggccgcctc cccgcatcga taccgagcgc tgctcgagag atctacgggt ggcatccctg 
     2281 tgacccctcc ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct 
     2341 tgtcctaata aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatattat 
     2401 ggggtggagg ggggtggtat ggagcaaggg gcaagttggg aagacaacct gtagggcctg 
     2461 cggggtctat tgggaaccaa gctggagtgc agtggcacaa tcttggctca ctgcaatctc 
     2521 cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc gagttgttgg gattccaggc 
     2581 atgcatgacc aggctcagct aatttttgtt tttttggtag agacggggtt tcaccatatt 
     2641 ggccaggctg gtctccaact cctaatctca ggtgatctac ccaccttggc ctcccaaatt 
     2701 gctgggatta caggcgtgaa ccactgctcc cttccctgtc cttctgattt tgtaggtaac 
     2761 cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg 
     2821 cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc 
     2881 cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat 
     2941 tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg 
     3001 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 
     3061 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 
     3121 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg 
     3181 ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat 
     3241 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 
     3301 tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag 
     3361 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 
     3421 cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct 
     3481 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac 
     3541 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca 
     3601 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac 
     3661 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt 
     3721 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt 
     3781 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta 
     3841 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg 
     3901 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac 
     3961 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg 
     4021 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc 
     4081 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg 
     4141 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat 
     4201 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg 
     4261 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg 
     4321 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc 
     4381 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt 
     4441 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct 
     4501 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc 
     4561 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca 
     4621 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct 
     4681 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt 
     4741 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 
     4801 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 
     4861 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 
     4921 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 
     4981 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag 
     5041 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 
     5101 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 
     5161 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 
     5221 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc 
     5281 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 
     5341 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 
     5401 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 
     5461 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 
//
