LOCUS       pAAV.hSyn.ChETA-EYFP.WPRE.hGH\(Addgene26967\MOD)\(P2417)        6235 bp    DNA     circular     19-AUG-2011
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|598873483|
COMMENT     VNTDBDATE|598873483|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV.hSyn.ChETA-EYFP.WPRE.hGH (Addgene26967 MOD) (P2417)|
COMMENT     VNTAUTHORNAME|Ruben Hovhannisyan|
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FEATURES             Location/Qualifiers
     mutation        1031..1033
                     /vntifkey="62"
                     /label=GAG\in\ChR2(H134R)
     CDS             1610..2326
                     /vntifkey="4"
                     /label=EYFP
     CDS             665..1609
                     /vntifkey="4"
                     /label=ChR2(H134R/E123T)
     mutation        1031..1033
                     /vntifkey="62"
                     /label=E123T
     LTR             1..130
                     /vntifkey="19"
                     /label=ITR
     LTR             complement(3487..3616)
                     /vntifkey="19"
                     /label=ITR
     CDS             4544..5404
                     /vntifkey="4"
                     /label=Amp-R
     misc_marker     2353..2940
                     /vntifkey="22"
                     /label=WPRE
     insertion_seq   2969..3447
                     /vntifkey="14"
                     /label=hGH\polyA
     misc_feature    3719..4025
                     /vntifkey="21"
                     /label=f1_origin
     misc_feature    5559..6178
                     /vntifkey="21"
                     /label=pBR322_origin
     promoter        149..621
                     /vntifkey="29"
                     /label=Synapsin\Promoter
BASE COUNT     1323 a      1761 c      1674 g      1477 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct gcggccgcac gcggatctct gcagagggcc ctgcgtatga gtgcaagtgg 
      181 gttttaggac caggatgagg cggggtgggg gtgcctacct gacgaccgac cccgacccac 
      241 tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag agggggaggg 
      301 gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg acagtgcctt 
      361 cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg ctgacgtcac 
      421 tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc gccgccgccg 
      481 gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc gaccatctgc 
      541 gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga ggagtcgtgt 
      601 cgtgcctgag agcgcagtcg aattcaagct gctagcaagg atcctctaga gtcgactagc 
      661 caccatggac tatggcggcg ctttgtctgc cgtcggacgc gaacttttgt tcgttactaa 
      721 tcctgtggtg gtgaacgggt ccgtcctggt ccctgaggat caatgttact gtgccggatg 
      781 gattgaatct cgcggcacga acggcgctca gaccgcgtca aatgtcctgc agtggcttgc 
      841 agcaggattc agcattttgc tgctgatgtt ctatgcctac caaacctgga aatctacatg 
      901 cggctgggag gagatctatg tgtgcgccat tgaaatggtt aaggtgattc tcgagttctt 
      961 ttttgagttt aagaatccct ctatgctcta ccttgccaca ggacaccggg tgcagtggct 
     1021 gcgctatgca acctggctgc tcacttgtcc tgtcatcctt atccgcctga gcaacctcac 
     1081 cggcctgagc aacgactaca gcaggagaac catgggactc cttgtctcag acatcgggac 
     1141 tatcgtgtgg ggggctacca gcgccatggc aaccggctat gttaaagtca tcttcttttg 
     1201 tcttggattg tgctatggcg cgaacacatt ttttcacgcc gccaaagcat atatcgaggg 
     1261 ttatcatact gtgccaaagg gtcggtgccg ccaggtcgtg accggcatgg catggctgtt 
     1321 tttcgtgagc tggggtatgt tcccaattct cttcattttg gggcccgaag gttttggcgt 
     1381 cctgagcgtc tatggctcca ccgtaggtca cacgattatt gatctgatga gtaaaaattg 
     1441 ttgggggttg ttgggacact acctgcgcgt cctgatccac gagcacatat tgattcacgg 
     1501 agatatccgc aaaaccacca aactgaacat cggcggaacg gagatcgagg tcgagactct 
     1561 cgtcgaagac gaagccgagg ccggagccgt gccagcggcc gccaccatgg tgagcaaggg 
     1621 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg 
     1681 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct 
     1741 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt 
     1801 cggctacggc ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt 
     1861 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg 
     1921 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga 
     1981 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa 
     2041 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa 
     2101 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca 
     2161 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca 
     2221 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt 
     2281 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaagaat tcgatatcaa 
     2341 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa 
     2401 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat 
     2461 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta 
     2521 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc 
     2581 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggaa ctttcgcttt 
     2641 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg 
     2701 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc 
     2761 ttggctgctc gcctatgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc 
     2821 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct 
     2881 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca 
     2941 tcgataccga gcgctgctcg agagatctac gggtggcatc cctgtgaccc ctccccagtg 
     3001 cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta 
     3061 agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg 
     3121 gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa 
     3181 ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa 
     3241 gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc 
     3301 agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc 
     3361 aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg 
     3421 tgaaccactg ctcccttccc tgtccttctg attttgtagg taaccacgtg cggaccgagc 
     3481 ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca 
     3541 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga 
     3601 gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc 
     3661 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc 
     3721 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 
     3781 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 
     3841 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 
     3901 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt 
     3961 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 
     4021 aacaacactc aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc 
     4081 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat 
     4141 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt 
     4201 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc 
     4261 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc 
     4321 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt 
     4381 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg 
     4441 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca 
     4501 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt 
     4561 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga 
     4621 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga 
     4681 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat 
     4741 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca 
     4801 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt 
     4861 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac 
     4921 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct 
     4981 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga 
     5041 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac 
     5101 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat 
     5161 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg 
     5221 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc 
     5281 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc 
     5341 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg 
     5401 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta 
     5461 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 
     5521 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 
     5581 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 
     5641 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 
     5701 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa 
     5761 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 
     5821 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 
     5881 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 
     5941 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 
     6001 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 
     6061 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 
     6121 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 
     6181 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtcctgca ggcag 
//
