LOCUS Exported 3644 bp ds-DNA circular SYN 30-JUN-2017 DEFINITION . ACCESSION . VERSION . KEYWORDS pYPQ131D2.0 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3644) AUTHORS . TITLE Direct Submission JOURNAL Exported Friday, Jun 30, 2017 from SnapGene 3.3.4 http://www.snapgene.com COMMENT LOCUS pHD1 3087 bp DNA circular 10-DEC-2010 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|567775241| COMMENT VNTDBDATE|573229794| COMMENT LSOWNER| COMMENT VNTNAME|pHD1| COMMENT VNTAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc ""pHD1"" 0 3087 COMMENT (CDBMol 0 0 1 1 1 0 0 0 0 """" """" 0 0 0 0 (CObList) (CObList) (CObList) COMMENT (CObList) -1 """") COMMENT (CDocSetData 1 1 0 0 0 0 ""MAIN"" 1 1 1 1 0 0 1 1 1 0 10 10 4294967295 50 0 COMMENT 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) COMMENT (CStringList ""ApaLI"" ""AvaI"" ""BamHI"" ""ClaI"" ""EcoRI"" ""HindIII"" ""NcoI"" COMMENT ""PstI"" ""SmaI"" ""XmaI"" ""XbaI"" ""BsaI"") (CStringList ""atg"" ""gtg"") COMMENT (CStringList ""taa"" ""tga"" ""tag"") (CObList) 1 ""{(0,1),2}"" 0 0 """" 0 COMMENT 4294967295 0 0 0 0 0 0 0 ""MAIN"" 0 0 30 0 COMMENT (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) COMMENT (CMolPar 1 0 0 0 0 1 3087 0 0 0 0 0 0 0 0) (CStringList) (CStringList) COMMENT (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList #0=(CRSite (CStringList) ""ApaLI"" ""gtgcac"" 2 0 1 2267 0 0 """") COMMENT #1=(CRSite (CStringList) ""AvaI"" ""cycgrg"" 2 0 1 1847 0 0 """") COMMENT #2=(CRSite (CStringList) ""BamHI"" ""ggatcc"" 2 0 0 0 """") COMMENT #3=(CRSite (CStringList) ""ClaI"" ""atcgat"" 3 0 1 211 0 0 """") COMMENT #4=(CRSite (CStringList) ""EcoRI"" ""gaattc"" 2 0 0 0 """") COMMENT #5=(CRSite (CStringList) ""HindIII"" ""aagctt"" 2 0 0 0 """") COMMENT #6=(CRSite (CStringList) ""NcoI"" ""ccatgg"" 2 0 1 1835 0 0 """") COMMENT #7=(CRSite (CStringList) ""PstI"" ""ctgcag"" 6 0 0 0 """") COMMENT #8=(CRSite (CStringList) ""SmaI"" ""cccggg"" 4 0 0 0 """") COMMENT #9=(CRSite (CStringList) ""XmaI"" ""cccggg"" 2 0 0 0 """") COMMENT #10=(CRSite (CStringList) ""XbaI"" ""tctaga"" 2 0 1 1749 0 0 """") COMMENT #11=(CRSite (CStringList) ""BsaI"" ""ggtctc"" 8 12 2 1761 0 1835 1 0 """")) COMMENT (CObList COMMENT #12=(CFSignal (CObList) ""TetR"" 21 0 0 272 1462 0 (CStringList) COMMENT (CStringList) 1 1 1 1 """") COMMENT #13=(CFSignal (CObList) ""Tet_promoter"" 30 0 0 229 235 0 (CStringList) COMMENT (CStringList) 1 1 1 1 """") COMMENT #14=(CFSignal (CObList) ""pTC14_F1 primer"" 28 0 0 2979 2998 0 COMMENT (CStringList) (CStringList) 1 1 1 1 """") COMMENT #15=(CFSignal (CObList) ""end"" 21 0 0 1633 1633 0 (CStringList) COMMENT (CStringList) 1 1 1 1 """") COMMENT #16=(CFSignal (CObList) ""pTC14_R1 primer"" 28 0 0 1703 1724 0 COMMENT (CStringList) (CStringList) 1 1 1 1 """") COMMENT #17=(CFSignal (CObList) ""start"" 21 0 0 1 1 0 (CStringList) (CStringList) COMMENT 1 1 1 1 """") COMMENT #18=(CFSignal (CObList) ""pTC14_F2 primer"" 28 0 0 1607 1626 0 COMMENT (CStringList) (CStringList) 1 1 1 1 """") COMMENT #19=(CFSignal (CObList) ""HD1"" 21 0 0 1754 1845 0 (CStringList) COMMENT (CStringList) 1 1 1 1 """") COMMENT #20=(CFSignal (CObList) ""ori"" 21 0 0 1910 2583 0 (CStringList) COMMENT (CStringList) 1 1 1 1 """") COMMENT #21=(CFSignal (CObList) ""HD1 frag"" 21 0 0 1765 1838 0 (CStringList) COMMENT (CStringList) 1 1 1 1 """")) (CObList) (CObList) (CObList) (CObList) COMMENT (CObList) (CObList) COMMENT (CTextView 0 COMMENT #22=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #23=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) ""pHD1"" 2) 5 """" 0 4) COMMENT #24=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) ""DNA 'pHD1'"" COMMENT 1) COMMENT #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT ""Local object"" 1) COMMENT #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT ""Created: 10/11/10 11:20AM"" 1) COMMENT #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT ""Last Modified: 12/10/10 02:29PM"" 1) COMMENT #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT ""length: 3087 bp"" 1) COMMENT #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT ""storage type: Basic"" 1) COMMENT #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT ""form: Circular"" 1))) ""General Description"") COMMENT #32=(CFolderPar COMMENT (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) (CObjectList)) COMMENT ""Standard Fields"") COMMENT #33=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) ""Comments"" 2) 1 """" COMMENT 0 0) COMMENT #34=(CFolderPar COMMENT (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) COMMENT ""Annotations"") COMMENT #35=(CFolderPar COMMENT (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #36=(CFolderPar COMMENT (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #37=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1 (3 #17# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #38=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT ""Start: 1 End: 1 "" 1))) ""start"") COMMENT #39=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 272 (3 #12# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #40=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT ""Start: 272 End: 1462"" 1))) ""TetR"") COMMENT #41=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1633 (3 #15# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #42=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT ""Start: 1633 End: 1633"" 1))) ""end"") COMMENT #43=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1754 (3 #19# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #44=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT ""Start: 1754 End: 1845"" 1))) ""HD1"") COMMENT #45=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1765 (3 #21# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #46=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT ""Start: 1765 End: 1838"" 1))) ""HD1 frag"") COMMENT #47=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1910 (3 #20# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #48=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT ""Start: 1910 End: 2583"" 1))) ""ori""))) COMMENT ""Misc. Feature (6 total)"") COMMENT #49=(CFolderPar COMMENT (CGroupPar (CParagraph 28 (7 28 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #50=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1607 (3 #18# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #51=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT ""Start: 1607 End: 1626"" 1))) COMMENT ""pTC14_F2 primer"") COMMENT #52=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1703 (3 #16# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #53=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT ""Start: 1703 End: 1724"" 1))) COMMENT ""pTC14_R1 primer"") COMMENT #54=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2979 (3 #14# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #55=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT ""Start: 2979 End: 2998"" 1))) COMMENT ""pTC14_F1 primer""))) COMMENT ""Primer Binding Site (3 total)"") COMMENT #56=(CFolderPar COMMENT (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #57=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 229 (3 #13# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #58=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT ""Start: 229 End: 235 "" 1))) COMMENT ""Tet_promoter""))) COMMENT ""Promoter Prokaryotic (1 total)""))) ""Feature Map"") COMMENT #59=(CFolderPar COMMENT (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #60=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 50460432 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #61=(CGroupPar COMMENT (CParagraph 0 (10 #0# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #62=(CLinePar COMMENT (CParagraph 0 (1 #0# 1) 1 2 2 0 191) COMMENT "" N1: 2267 "" 1))))) ""ApaLI: 1 site"") 1 5 COMMENT ""GTGCAC"" ""CACGTG"") COMMENT #63=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 50460464 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #64=(CGroupPar COMMENT (CParagraph 0 (10 #1# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #65=(CLinePar COMMENT (CParagraph 0 (1 #1# 1) 1 2 2 0 191) COMMENT "" N1: 1847 "" 1))))) ""AvaI: 1 site"") 1 5 COMMENT ""CYCGRG"" ""GRGCYC"") COMMENT #66=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 50460496 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) ""BamHI: 0 sites"") 1 5 ""GGATCC"" COMMENT ""CCTAGG"") COMMENT #67=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 75270552 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #68=(CGroupPar COMMENT (CParagraph 0 (10 #11# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #69=(CLinePar COMMENT (CParagraph 0 (1 #11# 1) 1 2 2 0 191) COMMENT "" N1: 1761 "" 1) COMMENT #70=(CLinePar COMMENT (CParagraph 0 (1 #11# 2) 1 2 2 0 191) COMMENT "" N2: 1835 (on complementary strand)"" 1))))) COMMENT ""BsaI: 2 sites"") 7 11 ""GGTCTCNNNNNN"" ""CCAGAGNNNNNN"") COMMENT #71=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 50460528 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #72=(CGroupPar COMMENT (CParagraph 0 (10 #3# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #73=(CLinePar COMMENT (CParagraph 0 (1 #3# 1) 1 2 2 0 191) COMMENT "" N1: 211 "" 1))))) ""ClaI: 1 site"") 2 4 COMMENT ""ATCGAT"" ""TAGCTA"") COMMENT #74=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 50460624 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) ""EcoRI: 0 sites"") 1 5 ""GAATTC"" COMMENT ""CTTAAG"") COMMENT #75=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 50460656 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) ""HindIII: 0 sites"") 1 5 ""AAGCTT"" COMMENT ""TTCGAA"") COMMENT #76=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 50460752 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #77=(CGroupPar COMMENT (CParagraph 0 (10 #6# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #78=(CLinePar COMMENT (CParagraph 0 (1 #6# 1) 1 2 2 0 191) COMMENT "" N1: 1835 "" 1))))) ""NcoI: 1 site"") 1 5 COMMENT ""CCATGG"" ""GGTACC"") COMMENT #79=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 50460816 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) ""PstI: 0 sites"") 5 1 ""CTGCAG"" COMMENT ""GACGTC"") COMMENT #80=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 50461008 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) ""SmaI: 0 sites"") 3 3 ""CCCGGG"" COMMENT ""GGGCCC"") COMMENT #81=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 50460400 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #82=(CGroupPar COMMENT (CParagraph 0 (10 #10# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #83=(CLinePar COMMENT (CParagraph 0 (1 #10# 1) 1 2 2 0 191) COMMENT "" N1: 1749 "" 1))))) ""XbaI: 1 site"") 1 5 COMMENT ""TCTAGA"" ""AGATCT"") COMMENT #84=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 50461040 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) ""XmaI: 0 sites"") 1 5 ""CCCGGG"" COMMENT ""GGGCCC""))) ""Restriction/Methylation Map"")))) COMMENT (CGraphView COMMENT (CWStyleSheet COMMENT (CObjectList COMMENT #85=(CWidgetStyle ""RSite Label"" 1 (LOGPEN 0 0 13408563) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 ""Georgia"") 0.555556 0 1 5 COMMENT ""@N (@S)"" 0) COMMENT #86=(CWidgetStyle ""Signal Label"" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 ""Arial"") 0.666667 0 1 1 COMMENT ""@N"" 0) COMMENT #87=(CWidgetStyle ""Molecule Label 2"" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 ""Georgia"") 0.555556 0 1 16 COMMENT ""@L bp"" 0) COMMENT #88=(CWidgetStyle ""Molecule Label 1"" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 ""Verdana"") 0.833333 0 1 1 COMMENT ""@N"" 0) COMMENT #89=(CWidgetStyle ""Shape 3"" 1 (LOGPEN 0 0 3355545) 1 1 COMMENT (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #90=(CWidgetStyle ""Shape 1"" 1 (LOGPEN 0 0 6723840) 1 1 COMMENT (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #91=(CWidgetStyle ""Axis"" 1 (LOGPEN 0 0 10079436) 2 1 COMMENT (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) COMMENT #92=(CWidgetStyle ""Line 2"" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #93=(CWidgetStyle ""RSite"" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #94=(CWidgetStyle ""Short Signal"" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #95=(CWidgetStyle ""Uniq RSite Label"" 1 (LOGPEN 0 0 153) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 ""Georgia"") 0.555556 128 1 5 COMMENT ""@N (@S)"" 0) COMMENT #96=(CWidgetStyle ""Vanilla"" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 ""Times New Roman"") 0.8 0 1 COMMENT 2 ""?"" 0) COMMENT #97=(CWidgetStyle ""Mark 1"" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 ""Windings"") 0.7 0 1 2 ""?"" 0) COMMENT #98=(CWidgetStyle ""Motif Label"" 1 (LOGPEN 0 0 16744512) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 ""Arial"") 0.611111 8388608 1 COMMENT 65535 ""@N (@H)"" 0) COMMENT #99=(CWidgetStyle ""Fragment Label 2"" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 ""Courier New"") 1.05 0 1 48 COMMENT ""@F bp (molecule @L bp)"" 0) COMMENT #100=(CWidgetStyle ""Fragment Label 1"" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 ""Arial"") 0.91 0 1 1 COMMENT ""Fragment of @N"" 0) COMMENT #101=(CWidgetStyle ""Shape 4"" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 2 8388608 5) 0 0 0) COMMENT #102=(CWidgetStyle ""Shape 2"" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 COMMENT 0 0) COMMENT #103=(CWidgetStyle ""Shape 0"" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 COMMENT 0) COMMENT #104=(CWidgetStyle ""ORF"" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 COMMENT (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) COMMENT #105=(CWidgetStyle ""Line 4"" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) COMMENT #106=(CWidgetStyle ""Line 3"" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) COMMENT #107=(CWidgetStyle ""Line 1"" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) COMMENT #108=(CWidgetStyle ""Short Promoter"" 1 (LOGPEN 0 0 128) 6 0 0 0 0) COMMENT #109=(CWidgetStyle ""Motif"" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #110=(CWidgetStyle ""Line 0"" 1 (LOGPEN 0 0 0) 8 0 0 0 0) COMMENT #111=(CWidgetStyle ""Void"" 0 0 0 0 0) COMMENT #112=(CWidgetStyle ""General Label"" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 ""Times New Roman"") 0.91 0 COMMENT 1 3 ""@T @N "" 0) COMMENT #113=(CWidgetStyle ""Position"" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #114=(CWidgetStyle ""Annotation"" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 ""Times New Roman"") 0.91 0 COMMENT 0 0) COMMENT #115=(CWidgetStyle ""Position Label"" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 ""Arial"") 0.63 8388608 1 1 COMMENT ""@N"" 0) COMMENT #116=(CWidgetStyle ""Range"" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 0 0 0) COMMENT #117=(CWidgetStyle ""Range Label"" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 ""Arial"") 0.63 8388608 1 1 COMMENT ""@N"" 0) COMMENT #118=(CWidgetStyle ""ORF Label"" 1 (LOGPEN 0 0 49216) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 ""Times New Roman"") COMMENT 0.611111 0 1 65535 ""@N"" 0) COMMENT #119=(CWidgetStyle ""CDS Label"" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 ""Arial"") 0.555556 255 1 1 COMMENT ""@N"" 0) COMMENT #120=(CWidgetStyle ""Shape 5"" 1 (LOGPEN 0 0 0) 3 1 COMMENT (LOGBRUSH 0 16777113 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 ""Arial"") 0.9 0 0 1 COMMENT (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #121=(CWidgetStyle ""CDS"" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 COMMENT 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #122=(CWidgetStyle ""Label 2"" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 ""Arial"") 0.944444 8388608 COMMENT 1 1 ""@N"" 0) COMMENT #123=(CWidgetStyle ""Label 3"" 1 (LOGPEN 0 0 8421376) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 ""Arial"") 0.833333 255 1 COMMENT 5 ""@N (@S)"" 0) COMMENT #124=(CWidgetStyle ""Label 4"" 1 (LOGPEN 0 0 8437824) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 ""Arial"") 0.722222 0 1 5 COMMENT ""@N (@S)"" 0)) 0.164644 1.74233 0.164644 2.53336 COMMENT (70 (CShapeMapEntry 0 ""Unique RSite"" 1 ""Uniq RSite Label"") 67 COMMENT (CShapeMapEntry 0 ""ORF"" 0 ""ORF Label"")) 40.0378 40.0378 39 39 0.1 COMMENT -3087) 1 1 1 1 1 COMMENT (mapper: 19.947 -16.2255 40.0378 40.0378 0.01 10 -1 3087 3087 1 0 0) COMMENT #125=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 1867275361 100) COMMENT (CObjectList COMMENT #126=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -575 100) COMMENT (CObjectList COMMENT #127=(CAxis COMMENT (CWideLine (CWidget 0 (0 0) 1 2 0 0 #91# 0 0) COMMENT (LOGPEN 0 0 10079436) 2 (LOGBRUSH 0 13434879 0) 1 COMMENT 6.27622 6.27422 1 0.0214037) 0.203213) COMMENT #128=(CLabel (CWidget 1001 (0 0) 1 2 0 0 #88# 0 100) COMMENT (LOGPEN 0 2 13408563) 1 COMMENT (LOGFONT 58 22 0 0 400 0 0 0 0 3 2 1 34 ""Verdana"") COMMENT 1.74233 0.833333 0 ""pHD1"" ""@N"" 1 0 0.871165 0 COMMENT -2.91312 3.09707 1.39868 Nil) COMMENT #129=(CLabel (CWidget 1002 (0 0) 1 2 0 0 #87# 128 100) COMMENT (LOGPEN 0 2 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 ""Georgia"") COMMENT 1.74233 0.555556 0 ""3087 bp"" ""@L bp"" 16 0 -0.871165 COMMENT 0 -4.1798 2.59755 0.799245 Nil)) (CObjectList)) COMMENT #130=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -3801 100) COMMENT (CObjectList COMMENT #131=(CGroupWidget COMMENT (CWidget 21 (7 21 0) 1 2 0 0 Nil -3421 100) COMMENT (CObjectList COMMENT #132=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #12# 0) 1 2 0 0 #90# COMMENT 1667321128 100) (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 3.30221 5.72248 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #133=(CLabel (CWidget 0 (0 0) 1 2 0 0 #86# 183 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT ""Arial"") 1.74233 0.666667 0 ""TetR"" ""@N"" 1 COMMENT 14.2917 -3.48466 -7.45921 3.45752 1.94816 COMMENT 1.04901 #132#) COMMENT #134=(CScratch COMMENT (CWidget 0 (3 #15# 0) 1 2 0 0 #94# 0 100) COMMENT (LOGPEN 0 8 13395507) 10 1 2.95675 1.9 COMMENT 0.082322 1) COMMENT #135=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #86# 2082602632 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT ""Arial"") 1.74233 0.666667 0 ""end"" ""@N"" 1 COMMENT -4.28108 -17.4233 1.41405 16.1243 1.67342 COMMENT 1.04901 #134#) COMMENT #136=(CScratch COMMENT (CWidget 0 (3 #17# 0) 1 2 0 0 #94# 81331368 COMMENT 100) (LOGPEN 0 8 13395507) 10 1 6.27319 1.9 COMMENT 0.082322 1) COMMENT #137=(CLabel (CWidget 0 (0 0) 1 2 0 0 #86# 1 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT ""Arial"") 1.74233 0.666667 0 ""start"" ""@N"" 1 COMMENT 1.92479 13.9386 -15.1076 3.45752 2.04806 COMMENT 1.04901 #136#) COMMENT #138=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #19# 0) 1 2 0 0 #90# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 2.52391 2.71086 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #139=(CLabel (CWidget 0 (0 0) 1 2 0 0 #86# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT ""Arial"") 1.74233 0.666667 0 ""HD1"" ""@N"" 1 COMMENT -9.94678 -10.454 3.06426 11.0576 1.87323 COMMENT 1.04901 #138#) COMMENT #140=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #20# 0) 1 2 0 0 #90# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 1.02419 2.39385 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #141=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #86# 23855376 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT ""Arial"") 1.74233 0.666667 0 ""ori"" ""@N"" 1 COMMENT -13.9763 -1.74233 7.02313 4.7242 1.24882 COMMENT 1.04901 #140#) COMMENT #142=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #21# 0) 1 2 0 0 #90# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.835356 2.53813 COMMENT 2.68851 1 0.082322) 0.8 1.8 0) COMMENT #143=(CLabel (CWidget 0 (0 0) 1 2 0 0 #86# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT ""Arial"") 1.74233 0.666667 0 ""HD1 frag"" ""@N"" COMMENT 1 -12.5422 -8.71165 3.76243 9.79092 3.79641 COMMENT 1.04901 #142#)) (CObjectList)) COMMENT #144=(CGroupWidget COMMENT (CWidget 28 (7 28 0) 1 2 0 0 Nil -2663 100) COMMENT (CObjectList COMMENT #145=(CScratch COMMENT (CWidget 0 (3 #14# 0) 1 2 0 0 #107# 0 100) COMMENT (LOGPEN 0 0 16711680) 1 1 0.221502 1.9 COMMENT 0.082322 1) COMMENT #146=(CLabel (CWidget 0 (0 0) 1 2 0 0 #86# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT ""Arial"") 1.74233 0.666667 0 COMMENT ""pTC14 F1 primer"" ""@N"" 1 -7.24676 13.9386 COMMENT 15.8887 3.45752 7.14325 1.04901 #145#) COMMENT #147=(CScratch COMMENT (CWidget 0 (3 #16# 0) 1 2 0 0 #107# 0 100) COMMENT (LOGPEN 0 0 16711680) 1 1 2.8145 1.9 0.082322 COMMENT 1) COMMENT #148=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #86# 50138704 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT ""Arial"") 1.74233 0.666667 0 COMMENT ""pTC14 R1 primer"" ""@N"" 1 -8.76391 -15.681 COMMENT 4.01685 14.8576 7.24316 1.04901 #147#) COMMENT #149=(CScratch COMMENT (CWidget 0 (3 #18# 0) 1 2 0 0 #107# 81332608 COMMENT 100) (LOGPEN 0 0 16711680) 1 1 3.00958 1.9 COMMENT 0.082322 1) COMMENT #150=(CLabel (CWidget 0 (0 0) 1 2 0 0 #86# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT ""Arial"") 1.74233 0.666667 0 COMMENT ""pTC14 F2 primer"" ""@N"" 1 -6.31414 -19.1656 COMMENT 3.06854 17.391 7.14325 1.04901 #149#)) COMMENT (CObjectList)) COMMENT #151=(CGroupWidget COMMENT (CWidget 30 (7 30 0) 1 2 0 0 Nil -196 100) COMMENT (CObjectList COMMENT #152=(CPromoterArrow COMMENT (CLine COMMENT (CWidget 0 (3 #13# 0) 1 2 0 0 #108# 0 100) COMMENT (LOGPEN 0 4 128) 6 1.16464 5.72259 5.80986) COMMENT 0.4 0.082322 1.08464 1.8 1.8 0) COMMENT #153=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #86# 263585045 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT ""Arial"") 1.74233 0.666667 0 ""Tet promoter"" COMMENT ""@N"" 1 11.7659 10.454 -14.0998 5.99088 COMMENT 5.46983 1.04901 #152#)) (CObjectList))) COMMENT (CObjectList)) COMMENT #154=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -1904 100) COMMENT (CObjectList COMMENT #155=(CGroupWidget COMMENT (CWidget 1 (10 #0# 0) 1 2 0 0 Nil -2852 100) COMMENT (CObjectList COMMENT #156=(CScratch COMMENT (CWidget 1 (1 #0# 1) 1 2 0 0 #93# 81331488 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 1.66838 1.9 COMMENT 0.082322 1) COMMENT #157=(CLabel (CWidget 0 (0 0) 1 2 0 0 #95# 0 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT ""Georgia"") 1.74233 0.555556 128 COMMENT ""{\\i Apa}LI (2267)"" ""@N (@S)"" 5 -16.0297 0 COMMENT 8.81258 3.45752 4.32092 0.799245 #156#)) COMMENT (CObjectList)) COMMENT #158=(CGroupWidget COMMENT (CWidget 1 (10 #1# 0) 1 2 0 0 Nil 1620 100) COMMENT (CObjectList COMMENT #159=(CScratch COMMENT (CWidget 1 (1 #1# 1) 1 2 0 0 #93# 81338808 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 2.52187 1.9 COMMENT 0.082322 1) COMMENT #160=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #95# 38836800 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT ""Georgia"") 1.74233 0.555556 128 COMMENT ""{\\i Ava}I (1847)"" ""@N (@S)"" 5 -15.3322 COMMENT -3.48466 4.6701 5.99088 3.79641 0.799245 COMMENT #159#)) (CObjectList)) COMMENT #161=(CGroupWidget COMMENT (CWidget 1 (10 #3# 0) 1 2 0 0 Nil -196 100) COMMENT (CObjectList COMMENT #162=(CScratch COMMENT (CWidget 1 (1 #3# 1) 1 2 0 0 #93# 81332128 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 5.84644 1.9 COMMENT 0.082322 1) COMMENT #163=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #95# 11029 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT ""Georgia"") 1.74233 0.555556 128 COMMENT ""{\\i Cla}I (211)"" ""@N (@S)"" 5 8.24566 COMMENT 12.1963 -14.0613 4.7242 2.99717 0.799245 COMMENT #162#)) (CObjectList)) COMMENT #164=(CGroupWidget COMMENT (CWidget 1 (10 #6# 0) 1 2 0 0 Nil -3611 100) COMMENT (CObjectList COMMENT #165=(CScratch COMMENT (CWidget 1 (1 #6# 1) 1 2 0 0 #93# 81334288 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 2.54626 1.9 COMMENT 0.082322 1) COMMENT #166=(CLabel (CWidget 0 (0 0) 1 2 0 0 #95# 0 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT ""Georgia"") 1.74233 0.555556 128 COMMENT ""{\\i Nco}I (1835)"" ""@N (@S)"" 5 -14.0504 COMMENT -6.96932 4.50669 7.25756 3.69651 0.799245 COMMENT #165#)) (CObjectList)) COMMENT #167=(CGroupWidget COMMENT (CWidget 1 (10 #10# 0) 1 2 0 0 Nil -2093 100) COMMENT (CObjectList COMMENT #168=(CScratch COMMENT (CWidget 1 (1 #10# 1) 1 2 0 0 #93# 81335408 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 2.72102 1.9 COMMENT 0.082322 1) COMMENT #169=(CLabel (CWidget 0 (0 0) 1 2 0 0 #95# 0 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT ""Georgia"") 1.74233 0.555556 128 COMMENT ""{\\i Xba}I (1749)"" ""@N (@S)"" 5 -8.55358 COMMENT -13.9386 3.75438 13.591 3.89632 0.799245 COMMENT #168#)) (CObjectList)) COMMENT #170=(CGroupWidget COMMENT (CWidget 2 (10 #11# 0) 1 2 0 0 Nil -1797 100) COMMENT (CObjectList COMMENT #171=(CScratch COMMENT (CWidget 1 (1 #11# 1) 1 2 0 0 #93# 81332928 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 2.69664 1.9 COMMENT 0.082322 1) COMMENT #172=(CLabel (CWidget 0 (0 0) 1 2 0 0 #85# 211 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT ""Georgia"") 1.74233 0.555556 0 COMMENT ""{\\i Bsa}I (1761)"" ""@N (@S)"" 5 -8.61379 COMMENT -12.1963 3.76394 12.3243 3.54665 0.799245 COMMENT #171#) COMMENT #173=(CScratch COMMENT (CWidget 2 (1 #11# 2) 1 2 0 0 #93# 81334008 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 2.54626 1.9 COMMENT 0.082322 1) COMMENT #174=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #85# 50735156 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT ""Georgia"") 1.74233 0.555556 0 COMMENT ""{\\i Bsa}I (1835)"" ""@N (@S)"" 5 -14.7904 COMMENT -5.22699 4.50669 8.52424 3.62158 0.799245 COMMENT #173#)) (CObjectList))) (CObjectList)) COMMENT #175=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -1635 100) COMMENT (CObjectList) (CObjectList)) COMMENT #176=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -1228 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 51004096 COMMENT (CStringList) 0 0 (CObList))) FEATURES Location/Qualifiers source 1..3644 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1 /label=start /note="color: #0080ff" promoter 229..235 /label=Tet_ promoter /note="color: #ff8080" misc_feature 272..1462 /label=TetR /note="color: #0080ff" primer_bind 1607..1626 /label=pTC14_F2 primer /note="color: #8080ff; direction: RIGHT" misc_feature 1633 /label=end /note="color: #0080ff" primer_bind 1703..1724 /label=pTC14_R1 primer /note="color: #8080ff; direction: RIGHT" misc_feature 1754..1759 /note="BsaI site" /note="color: #0080ff" misc_feature 1761..1764 /note="4nt" /note="color: #0080ff" promoter 1765..2141 /label=OsU3 promoter /note="OsU3 promoter" /note="color: #ff8080" promoter 1765..1787 /label=AAGGGATCTTTAAACATACGAAC /note="pYPQ-13xD-F" /note="color: #ff8080" misc_structure 2143..2174 /note="guide RNA cloning site" /note="color: #0080ff" stem_loop 2175..2317 /note="guide RNA scaffold 2.0" /note="color: #00ff00" terminator 2318..2391 /label=OsU6-2 terminator /note="OsU6-2 terminator" /note="color: #000000" misc_feature complement(2369..2391) /label=AAGCTTATCGATACCCTCGACCT /note="pYPQ13xD-R" /note="color: #50ff50; direction: LEFT" misc_feature 2397..2402 /note="BsaI site" /note="color: #0080ff" misc_feature 2467..3140 /label=ori /note="color: #0080ff" primer_bind 3536..3555 /label=pTC14_F1 primer /note="color: #8080ff; direction: RIGHT" ORIGIN 1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 181 aattaattct catgtttgac agcttatcat cgattagctt taatgcggta gtttatcaca 241 gttaaattgc taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat 301 cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg 361 cctcttgcgg gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc 421 gctatatgcg ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt 481 tggccgccgc ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat 541 ggcgaccaca cccgtcctgt ggattctcta cgccggacgc atcgtggccg gcatcaccgg 601 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc 661 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc 721 cgggggactg ttgggcgcca tctccttaca tgcaccattc cttgcggcgg cggtgctcaa 781 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgccg 841 acccatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac 901 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc 961 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct 1021 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc 1081 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg 1141 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct 1201 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga 1261 cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat 1321 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc 1381 atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg 1441 gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact 1501 ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttga 1561 tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg 1621 agaaaataaa atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag 1681 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca 1741 tgcctcctct agaggtctcg ctataaggga tctttaaaca tacgaacaga tcacttaaag 1801 ttcttctgaa gcaacttaaa gttatcaggc atgcatggat cttggaggaa tcagatgtgc 1861 agtcagggac catagcacag gacaggcgtc ttctactggt gctaccagca aatgctggaa 1921 gccgggaaca ctgggtacgt tggaaaccac gtgatgtgga gtaagataaa ctgtaggaga 1981 aaagcatttc gtagtgggcc atgaagcctt tcaggacatg tattgcagta tgggccggcc 2041 cattacgcaa ttggacgaca acaaagacta gtattagtac cacctcggct atccacatag 2101 atcaaagctg gtttaaaaga gttgtgcaga tgatccgtgg cagagacgag atctagtctg 2161 agtcgaccgt ctctgtttta gagctaggcc aacatgagga tcacccatgt ctgcagggcc 2221 tagcaagtta aaataaggct agtccgttat caacttggcc aacatgagga tcacccatgt 2281 ctgcagggcc aagtggcacc gagtcggtgc tttttttgtc ccttcgaagg gcaattctgc 2341 agatatccat cacactggcg gccgctcgag gtcgagggta tcgataagct tcatgggaga 2401 ccctcgagcc acccatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg 2461 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 2521 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 2581 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt 2641 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 2701 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 2761 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 2821 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 2881 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 2941 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 3001 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 3061 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 3121 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt 3181 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag 3241 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg 3301 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc 3361 aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat 3421 ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc 3481 gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac 3541 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag 3601 cctttcgttt tatttgatgc ctggcagttc cctactctcg cgtt //