LOCUS       Exported                3644 bp ds-DNA     circular SYN 30-JUN-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pYPQ131D2.0
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 30, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
COMMENT     LOCUS pHD1 3087 bp DNA circular 10-DEC-2010 SOURCE ORGANISM COMMENT 
            This file is created by Vector NTI http://www.invitrogen.com/ 
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FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1
                     /label=start
                     /note="color: #0080ff"
     promoter        229..235
                     /label=Tet_ promoter
                     /note="color: #ff8080"
     misc_feature    272..1462
                     /label=TetR
                     /note="color: #0080ff"
     primer_bind     1607..1626
                     /label=pTC14_F2 primer
                     /note="color: #8080ff; direction: RIGHT"
     misc_feature    1633
                     /label=end
                     /note="color: #0080ff"
     primer_bind     1703..1724
                     /label=pTC14_R1 primer
                     /note="color: #8080ff; direction: RIGHT"
     misc_feature    1754..1759
                     /note="BsaI site"
                     /note="color: #0080ff"
     misc_feature    1761..1764
                     /note="4nt"
                     /note="color: #0080ff"
     promoter        1765..2141
                     /label=OsU3 promoter
                     /note="OsU3 promoter"
                     /note="color: #ff8080"
     promoter        1765..1787
                     /label=AAGGGATCTTTAAACATACGAAC
                     /note="pYPQ-13xD-F"
                     /note="color: #ff8080"
     misc_structure  2143..2174
                     /note="guide RNA cloning site"
                     /note="color: #0080ff"
     stem_loop       2175..2317
                     /note="guide RNA scaffold 2.0"
                     /note="color: #00ff00"
     terminator      2318..2391
                     /label=OsU6-2 terminator
                     /note="OsU6-2 terminator"
                     /note="color: #000000"
     misc_feature    complement(2369..2391)
                     /label=AAGCTTATCGATACCCTCGACCT
                     /note="pYPQ13xD-R"
                     /note="color: #50ff50; direction: LEFT"
     misc_feature    2397..2402
                     /note="BsaI site"
                     /note="color: #0080ff"
     misc_feature    2467..3140
                     /label=ori
                     /note="color: #0080ff"
     primer_bind     3536..3555
                     /label=pTC14_F1 primer
                     /note="color: #8080ff; direction: RIGHT"
ORIGIN
        1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
       61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      181 aattaattct catgtttgac agcttatcat cgattagctt taatgcggta gtttatcaca
      241 gttaaattgc taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat
      301 cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg
      361 cctcttgcgg gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc
      421 gctatatgcg ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt
      481 tggccgccgc ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat
      541 ggcgaccaca cccgtcctgt ggattctcta cgccggacgc atcgtggccg gcatcaccgg
      601 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
      661 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
      721 cgggggactg ttgggcgcca tctccttaca tgcaccattc cttgcggcgg cggtgctcaa
      781 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgccg
      841 acccatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
      901 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
      961 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct
     1021 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc
     1081 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg
     1141 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct
     1201 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga
     1261 cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat
     1321 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc
     1381 atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg
     1441 gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact
     1501 ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttga
     1561 tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg
     1621 agaaaataaa atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
     1681 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     1741 tgcctcctct agaggtctcg ctataaggga tctttaaaca tacgaacaga tcacttaaag
     1801 ttcttctgaa gcaacttaaa gttatcaggc atgcatggat cttggaggaa tcagatgtgc
     1861 agtcagggac catagcacag gacaggcgtc ttctactggt gctaccagca aatgctggaa
     1921 gccgggaaca ctgggtacgt tggaaaccac gtgatgtgga gtaagataaa ctgtaggaga
     1981 aaagcatttc gtagtgggcc atgaagcctt tcaggacatg tattgcagta tgggccggcc
     2041 cattacgcaa ttggacgaca acaaagacta gtattagtac cacctcggct atccacatag
     2101 atcaaagctg gtttaaaaga gttgtgcaga tgatccgtgg cagagacgag atctagtctg
     2161 agtcgaccgt ctctgtttta gagctaggcc aacatgagga tcacccatgt ctgcagggcc
     2221 tagcaagtta aaataaggct agtccgttat caacttggcc aacatgagga tcacccatgt
     2281 ctgcagggcc aagtggcacc gagtcggtgc tttttttgtc ccttcgaagg gcaattctgc
     2341 agatatccat cacactggcg gccgctcgag gtcgagggta tcgataagct tcatgggaga
     2401 ccctcgagcc acccatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg
     2461 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2521 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2581 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     2641 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     2701 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     2761 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     2821 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     2881 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     2941 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3001 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3061 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3121 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3181 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     3241 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     3301 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     3361 aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat
     3421 ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc
     3481 gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac
     3541 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag
     3601 cctttcgttt tatttgatgc ctggcagttc cctactctcg cgtt
//
